Gene:
Gene Symbol:
NMB0075
Gene Name:
transcriptional accessory protein Tex
Original Clone ID:
6019o1NYt2b2
Keyword:
None
Species:
Neisseria meningitidis
Type:
cDNA
Vector Name:
pNYSGXRC
Format:
CLOSED
Source:
New York SGX Research Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
New York SGX Research Center for Structural Genomics
New York SGX Research Center for Structural Genomics
NpCD00534580
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AVAILABLE
No restriction
Special MTA: PSI
Special MTA: PSI
Insert sequence: 2271nts
Open reading frame: 51 to 2321
AGGATAACGATATTATTGAGGCTCACAGAGAACAGATTGGTGGATCCCTTAACATTACTC
AAATTCTTTCCCAAGAACTCTCCGCGACTGCCGCGCAAATCACCGCCGCCGTCGAGCTTT
TGGACGACGGCGCGACCGTGCCGTTTATCGCCCGCTACCGCAAGGAAGCGACGGGCGGGT
TGGACGATACGCAGTTGCGCCGGCTTGCCGAGCGGCTGCAATATCTGCGCGAGTTGGAAG
AGCGCAAAGCCGTTGTTTTAAAAAGCATTGAAGAGCAAGGCAAGCTTTCAGACGACCTCA
GGGCGCAAATCGAAGCCGCCGATAACAAAACCGCGCTGGAAGACCTGTATCTGCCCTACA
AACCCAAACGCCGCACCAAAGCGCAAATCGCGCGCGAACACGGTTTGCAGCCGCTGGCGG
ACGTGTTGCTTGCCGAGCAGTCGCAGGACGTGGAAGCCGCCGCACAAGGCTACCTGAACG
AAAACGTCCCCGATGCCAAAGCCGCGTTGGACGGCGCGCGTGCGATTCTGATGGAGCAGT
TTGCCGAAGACGCGGAACTTATCGGCACGCTGCGCGACAAGCTGTGGAACGAAGCCGAAA
TCCACGCGCAAGTCGTTGAAGGCAAAGAAACCGAAGGCGAAAAATTCAGCGATTATTTCG
ACCACCGCGAACCCGTCCGCACTATGCCCAGCCACCGCGCGCTGGCGGTTTTGCGCGGCC
GCAACGAAGGCGTGTTGAACATCGCGCTCAAATACCAGCCCGACGACACGCCGATTACCC
GGCAAAGCGAATACGAGCAAATCATCGCCTGCCGCTTCAAGGTTTCAGACGGCCACAAAT
GGCTGCGCGATACCGTGCGTCTGACTTGGCGCGCGAAAATCTTTTTGTCGTTGGAACTTG
AAGCCCTAGGCCGTCTGAAAGAAGCCGCCGACACCGACGCGATTACCGTGTTCGCCCGCA
ATCTCAAAGACTTGCTGCTCGTCGCGCCCGCCGGACGGCTGACCACGCTGGGTCTCGACC
CCGGCTACCGCAACGGCGTGAAATGCGCCGTGGTGGACGACACCGGCAAGCTGCTGGATA
CCGTCATCGTCTATTTGCATCAAGAAAACAATATGTTGGCAACGCTGTCGCGCCTGATTA
AGCAACACGGCGTGAAGCTCATCGCCATCGGCAACGGCACCGCCAGCCGCGAAACCGACA
AAATCGCGGGCGAACTGGTGCGCGGAATGCCGGAAATGGGGCTGCACAAAATCGTCGTGT
CCGAAGCCGGCGCGTCGATTTATTCCGCGTCCGAACTGGCGGCGCGCGAGTTCCCCGACT
TGGACGTTTCCCTGCGCGGCGCGGTGTCCATCGCCCGCAGGCTGCAAGACCCGCTTGCCG
AGTTGGTCAAAATCGACCCTAAATCCATCGGCGTGGGCCAGTATCAGCACGATGTGAACC
AAAACCAGCTCGCCAAATCGCTGGACGCAGTGGTCGAAGACTGCGTGAACGCCGTCGGCG
TGGACGTGAATACCGCCTCCGCCCCGCTCTTGGCGCGGATTTCCGGCTTGAATCAAACCC
TTGCCCAAAACATCGTTGCCTACCGCGATGAAAACGGCGCGTTCGACAGCCGCAAAAAAT
TGCTGAAAGTACCGCGTTTGGGCGAAAAAACCTTCGAGCAGGCGGCAGGCTTTTTGCGGA
TTAACGGCGGTAAAGAGCCGTTGGACGCGAGCGCCGTCCACCCCGAAGCCTATCCCGTCG
TCGCCAAAATGCTGGCGCAACAAGGCATTAGCGCCGCCGAACTCATCGGCAACCGCGAGC
GCGTGAAGCAAATCAAAGCGTCCGACTTCACCGACGAACGCTTCGGCCTGCCGACCATTT
TGGACATCCTGTCCGAACTGGAAAAACCCGGCCGTGATCCGCGCGGCGAGTTTCAGACGG
CATCGTTTGCCGAAGGTATCCACGAAATCAGCGACTTGCAAGTCGGTATGATACTCGAAG
GCGTGGTTTCCAACGTCGCCAACTTCGGCGCGTTCGTGGACATCGGCGTCCATCAGGACG
GCTTGGTGCACATCTCCGCCCTGTCCAACAAGTTCGTCCAAGACCCGCGCGAAGTGGTGA
AAGCTGGCGACGTGGTGAAAGTGAAAGTGCTGGAAGTCGATGCTGCACGCAAACGCATCG
CGCTGACCATGCGCTTGGATGACGAACCGGGCGGCGCAAAACATAAAATGCCGTCTGAAA
ACCGCAGCCGCGAACGGACAGCCGGCCGCAAACCCCAACGCAACGACCGCGCCCCAGCCA
ATTCGGCGATGGCGGATGCGTTTGCGAAGCTGAAGCGGTAAGGTGAAGGGGCGGCCGCAC
TCGAGCACCACCACCACCACCACTGAGATCC
Protein sequence predicted based on nucleotide sequence
NITQILSQELSATAAQITAAVELLDDGATVPFIARYRKEATGGLDDTQLRRLAERLQYLR ELEERKAVVLKSIEEQGKLSDDLRAQIEAADNKTALEDLYLPYKPKRRTKAQIAREHGLQ PLADVLLAEQSQDVEAAAQGYLNENVPDAKAALDGARAILMEQFAEDAELIGTLRDKLWN EAEIHAQVVEGKETEGEKFSDYFDHREPVRTMPSHRALAVLRGRNEGVLNIALKYQPDDT PITRQSEYEQIIACRFKVSDGHKWLRDTVRLTWRAKIFLSLELEALGRLKEAADTDAITV FARNLKDLLLVAPAGRLTTLGLDPGYRNGVKCAVVDDTGKLLDTVIVYLHQENNMLATLS RLIKQHGVKLIAIGNGTASRETDKIAGELVRGMPEMGLHKIVVSEAGASIYSASELAARE FPDLDVSLRGAVSIARRLQDPLAELVKIDPKSIGVGQYQHDVNQNQLAKSLDAVVEDCVN AVGVDVNTASAPLLARISGLNQTLAQNIVAYRDENGAFDSRKKLLKVPRLGEKTFEQAAG FLRINGGKEPLDASAVHPEAYPVVAKMLAQQGISAAELIGNRERVKQIKASDFTDERFGL PTILDILSELEKPGRDPRGEFQTASFAEGIHEISDLQVGMILEGVVSNVANFGAFVDIGV HQDGLVHISALSNKFVQDPREVVKAGDVVKVKVLEVDAARKRIALTMRLDDEPGGAKHKM PSENRSRERTAGRKPQRNDRAPANSAMADAFAKLKR*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Tested_Not_Purified
SolubleProtein: Tested_Not_Soluble
Find experimental protocols in NYSGXRC-6019o
Recommended expression in:
Not Applicable
Vector Name:
pNYSGXRC
Synonyms:
pNYSGXRC_NY
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Bacterial (E. coli) expression vector for directionaly cloning, similar to BS vector; kanamycin resistance; directional cloning (restriction enzyme cloning).
Comments:
Supplied by Chris Lima for the NYSGXRC.
Size (bp):
5759
Parent Vector:
None
Properties:
bacterial expression, multiple cloning site
Author Name:
New York SGX Research Center for Structural Genomics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
ColE1-type origin of replication
1554
2236
promoter
T7
T7 promoter (for Smt2p region)
5117
5136
repressor protein gene
LacI
LacI coding region
3651
4730
selectable marker
AmpR
ampicillin resistance gene
797
1456
ssDNA origin
F1 ori
F1 origin for ssDNA production
457
17
tag
His
6xHis tag
5216
5233
tag
Smt3
S. cerevisiae Smt3p cds
5204
5584
trxn regulatory element
lac operator
lac operator
5136
5158
trxn termination sequence
T7 term
T7 terminator sequence (after Smt3p region)
5688
5734
