DR_2108 (D. radiodurans )
In pET21_NESG (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  DR_2108
Gene Name:  molybdate metabolism regulator-like protein
Original Clone ID:
DrR64-21.2
Keyword:
None
Species:
Deinococcus radiodurans
Type:
cDNA
Vector Name:
pET21_NESG
Format: 
CLOSED
Source:
Northeast Structural Genomics Consortium
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
PSI
Northeast Structural Genomics Consortium
DrCD00530719
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AVAILABLE
No restriction
Special MTA: PSI/NESG
Insert sequence: 1440nts
Open reading frame: 1 to 1440

ATGACCGGTGTATTGTCGGGTATGGCGAAAACTTATCTGGAGTTCTCCGACCCCAACGGC
GCGGAGCATAAGTTTTACGAGGTGACGGTGGACGGTCCCGAGCTGACCATCCGTTACGGG
CGCATCGGCACGGACGGTCAGCGCAGCACCAAGACCTTCCCCGACGCGGCGGTCGCACAG
GCCGAAGCCGACAAGAAAATCAGAGAGAAGCGCAAAAAAGGCTACGAGGACGCCGTGATG
GGCGCGCGTCAAAAGCGGACCGTCGTGCGCCGCGAGGTCACCGAGACGCGCAGCACCACC
AGGCAGATTGCGCCGGTGTTGTGGCGCTTCAAGACCCGCAGCGCCGCCTACGGCATCTTC
GTGGATCAGAACACGGCCTGGGTGGGGAACGACTCCGGCGAGGTCTTCGCACTGACGCTC
TCGGGCGAGGTCGACCGCTCGTTCCGTTTGCCGGACGGCGTCAAATGTCTCGTGAGCGAC
CAGTTCCGCACGTTCGCAGGTTGCGACGACGGCAACGTGTACGACCTGAGCGGCAAGCTC
CCCTTCATGGCCTATGAAGTCGAGAGCAAGGCAGCCCTGCTGTGGCTCGACCTGCACGGC
GGCACCCTGGGCGTGTCCGACAGCGGGGGCGGGGTGTACGCTTTCGATGCCGAGAGCGAC
CAGCAGTGGGGCCACACGGACGACCGGGCCAGCATGGGCTGGATGGTGCGCGTGGACGCT
CGGGGCGTGTACTACGGTCACTCGCGCGGCGTGGGCATGTTCGACCGGGAAAGCGGTTTC
CCGAACTGGCACCGGCCGACGCGGGGCAGCGTGCTGTTCGGCTGGCAGGAGGGCAACGAC
CTGTACGCCGGAACCAGCGCGAACCTCATTCAGCGCTTCACGAAGGCCGGGGAGCACGTG
CAGGACTATCAATGCGACGGCAGCGTGCTCTCGTGCGCCGCCAGTCCCGGCGGGGAGTTC
GTGTTTGCAGGCGACAGCGGCAGCGCCGTGTACTGTTTCAACCGCGCCGGCGAGCGTCTG
TGGAAACTGGGAACCGGCTGCGCCTCGGCCCTGAGCATGCAATTCGCCGCCGACAAGCTC
TACATCGTGACCACGAACGGCACGCTCGCCGCCATCGATGTGAGCCCGGCAGCCATCGAA
GCCGCCAGGCAGGGCACGACGCCAGAGGTCCGTGACGTGAAGCTGGCCGCAGCCATGCCG
GCCCAAGCACCCGCCACCGAACTGACCGTCACCCGGAACGCCAGCGGCGGCGTGCTGCTG
CGCGGCGTGCAGGAAGGCAGCAAGGTCCGTATTCGCCCCGACGACTCACGCTTTCATAAA
GACTGGAACGTGCAGTTCCCCCGCAATCTGCGCGCAGCAGACGGACTCTACGTGGTGGAC
GACCTGGAGGACGCGGGCGGCTTCTACCGTGTGGTGGGCGATCTCCGACGCCTGGAAAAC

Protein sequence predicted based on nucleotide sequence

MTGVLSGMAKTYLEFSDPNGAEHKFYEVTVDGPELTIRYGRIGTDGQRSTKTFPDAAVAQ
AEADKKIREKRKKGYEDAVMGARQKRTVVRREVTETRSTTRQIAPVLWRFKTRSAAYGIF
VDQNTAWVGNDSGEVFALTLSGEVDRSFRLPDGVKCLVSDQFRTFAGCDDGNVYDLSGKL
PFMAYEVESKAALLWLDLHGGTLGVSDSGGGVYAFDAESDQQWGHTDDRASMGWMVRVDA
RGVYYGHSRGVGMFDRESGFPNWHRPTRGSVLFGWQEGNDLYAGTSANLIQRFTKAGEHV
QDYQCDGSVLSCAASPGGEFVFAGDSGSAVYCFNRAGERLWKLGTGCASALSMQFAADKL
YIVTTNGTLAAIDVSPAAIEAARQGTTPEVRDVKLAAAMPAQAPATELTVTRNASGGVLL
RGVQEGSKVRIRPDDSRFHKDWNVQFPRNLRAADGLYVVDDLEDAGGFYRVVGDLRRLEN

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
AAGGAGATATACAT
3' Linker Sequence:
CTCGAGCACCACCACCACCACCACTGA

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinPurified: Not_Tested
ProteinExpressed: Protein_Confirmed
SolubleProtein: Protein_Soluble
Find experimental protocols in DrR64
Recommended expression in:
Not Applicable