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RNASE10 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  RNASE10
Gene Name:  ribonuclease, RNase A family, 10 (non-active)
Sequence             
Original Clone ID: ccsbBroadEn_05446
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pENTR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: Original GenBank Accession: BC133028.1
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00514996

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 649nts         Open reading frame : 1 to 649
Reference Sequence Alignment

ATGAAGCTGAATCTGGTGCAGATCTTTTTCATGTTGCTGATGCTGCTGCTGGGCCTGGGG
ATGGGCCTGGGGTTGGGACTTCATATGGCTACAGCAGTCTTGGAGGAGAGTGATCAACCG
CTCAATGAATTTTGGTCCAGTGACTCACAGGACAAAGCTGAGGCCACTGAGGAGGGAGAC
GGCACCCAAACCACAGAAACGCTGGTGCTTAGCAACAAAGAAGTGGTGCAACCTGGCTGG
CCAGAAGATCCCATCCTCGGTGAAGATGAGGTTGGGGGTAACAAGATGCTCAGAGCCTCA
GCTCTCTTTCAGAGCAACAAAGACTATCTTAGGCTTGACCAGACAGATAGAGAATGCAAT
GATATGATGGCACACAAGATGAAGGAGCCCAGTCAGAGTTGCATAGCCCAGTATGCATTC
ATCCATGAGGATCTAAACACAGTCAAAGCTGTCTGTAACAGTCCTGTCATTGCCTGTGAG
CTCAAGGGGGGAAAATGTCACAAAAGCTCCCGACCTTTTGATTTGACATTGTGCGAGCTG
TCCCAACCAGACCAGGTCACTCCTAACTGCAATTACCTAACTTCTGTTATAAAAAAGCAC
ATTATTATAACCTGTAATGACATGAAGCGCCAGTTACCAACTGGACAA


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pENTR223

pENTR223 in advanced viewed

Synonyms: pDONR223, pENTR223-XnCDSYn
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp): 2815
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Mark Vidal
Publications: PMID: 15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 2220 2753
gene fragment pas 5p stem of pBR primose assembly site 63 73
gene fragment pas prime 3p stem of pBR pas(L) 105 116
recombination site attL1 attL1 recombination site 570 665
recombination site attL2 attL2 recombination site 793 696
selectable marker SpnR spectinomycin resistance gene 1062 2072
trxn termination sequence T1-T2 rrnBT1 and T2 terminators 510 224

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : RNASE10
Symbol Nomenclature : RNASE10
SYNONYM : RAH1; RNASE9
Designation : inactive ribonuclease-like protein 10|ribonuclease A H1
Full Nomenclature : ribonuclease, RNase A family, 10 (non-active)
GENEID : 338879
HGNC : 19275
Vega : OTTHUMG00000170986
Target GenBank: None

 

Reference Sequence Alignment

  

HsCD00514996        ------------------------------------------------------------
XM_005267584.1      ATGTGGGGAGCACCTCTGCCCCGCCGCCCTGTCTGGGATGTGAGGAGCGCCTCTGCTGGG
NM_001012975.1      ------------------------------------------------------------
                                                                                

HsCD00514996        ------------------------ATGAAGCTGAATCTGGTGCAGATCTTTTTCATGTTG
XM_005267584.1      CCGCAACCCTGTCTGGGAGGCAAAATGAAGCTGAATCTGGTGCAGATCTTTTTCATGTTG
NM_001012975.1      ------------------------ATGAAGCTGAATCTGGTGCAGATCTTTTTCATGTTG
                                            ************************************

HsCD00514996        CTGATGCTGCTGCTGGGCCTGGGGATGGGCCTGGGGTTGGGACTTCATATGGCTACAGCA
XM_005267584.1      CTGATGCTGCTGCTGGGCCTGGGGATGGGCCTGGGGTTGGGACTTCATATGGCTACAGCA
NM_001012975.1      CTGATGCTGCTGCTGGGCCTGGGGATGGGCCTGGGGTTGGGACTTCATATGGCTACAGCA
                    ************************************************************

HsCD00514996        GTCTTGGAGGAGAGTGATCAACCGCTCAATGAATTTTGGTCCAGTGACTCACAGGACAAA
XM_005267584.1      GTCTTGGAGGAGAGTGATCAACCGCTCAATGAATTTTGGTCCAGTGACTCACAGGACAAA
NM_001012975.1      GTCTTGGAGGAGAGTGATCAACCGCTCAATGAATTTTGGTCCAGTGACTCACAGGACAAA
                    ************************************************************

HsCD00514996        GCTGAGGCCACTGAGGAGGGAGACGGCACCCAAACCACAGAAACGCTGGTGCTTAGCAAC
XM_005267584.1      GCTGAGGCCACTGAGGAGGGAGACGGCACCCAAACCACAGAAACGCTGGTGCTTAGCAAC
NM_001012975.1      GCTGAGGCCACTGAGGAGGGAGACGGCACCCAAACCACAGAAACGCTGGTGCTTAGCAAC
                    ************************************************************

HsCD00514996        AAAGAAGTGGTGCAACCTGGCTGGCCAGAAGATCCCATCCTCGGTGAAGATGAGGTTGGG
XM_005267584.1      AAAGAAGTGGTGCAACCTGGCTGGCCAGAAGATCCCATCCTCGGTGAAGATGAGGTTGGG
NM_001012975.1      AAAGAAGTGGTGCAACCTGGCTGGCCAGAAGATCCCATCCTCGGTGAAGATGAGGTTGGG
                    ************************************************************

HsCD00514996        GGTAACAAGATGCTCAGAGCCTCAGCTCTCTTTCAGAGCAACAAAGACTATCTTAGGCTT
XM_005267584.1      GGTAACAAGATGCTCAGAGCCTCAGCTCTCTTTCAGAGCAACAAAGACTATCTTAGGCTT
NM_001012975.1      GGTAACAAGATGCTCAGAGCCTCAGCTCTCTTTCAGAGCAACAAAGACTATCTTAGGCTT
                    ************************************************************

HsCD00514996        GACCAGACAGATAGAGAATGCAATGATATGATGGCACACAAGATGAAGGAGCCCAGTCAG
XM_005267584.1      GACCAGACAGATAGAGAATGCAATGATATGATGGCACACAAGATGAAGGAGCCCAGTCAG
NM_001012975.1      GACCAGACAGATAGAGAATGCAATGATATGATGGCACACAAGATGAAGGAGCCCAGTCAG
                    ************************************************************

HsCD00514996        AGTTGCATAGCCCAGTATGCATTCATCCATGAGGATCTAAACACAGTCAAAGCTGTCTGT
XM_005267584.1      AGTTGCATAGCCCAGTATGCATTCATCCATGAGGATCTAAACACAGTCAAAGCTGTCTGT
NM_001012975.1      AGTTGCATAGCCCAGTATGCATTCATCCATGAGGATCTAAACACAGTCAAAGCTGTCTGT
                    ************************************************************

HsCD00514996        AACAGTCCTGTCATTGCCTGTGAGCTCAAGGGGGGAAAATGTCACAAAAGCTCCCGACCT
XM_005267584.1      AACAGTCCTGTCATTGCCTGTGAGCTCAAGGGGGGAAAATGTCACAAAAGCTCCCGACCT
NM_001012975.1      AACAGTCCTGTCATTGCCTGTGAGCTCAAGGGGGGAAAATGTCACAAAAGCTCCCGACCT
                    ************************************************************

HsCD00514996        TTTGATTTGACATTGTGCGAGCTGTCCCAACCAGACCAGGTCACTCCTAACTGCAATTAC
XM_005267584.1      TTTGATTTGACATTGTGCGAGCTGTCCCAACCAGACCAGGTCACTCCTAACTGCAATTAC
NM_001012975.1      TTTGATTTGACATTGTGCGAGCTGTCCCAACCAGACCAGGTCACTCCTAACTGCAATTAC
                    ************************************************************

HsCD00514996        CTAACTTCTGTTATAAAAAAGCACATTATTATAACCTGTAATGACATGAAGCGCCAGTTA
XM_005267584.1      CTAACTTCTGTTATAAAAAAGCACATTATTATAACCTGTAATGACATGAAGCGCCAGTTA
NM_001012975.1      CTAACTTCTGTTATAAAAAAGCACATTATTATAACCTGTAATGACATGAAGCGCCAGTTA
                    ************************************************************

HsCD00514996        CCAACTGGACAA---
XM_005267584.1      CCAACTGGACAATGA
NM_001012975.1      CCAACTGGACAATGA
                    ************   

Protein

SMART domain : RNAse_Pc : Pancreatic ribonuclease
HPRD : 18495

Clone

Cloning Information : 665670
Original Clone ID : ccsbBroadEn_05446

Organism


TAX_ID : 9606
Species Specific ID: 338879

Genome

Chromosome : 14
Map Location : 14q11.2
Ensembl : ENSG00000182545

Reagents

Labome : RNASE10-antibody