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Detailed Vector Information: pGEc2-DEST

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Explanation of Terms


Gene: Gene Symbol:  No insert
Gene Name:  No insert
Original Clone ID: None
Type: No insert
Vector Name: pGEc2-DEST              
Source: Kelley Moremen
Description: Mammalian expression vector with a CMV promoter and C-terminal Superfolder GFP, AviTag and 10xHis tag; ampicillin resistance in bacteria; Gateway recombinational cloning
Comments: Empty vector contains ccdB death cassette, which is removed in constructs containing a gene insert
Publications: None
Authors: University of Georgia
Kelley Moremen
EvNO00534627

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AVAILABLE
No restriction
Special MTA: SPTA

 

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
       DyNA Vector Map

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Vector Name: pGEc2-DEST

pGEc2-DEST in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  pGEc1-DEST F
Reverse:  pGEn1-DEST R
Description: Mammalian expression vector with a CMV promoter and C-terminal Superfolder GFP, AviTag and 10xHis tag; ampicillin resistance in bacteria; Gateway recombinational cloning
Comments: None
Size (bp): 6852
Parent Vector: Name: pXKM
Description:
Properties: Gateway, acceptor (destination), fluorescent marker, mammalian transduction, recombinational cloning, with tag/fusion/marker
Author Name: University of Georgia
Kelley Moremen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 origin 1137 1804
death cassette ccdB ccdB death cassette (removed in plasmids with insert) 4211 4516
intron artificial intron artificial intron 2828 2960
poly-A signal BGH pA bovine growth hormone (BGH) polyadenylation signal 6028 6242
promoter CMV CMV promoter for expression in mammalian cells 2208 2790
recombination site attR2 attR2 recombinational cloning site 4557 4681
recombination site attR1 attR1 recombinational cloning site 2974 3101
recombination site 3'-ITR inverted terminal repeat sequence 6330 6459
selectable marker AmpR ampicillin resistance 129 989
selectable marker CmR Chloramphenicol resistance (CmR) gene (removed in plasmids with insert) 3210 3859
tag AviTag C-terminal AviTag 5378 5428
tag GFP C-terminal super GFP 4682 5377
tag His C-terminal 10xHis tag 5429 5452
trxn regulatory element WPRE woodchuck hepatitis post-transcriptional regulatory element 5480 6021

 

Species Specific ID: None
Target GenBank: None