Gene:
Gene Symbol:
PDIA4
Gene Name:
protein disulfide isomerase family A, member 4
Original Clone ID:
None
Keyword:
partial cds (C-terminal deletion)
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pGEc2-DEST
Format:
FUSION
Source:
Glyco-enzyme clone collection
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
University of Georgia
Center for Personalized Diagnostics
Arizona State University
Kelley Moremen
Center for Personalized Diagnostics
Arizona State University
Kelley Moremen
HsCD00521206
Price:
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AVAILABLE
No restriction
Special MTA: SPTA
Special MTA: SPTA
ATGAGGCCCCGGAAAGCCTTCCTGCTCCTGCTGCTCTTGGGGCTGGTGCAGCTGCTGGCC
GTGGCGGGTGCCGAGGGCCCGGACGAGGATTCTTCTAACAGAGAAAATGCCATTGAGGAT
GAAGAGGAGGAGGAGGAGGAAGATGATGATGAGGAAGAAGACGACTTGGAAGTTAAGGAA
GAAAATGGAGTCTTGGTCCTAAATGATGCAAACTTTGATAATTTTGTGGCTGACAAAGAC
ACAGTGCTGCTGGAGTTTTATGCTCCATGGTGTGGACATTGCAAGCAGTTTGCTCCGGAA
TATGAAAAAATTGCCAACATATTAAAGGATAAAGATCCTCCCATTCCTGTTGCCAAGATC
GATGCAACCTCAGCGTCTGTGCTGGCCAGCAGGTTTGATGTGAGTGGCTACCCCACCATC
AAGATCCTTAAGAAGGGGCAGGCTGTAGACTACGAGGGCTCCAGAACCCAGGAAGAAATT
GTTGCCAAGGTCAGAGAAGTCTCCCAGCCCGACTGGACGCCTCCACCAGAAGTCACGCTT
GTGTTGACCAAAGAGAACTTTGATGAAGTTGTGAATGATGCAGATATCATTCTGGTGGAG
TTTTATGCCCCATGGTGTGGACACTGCAAGAAACTTGCCCCCGAGTATGAGAAGGCCGCC
AAGGAGCTCAGCAAGCGTTCTCCTCCAATTCCCCTGGCAAAGGTCGACGCCACCGCAGAA
ACAGACCTGGCCAAGAGGTTTGATGTCTCTGGCTATCCCACCCTGAAAATTTTCCGCAAA
GGAAGGCCTTATGACTACAACGGCCCACGAGAAAAATATGGAATCGTTGATTACATGATC
GAGCAGTCCGGGCCTCCCTCCAAGGAGATTCTGACCCTGAAGCAGGTCCAGGAGTTCCTG
AAGGATGGAGACGATGTCATCATCATCGGGGTCTTTAAGGGGGAGAGTGACCCAGCCTAC
CAGCAATACCAGGATGCCGCTAACAACCTGAGAGAAGATTACAAATTTCACCACACTTTC
AGCACAGAAATAGCAAAGTTCTTGAAAGTCTCCCAGGGGCAGTTGGTTGTAATGCAGCCT
GAGAAATTCCAGTCCAAGTATGAGCCCCGGAGCCACATGATGGACGTCCAGGGCTCCACC
CAGGACTCGGCCATCAAGGACTTCGTGCTGAAGTACGCCCTGCCCCTGGTTGGCCACCGC
AAGGTGTCAAACGATGCTAAGCGCTACACCAGGCGCCCCCTGGTGGTCGTCTACTACAGT
GTGGACTTCAGCTTTGATTACAGAGCTGCAACTCAGTTTTGGCGGAGCAAAGTCCTAGAG
GTGGCCAAGGACTTCCCTGAGTACACCTTTGCCATTGCGGACGAAGAGGACTATGCTGGG
GAGGTGAAGGACCTGGGGCTCAGCGAGAGTGGGGAGGATGTCAATGCCGCCATCCTGGAC
GAGAGTGGGAAGAAGTTCGCCATGGAGCCAGAGGAGTTTGACTCTGACACCCTCCGCGAG
TTTGTCACTGCTTTCAAAAAAGGAAAACTGAAGCCAGTCATCAAATCCCAGCCAGTGCCC
AAGAACAACAAGGGACCCGTCAAGGTCGTGGTGGGAAAGACCTTTGACTCCATTGTGATG
GACCCCAAGAAGGACGTCCTCATCGAGTTCTACGCACCATGGTGCGGGCACTGCAAGCAG
CTAGAGCCCGTGTACAACAGCCTGGCCAAGAAGTACAAGGGCCAAAAGGGCCTGGTCATC
GCCAAGATGGACGCCACTGCCAACGACGTCCCCAGCGACCGCTATAAGGTGGAGGGCTTC
CCCACCATCTACTTCGCCCCCAGTGGGGACAAAAAGAACCCAGTTAAATTTGAGGGTGGA
GACAGAGATCTGGAGCATTTGAGCAAGTTTATAGAAGAACATGCCACAAAACTGAGCAGG
ACC
Protein sequence predicted based on nucleotide sequence
MRPRKAFLLLLLLGLVQLLAVAGAEGPDEDSSNRENAIEDEEEEEEEDDDEEEDDLEVKE ENGVLVLNDANFDNFVADKDTVLLEFYAPWCGHCKQFAPEYEKIANILKDKDPPIPVAKI DATSASVLASRFDVSGYPTIKILKKGQAVDYEGSRTQEEIVAKVREVSQPDWTPPPEVTL VLTKENFDEVVNDADIILVEFYAPWCGHCKKLAPEYEKAAKELSKRSPPIPLAKVDATAE TDLAKRFDVSGYPTLKIFRKGRPYDYNGPREKYGIVDYMIEQSGPPSKEILTLKQVQEFL KDGDDVIIIGVFKGESDPAYQQYQDAANNLREDYKFHHTFSTEIAKFLKVSQGQLVVMQP EKFQSKYEPRSHMMDVQGSTQDSAIKDFVLKYALPLVGHRKVSNDAKRYTRRPLVVVYYS VDFSFDYRAATQFWRSKVLEVAKDFPEYTFAIADEEDYAGEVKDLGLSESGEDVNAAILD ESGKKFAMEPEEFDSDTLREFVTAFKKGKLKPVIKSQPVPKNNKGPVKVVVGKTFDSIVM DPKKDVLIEFYAPWCGHCKQLEPVYNSLAKKYKGQKGLVIAKMDATANDVPSDRYKVEGF PTIYFAPSGDKKNPVKFEGGDRDLEHLSKFIEEHATKLSRT
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pGEc2-DEST
Synonyms:
''
Sequencing Primer:
Forward:
pGEc1-DEST F
Reverse:
pGEn1-DEST R
Description:
Mammalian expression vector with a CMV promoter and C-terminal Superfolder GFP, AviTag and 10xHis tag; ampicillin resistance in bacteria; Gateway recombinational cloning
Comments:
None
Size (bp):
6852
Parent Vector:
Name:
pXKM
Description:
Properties:
Gateway, acceptor (destination), fluorescent marker, mammalian transduction, recombinational cloning, with tag/fusion/marker
Author Name:
University of Georgia
Kelley Moremen
Kelley Moremen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
1137
1804
death cassette
ccdB
ccdB death cassette (removed in plasmids with insert)
4211
4516
intron
artificial intron
artificial intron
2828
2960
poly-A signal
BGH pA
bovine growth hormone (BGH) polyadenylation signal
6028
6242
promoter
CMV
CMV promoter for expression in mammalian cells
2208
2790
recombination site
3'-ITR
inverted terminal repeat sequence
6330
6459
recombination site
attR1
attR1 recombinational cloning site
2974
3101
recombination site
attR2
attR2 recombinational cloning site
4557
4681
selectable marker
AmpR
ampicillin resistance
129
989
selectable marker
CmR
Chloramphenicol resistance (CmR) gene (removed in plasmids with insert)
3210
3859
tag
GFP
C-terminal super GFP
4682
5377
tag
AviTag
C-terminal AviTag
5378
5428
tag
His
C-terminal 10xHis tag
5429
5452
trxn regulatory element
WPRE
woodchuck hepatitis post-transcriptional regulatory element
5480
6021
