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Gpsm2 (Mus musculus) in pMH4 (bacterial expression vector)

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Explanation of Terms


Gene: Gene Symbol:  Gpsm2
Gene Name:  G-protein signalling modulator 2 (AGS3-like, C. elegans)
Sequence             
Original Clone ID: 11596
Keyword: full-length cds
Species: Mus musculus
Type: cDNA
Vector Name: pMH4               Format:  CLOSED
Source: Joint Center for Structural Genomics
Description: None
Comments: None
Mutation/
Discrepancy :
No / Yes
Publications: None
Authors: PSI
Joint Center for Structural Genomics
MmCD00530457

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AVAILABLE
No restriction
Special MTA: PSI

 

Insert sequence: 2019nts         Open reading frame : 51 to 2069

AGATATACATACCCATGGGATCTGATAAAATTCATCATCATCATCATCACATGAGGGAAG
ACCATTCCTTTCATGTCCGCTACAGAATGGAAGCTTCTTGCCTTGAGCTGGCCTTGGAAG
GAGAACGTCTATGTAAATCAGGGGACTGCCGCGCTGGCGTGTCATTTTTTGAAGCCGCAG
TTCAAGTTGGAACTGAAGACCTCAAAACACTCAGTGCTATTTACAGCCAACTGGGCAATG
CTTACTTCTACTTGCATGACTACGCCAAAGCGTTAGAATACCACCACCATGACTTAACTC
TTGCACGGACTATTGGAGACCAACTTGGGGAAGCTAAAGCCAGCGGCAATCTCGGAAACA
CCTTAAAGGTGCTTGGGAATTTTGATGAAGCGATAGTGTGTTGCCAGCGACACCTGGATA
TCTCCAGAGAGCTTAATGACAAGGTGGGAGAAGCAAGAGCGCTCTACAATCTTGGAAATG
TCTATCACGCCAAAGGGAAAAGCTTTGGTTGCCCGGGTCCGCAGGATACAGGAGAGTTTC
CAGAAGACGTGAGAAATGCCCTACAGGCAGCTGTGGATCTCTATGAGGAAAACCTGTCCT
TAGTAACTGCTCTAGGTGACCGAGCAGCACAGGGGCGAGCCTTTGGGAACCTTGGCAACA
CACACTACCTCCTCGGCAACTTCAGGGATGCAGTTATAGCTCACGAGCAGCGTCTCCTAA
TTGCAAAGGAGTTCGGAGATAAGGCGGCTGAGAGAAGAGCGTACAGCAACCTAGGAAATG
CATACATATTCCTGGGTGAATTCGAAACTGCTTCCGAATACTACAAGAAGACACTGCTGC
TGGCTCGGCAGCTTAAAGACAGAGCCGTGGAAGCTCAGTCTTGCTACAGTCTTGGGAATA
CATACACTTTGCTTCAGGACTACGAGAAGGCCATCGATTACCACCTAAAGCACTTAGCAA
TCGCCCAAGAGCTCAAGGATCGGATTGGTGAAGGAAGAGCGTGTTGGAGCTTAGGAAACG
CATACACAGCTCTGGGAAATCATGACCAAGCAATGCATTTTGCTGAAAAGCACTTGGAAA
TTTCAAGAGAGGTTGGGGACAAAAGTGGTGAACTGACTGCGCGGCTGAACCTCTCAGACC
TGCAGATGGTTCTTGGTCTGAGCTACAGCACAAATAATTCCATGATGTCTGAGAACATTG
AAATTGATGGCAGCTTACATGGTGCAGGCGCCAAGCTGGGACGGAGACACAGTATGGAGA
ACTTGGAACTTATGAAGTTAACACCAGAAAAGGTGCCGAATTGGAACAGTGAAATTCTCG
CTAAACAGAAACCTCTCATTGCCAAACCATCTGCAAAGCTCCTGTTTGTCAACAGACTGA
AGGGCAAAAAGTACAAAAGCGGCAGCGCCTGCACTAAGGTTCTCCAAGACGCCAGCAACT
CCGTGGACCACCGCGCGCCACGCTCCCAGAAGAAAATCAGTTCAGACACGATTGGAGATG
AAGGATTCTTTGACTTGCTAAGGCGATTTCAGAGCAACAGGATGGATGACCAGAGGTGCC
ACTTACAAGGAAACTGTCGCACAACATCCACAGCAGCTGCTTCTGCTACCCCCAAGTTGA
TGAAAGCACCGTCTGTCTCTGTGGTGTCCCCCAACACAGATGAGTTCTTAGATCTTCTTG
CTAGCTCACAGAGCCGCCGTCTGGATGACCAGAGGGCCAGTTTCAGTAATTTGCCAGGGC
TCCGCCTGACAAAAGGCAACAGTCCATCTGTACTTGAGCGCCTGATGACAAATGACAAGA
AAGAGCCTGATGAAGACTTCTTCGACATCCTTGTAAAGTGCCAGGGGTCAAGATTAGATG
ATCAAAGATGTGCTCCTCCATCTGCTGCCACTAAGGGGCCGACTGTCCCGGATGAGGACT
TCTTTAGCCTCATCTTACGCTCTCAAGCTAAAAGAATGGATGAGCAGAGAGTTCTGCTGC
AAAGAGATCCAAACAGAGACAGTGAGTTTGGACTAAAGGAACTTTTGCAAAATAACGCTT
TGTTGGAATTTAAGCATTCGGGAAAATAACGGGACTTAATTAACTCTAGATAACTGAGAA
GGAGATATACATACCCGGGT


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
ATGGGATCTGATAAAATTCATCATCATCATCATCAC

3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: ProteinExpressed: Not_Applicable
ProteinPurified: Protein_Purified
SolubleProtein: Tested_Not_Soluble
Find experimental protocols in 354767
Recommended expression in: HK100
       DyNA Vector Map

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Vector Name: pMH4

pMH4 in advanced viewed

Synonyms: pMH-4
Sequencing Primer: Forward:  pBAD forward
Reverse:  alt_bad_rev
Description: Bacterial expression vector, arabinose or T7 induced expression, adds N-terminal MGSDKIHHHHHH tag; amp resistance; restriction enyzme (Pml I/Pac I) or PIPE cloning.
Comments: None
Size (bp): 5489
Parent Vector: None
Empty Vector: None
Properties: PIPE cloning, bacterial expression, in vitro transcription, inducible expression, with tag/fusion/marker
Author Name: Joint Center for Structural Genomics
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori ColE1 bacterial origin of replication 3331 4013
inducible trxn activator protein gene araC araC cds 5463 4585
negative selection marker ccdB ccdB negative selection gene (may be gone in with-insert form) 1576 1785
promoter T7 T7 promoter 290 310
promoter araC araC promoter 153 124
selectable marker CmR chloramphenicol resistance gene (CmR) 484 1140
selectable marker AmpR ampicillin resistance gene 2574 3233
tag His N-terminal 6xHis tag 364 381
trxn regulatory element arabinose O1 operator arabinose O1 operator 161 182
trxn regulatory element arabinose O2 operator arabinose O2 operator 5 23
trxn termination sequence rrnB rrnB T2 transcription terminator 2238 2264

 

  • Gene
  • Protein
  • Clone
  • Experimental
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : Gpsm2
Symbol Nomenclature : Gpsm2
SYNONYM : 6230410J09Rik; LGN; Pins
Designation : G-protein-signaling modulator 2|pins homolog (Drosophia)
Full Nomenclature : G-protein signalling modulator 2 (AGS3-like, C. elegans)
GENEID : 76123
Locus Tag : RP23-217I18.2
GI : 164519124
GenBank Accession : NM_029522
Mouse Genome Informatics : 1923373
Vega : OTTMUSG00000007258
Target GenBank: None

 

Clone

Cloning Information : 277797
HIP Master Clone ID : 207283
Original Clone ID : 11596

Experimental

TargetTrack - Experimental data : 354767

Organism


TAX_ID : 10090
Species Specific ID: 76123

Genome

Chromosome : 3
Map Location : 3 F3|3
Ensembl : ENSMUSG00000027883

Reagents

Labome : Gpsm2-antibody