DNASU Plasmid | LILRA1 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: LILRA1

Gene Name: leukocyte immunoglobulin-like receptor, subfamily A (with TM domain), member 1

Original Clone ID:

ccsbBroadEn_02599

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC093708.1

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00515838

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1468nts

Open reading frame: 1 to 1468

Reference Sequence Alignment

ATGACCCCCATCGTCACAGTCCTGATCTGTCTCAGGCTGAGTCTGGGCCCCCGGACCCAC
GTGCAGGCAGGGACCCTCCCCAAGCCCACACTCTGGGCTGAGCCAGGCTCTGTGATCACC
CAGGGGAGTCCCGTGACCCTCTGGTGTCAGGGGATCCTGGAGACCCAGGAGTACCGTCTG
TATAGAGAAAAGAAAACAGCACCCTGGATTACACGGATCCCACAGGAGATTGTGAAGAAG
GGCCAGTTCCCCATCCCATCCATCACCTGGGAACACACAGGGCGGTATCGCTGTTTCTAC
GGTAGCCACACTGCAGGCTGGTCAGAGCCCAGTGACCCCCTGGAGCTGGTGGTGACAGGA
GCCTACATCAAACCCACCCTCTCAGCTCTACCCAGCCCTGTGGTGACCTCAGGAGGGAAC
GTGACCCTCCATTGTGTCTCACAGGTGGCATTTGGCAGCTTCATTCTGTGTAAGGAAGGA
GAAGATGAACACCCACAATGCCTGAACTCACAGCCCCGTACCCATGGGTGGTCCCGGGCC
ATCTTCTCTGTGGGCCCCGTGAGCCCGAGTCGCAGGTGGTCGTACAGGTGCTATGCTTAT
GACTCGAACTCTCCCCATGTGTGGTCTCTACCCAGTGATCTCCTGGAGCTCCTGGTCCTA
GGTGTTTCTAAGAAGCCATCACTCTCAGTGCAGCCAGGTCCTATAGTGGCCCCTGGGGAG
AGCCTGACCCTCCAGTGTGTTTCTGATGTCAGCTACGACAGATTTGTTCTGTATAAGGAG
GGAGAACGTGACTTCCTCCAGCTCCCTGGCCCACAGCCCCAGGCTGGGCTCTCCCAGGCC
AACTTCACCCTGGGCCCTGTGAGCCGCTCCTACGGGGGCCAGTACAGATGCTCCGGTGCA
TACAACCTCTCCTCCGAGTGGTCGGCCCCCAGCGACCCCCTGGACATCCTGATCGCAGGA
CAGTTCCGTGGCAGACCCTTCATCTCGGTGCATCCGGGCCCCACGGTGGCCTCAGGAGAG
AACGTGACCCTGCTGTGTCAGTCATGGGGGCCGTTCCACACTTTCCTTCTGACCAAGGCG
GGAGCAGCTGATGCCCCCCTCCGTCTCAGATCAATACACGAATATCCTAAGTACCAGGCT
GAATTCCCTATGAGTCCTGTGACCTCAGCCCACTCGGGGACCTACAGGTGCTACGGCTCA
CTCAGCTCCAACCCCTACCTGCTGTCTCACCCCAGTGACTCCCTGGAGCTCATGGTCTCA
GGAGCAGCTGAGACCCTCAGCCCACCACAAAACAAGTCCGATTCCAAGGCTGGAGCAGCT
AACACCCTCAGCCCATCACAAAACAAGACTGCCTCACACCCCCAGGATTACACAGTGGAG
AATCTCATCCGCATGGGCATAGCTGGCTTGGTCCTGGTGGTCCTCGGGATTCTGCTATTT
GAGGCTCAGCACAGCCAGAGAAGCCTC

Protein sequence predicted based on nucleotide sequence

MTPIVTVLICLRLSLGPRTHVQAGTLPKPTLWAEPGSVITQGSPVTLWCQGILETQEYRL
YREKKTAPWITRIPQEIVKKGQFPIPSITWEHTGRYRCFYGSHTAGWSEPSDPLELVVTG
AYIKPTLSALPSPVVTSGGNVTLHCVSQVAFGSFILCKEGEDEHPQCLNSQPRTHGWSRA
IFSVGPVSPSRRWSYRCYAYDSNSPHVWSLPSDLLELLVLGVSKKPSLSVQPGPIVAPGE
SLTLQCVSDVSYDRFVLYKEGERDFLQLPGPQPQAGLSQANFTLGPVSRSYGGQYRCSGA
YNLSSEWSAPSDPLDILIAGQFRGRPFISVHPGPTVASGENVTLLCQSWGPFHTFLLTKA
GAADAPLRLRSIHEYPKYQAEFPMSPVTSAHSGTYRCYGSLSSNPYLLSHPSDSLELMVS
GAAETLSPPQNKSDSKAGAANTLSPSQNKTASHPQDYTVENLIRMGIAGLVLVVLGILLF
EAQHSQRSL

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

LILRA1

Symbol Nomenclature :

LILRA1

SYNONYM :

CD85I; LIR-6; LIR6

Designation :

CD85 antigen-like family member I|leukocyte immunoglobulin-like receptor 6|leukocyte immunoglobulin-like receptor subfamily A member 1

Full Nomenclature :

leukocyte immunoglobulin-like receptor, subfamily A (with TM domain), member 1

GENEID :

11024

Locus Tag :

XXbac-BCX85G21.3

HGNC :

6602

MIM :

604810

Vega :

OTTHUMG00000065701

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00515838      ATGACCCCCATCGTCACAGTCCTGATCTGTCTCAGGCTGAGTCTGGGCCCCCGGACCCAC
NM_006863.3       ATGACCCCCATCGTCACAGTCCTGATCTGTCTCAGGCTGAGTCTGGGCCCCCGGACCCAC
                  ************************************************************

HsCD00515838      GTGCAGGCAGGGACCCTCCCCAAGCCCACACTCTGGGCTGAGCCAGGCTCTGTGATCACC
NM_006863.3       GTGCAGGCAGGGACCCTCCCCAAGCCCACACTCTGGGCTGAGCCAGGCTCTGTGATCACC
                  ************************************************************

HsCD00515838      CAGGGGAGTCCCGTGACCCTCTGGTGTCAGGGGATCCTGGAGACCCAGGAGTACCGTCTG
NM_006863.3       CAGGGGAGTCCCGTGACCCTCTGGTGTCAGGGGATCCTGGAGACCCAGGAGTACCGTCTG
                  ************************************************************

HsCD00515838      TATAGAGAAAAGAAAACAGCACCCTGGATTACACGGATCCCACAGGAGATTGTGAAGAAG
NM_006863.3       TATAGAGAAAAGAAAACAGCACCCTGGATTACACGGATCCCACAGGAGATTGTGAAGAAG
                  ************************************************************

HsCD00515838      GGCCAGTTCCCCATCCCATCCATCACCTGGGAACACACAGGGCGGTATCGCTGTTTCTAC
NM_006863.3       GGCCAGTTCCCCATCCCATCCATCACCTGGGAACACACAGGGCGGTATCGCTGTTTCTAC
                  ************************************************************

HsCD00515838      GGTAGCCACACTGCAGGCTGGTCAGAGCCCAGTGACCCCCTGGAGCTGGTGGTGACAGGA
NM_006863.3       GGTAGCCACACTGCAGGCTGGTCAGAGCCCAGTGACCCCCTGGAGCTGGTGGTGACAGGA
                  ************************************************************

HsCD00515838      GCCTACATCAAACCCACCCTCTCAGCTCTACCCAGCCCTGTGGTGACCTCAGGAGGGAAC
NM_006863.3       GCCTACATCAAACCCACCCTCTCAGCTCTACCCAGCCCTGTGGTGACCTCAGGAGGGAAC
                  ************************************************************

HsCD00515838      GTGACCCTCCATTGTGTCTCACAGGTGGCATTTGGCAGCTTCATTCTGTGTAAGGAAGGA
NM_006863.3       GTGACCCTCCATTGTGTCTCACAGGTGGCATTTGGCAGCTTCATTCTGTGTAAGGAAGGA
                  ************************************************************

HsCD00515838      GAAGATGAACACCCACAATGCCTGAACTCACAGCCCCGTACCCATGGGTGGTCCCGGGCC
NM_006863.3       GAAGATGAACACCCACAATGCCTGAACTCACAGCCCCGTACCCATGGGTGGTCCCGGGCC
                  ************************************************************

HsCD00515838      ATCTTCTCTGTGGGCCCCGTGAGCCCGAGTCGCAGGTGGTCGTACAGGTGCTATGCTTAT
NM_006863.3       ATCTTCTCTGTGGGCCCCGTGAGCCCGAGTCGCAGGTGGTCGTACAGGTGCTATGCTTAT
                  ************************************************************

HsCD00515838      GACTCGAACTCTCCCCATGTGTGGTCTCTACCCAGTGATCTCCTGGAGCTCCTGGTCCTA
NM_006863.3       GACTCGAACTCTCCCCATGTGTGGTCTCTACCCAGTGATCTCCTGGAGCTCCTGGTCCTA
                  ************************************************************

HsCD00515838      GGTGTTTCTAAGAAGCCATCACTCTCAGTGCAGCCAGGTCCTATAGTGGCCCCTGGGGAG
NM_006863.3       GGTGTTTCTAAGAAGCCATCACTCTCAGTGCAGCCAGGTCCTATAGTGGCCCCTGGGGAG
                  ************************************************************

HsCD00515838      AGCCTGACCCTCCAGTGTGTTTCTGATGTCAGCTACGACAGATTTGTTCTGTATAAGGAG
NM_006863.3       AGCCTGACCCTCCAGTGTGTTTCTGATGTCAGCTACGACAGATTTGTTCTGTATAAGGAG
                  ************************************************************

HsCD00515838      GGAGAACGTGACTTCCTCCAGCTCCCTGGCCCACAGCCCCAGGCTGGGCTCTCCCAGGCC
NM_006863.3       GGAGAACGTGACTTCCTCCAGCTCCCTGGCCCACAGCCCCAGGCTGGGCTCTCCCAGGCC
                  ************************************************************

HsCD00515838      AACTTCACCCTGGGCCCTGTGAGCCGCTCCTACGGGGGCCAGTACAGATGCTCCGGTGCA
NM_006863.3       AACTTCACCCTGGGCCCTGTGAGCCGCTCCTACGGGGGCCAGTACAGATGCTCCGGTGCA
                  ************************************************************

HsCD00515838      TACAACCTCTCCTCCGAGTGGTCGGCCCCCAGCGACCCCCTGGACATCCTGATCGCAGGA
NM_006863.3       TACAACCTCTCCTCCGAGTGGTCGGCCCCCAGCGACCCCCTGGACATCCTGATCGCAGGA
                  ************************************************************

HsCD00515838      CAGTTCCGTGGCAGACCCTTCATCTCGGTGCATCCGGGCCCCACGGTGGCCTCAGGAGAG
NM_006863.3       CAGTTCCGTGGCAGACCCTTCATCTCGGTGCATCCGGGCCCCACGGTGGCCTCAGGAGAG
                  ************************************************************

HsCD00515838      AACGTGACCCTGCTGTGTCAGTCATGGGGGCCGTTCCACACTTTCCTTCTGACCAAGGCG
NM_006863.3       AACGTGACCCTGCTGTGTCAGTCATGGGGGCCGTTCCACACTTTCCTTCTGACCAAGGCG
                  ************************************************************

HsCD00515838      GGAGCAGCTGATGCCCCCCTCCGTCTCAGATCAATACACGAATATCCTAAGTACCAGGCT
NM_006863.3       GGAGCAGCTGATGCCCCCCTCCGTCTCAGATCAATACACGAATATCCTAAGTACCAGGCT
                  ************************************************************

HsCD00515838      GAATTCCCTATGAGTCCTGTGACCTCAGCCCACTCGGGGACCTACAGGTGCTACGGCTCA
NM_006863.3       GAATTCCCTATGAGTCCTGTGACCTCAGCCCACTCGGGGACCTACAGGTGCTACGGCTCA
                  ************************************************************

HsCD00515838      CTCAGCTCCAACCCCTACCTGCTGTCTCACCCCAGTGACTCCCTGGAGCTCATGGTCTCA
NM_006863.3       CTCAGCTCCAACCCCTACCTGCTGTCTCACCCCAGTGACTCCCTGGAGCTCATGGTCTCA
                  ************************************************************

HsCD00515838      GGAGCAGCTGAGACCCTCAGCCCACCACAAAACAAGTCCGATTCCAAGGCTGGAGCAGCT
NM_006863.3       GGAGCAGCTGAGACCCTCAGCCCACCACAAAACAAGTCCGATTCCAAGGCTGGAGCAGCT
                  ************************************************************

HsCD00515838      AACACCCTCAGCCCATCACAAAACAAGACTGCCTCACACCCCCAGGATTACACAGTGGAG
NM_006863.3       AACACCCTCAGCCCATCACAAAACAAGACTGCCTCACACCCCCAGGATTACACAGTGGAG
                  ************************************************************

HsCD00515838      AATCTCATCCGCATGGGCATAGCTGGCTTGGTCCTGGTGGTCCTCGGGATTCTGCTATTT
NM_006863.3       AATCTCATCCGCATGGGCATAGCTGGCTTGGTCCTGGTGGTCCTCGGGATTCTGCTATTT
                  ************************************************************

HsCD00515838      GAGGCTCAGCACAGCCAGAGAAGCCTC---
NM_006863.3       GAGGCTCAGCACAGCCAGAGAAGCCTCTGA
                  ***************************