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FGF12 (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  FGF12
Gene Name:  fibroblast growth factor 12
Sequence              Map: pDNR-Dual_with_human_insert.pdf
Original Clone ID: FLH139886.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDNR-Dual               Format:  CLOSED
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: PMID: 16512675
Title: Functional proteomics approach to investigate the biological activities of cDNAs implicated in breast cancer.
Authors: HIP
HsCD00005241

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 546nts         Open reading frame : 1 to 546
Reference Sequence Alignment

ATGGAGAGCAAAGAACCCCAGCTAAAAGGGATTGTGACAAGGTTATTCAGCCAGCAGGGA
TACTTCCTGCAGATGCACCCAGATGGTACCATTGATGGGACCAAGGACGAAAACAGCGAC
TACACTCTCTTCAATCTAATTCCCGTGGGCCTGCGTGTAGTGGCCATCCAAGGAGTGAAG
GCTAGCCTCTATGTGGCCATGAATGGTGAAGGCTATCTCTACAGTTCAGATGTTTTCACT
CCAGAATGCAAATTCAAGGAATCTGTGTTTGAAAACTACTATGTGATCTATTCTTCCACA
CTGTACCGCCAGCAAGAATCAGGCCGAGCTTGGTTTCTGGGACTCAATAAAGAAGGTCAA
ATTATGAAGGGGAACAGAGTGAAGAAAACCAAGCCCTCATCACATTTTGTACCGAAACCT
ATTGAAGTGTGTATGTACAGAGAACAATCGCTACATGAAATTGGAGAAAAACAAGGGCGT
TCAAGGAAAAGTTCTGGAACACCAACCATGAATGGAGGCAAAGTTGTGAATCAAGATTCA
ACATAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 776
Start on reference sequence 231
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDNR-Dual

pDNR-Dual in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  Unknown
Description: Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from).
Comments: The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp): 4938
Parent Vector: None
Empty Vector: None
Properties: Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name: BD Biosciences Clontech
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS restriction enzyme-type multiple cloning site 45 129
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4111 4574
negative selection marker SacB SacB negative selection marker gene (sucrose sensitivity) 1057 2972
primer site M13 primer M13 primer sequencing site 4839 4855
primer site T7 primer T7 RNA Pol primer sequencing site 4865 4883
recombination site loxP site loxP recombination site 1015 1048
recombination site loxP site loxP recombination site 9 42
selectable marker CmR chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) 986 327
selectable marker AmpR ampicillin resistance gene 3103 3963
splice donor site SD site splice donor site 132 136
tag HN 6xHN epitope tag 141 176

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : FGF12
Symbol Nomenclature : FGF12
SYNONYM : FGF12B; FHF1
Designation : FGF-12|FHF-1|fibroblast growth factor 12B|fibroblast growth factor FGF-12b|fibroblast growth factor homologous factor 1|myocyte-activating factor
Full Nomenclature : fibroblast growth factor 12
GENEID : 2257
GI : 61363740
GenBank Accession : AY890495
HGNC : 3668
MIM : 601513
Vega : OTTHUMG00000156132
Target GenBank: BC022524

 

Reference Sequence Alignment

  

HsCD00005241        ------------------------------------------------------------
NM_004113.5         ------------------------------------------------------------
XM_005247227.1      --------------ATGAAGATTCTTGAGCTTTCTCTGAAGAAAGGAGTGCATATGCTGC
NM_021032.4         ATGGCTGCGGCGATAGCCAGCTCCTTGATCCGGCAGAAGCGGCAGGCGAGGGAGTCCAAC
                                                                                

HsCD00005241        ------------------------------------------------------------
NM_004113.5         ------------------------------------------------------------
XM_005247227.1      AGTGTCTCTGTGGAAGGAGCCTG-------------------------------------
NM_021032.4         AGCGACCGAGTGTCGGCCTCCAAGCGCCGCTCCAGCCCCAGCAAAGACGGGCGCTCCCTG
                                                                                

HsCD00005241        ------------------------------------------------------------
NM_004113.5         ------------------------------------------------------------
XM_005247227.1      ---------------------------------------------------------AGG
NM_021032.4         TGCGAGAGGCACGTCCTCGGGGTGTTCAGCAAAGTGCGCTTCTGCAGCGGCCGCAAGAGG
                                                                                

HsCD00005241        ------ATGGAGAGCAAAGAACCCCAGCTAAAAGGGATTGTGACAAGGTTATTCAGCCAG
NM_004113.5         ------ATGGAGAGCAAAGAACCCCAGCTCAAAGGGATTGTGACAAGGTTATTCAGCCAG
XM_005247227.1      TCCAGCGACAGCCCAAGTGAACCCCAGCTCAAAGGGATTGTGACAAGGTTATTCAGCCAG
NM_021032.4         CCGGTGAGGCGGAGACCAGAACCCCAGCTCAAAGGGATTGTGACAAGGTTATTCAGCCAG
                          .   . . .. :***********.******************************

HsCD00005241        CAGGGATACTTCCTGCAGATGCACCCAGATGGTACCATTGATGGGACCAAGGACGAAAAC
NM_004113.5         CAGGGATACTTCCTGCAGATGCACCCAGATGGTACCATTGATGGGACCAAGGACGAAAAC
XM_005247227.1      CAGGGATACTTCCTGCAGATGCACCCAGATGGTACCATTGATGGGACCAAGGACGAAAAC
NM_021032.4         CAGGGATACTTCCTGCAGATGCACCCAGATGGTACCATTGATGGGACCAAGGACGAAAAC
                    ************************************************************

HsCD00005241        AGCGACTACACTCTCTTCAATCTAATTCCCGTGGGCCTGCGTGTAGTGGCCATCCAAGGA
NM_004113.5         AGCGACTACACTCTCTTCAATCTAATTCCCGTGGGCCTGCGTGTAGTGGCCATCCAAGGA
XM_005247227.1      AGCGACTACACTCTCTTCAATCTAATTCCCGTGGGCCTGCGTGTAGTGGCCATCCAAGGA
NM_021032.4         AGCGACTACACTCTCTTCAATCTAATTCCCGTGGGCCTGCGTGTAGTGGCCATCCAAGGA
                    ************************************************************

HsCD00005241        GTGAAGGCTAGCCTCTATGTGGCCATGAATGGTGAAGGCTATCTCTACAGTTCAGATGTT
NM_004113.5         GTGAAGGCTAGCCTCTATGTGGCCATGAATGGTGAAGGCTATCTCTACAGTTCAGATGTT
XM_005247227.1      GTGAAGGCTAGCCTCTATGTGGCCATGAATGGTGAAGGCTATCTCTACAGTTCAGATGTT
NM_021032.4         GTGAAGGCTAGCCTCTATGTGGCCATGAATGGTGAAGGCTATCTCTACAGTTCAGATGTT
                    ************************************************************

HsCD00005241        TTCACTCCAGAATGCAAATTCAAGGAATCTGTGTTTGAAAACTACTATGTGATCTATTCT
NM_004113.5         TTCACTCCAGAATGCAAATTCAAGGAATCTGTGTTTGAAAACTACTATGTGATCTATTCT
XM_005247227.1      TTCACTCCAGAATGCAAATTCAAGGAATCTGTGTTTGAAAACTACTATGTGATCTATTCT
NM_021032.4         TTCACTCCAGAATGCAAATTCAAGGAATCTGTGTTTGAAAACTACTATGTGATCTATTCT
                    ************************************************************

HsCD00005241        TCCACACTGTACCGCCAGCAAGAATCAGGCCGAGCTTGGTTTCTGGGACTCAATAAAGAA
NM_004113.5         TCCACACTGTACCGCCAGCAAGAATCAGGCCGAGCTTGGTTTCTGGGACTCAATAAAGAA
XM_005247227.1      TCCACACTGTACCGCCAGCAAGAATCAGGCCGAGCTTGGTTTCTGGGACTCAATAAAGAA
NM_021032.4         TCCACACTGTACCGCCAGCAAGAATCAGGCCGAGCTTGGTTTCTGGGACTCAATAAAGAA
                    ************************************************************

HsCD00005241        GGTCAAATTATGAAGGGGAACAGAGTGAAGAAAACCAAGCCCTCATCACATTTTGTACCG
NM_004113.5         GGTCAAATTATGAAGGGGAACAGAGTGAAGAAAACCAAGCCCTCATCACATTTTGTACCG
XM_005247227.1      GGTCAAATTATGAAGGGGAACAGAGTGAAGAAAACCAAGCCCTCATCACATTTTGTACCG
NM_021032.4         GGTCAAATTATGAAGGGGAACAGAGTGAAGAAAACCAAGCCCTCATCACATTTTGTACCG
                    ************************************************************

HsCD00005241        AAACCTATTGAAGTGTGTATGTACAGAGAACAATCGCTACATGAAATTGGAGAAAAACAA
NM_004113.5         AAACCTATTGAAGTGTGTATGTACAGAGAACCATCGCTACATGAAATTGGAGAAAAACAA
XM_005247227.1      AAACCTATTGAAGTGTGTATGTACAGAGAACCATCGCTACATGAAATTGGAGAAAAACAA
NM_021032.4         AAACCTATTGAAGTGTGTATGTACAGAGAACCATCGCTACATGAAATTGGAGAAAAACAA
                    *******************************.****************************

HsCD00005241        GGGCGTTCAAGGAAAAGTTCTGGAACACCAACCATGAATGGAGGCAAAGTTGTGAATCAA
NM_004113.5         GGGCGTTCAAGGAAAAGTTCTGGAACACCAACCATGAATGGAGGCAAAGTTGTGAATCAA
XM_005247227.1      GGGCGTTCAAGGAAAAGTTCTGGAACACCAACCATGAATGGAGGCAAAGTTGTGAATCAA
NM_021032.4         GGGCGTTCAAGGAAAAGTTCTGGAACACCAACCATGAATGGAGGCAAAGTTGTGAATCAA
                    ************************************************************

HsCD00005241        GATTCAACATAG
NM_004113.5         GATTCAACATAG
XM_005247227.1      GATTCAACATAG
NM_021032.4         GATTCAACATAG
                    ************

Pathways

Panther : FGF signaling pathway
WikiPathway : Regulation of Actin Cytoskeleton

Protein

SMART domain : FGF : Acidic and basic fibroblast growth factor family.
UniProt : P61328
HPRD : 03303

Clone

Cloning Information : 139886
HIP Master Clone ID : 116153
Original Clone ID : FLH139886.01X

Organism


TAX_ID : 9606
Species Specific ID: 2257

Genome

Chromosome : 3
Map Location : 3q28
Ensembl : ENSG00000114279

Reagents

Labome : FGF12-antibody