DNASU Plasmid | KCTD10 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: KCTD10

Gene Name: potassium channel tetramerization domain containing 10

Original Clone ID:

ccsbBroadEn_09130

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC040062.1

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00515252

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 940nts

Open reading frame: 1 to 940

Reference Sequence Alignment

ATGGAAGAGATGTCAGGAGAAAGTGTGGTGAGCTCAGCGGTGCCAGCGGCTGCTACCCGC
ACCACTTCCTTCAAGGGCACGAGCCCCAGCTCCAAATACGTGAAGCTGAATGTGGGTGGA
GCCCTCTACTATACCACCATGCAGACGCTGACCAAGCAGGACACCATGCTGAAGGCCATG
TTCAGCGGGCGCATGGAAGTGCTCACCGACAGTGAAGGCTGGATCCTCATTGACCGCTGT
GGGAAGCACTTTGGTACGATACTCAACTACCTTCGAGACGGGGCGGTGCCTTTACCCGAG
AGCCGCCGGGAGATCGAGGAGCTGCTAGCAGAAGCCAAGTACTACCTAGTCCAAGGCCTG
GTGGAAGAGTGCCAGGCGGCCCTACAAAACAAAGATACTTATGAGCCTTTCTGCAAGGTC
CCTGTGATCACCTCATCCAAGGAAGAACAAAAACTTATAGCGACTTCAAATAAGCCAGCC
GTGAAGTTGCTCTACAACAGAAGTAACAACAAATACTCATATACCAGCAATTCTGACGAC
AATATGTTGAAAAACATTGAACTGTTTGATAAGCTGTCTCTGCGCTTTAACGGAAGGGTC
CTGTTCATAAAGGATGTCATTGGGGATGAAATCTGCTGCTGGTCCTTTTATGGTCAGGGC
CGGAAGATTGCTGAAGTCTGTTGTACCTCCATCGTCTATGCCACTGAGAAGAAACAGACC
AAGGTGGAGTTTCCCGAAGCCCGGATTTATGAGGAGACCCTGAACATTTTGCTGTATGAG
GCCCAGGATGGCCGGGGACCTGACAATGCGCTCCTGGAGGCCACAGGCGGGGCGGCGGGG
CGCTCCCACCACCTGGACGAGGACGAGGAGCGGGAGCGGATCGAGCGCGTGCGGAGGATC
CACATCAAGCGCCCTGATGACCGGGCCCACCTCCACCAG

Protein sequence predicted based on nucleotide sequence

MEEMSGESVVSSAVPAAATRTTSFKGTSPSSKYVKLNVGGALYYTTMQTLTKQDTMLKAM
FSGRMEVLTDSEGWILIDRCGKHFGTILNYLRDGAVPLPESRREIEELLAEAKYYLVQGL
VEECQAALQNKDTYEPFCKVPVITSSKEEQKLIATSNKPAVKLLYNRSNNKYSYTSNSDD
NMLKNIELFDKLSLRFNGRVLFIKDVIGDEICCWSFYGQGRKIAEVCCTSIVYATEKKQT
KVEFPEARIYEETLNILLYEAQDGRGPDNALLEATGGAAGRSHHLDEDEERERIERVRRI
HIKRPDDRAHLHQ

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

KCTD10

Symbol Nomenclature :

KCTD10

SYNONYM :

BTBD28; ULRO61; hBACURD3

Designation :

BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 3|BTB/POZ domain-containing protein KCTD10|potassium channel tetramerisation domain containing 10|potassium channel tetramerization domain-containing protein 10

Full Nomenclature :

potassium channel tetramerization domain containing 10

GENEID :

83892

Locus Tag :

MSTP028

HGNC :

23236

MIM :

613421

Vega :

OTTHUMG00000169253

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00515252        ATGGAAGAGATGTCAGGAGAAAGTGTGGTGAGCTCAGCGGTGCCAGCGGCTGCTACCCGC
NM_031954.3         ATGGAAGAGATGTCAGGAGAAAGTGTGGTGAGCTCAGCGGTGCCAGCGGCTGCTACCCGC
XM_005253946.1      ATGGAAGAGATGTCAGGAGAAAGTGTGGTGAGCTCAGCGGTGCCAGCGGCTGCTACCCGC
                    ************************************************************

HsCD00515252        ACCACTTCCTTCAAGGGCACGAGCCCCAGCTCCAAATACGTGAAGCTGAATGTGGGTGGA
NM_031954.3         ACCACTTCCTTCAAGGGCACGAGCCCCAGCTCCAAATACGTGAAGCTGAATGTGGGTGGA
XM_005253946.1      ACCACTTCCTTCAAGGGCACGAGCCCCAGCTCCAAATACGTGAAGCTGAATGTGGGTGGA
                    ************************************************************

HsCD00515252        GCCCTCTACTATACCACCATGCAGACGCTGACCAAGCAGGACACCATGCTGAAGGCCATG
NM_031954.3         GCCCTCTACTATACCACCATGCAGACGCTGACCAAGCAGGACACCATGCTGAAGGCCATG
XM_005253946.1      GCCCTCTACTATACCACCATGCAGACGCTGACCAAGCAGGACACCATGCTGAAGGCCATG
                    ************************************************************

HsCD00515252        TTCAGCGGGCGCATGGAAGTGCTCACCGACAGTGAAGGCTGGATCCTCATTGACCGCTGT
NM_031954.3         TTCAGCGGGCGCATGGAAGTGCTCACCGACAGTGAAGGCTGGATCCTCATTGACCGCTGT
XM_005253946.1      TTCAGCGGGCGCATGGAAGTGCTCACCGACAGTGAAGGCTGGATCCTCATTGACCGCTGT
                    ************************************************************

HsCD00515252        GGGAAGCACTTTGGTACGATACTCAACTACCTTCGAGACGGGGCGGTGCCTTTACCCGAG
NM_031954.3         GGGAAGCACTTTGGTACGATACTCAACTACCTTCGAGACGGGGCGGTGCCTTTACCCGAG
XM_005253946.1      GGGAAGCACTTTGGTACGATACTCAACTACCTTCGAGACGGGGCGGTGCCTTTACCCGAG
                    ************************************************************

HsCD00515252        AGCCGCCGGGAGATCGAGGAGCTGCTAGCAGAAGCCAAGTACTACCTAGTCCAAGGCCTG
NM_031954.3         AGCCGCCGGGAGATCGAGGAGCTGCTAGCAGAAGCCAAGTACTACCTAGTCCAAGGCCTG
XM_005253946.1      AGCCGCCGGGAGATCGAGGAGCTGCTAGCAGAAGCCAAGTACTACCTAGTCCAAGGCCTG
                    ************************************************************

HsCD00515252        GTGGAAGAGTGCCAGGCG------GCCCTACAAAACAAAGATACTTATGAGCCTTTCTGC
NM_031954.3         GTGGAAGAGTGCCAG---GCG---GCCCTACAAAACAAAGATACTTATGAGCCTTTCTGC
XM_005253946.1      GTGGAAGAGTGCCAG---GCGGCCCTACAACAGAACAAAGATACTTATGAGCCTTTCTGC
                    ***************           .*:***.***************************

HsCD00515252        AAGGTCCCTGTGATCACCTCATCCAAGGAAGAACAAAAACTTATAGCGACTTCAAATAAG
NM_031954.3         AAGGTCCCTGTGATCACCTCATCCAAGGAAGAACAAAAACTTATAGCGACTTCAAATAAG
XM_005253946.1      AAGGTCCCTGTGATCACCTCATCCAAGGAAGAACAAAAACTTATAGCGACTTCAAATAAG
                    ************************************************************

HsCD00515252        CCAGCCGTGAAGTTGCTCTACAACAGAAGTAACAACAAATACTCATATACCAGCAATTCT
NM_031954.3         CCAGCCGTGAAGTTGCTCTACAACAGAAGTAACAACAAATACTCATATACCAGCAATTCT
XM_005253946.1      CCAGCCGTGAAGTTGCTCTACAACAGAAGTAACAACAAATACTCATATACCAGCAATTCT
                    ************************************************************

HsCD00515252        GACGACAATATGTTGAAAAACATTGAACTGTTTGATAAGCTGTCTCTGCGCTTTAACGGA
NM_031954.3         GACGACAATATGTTGAAAAACATTGAACTGTTTGATAAGCTGTCTCTGCGCTTTAACGGA
XM_005253946.1      GACGACAATATGTTGAAAAACATTGAACTGTTTGATAAGCTGTCTCTGCGCTTTAACGGA
                    ************************************************************

HsCD00515252        AGGGTCCTGTTCATAAAGGATGTCATTGGGGATGAAATCTGCTGCTGGTCCTTTTATGGT
NM_031954.3         AGGGTCCTGTTCATAAAGGATGTTATTGGGGATGAAATCTGCTGCTGGTCCTTTTATGGT
XM_005253946.1      AGGGTCCTGTTCATAAAGGATGTTATTGGGGATGAAATCTGCTGCTGGTCCTTTTATGGT
                    *********************** ************************************

HsCD00515252        CAGGGCCGGAAGATTGCTGAAGTCTGTTGTACCTCCATCGTCTATGCCACTGAGAAGAAA
NM_031954.3         CAGGGCCGGAAGATTGCTGAAGTCTGTTGTACCTCCATCGTCTATGCCACTGAGAAGAAA
XM_005253946.1      CAGGGCCGGAAGATTGCTGAAGTCTGTTGTACCTCCATCGTCTATGCCACTGAGAAGAAA
                    ************************************************************

HsCD00515252        CAGACCAAGGTGGAGTTTCCCGAAGCCCGGATTTATGAGGAGACCCTGAACATTTTGCTG
NM_031954.3         CAGACCAAGGTGGAGTTTCCCGAAGCCCGGATTTATGAGGAGACCCTGAACATTTTGCTG
XM_005253946.1      CAGACCAAGGTGGAGTTTCCCGAAGCCCGGATTTATGAGGAGACCCTGAACATTTTGCTG
                    ************************************************************

HsCD00515252        TATGAGGCCCAGGATGGCCGGGGACCTGACAATGCGCTCCTGGAGGCCACAGGCGGGGCG
NM_031954.3         TATGAGGCCCAGGATGGCCGGGGACCTGACAATGCGCTCCTGGAGGCCACAGGCGGGGCG
XM_005253946.1      TATGAGGCCCAGGATGGCCGGGGACCTGACAATGCGCTCCTGGAGGCCACAGGCGGGGCG
                    ************************************************************

HsCD00515252        GCGGGGCGCTCCCACCACCTGGACGAGGACGAGGAGCGGGAGCGGATCGAGCGCGTGCGG
NM_031954.3         GCGGGGCGCTCCCACCACCTGGACGAGGACGAGGAGCGGGAGCGGATCGAGCGCGTGCGG
XM_005253946.1      GCGGGGCGCTCCCACCACCTGGACGAGGACGAGGAGCGGGAGCGGATCGAGCGCGTGCGG
                    ************************************************************

HsCD00515252        AGGATCCACATCAAGCGCCCTGATGACCGGGCCCACCTCCACCAG---
NM_031954.3         AGGATCCACATCAAGCGCCCTGATGACCGGGCCCACCTCCACCAGTGA
XM_005253946.1      AGGATCCACATCAAGCGCCCTGATGACCGGGCCCACCTCCACCAGTGA
                    *********************************************