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TCEA1 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  TCEA1
Gene Name:  transcription elongation factor A (SII), 1
Sequence             
Original Clone ID: ccsbBroadEn_01650
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pENTR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: Original GenBank Accession: BC072460.1
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00515189

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 904nts        
Open reading frame : 1 to 904
Reference Sequence Alignment

ATGGAGGACGAAGTGGTCCGCTTTGCCAAGAAGATGGACAAGATGGTGCAGAAGAAGAAC
GCGGCTGGAGCATTGGATTTGCTAAAGGAGCTTAAGAATATTCCTATGACCCTGGAATTA
CTGCAGTCCACAAGAATCGGAATGTCAGTTAATGCTATTCGCAAGCAGAGTACAGATGAG
GAAGTTACATCTTTGGCAAAGTCTCTCATCAAATCCTGGAAAAAATTATTAGATGGGCCA
TCAACTGAGAAAGACCTTGACGAAAAGAAGAAAGAACCTGCAATTACATCGCAGAACAGC
CCTGAGGCAAGAGAAGAAAGTACTTCCAGCGGCAATGTAAGCAACAGAAAGGATGAGACA
AATGCTCGAGATACTTATGTTTCATCCTTTCCTCGGGCACCAAGCACTTCTGATTCTGTG
CGGTTGAAGTGTAGGGAGATGCTTGCTGCAGCTCTTCGAACAGGGGATGACTACATTGCA
ATTGGAGCTGATGAGGAAGAATTAGGATCTCAAATTGAAGAAGCTATATATCAAGAAATA
AGGAATACAGACATGAAATACAAAAATAGAGTACGAAGTAGGATATCAAATCTTAAAGAT
GCAAAAAATCCAAATTTAAGGAAAAATGTCCTCTGTGGGAATATTCCTCCTGACTTATTT
GCTAGAATGACAGCAGAGGAAATGGCTAGTGATGAGCTGAAAGAGATGCGGAAAAACTTG
ACCAAAGAAGCCATCAGAGAGCATCAGATGGCCAAGACTGGTGGGACCCAGACTGACTTG
TTCACATGTGGCAAATGTAAAAAGAAGAATTGCACTTACACACAGGTACAAACCCGTAGT
GCTGATGAACCAATGACAACATTTGTTGTCTGTAATGAATGTGGAAATCGATGGAAGTTC
TGT


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pENTR223

pENTR223 in advanced viewed

Synonyms: pDONR223, pENTR223-XnCDSYn
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp): 2815
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Mark Vidal
Publications: PMID: 15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 2220 2753
gene fragment pas 5p stem of pBR primose assembly site 63 73
gene fragment pas prime 3p stem of pBR pas(L) 105 116
recombination site attL1 attL1 recombination site 570 665
recombination site attL2 attL2 recombination site 793 696
selectable marker SpnR spectinomycin resistance gene 1062 2072
trxn termination sequence T1-T2 rrnBT1 and T2 terminators 510 224

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : TCEA1
Symbol Nomenclature : TCEA1
SYNONYM : GTF2S; SII; TCEA; TF2S; TFIIS
Designation : transcription elongation factor A protein 1|transcription elongation factor S-II protein 1|transcription elongation factor TFIIS.o
Full Nomenclature : transcription elongation factor A (SII), 1
GENEID : 6917
HGNC : 11612
MIM : 601425
Vega : OTTHUMG00000164262
Target GenBank: None

 

Reference Sequence Alignment

  

HsCD00515189      ATGGAGGACGAAGTGGTCCGCTTTGCCAAGAAGATGGACAAGATGGTGCAGAAGAAGAAC
NM_006756.3       ATGGAGGACGAAGTGGTCCGCTTTGCCAAGAAGATGGACAAGATGGTGCAGAAGAAGAAC
NM_201437.2       ATGGAGGACGAAGTGGTCCGCTTTGCCAAGAAGATGGACAAGATGGTGCAGAAGAAGAAC
                  ************************************************************

HsCD00515189      GCGGCTGGAGCATTGGATTTGCTAAAGGAGCTTAAGAATATTCCTATGACCCTGGAATTA
NM_006756.3       GCGGCTGGAGCATTGGATTTGCTAAAGGAGCTTAAGAATATTCCTATGACCCTGGAATTA
NM_201437.2       GCG---------------------------------------------------------
                  ***                                                         

HsCD00515189      CTGCAGTCCACAAGAATCGGAATGTCAGTTAATGCTATTCGCAAGCAGAGTACAGATGAG
NM_006756.3       CTGCAGTCCACAAGAATCGGAATGTCAGTTAATGCTATTCGCAAGCAGAGTACAGATGAG
NM_201437.2       ------TCCACAAGAATCGGAATGTCAGTTAATGCTATTCGCAAGCAGAGTACAGATGAG
                        ******************************************************

HsCD00515189      GAAGTTACATCTTTGGCAAAGTCTCTCATCAAATCCTGGAAAAAATTATTAGATGGGCCA
NM_006756.3       GAAGTTACATCTTTGGCAAAGTCTCTCATCAAATCCTGGAAAAAATTATTAGATGGGCCA
NM_201437.2       GAAGTTACATCTTTGGCAAAGTCTCTCATCAAATCCTGGAAAAAATTATTAGATGGGCCA
                  ************************************************************

HsCD00515189      TCAACTGAGAAAGACCTTGACGAAAAGAAGAAAGAACCTGCAATTACATCGCAGAACAGC
NM_006756.3       TCAACTGAGAAAGACCTTGACGAAAAGAAGAAAGAACCTGCAATTACATCGCAGAACAGC
NM_201437.2       TCAACTGAGAAAGACCTTGACGAAAAGAAGAAAGAACCTGCAATTACATCGCAGAACAGC
                  ************************************************************

HsCD00515189      CCTGAGGCAAGAGAAGAAAGTACTTCCAGCGGCAATGTAAGCAACAGAAAGGATGAGACA
NM_006756.3       CCTGAGGCAAGAGAAGAAAGTACTTCCAGCGGCAATGTAAGCAACAGAAAGGATGAGACA
NM_201437.2       CCTGAGGCAAGAGAAGAAAGTACTTCCAGCGGCAATGTAAGCAACAGAAAGGATGAGACA
                  ************************************************************

HsCD00515189      AATGCTCGAGATACTTATGTTTCATCCTTTCCTCGGGCACCAAGCACTTCTGATTCTGTG
NM_006756.3       AATGCTCGAGATACTTATGTTTCATCCTTTCCTCGGGCACCAAGCACTTCTGATTCTGTG
NM_201437.2       AATGCTCGAGATACTTATGTTTCATCCTTTCCTCGGGCACCAAGCACTTCTGATTCTGTG
                  ************************************************************

HsCD00515189      CGGTTGAAGTGTAGGGAGATGCTTGCTGCAGCTCTTCGAACAGGGGATGACTACATTGCA
NM_006756.3       CGGTTGAAGTGTAGGGAGATGCTTGCTGCAGCTCTTCGAACAGGGGATGACTACATTGCA
NM_201437.2       CGGTTGAAGTGTAGGGAGATGCTTGCTGCAGCTCTTCGAACAGGGGATGACTACATTGCA
                  ************************************************************

HsCD00515189      ATTGGAGCTGATGAGGAAGAATTAGGATCTCAAATTGAAGAAGCTATATATCAAGAAATA
NM_006756.3       ATTGGAGCTGATGAGGAAGAATTAGGATCTCAAATTGAAGAAGCTATATATCAAGAAATA
NM_201437.2       ATTGGAGCTGATGAGGAAGAATTAGGATCTCAAATTGAAGAAGCTATATATCAAGAAATA
                  ************************************************************

HsCD00515189      AGGAATACAGACATGAAATACAAAAATAGAGTACGAAGTAGGATATCAAATCTTAAAGAT
NM_006756.3       AGGAATACAGACATGAAATACAAAAATAGAGTACGAAGTAGGATATCAAATCTTAAAGAT
NM_201437.2       AGGAATACAGACATGAAATACAAAAATAGAGTACGAAGTAGGATATCAAATCTTAAAGAT
                  ************************************************************

HsCD00515189      GCAAAAAATCCAAATTTAAGGAAAAATGTCCTCTGTGGGAATATTCCTCCTGACTTATTT
NM_006756.3       GCAAAAAATCCAAATTTAAGGAAAAATGTCCTCTGTGGGAATATTCCTCCTGACTTATTT
NM_201437.2       GCAAAAAATCCAAATTTAAGGAAAAATGTCCTCTGTGGGAATATTCCTCCTGACTTATTT
                  ************************************************************

HsCD00515189      GCTAGAATGACAGCAGAGGAAATGGCTAGTGATGAGCTGAAAGAGATGCGGAAAAACTTG
NM_006756.3       GCTAGAATGACAGCAGAGGAAATGGCTAGTGATGAGCTGAAAGAGATGCGGAAAAACTTG
NM_201437.2       GCTAGAATGACAGCAGAGGAAATGGCTAGTGATGAGCTGAAAGAGATGCGGAAAAACTTG
                  ************************************************************

HsCD00515189      ACCAAAGAAGCCATCAGAGAGCATCAGATGGCCAAGACTGGTGGGACCCAGACTGACTTG
NM_006756.3       ACCAAAGAAGCCATCAGAGAGCATCAGATGGCCAAGACTGGTGGGACCCAGACTGACTTG
NM_201437.2       ACCAAAGAAGCCATCAGAGAGCATCAGATGGCCAAGACTGGTGGGACCCAGACTGACTTG
                  ************************************************************

HsCD00515189      TTCACATGTGGCAAATGTAAAAAGAAGAATTGCACTTACACACAGGTACAAACCCGTAGT
NM_006756.3       TTCACATGTGGCAAATGTAAAAAGAAGAATTGCACTTACACACAGGTACAAACCCGTAGT
NM_201437.2       TTCACATGTGGCAAATGTAAAAAGAAGAATTGCACTTACACACAGGTACAAACCCGTAGT
                  ************************************************************

HsCD00515189      GCTGATGAACCAATGACAACATTTGTTGTCTGTAATGAATGTGGAAATCGATGGAAGTTC
NM_006756.3       GCTGATGAACCAATGACAACATTTGTTGTCTGTAATGAATGTGGAAATCGATGGAAGTTC
NM_201437.2       GCTGATGAACCAATGACAACATTTGTTGTCTGTAATGAATGTGGAAATCGATGGAAGTTC
                  ************************************************************

HsCD00515189      TGT---
NM_006756.3       TGTTGA
NM_201437.2       TGTTGA
                  ***   

Pathways

Reactome : Disease
Reactome : DNA Repair
Reactome : Dual incision reaction in TC-NER
Reactome : Elongation arrest and recovery
Reactome : Formation of HIV elongation complex in the absence of HIV Tat
Reactome : Formation of HIV-1 elongation complex containing HIV-1 Tat
Reactome : Formation of RNA Pol II elongation complex
Reactome : Formation of transcription-coupled NER (TC-NER) repair complex
Reactome : Gene Expression
Reactome : HIV elongation arrest and recovery
Reactome : HIV Infection
Reactome : HIV Life Cycle
Reactome : HIV Transcription Elongation
Reactome : Late Phase of HIV Life Cycle
Reactome : Nucleotide Excision Repair
Reactome : Pausing and recovery of HIV elongation
Reactome : Pausing and recovery of Tat-mediated HIV elongation
Reactome : RNA Polymerase II Pre-transcription Events
Reactome : RNA Polymerase II Transcription
Reactome : RNA Polymerase II Transcription Elongation
Reactome : Tat-mediated elongation of the HIV-1 transcript
Reactome : Tat-mediated HIV elongation arrest and recovery
Reactome : Transcription
Reactome : Transcription of the HIV genome
Reactome : Transcription-coupled NER (TC-NER)

Clone

Cloning Information : 608871
Original Clone ID : ccsbBroadEn_01650

Organism


TAX_ID : 9606
Species Specific ID: 6917

Genome

Chromosome : 8
Map Location : 8q11.2
Ensembl : ENSG00000187735

Reagents

Labome : TCEA1-antibody