DNASU Plasmid | Wdr37 (M. musculus )<br>In pSGX3 (His-tagged bacterial expression vector)

Explanation of Terms

Gene:

Gene Symbol: Wdr37

Gene Name: WD repeat domain 37

Original Clone ID:

13121a1BCt7p1

Keyword:

full-length cds

Species:

Mus musculus

Type:

cDNA

Vector Name:

pSGX3

Format:

CLOSED

Source:

New York SGX Research Center for Structural Genomics

Description:

None

Comments:

None

Mutation / Discrepancy:

Yes / No

Publications:

None

Authors:

PSI
New York SGX Research Center for Structural Genomics

MmCD00531589

Price:

AVAILABLE

No restriction
Special MTA: PSI

Insert Sequence
Protein Sequence

Insert sequence: 1509nts

Open reading frame: 1 to 1509

ACGGAAAGCGGTTCGTGTAGCACGGCTCGTCAGGCAAAACAAAAGCGTAAGAGCCACAGC
CTGAGCATTCGTCGTACCAATAGCTCCGAGCAGGAACGTACTGGTTTGCCACGTGAAATG
CTGGAGGGCCAGGACAGCAAGTTGCCGTCCTCTGTTCGCAGCACCTTGCTGGAACTGTTC
GGTCAGATCGAACGCGAATTCGAGAATCTGTATATCGAGAATCTGGAGCTGCGTCGCGAA
ATCGATACTCTGAACGAGCGTCTGGCCGGTGAAGGTCAGGCGATTGACGGCGCAGAGCTG
TCCAAAGGCCAGTTGAAAACGAAAGCAAGCCACAGCACCTCCCAGCTGAGCCAGAAGCTG
AAAACGACTTACAAAGCGTCCACGTCTAAGATCGTCAGCAGCTTTAAGACGACCACCAGC
CGCGCAATCTGTCAACTGGTCAAAGAGTACATCGGTCACCGCGATGGTATCTGGGATGTG
AGCGTCACCCGCACCCAGCCGATCGTGCTGGGTACCGCGTCTGCCGATCATACCGCAATG
CTGTGGAGCATCGAGACGGGTAAATGCCTGGTGAAGTATGCGGGTCACGTGGGTAGCGTG
AACAGCATTAAGTTCCACCCTAGCGAGCAGCTGGCACTGACCGCGAGCGGTGACCAAACC
GCGCATATCTGGCGCTACGTGGTTCAGCTGCCGACGCCGCAACCGGTGGCGGACACGAGC
CAACAAATCAGCGGCGAAGACGAGATTGAGTGCAGCGACAAGGATGAGCCAGATATCGAT
GGCGACGTGAGCAGCGACTGTCCAACGGTCCGCGTGCCTCTGACGTCGCTGAAAAGCCAC
CAAGGTGTTGTGATTGCAGCGGACTGGCTGGTGGGTGGCAAACAGGTCGTTACTGCGTCC
TGGGATCGCACTGCTAACATGTATGACGTGGAAACCAGCGAGTTGGTTCATAGCCTGACC
GGCCATGATCAGGAACTGACCCATTGCTGCACCCACCCGACGCAACGTCTGGTGGTGACC
AGCTCCCGCGACACGACCTTTCGTCTGTGGGACTTCCGCGATCCGAGCATTCATTCCGTT
AACGTTTTCCAGGGCCATACGGACACTGTCACCAGCGCTGTCTTTACCGTGGGCGACAAT
GTGGTGTCCGGTAGCGACGATCGCACGGTTAAAGTCTGGGATCTGAAGAATATGCGTAGC
CCGATCGCTACCATCCGTACCGATAGCGCAATCAACCGTATCAACGTCTGCGTTGGTCAG
AAAATCATTGCGTTGCCGCACGATAATCGTCAAGTGCGTCTGTTTGACATGAGCGGTGTT
CGTCTGGCGCGTTTGCCGCGTAGCAGCCGTCAGGGTCATCGTCGTATGGTCTGCTGTAGC
GCGTGGAGCGAGGATCATCCGATTTGCAACCTGTTCACCTGTGGCTTTGATCGTCAAGCC
ATTGGTTGGAACATTAACATTCCGGCGCTGCTGCAAGAGAAAGAAGGGCATCATCACCAT
CACCACTGA

Protein sequence predicted based on nucleotide sequence

TESGSCSTARQAKQKRKSHSLSIRRTNSSEQERTGLPREMLEGQDSKLPSSVRSTLLELF
GQIEREFENLYIENLELRREIDTLNERLAGEGQAIDGAELSKGQLKTKASHSTSQLSQKL
KTTYKASTSKIVSSFKTTTSRAICQLVKEYIGHRDGIWDVSVTRTQPIVLGTASADHTAM
LWSIETGKCLVKYAGHVGSVNSIKFHPSEQLALTASGDQTAHIWRYVVQLPTPQPVADTS
QQISGEDEIECSDKDEPDIDGDVSSDCPTVRVPLTSLKSHQGVVIAADWLVGGKQVVTAS
WDRTANMYDVETSELVHSLTGHDQELTHCCTHPTQRLVVTSSRDTTFRLWDFRDPSIHSV
NVFQGHTDTVTSAVFTVGDNVVSGSDDRTVKVWDLKNMRSPIATIRTDSAINRINVCVGQ
KIIALPHDNRQVRLFDMSGVRLARLPRSSRQGHRRMVCCSAWSEDHPICNLFTCGFDRQA
IGWNINIPALLQEKEGHHHHHH*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

L182M; L309M

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: kanamycin

Bacterial Selection Condition: 50ug/mL kanamycin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.

Protein Expression Results:

ProteinPurified: Not_Tested

ProteinExpressed: Protein_Confirmed

SolubleProtein: Tested_Not_Soluble

Find experimental protocols in NYSGXRC-13121a

Recommended expression in:

Not Applicable

Vector Name:

pSGX3

Synonyms:

pSGX-3, pSGX3_BC

Sequencing Primer:

Forward: T7

Reverse: T7 terminator

Description:

Bacterial (E. coli) expression vector with T7 promoter, adds C-terminal 6xHis tag; kanamycin resistance; TOPO cloning.

Comments:

Modified from pET26b from Novagen

Size (bp):

5339

Parent Vector:

None

Properties:

TOPO Cloning, bacterial expression, in vitro transcription, with tag/fusion/marker

Author Name:

New York SGX Research Center for Structural Genomics

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

TGGCGAATGGGACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGTTTACAATTTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAATTAATTCTTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGGATTATCAATACCATATTTTTGAAAAAGCCGTTTCTGTAATGAAGGAGAAAACTCACCGAGGCAGTTCCATAGGATGGCAAGATCCTGGTATCGGTCTGCGATTCCGACTCGTCCAACATCAATACAACCTATTAATTTCCCCTCGTCAAAAATAAGGTTATCAAGTGAGAAATCACCATGAGTGACGACTGAATCCGGTGAGAATGGCAAAAGTTTATGCATTTCTTTCCAGACTTGTTCAACAGGCCAGCCATTACGCTCGTCATCAAAATCACTCGCATCAACCAAACCGTTATTCATTCGTGATTGCGCCTGAGCGAGACGAAATACGCGATCGCTGTTAAAAGGACAATTACAAACAGGAATCGAATGCAACCGGCGCAGGAACACTGCCAGCGCATCAACAATATTTTCACCTGAATCAGGATATTCTTCTAATACCTGGAATGCTGTTTTCCCGGGGATCGCAGTGGTGAGTAACCATGCATCATCAGGAGTACGGATAAAATGCTTGATGGTCGGAAGAGGCATAAATTCCGTCAGCCAGTTTAGTCTGACCATCTCATCTGTAACATCATTGGCAACGCTACCTTTGCCATGTTTCAGAAACAACTCTGGCGCATCGGGCTTCCCATACAATCGATAGATTGTCGCACCTGATTGCCCGACATTATCGCGAGCCCATTTATACCCATATAAATCAGCATCCATGTTGGAATTTAATCGCGGCCTAGAGCAAGACGTTTCCCGTTGAATATGGCTCATAACACCCCTTGTATTACTGTTTATGTAAGCAGACAGTTTTATTGTTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATACACTCCGCTATCGCTACGTGACTGGGTCATGGCTGCGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGGCAGCTGCGGTAAAGCTCATCAGCGTGGTCGTGAAGCGATTCACAGATGTCTGCCTGTTCATCCGCGTCCAGCTCGTTGAGTTTCTCCAGAAGCGTTAATGTCTGGCTTCTGATAAAGCGGGCCATGTTAAGGGCGGTTTTTTCCTGTTTGGTCACTGATGCCTCCGTGTAAGGGGGATTTCTGTTCATGGGGGTAATGATACCGATGAAACGAGAGAGGATGCTCACGATACGGGTTACTGATGATGAACATGCCCGGTTACTGGAACGTTGTGAGGGTAAACAACTGGCGGTATGGATGCGGCGGGACCAGAGAAAAATCACTCAGGGTCAATGCCAGCGCTTCGTTAATACAGATGTAGGTGTTCCACAGGGTAGCCAGCAGCATCCTGCGATGCAGATCCGGAACATAATGGTGCAGGGCGCTGACTTCCGCGTTTCCAGACTTTACGAAACACGGAAACCGAAGACCATTCATGTTGTTGCTCAGGTCGCAGACGTTTTGCAGCAGCAGTCGCTTCACGTTCGCTCGCGTATCGGTGATTCATTCTGCTAACCAGTAAGGCAACCCCGCCAGCCTAGCCGGGTCCTCAACGACAGGAGCACGATCATGCGCACCCGTGGGGCCGCCATGCCGGCGATAATGGCCTGCTTCTCGCCGAAACGTTTGGTGGCGGGACCAGTGACGAAGGCTTGAGCGAGGGCGTGCAAGATTCCGAATACCGCAAGCGACAGGCCGATCATCGTCGCGCTCCAGCGAAAGCGGTCCTCGCCGAAAATGACCCAGAGCGCTGCCGGCACCTGTCCTACGAGTTGCATGATAAAGAAGACAGTCATAAGTGCGGCGACGATAGTCATGCCCCGCGCCCACCGGAAGGAGCTGACTGGGTTGAAGGCTCTCAAGGGCATCGGTCGAGATCCCGGTGCCTAATGAGTGAGCTAACTTACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAGCTGTCTTCGGTATCGTCGTATCCCACTACCGAGATATCCGCACCAACGCGCAGCCCGGACTCGGTAATGGCGCGCATTGCGCCCAGCGCCATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCCCTCATTCAGCATTTGCATGGTTTGTTGAAAACCGGACATGGCACTCCAGTCGCCTTCCCGTTCCGCTATCGGCTGAATTTGATTGCGAGTGAGATATTTATGCCAGCCAGCCAGACGCAGACGCGCCGAGACAGAACTTAATGGGCCCGCTAACAGCGCGATTTGCTGGTGACCCAATGCGACCAGATGCTCCACGCCCAGTCGCGTACCGTCTTCATGGGAGAAAATAATACTGTTGATGGGTGTCTGGTCAGAGACATCAAGAAATAACGCCGGAACATTAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGATCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCACCGCCGCTTTACAGGCTTCGACGCCGCTTCGTTCTACCATCGACACCACCACGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCCGGGATCTCGACGCTCTCCCTTATGCGACTCCTGCATTAGGAAGCAGCCCAGTAGTAGGTTGAGGCCGTTGAGCACCGCCGCCGCAAGGAATGGTGCATGCAAGGAGATGGCGCCCAACAGTCCCCCGGCCACGGGGCCTGCCACCATACCCACGCCGAAACAAGCGCTCATGAGCCCGAAGTGGCGAGCCCGATCTTCCCCATCGGTGATGTCGGCGATATAGGCGCCAGCAACCGCACCTGTGGCGCCGGTGATGCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCTCGATCCCGCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGTCCCTTAAGGGCATCATCACCATCACCACTGATCCCACCACCACCACCACCACTGAGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGCACCACCACCACCACCACTGAGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAAGGAGGAACTATATCCGGAT

Vector Features:

Type

Name

Description

Start

End

ATG start

start

initiation start (Met, Ser, Leu)

5072

5079

TOPO recognition site

TOPO

TOPO cloning site

5076

5085

bacterial origin

ori

pBR322-type bacterial origin of replication

1421

2103

gene fragment

ROP

2527

2703

promoter

T7 promoter

4983

5003

repressor protein gene

LacI

LacI CDS

3515

4531

selectable marker

KanR

kanamycin resistance gene

563

1375

tag

His

6xHis tag

5086

5103

trxn regulatory element

lac operator

5003

5025

trxn termination sequence

T7 term

T7 terminator

5231

5307

Gene

Gene Symbol :

Wdr37

Symbol Nomenclature :

Wdr37

Designation :

WD repeat-containing protein 37

Full Nomenclature :

WD repeat domain 37

GENEID :

207615

GI :

86476059

GenBank Accession :

NM_001039388

Mouse Genome Informatics :

1920393

Vega :

OTTMUSG00000042174

Target GenBank:

None

Protein

SMART domain :

WD40 : WD40 repeats

Structural Annotation Wiki :

NYSGXRC-13121a

Clone

Cloning Information :

483761

HIP Master Clone ID :

401489

Original Clone ID :

13121a1BCt7p1

Experimental

TargetTrack - Experimental data :

NYSGXRC-13121a

Organism

Species Specific ID:

207615

Genome

Chromosome :

Map Location :

13 A1|13

Ensembl :

ENSMUSG00000021147

Reagents

Labome :

Wdr37-antibody

Wdr37 (M. musculus )In pSGX3 (His-tagged bacterial expression vector)

Coding Sequence Details

Recommended Growth Condition:

Vector Features:

Gene

Protein

Clone

Experimental

Organism

Genome

Reagents

Wdr37 (M. musculus )
In pSGX3 (His-tagged bacterial expression vector)