Gene:
Gene Symbol:
TM1246
Gene Name:
phosphoribosylformylglycinamidine synthase II
Original Clone ID:
7728
Keyword:
full-length cds
Species:
Thermotoga maritima
Type:
cDNA
Vector Name:
pMH2T7
Format:
CLOSED
Source:
Joint Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
PMID: 16544324
Title: Crystal structure of phosphoribosylformylglycinamidine synthase II (smPurL) from Thermotoga maritima at 2.15 A resolution.
Title: Crystal structure of phosphoribosylformylglycinamidine synthase II (smPurL) from Thermotoga maritima at 2.15 A resolution.
Authors:
PSI
Joint Center for Structural Genomics
Joint Center for Structural Genomics
TmCD00530440
Price:
Login for Pricing
AVAILABLE
No restriction
Special MTA: PSI
Special MTA: PSI
Insert sequence: 1812nts
Open reading frame: 1 to 1812
ATGAAGTTGAGATATCTGAACATTCTCAAGGAGAAGCTTGGAAGAGAACCCACCTTCGTC
GAGCTTCAGGCTTTTTCAGTCATGTGGAGCGAACACTGTGGATACTCCCACACGAAGAAA
TACATAAGAAGATTACCGAAAACGGGTTTTGAGGGAAACGCGGGTGTGGTGAACCTGGAT
GATTACTATTCCGTTGCTTTCAAGATAGAGAGCCACAATCACCCGAGTGCGATAGAACCT
TACAACGGTGCAGCAACGGGTGTCGGTGGAATCATCAGAGACGTCCTCGCGATGGGAGCC
CGCCCAACGGCCATATTCGATTCACTCCACATGTCCCGAATCATAGACGGTATCATAGAG
GGAATAGCCGATTACGGTAATTCCATAGGCGTTCCAACGGTCGGTGGAGAACTCAGGATC
TCCTCACTATACGCGCACAACCCCCTCGTGAACGTGCTCGCAGCGGGTGTTGTGAGAAAC
GACATGCTGGTCGATTCGAAAGCATCGAGACCGGGACAGGTCATAGTGATCTTCGGAGGA
GCCACAGGAAGGGACGGAATCCACGGGGCGTCTTTCGCTTCGGAGGATCTCACGGGTGAT
AAGGCAACCAAGCTTTCAATTCAGGTTGGTGACCCGTTCGCTGAAAAGATGCTCATAGAA
GCGTTCCTGGAGATGGTGGAAGAGGGACTCGTGGAAGGAGCTCAGGATCTGGGAGCGGGC
GGTGTGCTGTCCGCAACTTCGGAACTCGTAGCGAAGGGGAATCTCGGAGCGATCGTTCAT
CTGGACCGTGTTCCCCTGAGAGAACCGGATATGGAGCCCTGGGAAATCCTCATAAGCGAA
AGCCAAGAAAGAATGGCGGTCGTCACATCACCTCAGAAGGCGAGCCGCATTCTGGAAATA
GCCAGGAAGCACCTTCTTTTCGGCGATGTCGTTGCCGAAGTGATAGAAGAACCAGTTTAC
AGGGTGATGTACAGAAACGATCTCGTCATGGAAGTTCCCGTCCAGCTCCTCGCAAACGCC
CCAGAAGAGGACATAGTCGAATACACACCCGGAAAGATCCCAGAGTTCAAAAGGGTGGAA
TTCGAAGAGGTGAACGCGAGGGAAGTCTTCGAGCAGTACGATCACATGGTCGGAACGGAT
ACCGTGGTTCCACCCGGATTCGGCGCGGCCGTGATGAGAATAAAGAGAGACGGGGGGTAC
TCCCTCGTCACACACAGCAGGGCGGATCTGGCTCTTCAGGATACCTACTGGGGAACCCTC
ATAGCCGTGCTCGAGAGTGTGAGAAAGACGCTCAGCGTTGGTGCGGAACCCCTCGCTATA
ACGAACTGCGTGAACTACGGGGATCCCGATGTTGATCCGGTGGGACTTTCGGCCATGATG
ACCGCTCTCAAGAACGCTTGCGAATTCTCCGGTGTTCCCGTTGCATCGGGAAACGCTTCT
CTCTACAACACGTACCAGGGAAAACCCATTCCACCCACCCTGGTTGTGGGAATGTTGGGG
AAGGTGAACCCGCAGAAAGTGGCAAAACCGAAGCCTTCGAAGGTGTTCGCGGTGGGATGG
AACGACTTCGAACTCGAAAGGGAAAAAGAACTCTGGAGAGCCATAAGAAAACTCTCAGAA
GAAGGTGCTTTCATCCTCTCTTCGTCACAGCTTCTGACGAGAACTCACGTGGAGACTTTC
AGAGAGTACGGCTTGAAGATCGAAGTGAAACTGCCAGAAGTGAGGCCGGCGCATCAGATG
GTGCTCGTCTTCTCTGAGAGAACTCCCGTAGTCGATGTTCCTGTGAAGGAGATCGGAACG
CTCTCGAGGTAA
Protein sequence predicted based on nucleotide sequence
MKLRYLNILKEKLGREPTFVELQAFSVMWSEHCGYSHTKKYIRRLPKTGFEGNAGVVNLD DYYSVAFKIESHNHPSAIEPYNGAATGVGGIIRDVLAMGARPTAIFDSLHMSRIIDGIIE GIADYGNSIGVPTVGGELRISSLYAHNPLVNVLAAGVVRNDMLVDSKASRPGQVIVIFGG ATGRDGIHGASFASEDLTGDKATKLSIQVGDPFAEKMLIEAFLEMVEEGLVEGAQDLGAG GVLSATSELVAKGNLGAIVHLDRVPLREPDMEPWEILISESQERMAVVTSPQKASRILEI ARKHLLFGDVVAEVIEEPVYRVMYRNDLVMEVPVQLLANAPEEDIVEYTPGKIPEFKRVE FEEVNAREVFEQYDHMVGTDTVVPPGFGAAVMRIKRDGGYSLVTHSRADLALQDTYWGTL IAVLESVRKTLSVGAEPLAITNCVNYGDPDVDPVGLSAMMTALKNACEFSGVPVASGNAS LYNTYQGKPIPPTLVVGMLGKVNPQKVAKPKPSKVFAVGWNDFELEREKELWRAIRKLSE EGAFILSSSQLLTRTHVETFREYGLKIEVKLPEVRPAHQMVLVFSERTPVVDVPVKEIGT LSR*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in 283111
Recommended expression in:
Not Applicable
Vector Name:
pMH2T7
Synonyms:
pMH-2-T7
Sequencing Primer:
Forward:
pBAD forward
Reverse:
alt_bad_rev
Description:
Bacterial (E. coli) expression vector, arabinose or T7 induced expression, adds N-terminal MGSDKIHHHHHH tag; amp resistance; restriction enzyme (Pml I/Pac I) or PIPE cloning.
Comments:
None
Size (bp):
4084
Parent Vector:
None
Properties:
PIPE cloning, bacterial expression, in vitro transcription, inducible expression, with tag/fusion/marker
Author Name:
Joint Center for Structural Genomics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
ColEI origin of replication
1921
2603
promoter
T7
T7 promoter
290
310
promoter
araC
araC promoter
256
285
selectable marker
AmpR
ampicillin resistance gene
1164
1823
tag
His
N-terminal 6x His tag
367
381
trxn regulatory element
arabinose O1 operator
arabinose O1 operator
161
182
trxn regulatory element
arabinose O2 operator
arabinose O2 operator
5
23
