DNASU Plasmid Repository • 480.965.5697 | Email

LRRC45 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

TOP

Explanation of Terms


Gene: Gene Symbol:  LRRC45
Gene Name:  leucine rich repeat containing 45
Sequence             
Original Clone ID: ccsbBroadEn_05197
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pENTR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: Original GenBank Accession: BC014109.2
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00514501

Price:  Login for Pricing
AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 2011nts         Open reading frame : 1 to 2011
Reference Sequence Alignment

ATGGAGGAGTTCCGGCGCTCCTACAGCCGCCTGTGCAGGGAGAGTGGGGCCGAGCCCCAG
GAGGCTGTCCTGCAGCAGCTGCACCAGCTTCCCAGGGGCCGGCTGGACCTGGCCACGCAA
AGCCTGACGGTGGAGACCTGCAGGGCCCTGGGCAAGCTGCTGCCGAGGGAGACGCTGTGC
ACGGAGCTGGTCCTGAGTGACTGCATGCTCAGCGAGGAAGGGGCCACACTGCTGCTCCGA
GGCCTGTGTGCCAACACCGTGCTGCGCTTTCTGGACTTAAAGGGCAACAACCTTCGGGCT
GCAGGGGCCGAGGCTCTGGGAAAACTCCTCCAACAGAACAAGTCCATTCAGAGCCTCACG
CTGGAGTGGAACAGCCTGGGCACGTGGGACGATGCCTTCGCCACCTTCTGCGGGGGCCTG
GCGGCCAACGGCGCCCTGCAGCGGCTGGACCTCCGCAACAACCAGATCAGTCACAAGGGC
GCGGAGGAGCTGGCCCTAGCCCTGAAGGGCAACACCACCCTCCAGCAGCTGGACCTGCGC
TGGAATAACGTTGGCCTCCTGGGGGGCCGGGCCCTCATGAACTGTCTCCCCAGCAACAGA
ACCCTGTGGAGACTGGACCTGGCTGGGAACAACATCCCTGGAGACGTCCTCAGAGCCGTG
GAGCAAGCCATGGGCCACAGCCAGGACCGGCTCACCACCTTCCAGGAGAACCAAGCCCGC
ACTCACGTCCTCAGCAAGGAGGTCCAGCACCTCCGGGAGGAGAAGTCCAAGCAGTTCCTC
GACTTGATGGAGACTATTGATAAGCAGCGAGAAGAGATGGCCAAGAGCAGCAGGGCGTCG
GCAGCCCGTGTAGGGCAGCTTCAGGAAGCCCTGAATGAGAGGCACTCCATCATCAACGCT
CTCAAGGCCAAGCTGCAGATGACAGAGGCCGCCCTGGCTCTGTCGGAGCAGAAGGCCCAG
GACCTGGGGGAGCTCCTGGCCACAGCGGAGCAGGAGCAGCTGAGCCTGTCACAGAGGCAG
GCCAAGGAGCTCAAGCTGGAGCAGCAGGAAGCTGCAGAGCGGGAGTCTAAACTCCTCAGA
GACTTGTCTGCTGCCAATGAAAAGAACCTGCTTCTGCAAAACCAGGTAGACGAGTTGGAG
CGGAAGTTCAGGTGTCAGCAGGAGCAGCTGTTCCAGACCAGGCAGGAGATGACCAGCATG
TCAGCTGAGCTGAAGATGCGGGCCATCCAGGCCGAGGAGCGCCTGGACATGGAGAAGAGA
AGATGCAGACAGAGCCTGGAGGACTCCGAAAGCCTGCGCATCAAGGAGGTGGAGCATATG
ACCCGTCACCTGGAGGAGAGTGAGAAGGCCATGCAGGAGCGGGTGCAGAGGCTGGAGGCG
GCGCGGCTGTCCCTGGAGGAGGAGCTGAGCCGAGTGAAAGCAGCGGCACTCAGCGAGCGT
GGCCAGGCTGAGGAGGAGCTGATCAAGGCCAAGAGCCAGGCCCGCCTGGAGGAGCAACAG
CGCCTGGCTCACCTGGAGGACAAGCTGAGACTGCTGGCGCAGGCACGGGACGAGGCGCAG
GGCGCTTGCCTACAGCAGAAGCAGGTGGTGGCCGAGGCCCAGACCCGGGTCAGCCAGCTG
GGCCTGCAAGTTGAGGGCCTGCGGCGGCGCCTGGAAGAGCTGCAGCAGGAGCTGAGCCTC
AAGGACCAGGAAAGGGTGGCCGAGGTGAGCAGGGTGCGCGTGGAGCTGCAGGAGCAGAAC
GGCCGGCTGCAGGCGGAGCTGGCGGCTCAGGAGGCGCTGAGGGAGAAGGCGGCGGCCCTG
GAGCGCCAGCTGAAAGTGATGGCGAGCGACCACCGAGAGGCGCTGCTGGACAGGGAGAGC
GAGAACGCGTCTCTCCGGGAGAAGCTGCGGCTCCGGGAGGCGGAGATCGCCCGCATCCGG
GACGAGGAGGCCCAGAGGGCGAGCTTCCTGCAGAACGCCGTCCTGGCTTACGTGCAGGCG
TCCCCCGTGAGGACCCTGAGCCCCCCAAAG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pENTR223

pENTR223 in advanced viewed

Synonyms: pDONR223, pENTR223-XnCDSYn
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp): 2815
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Mark Vidal
Publications: PMID: 15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 2220 2753
gene fragment pas 5p stem of pBR primose assembly site 63 73
gene fragment pas prime 3p stem of pBR pas(L) 105 116
recombination site attL1 attL1 recombination site 570 665
recombination site attL2 attL2 recombination site 793 696
selectable marker SpnR spectinomycin resistance gene 1062 2072
trxn termination sequence T1-T2 rrnBT1 and T2 terminators 510 224

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : LRRC45
Symbol Nomenclature : LRRC45
Designation : leucine-rich repeat-containing protein 45
Full Nomenclature : leucine rich repeat containing 45
GENEID : 201255
GenBank Accession : JF432130
HGNC : 28302
Vega : OTTHUMG00000178454
Target GenBank: JF432130

 

Reference Sequence Alignment

  

HsCD00514501      ATGGAGGAGTTCCGGCGCTCCTACAGCCGCCTGTGCAGGGAGAGTGGGGCCGAGCCCCAG
NM_144999.2       ATGGAGGAGTTCCGGCGCTCCTACAGCCGCCTGTGCAGGGAGAGTGGGGCCGAGCCCCAG
                  ************************************************************

HsCD00514501      GAGGCTGTCCTGCAGCAGCTGCACCAGCTTCCCAGGGGCCGGCTGGACCTGGCCACGCAA
NM_144999.2       GAGGCTGTCCTGCAGCAGCTGCACCAGCTTCCCAGGGGCCGGCTGGACCTGGCCACGCAA
                  ************************************************************

HsCD00514501      AGCCTGACGGTGGAGACCTGCAGGGCCCTGGGCAAGCTGCTGCCGAGGGAGACGCTGTGC
NM_144999.2       AGCCTGACGGTGGAGACCTGCAGGGCCCTGGGCAAGCTGCTGCCGAGGGAGACGCTGTGC
                  ************************************************************

HsCD00514501      ACGGAGCTGGTCCTGAGTGACTGCATGCTCAGCGAGGAAGGGGCCACACTGCTGCTCCGA
NM_144999.2       ACGGAGCTGGTCCTGAGTGACTGCATGCTCAGCGAGGAAGGGGCCACACTGCTGCTCCGA
                  ************************************************************

HsCD00514501      GGCCTGTGTGCCAACACCGTGCTGCGCTTTCTGGACTTAAAGGGCAACAACCTTCGGGCT
NM_144999.2       GGCCTGTGTGCCAACACCGTGCTGCGCTTTCTGGACTTAAAGGGCAACAACCTTCGGGCT
                  ************************************************************

HsCD00514501      GCAGGGGCCGAGGCTCTGGGAAAACTCCTCCAACAGAACAAGTCCATTCAGAGCCTCACG
NM_144999.2       GCAGGGGCCGAGGCTCTGGGAAAACTCCTCCAACAGAACAAGTCCATTCAGAGCCTCACG
                  ************************************************************

HsCD00514501      CTGGAGTGGAACAGCCTGGGCACGTGGGACGATGCCTTCGCCACCTTCTGCGGGGGCCTG
NM_144999.2       CTGGAGTGGAACAGCCTGGGCACGTGGGACGATGCCTTCGCCACCTTCTGCGGGGGCCTG
                  ************************************************************

HsCD00514501      GCGGCCAACGGCGCCCTGCAGCGGCTGGACCTCCGCAACAACCAGATCAGTCACAAGGGC
NM_144999.2       GCGGCCAACGGCGCCCTGCAGCGGCTGGACCTCCGCAACAACCAGATCAGTCACAAGGGC
                  ************************************************************

HsCD00514501      GCGGAGGAGCTGGCCCTAGCCCTGAAGGGCAACACCACCCTCCAGCAGCTGGACCTGCGC
NM_144999.2       GCGGAGGAGCTGGCCCTAGCCCTGAAGGGCAACACCACCCTCCAGCAGCTGGACCTGCGC
                  ************************************************************

HsCD00514501      TGGAATAACGTTGGCCTCCTGGGGGGCCGGGCCCTCATGAACTGTCTCCCCAGCAACAGA
NM_144999.2       TGGAATAACGTTGGCCTCCTGGGGGGCCGGGCCCTCATGAACTGTCTCCCCAGCAACAGA
                  ************************************************************

HsCD00514501      ACCCTGTGGAGACTGGACCTGGCTGGGAACAACATCCCTGGAGACGTCCTCAGAGCCGTG
NM_144999.2       ACCCTGTGGAGACTGGACCTGGCTGGGAACAACATCCCTGGAGACGTCCTCAGAGCCGTG
                  ************************************************************

HsCD00514501      GAGCAAGCCATGGGCCACAGCCAGGACCGGCTCACCACCTTCCAGGAGAACCAAGCCCGC
NM_144999.2       GAGCAAGCCATGGGCCACAGCCAGGACCGGCTCACCACCTTCCAGGAGAACCAAGCCCGC
                  ************************************************************

HsCD00514501      ACTCACGTCCTCAGCAAGGAGGTCCAGCACCTCCGGGAGGAGAAGTCCAAGCAGTTCCTC
NM_144999.2       ACTCACGTCCTCAGCAAGGAGGTCCAGCACCTCCGGGAGGAGAAGTCCAAGCAGTTCCTC
                  ************************************************************

HsCD00514501      GACTTGATGGAGACTATTGATAAGCAGCGAGAAGAGATGGCCAAGAGCAGCAGGGCGTCG
NM_144999.2       GACTTGATGGAGACTATTGATAAGCAGCGAGAAGAGATGGCCAAGAGCAGCAGGGCGTCG
                  ************************************************************

HsCD00514501      GCAGCCCGTGTAGGGCAGCTTCAGGAAGCCCTGAATGAGAGGCACTCCATCATCAACGCT
NM_144999.2       GCAGCCCGTGTAGGGCAGCTTCAGGAAGCCCTGAATGAGAGGCACTCCATCATCAACGCT
                  ************************************************************

HsCD00514501      CTCAAGGCCAAGCTGCAGATGACAGAGGCCGCCCTGGCTCTGTCGGAGCAGAAGGCCCAG
NM_144999.2       CTCAAGGCCAAGCTGCAGATGACAGAGGCCGCCCTGGCTCTGTCGGAGCAGAAGGCCCAG
                  ************************************************************

HsCD00514501      GACCTGGGGGAGCTCCTGGCCACAGCGGAGCAGGAGCAGCTGAGCCTGTCACAGAGGCAG
NM_144999.2       GACCTGGGGGAGCTCCTGGCCACAGCGGAGCAGGAGCAGCTGAGCCTGTCACAGAGGCAG
                  ************************************************************

HsCD00514501      GCCAAGGAGCTCAAGCTGGAGCAGCAGGAAGCTGCAGAGCGGGAGTCTAAACTCCTCAGA
NM_144999.2       GCCAAGGAGCTCAAGCTGGAGCAGCAGGAAGCTGCAGAGCGGGAGTCTAAACTCCTCAGA
                  ************************************************************

HsCD00514501      GACTTGTCTGCTGCCAATGAAAAGAACCTGCTTCTGCAAAACCAGGTAGACGAGTTGGAG
NM_144999.2       GACTTGTCTGCTGCCAATGAAAAGAACCTGCTTCTGCAAAACCAGGTAGACGAGTTGGAG
                  ************************************************************

HsCD00514501      CGGAAGTTCAGGTGTCAGCAGGAGCAGCTGTTCCAGACCAGGCAGGAGATGACCAGCATG
NM_144999.2       CGGAAGTTCAGGTGTCAGCAGGAGCAGCTGTTCCAGACCAGGCAGGAGATGACCAGCATG
                  ************************************************************

HsCD00514501      TCAGCTGAGCTGAAGATGCGGGCCATCCAGGCCGAGGAGCGCCTGGACATGGAGAAGAGA
NM_144999.2       TCAGCTGAGCTGAAGATGCGGGCCATCCAGGCCGAGGAGCGCCTGGACATGGAGAAGAGA
                  ************************************************************

HsCD00514501      AGATGCAGACAGAGCCTGGAGGACTCCGAAAGCCTGCGCATCAAGGAGGTGGAGCATATG
NM_144999.2       AGATGCAGACAGAGCCTGGAGGACTCCGAAAGCCTGCGCATCAAGGAGGTGGAGCATATG
                  ************************************************************

HsCD00514501      ACCCGTCACCTGGAGGAGAGTGAGAAGGCCATGCAGGAGCGGGTGCAGAGGCTGGAGGCG
NM_144999.2       ACCCGTCACCTGGAGGAGAGTGAGAAGGCCATGCAGGAGCGGGTGCAGAGGCTGGAGGCG
                  ************************************************************

HsCD00514501      GCGCGGCTGTCCCTGGAGGAGGAGCTGAGCCGAGTGAAAGCAGCGGCACTCAGCGAGCGT
NM_144999.2       GCGCGGCTGTCCCTGGAGGAGGAGCTGAGCCGAGTGAAAGCAGCGGCACTCAGCGAGCGT
                  ************************************************************

HsCD00514501      GGCCAGGCTGAGGAGGAGCTGATCAAGGCCAAGAGCCAGGCCCGCCTGGAGGAGCAACAG
NM_144999.2       GGCCAGGCTGAGGAGGAGCTGATCAAGGCCAAGAGCCAGGCCCGCCTGGAGGAGCAACAG
                  ************************************************************

HsCD00514501      CGCCTGGCTCACCTGGAGGACAAGCTGAGACTGCTGGCGCAGGCACGGGACGAGGCGCAG
NM_144999.2       CGCCTGGCTCACCTGGAGGACAAGCTGAGACTGCTGGCGCAGGCACGGGACGAGGCGCAG
                  ************************************************************

HsCD00514501      GGCGCTTGCCTACAGCAGAAGCAGGTGGTGGCCGAGGCCCAGACCCGGGTCAGCCAGCTG
NM_144999.2       GGCGCTTGCCTACAGCAGAAGCAGGTGGTGGCCGAGGCCCAGACCCGGGTCAGCCAGCTG
                  ************************************************************

HsCD00514501      GGCCTGCAAGTTGAGGGCCTGCGGCGGCGCCTGGAAGAGCTGCAGCAGGAGCTGAGCCTC
NM_144999.2       GGCCTGCAAGTTGAGGGCCTGCGGCGGCGCCTGGAAGAGCTGCAGCAGGAGCTGAGCCTC
                  ************************************************************

HsCD00514501      AAGGACCAGGAAAGGGTGGCCGAGGTGAGCAGGGTGCGCGTGGAGCTGCAGGAGCAGAAC
NM_144999.2       AAGGACCAGGAAAGGGTGGCCGAGGTGAGCAGGGTGCGCGTGGAGCTGCAGGAGCAGAAC
                  ************************************************************

HsCD00514501      GGCCGGCTGCAGGCGGAGCTGGCGGCTCAGGAGGCGCTGAGGGAGAAGGCGGCGGCCCTG
NM_144999.2       GGCCGGCTGCAGGCGGAGCTGGCGGCTCAGGAGGCGCTGAGGGAGAAGGCGGCGGCCCTG
                  ************************************************************

HsCD00514501      GAGCGCCAGCTGAAAGTGATGGCGAGCGACCACCGAGAGGCGCTGCTGGACAGGGAGAGC
NM_144999.2       GAGCGCCAGCTGAAAGTGATGGCGAGCGACCACCGAGAGGCGCTGCTGGACAGGGAGAGC
                  ************************************************************

HsCD00514501      GAGAACGCGTCTCTCCGGGAGAAGCTGCGGCTCCGGGAGGCGGAGATCGCCCGCATCCGG
NM_144999.2       GAGAACGCGTCTCTCCGGGAGAAGCTGCGGCTCCGGGAGGCGGAGATCGCCCGCATCCGG
                  ************************************************************

HsCD00514501      GACGAGGAGGCCCAGAGGGCGAGCTTCCTGCAGAACGCCGTCCTGGCTTACGTGCAGGCG
NM_144999.2       GACGAGGAGGCCCAGAGGGCGAGCTTCCTGCAGAACGCCGTCCTGGCTTACGTGCAGGCG
                  ************************************************************

HsCD00514501      TCCCCCGTGAGGACCCTGAGCCCCCCAAAG---
NM_144999.2       TCCCCCGTGAGGACCCTGAGCCCCCCAAAGTGA
                  ******************************   

Clone

Cloning Information : 605883
Original Clone ID : ccsbBroadEn_05197

Organism


TAX_ID : 9606
Species Specific ID: 201255

Genome

Chromosome : 17
Map Location : 17q25.3
Ensembl : ENSG00000169683

Reagents

Labome : LRRC45-antibody