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ZRSR2 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  ZRSR2
Gene Name:  zinc finger (CCCH type), RNA-binding motif and serine/arginine rich 2
Sequence             
Original Clone ID: ccsbBroadEn_07211
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pENTR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: Original GenBank Accession: BC050451.1
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00515826

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1447nts         Open reading frame : 1 to 1447

ATGGCTGCGCCCGAGAAGATGACGTTTCCCGAGAAACCAAGCCACAAAAAGTACAGGGCC
GCCCTGAAGAAGGAGAAACGAAAGAAACGTCGGCAGGAACTTGCTCGACTGAGAGACTCA
GGACTCTCACAGAAGGAGGAAGAGGAGGACACTTTTATTGAAGAACAACAACTAGAAGAA
GAGAAGCTATTGGAAAGAGAGAGGCAAAGATTACATGAGGAGTGGTTGCTAAGAGAGCAG
AAGGCACAAGAAGAATTCAGAATAAAGAAGGAAAAGGAAGAGGCGGCTAAAAAACGGCAA
GAAGAACAAGAGAGAAAGTTAAAGGAACAATGGGAAGAACAGCAGAGGAAAGAGAGAGAA
GAGGAGGAGCAGAAACGACAGGAGAAGAAAGAAAAAGAGGAAGCTTTGCAGAAGATGCTG
GATCAGGCTGAAAATGAGTTGGAAAATGGTACCACATGGCAAAACCCAGAACCACCCGTG
GATTTCAGAGTAATGGAGAAGGATCGAGCTAATTGTCCCTTCTACAGTAAAACAGGAGCT
TGCAGATTTGGAGATAGATGTTCACGTAAACATAATTTCCCAACATCCAGTCCTACCCTT
CTTATTAAGAGCATGTTTACGACGTTTGGAATGGAGCAGTGCAGGAGGGATGACTATGAC
CCTGACGCAAGCCTGGAGTACAGCGAGGAAGAAACCTACCAACAGTTCCTAGACTTCTAT
GAGGATGTGTTGCCCGAGTTCAAGAACGTGGGGAAAGTGATTCAGTTCAAGGTCAGCTGC
AATTTGGAACCTCACCTGAGGGGCAATGTATATGTTCAGTACCAGTCGGAAGAAGAATGC
CAAGCAGCCCTTTCTCTGTTTAACGGACGATGGTATGCAGGACGACAGCTGCAGTGTGAA
TTCTGCCCCGTGACCCGGTGGAAAATGGCGATTTGTGGTTTATTTGAAATACAACAATGT
CCAAGAGGAAAGCACTGCAACTTTCTTCATGTGTTCAGAAATCCCAACAATGAATTCTGG
GAAGCTAATAGAGACATCTACTTGTCTCCAGATCGGACTGGCTCCTCCTTTGGGAAGAAC
TCCGAAAGGAGGGAGAGGATGGGCCACCACGACGACTACTACAGCAGGCTGCGGGGAAGG
AGAAACCCTAGTCCAGACCACTCCTACAAAAGAAATGGGGAATCCGAGAGGAAAAGTAGT
CGTCACAGGGGGAAGAAATCTCACAAACGCACATCAAAGAGTCGGGAGAGGCACAATTCA
CGAAGCAGAGGAAGAAATAGGGACCGCAGCAGGGACCGCAGCCGGGGCCGGGGCAGCCGG
AGCCGGAGCCGGAGCCGGAGCTGCAGGAGCCGCCGCAGCCGGAGCCAAAGTTCCTCTAGG
TCCCGAAGTCGTGGCAGGAGGAGGTCGGGTAATAGAGACAGAACTGTTCAGAGTCCCAAA
TCCAAA


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pENTR223

pENTR223 in advanced viewed

Synonyms: pDONR223, pENTR223-XnCDSYn
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp): 2815
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Mark Vidal
Publications: PMID: 15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 2220 2753
gene fragment pas 5p stem of pBR primose assembly site 63 73
gene fragment pas prime 3p stem of pBR pas(L) 105 116
recombination site attL1 attL1 recombination site 570 665
recombination site attL2 attL2 recombination site 793 696
selectable marker SpnR spectinomycin resistance gene 1062 2072
trxn termination sequence T1-T2 rrnBT1 and T2 terminators 510 224

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ZRSR2
Symbol Nomenclature : ZRSR2
SYNONYM : U2AF1-RS2; U2AF1L2; U2AF1RS2; URP
Designation : U2 small nuclear ribonucleoprotein auxiliary factor 35 kDa subunit-related protein 2|U2 small nuclear ribonucleoprotein auxiliary factor, small subunit 2|U2(RNU2) small nuclear RNA auxiliary factor 1-like 2|U2AF35-related protein|renal carcinoma antigen NY-REN-20
Full Nomenclature : zinc finger (CCCH type), RNA-binding motif and serine/arginine rich 2
GENEID : 8233
GenBank Accession : HQ258575
HGNC : 23019
MIM : 300028
Vega : OTTHUMG00000021184
Target GenBank: HQ258575

 

Pathways

Panther : mRNA splicing

Protein

SMART domain : RRM_1 : RNA recognition motif
SMART domain : RRM : RNA recognition motif
SMART domain : ZnF_C3H1 : zinc finger
HPRD : 02068

Clone

Cloning Information : 611606
Original Clone ID : ccsbBroadEn_07211

Organism


TAX_ID : 9606
Species Specific ID: 8233

Genome

Chromosome : X
Map Location : Xp22.1
Ensembl : ENSG00000169249

Reagents

Labome : ZRSR2-antibody