DNASU Plasmid | HIRA (Homo sapiens) in pSpeedET (bacterial expression vector)

Explanation of Terms

Gene:

Gene Symbol: HIRA

Gene Name: histone cell cycle regulator

Original Clone ID:

85551

Keyword:

partial cds

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pSpeedET

Format:

CLOSED

Source:

Joint Center for Structural Genomics

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

PSI:Biology
Joint Center for Structural Genomics

HsCD00545876

Price:

AVAILABLE

No restriction
Special MTA: PSI

Insert Sequence
Protein Sequence

Insert sequence: 1218nts

Open reading frame: 51 to 1268

Reference Sequence Alignment

CTGATAAAATTCATCATCATCATCATCACGAAAACCTGTACTTCCAGGGCATGAAGCTCC
TGAAGCCGACCTGGGTCAACCACAATGGCAAGCCGATTTTTTCAGTTGATATTCACCCTG
ACGGGACCAAGTTCGCAACTGGAGGACAAGGGCAGGATTCTGGGAAGGTTGTGATCTGGA
ATATGTCTCCAGTCCTCCAGGAGGATGACGAGAAGGATGAAAATATTCCCAAGATGCTTT
GCCAGATGGACAATCACTTAGCATGTGTGAACTGTGTGCGGTGGTCAAACAGTGGGATGT
ATTTAGCTTCTGGGGGAGATGACAAACTGATTATGGTGTGGAAGCGGGCTACGTACATCG
GCCCCAGCACCGTGTTCGGCTCCAGTGGTAAGCTTGCCAATGTGGAGCAGTGGCGGTGTG
TCTCTATCCTCCGGAATCATTCAGGCGATGTGATGGATGTAGCATGGTCTCCCCACGATG
CCTGGCTAGCCTCATGCAGCGTGGATAACACTGTCGTCATCTGGAATGCTGTAAAGTTCC
CAGAAATTCTAGCTACTCTGAGAGGTCATTCTGGCTTGGTCAAAGGGTTGACATGGGACC
CTGTTGGTAAATACATAGCTTCTCAAGCTGATGACCGCAGCCTAAAGGTGTGGAGGACGC
TGGACTGGCAGTTGGAGACCAGCATCACCAAGCCTTTTGATGAGTGTGGAGGAACGACCC
ATGTGTTGCGGCTCAGCTGGTCACCTGATGGGCATTACCTGGTGTCTGCCCATGCCATGA
ACAACTCAGGCCCCACTGCCCAGATCATCGAACGGGAGGGATGGAAGACCAACATGGACT
TTGTTGGGCACCGGAAAGCTGTGACTGTCGTGAAATTCAACCCAAAAATCTTCAAAAAGA
AGCAGAAGAATGGGAGTTCTGCGAAGCCTAGCTGCCCGTACTGCTGCTGTGCTGTTGGCA
GCAAGGACCGCTCGCTTTCTGTCTGGCTCACATGTCTGAAACGGCCGCTGGTGGTCATCC
ATGAACTGTTTGACAAATCCATCATGGATATTTCCTGGACTCTGAATGGGCTGGGCATCT
TGGTATGCTCTATGGACGGCTCTGTGGCATTCCTCGACTTCTCCCAGGATGAGCTTGGCG
ATCCCCTGAGCGAGGAGGAGAAGAGCCGCATTCACCAGTCCACCTATGGCAAGAGCCTAG
CCATCATGACCGAGGCCCAGCTCTCCACAGCCGTCATTGAGAACCCTGAGATGCTCAAGT
ACCAGTAACGCGACTTAATTAACTCGTTTAAACGGTCTCCAGCTTGGCTGTTTTGGCGG

Protein sequence predicted based on nucleotide sequence

MKLLKPTWVNHNGKPIFSVDIHPDGTKFATGGQGQDSGKVVIWNMSPVLQEDDEKDENIP
KMLCQMDNHLACVNCVRWSNSGMYLASGGDDKLIMVWKRATYIGPSTVFGSSGKLANVEQ
WRCVSILRNHSGDVMDVAWSPHDAWLASCSVDNTVVIWNAVKFPEILATLRGHSGLVKGL
TWDPVGKYIASQADDRSLKVWRTLDWQLETSITKPFDECGGTTHVLRLSWSPDGHYLVSA
HAMNNSGPTAQIIEREGWKTNMDFVGHRKAVTVVKFNPKIFKKKQKNGSSAKPSCPYCCC
AVGSKDRSLSVWLTCLKRPLVVIHELFDKSIMDISWTLNGLGILVCSMDGSVAFLDFSQD
ELGDPLSEEEKSRIHQSTYGKSLAIMTEAQLSTAVIENPEMLKYQ*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: kanamycin

Bacterial Selection Condition: 50ug/mL kanamycin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.

Protein Expression Results:

ProteinExpressed: Not_Applicable

ProteinPurified: Not_Applicable

SolubleProtein: Not_Applicable

Find experimental protocols in 421438

Recommended expression in:

Not Applicable

Vector Name:

pSpeedET

Synonyms:

pSpeed-ET

Sequencing Primer:

Forward: pBAD forward

Reverse: alt_bad_rev

Description:

Bacterial expression vector, arabinose or T7 induced expression, adds N-terminal MGSDKIHHHHHHENLYFQG tag; kanamycin resistance; PIPE cloning.

Comments:

If this vector contains an insert, the ccdB death cassette will not be present.

Size (bp):

5464

Parent Vector:

None

Properties:

PIPE cloning, bacterial expression, in vitro transcription, inducible expression, with tag/fusion/marker

Author Name:

Joint Center for Structural Genomics

Publications:

PMID: 18004753

Title: Combining the polymerase incomplete primer extension method for cloning and mutagenesis with microscreening to accelerate structural genomics efforts.

Vector Map:

Vector Sequence:

Sequence:

AAGAAACCAATTGTCCATATTGCATCAGACATTGCCGTCACTGCGTCTTTTACTGGCTCTTCTCGCTAACCAAACCGGTAACCCCGCTTATTAAAAGCATTCTGTAACAAAGCGGGACCAAAGCCATGACAAAAACGCGTAACAAAAGTGTCTATAATCACGGCAGAAAAGTCCACATTGATTATTTGCACGGCGTCACACTTTGCTATGCCATAGCATTTTTATCCATAAGATTAGCGGATCCTACCTGACGCTTTTTATCGCAACTCTCTACTGTTTCTCCATACCCGTAATACGACTCACTATAGGGTTTTGTTTAACTTTAAGAAGGAGATATACATACCCATGGGATCTGATAAAATTCATCATCATCATCATCACGAAAACCTGTACTTCCAGGGCGCCGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATAATGTGTGGATTTTGAGTTAGGATCCGGCGAGATTTTCAGGAGCTAAGGAAGCTAAAATGGAGAAAAAAATCACTGGATATACCACCGTTGATATATCCCAATGGCATCGTAAAGAACATTTTGAGGCATTTCAGTCAGTTGCTCAATGTACCTATAACCAGACCGTTCAGCTGGATATTACGGCCTTTTTAAAGACCGTAAAGAAAAATAAGCACAAGTTTTATCCGGCCTTTATTCACATTCTTGCCCGCCTGATGAATGCTCATCCGGAATTCCGTATGGCAATGAAAGACGGTGAGCTGGTGATATGGGATAGTGTTCACCCTTGTTACACCGTTTTCCATGAGCAAACTGAAACGTTTTCATCGCTCTGGAGTGAATACCACGACGATTTCCGGCAGTTTCTACACATATATTCGCAAGATGTGGCGTGTTACGGTGAAAACCTGGCCTATTTCCCTAAAGGGTTTATTGAGAATATGTTTTTCGTCTCAGCCAATCCCTGGGTGAGTTTCACCAGTTTTGATTTAAACGTGGCCAATATGGACAACTTCTTCGCCCCCGTTTTCACCATGGGCAAATATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGGCGATTCAGGTTCATCATGCCGTTTGTGATGGCTTCCATGTCGGCAGAATGCTTAATGAATTACAACAGTACTGCGATGAGTGGCAGGGCGGGGCGTAAACGCGTGGATCCGGCTTACTAAAAGCCAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGAATATATACTGATATGTATACCCGAAGTATGTCAAAAAGAGGTGTGCTATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCAATATCTCCGGTCTGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGGCTGGTGAAATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACACGCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGGTGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTGGCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAACTTAATTAACTCTAGATAACTGAGAAGGAGATATACATACCCGGGTAAGTTTAAACGGTCTCCAGCTTGGCTGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTTTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGCCATATTCAACGGGAAACGTCTTGCTCTAGGCCGCGATTAAATTCCAACATGGATGCTGATTTATATGGGTATAAATGGGCTCGCGATAATGTCGGGCAATCAGGTGCGACAATCTATCGATTGTATGGGAAGCCCGATGCGCCAGAGTTGTTTCTGAAACATGGCAAAGGTAGCGTTGCCAATGATGTTACAGATGAGATGGTCAGACTAAACTGGCTGACGGAATTTATGCCTCTTCCGACCATCAAGCATTTTATCCGTACTCCTGATGATGCATGGTTACTCACCACTGCGATCCCCGGGAAAACAGCATTCCAGGTATTAGAAGAATATCCTGATTCAGGTGAAAATATTGTTGATGCGCTGGCAGTGTTCCTGCGCCGGTTGCATTCGATTCCTGTTTGTAATTGTCCTTTTAACAGCGATCGCGTATTTCGTCTCGCTCAGGCGCAATCACGAATGAATAACGGTTTGGTTGATGCGAGTGATTTTGATGACGAGCGTAATGGCTGGCCTGTTGAACAAGTCTGGAAAGAAATGCATAAACTTTTGCCATTCTCACCGGATTCAGTCGTCACTCATGGTGATTTCTCACTTGATAACCTTATTTTTGACGAGGGGAAATTAATAGGTTGTATTGATGTTGGACGAGTCGGAATCGCAGACCGATACCAGGATCTTGCCATCCTATGGAACTGCCTCGGTGAGTTTTCTCCTTCATTACAGAAACGGCTTTTTCAAAAATATGGTATTGATAATCCTGATATGAATAAATTGCAGTTTCATTTGATGCTCGATGAGTTTTTCTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATACACTCCGCTATCGCTACGTGACTGGGTCATGGCTGCGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGGCAGCAGATCAATTCGCGCGCGAAGGCGAAGCGGCATGCATAATGTGCCTGTCAAATGGACGAAGCAGGGATTCTGCAAACCCTATGCTACTCCGTCAAGCCGTCAATTGTCTGATTCGTTACCAATTATGACAACTTGACGGCTACATCATTCACTTTTTCTTCACAACCGGCACGGAACTCGCTCGGGCTGGCCCCGGTGCATTTTTTAAATACCCGCGAGAAATAGAGTTGATCGTCAAAACCAACATTGCGACCGACGGTGGCGATAGGCATCCGGGTGGTGCTCAAAAGCAGCTTCGCCTGGCTGATACGTTGGTCCTCGCGCCAGCTTAAGACGCTAATCCCTAACTGCTGGCGGAAAAGATGTGACAGACGCGACGGCGACAAGCAAACATGCTGTGCGACGCTGGCGATATCAAAATTGCTGTCTGCCAGGTGATCGCTGATGTACTGACAAGCCTCGCGTACCCGATTATCCATCGGTGGATGGAGCGACTCGTTAATCGCTTCCATGCGCCGCAGTAACAATTGCTCAAGCAGATTTATCGCCAGCAGCTCCGAATAGCGCCCTTCCCCTTGCCCGGCGTTAATGATTTGCCCAAACAGGTCGCTGAAATGCGGCTGGTGCGCTTCATCCGGGCGAAAGAACCCCGTATTGGCAAATATTGACGGCCAGTTAAGCCATTCATGCCAGTAGGCGCGCGGACGAAAGTAAACCCACTGGTGATACCATTCGCGAGCCTCCGGATGACGACCGTAGTGATGAATCTCTCCTGGCGGGAACAGCAAAATATCACCCGGTCGGCAAACAAATTCTCGTCCCTGATTTTTCACCACCCCCTGACCGCGAATGGTGAGATTGAGAATATAACCTTTCATTCCCAGCGGTCGGTCGATAAAAAAATCGAGATAACCGTTGGCCTCAATCGGCGTTAAACCCGCCACCAGATGGGCATTAAACGAGTATCCCGGCAGCAGGGGATCATTTTGCGCTTCAGCCATACTTTTCATACTCCCGCCATTCAGAG

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

ColE1-type origin of replication

3306

3988

inducible trxn activator protein gene

araC

araC coding sequence

4563

5438

negative selection marker

ccdB

ccdB death cassette gene (lost in with-insert form)

1596

1805

promoter

T7 promoter

290

310

promoter

araC

araC promoter

256

285

protease cleavage site

TEV

TEV protease cleavage site

382

402

selectable marker

CmR

chloramphenicol resistance gene (CmR) (lost in with-insert form)

502

1158

selectable marker

KanR

kanamycin resistance gene

2396

3208

tag

His

N-terminal 6xHis tag

364

381

trxn regulatory element

arabinose O1 operator

161

182

trxn regulatory element

arabinose O2 operator

trxn termination sequence

rrnB

rrnB T2 transcription terminator

2258

2284

Gene

Gene Symbol :

HIRA

Symbol Nomenclature :

HIRA

SYNONYM :

DGCR1; TUP1; TUPLE1

Designation :

DiGeorge critical region gene 1|HIR histone cell cycle regulation defective homolog A|TUP1-like enhancer of split protein 1|protein HIRA

Full Nomenclature :

histone cell cycle regulator

GENEID :

7290

GI :

24981056

GenBank Accession :

BC039835

HGNC :

4916

MIM :

600237

Vega :

OTTHUMG00000150134

Target GenBank:

BC039835

Reference Sequence Alignment

  

HsCD00545876      CTGATAAAATTCATCATCATCATCATCACGAAAACCTGTACTTCCAGGGCATGAAGCTCC
NM_003325.3       --------------------------------------------------ATGAAGCTCC
                                                                    **********

HsCD00545876      TGAAGCCGACCTGGGTCAACCACAATGGCAAGCCGATTTTTTCAGTTGATATTCACCCTG
NM_003325.3       TGAAGCCGACCTGGGTCAACCACAATGGCAAGCCGATTTTTTCAGTTGATATTCACCCTG
                  ************************************************************

HsCD00545876      ACGGGACCAAGTTCGCAACTGGAGGACAAGGGCAGGATTCTGGGAAGGTTGTGATCTGGA
NM_003325.3       ACGGGACCAAGTTCGCAACTGGAGGACAAGGGCAGGATTCTGGGAAGGTTGTGATCTGGA
                  ************************************************************

HsCD00545876      ATATGTCTCCAGTCCTCCAGGAGGATGACGAGAAGGATGAAAATATTCCCAAGATGCTTT
NM_003325.3       ATATGTCTCCAGTCCTCCAGGAGGATGACGAGAAGGATGAAAATATTCCCAAGATGCTTT
                  ************************************************************

HsCD00545876      GCCAGATGGACAATCACTTAGCATGTGTGAACTGTGTGCGGTGGTCAAACAGTGGGATGT
NM_003325.3       GCCAGATGGACAATCACTTAGCATGTGTGAACTGTGTGCGGTGGTCAAACAGTGGGATGT
                  ************************************************************

HsCD00545876      ATTTAGCTTCTGGGGGAGATGACAAACTGATTATGGTGTGGAAGCGGGCTACGTACATCG
NM_003325.3       ATTTAGCTTCTGGGGGAGATGACAAACTGATTATGGTGTGGAAGCGGGCTACGTACATCG
                  ************************************************************

HsCD00545876      GCCCCAGCACCGTGTTCGGCTCCAGTGGTAAGCTTGCCAATGTGGAGCAGTGGCGGTGTG
NM_003325.3       GCCCCAGCACCGTGTTCGGCTCCAGTGGTAAGCTTGCCAATGTGGAGCAGTGGCGGTGTG
                  ************************************************************

HsCD00545876      TCTCTATCCTCCGGAATCATTCAGGCGATGTGATGGATGTAGCATGGTCTCCCCACGATG
NM_003325.3       TCTCTATCCTCCGGAATCATTCAGGCGATGTGATGGATGTAGCATGGTCTCCCCACGATG
                  ************************************************************

HsCD00545876      CCTGGCTAGCCTCATGCAGCGTGGATAACACTGTCGTCATCTGGAATGCTGTAAAGTTCC
NM_003325.3       CCTGGCTAGCCTCATGCAGCGTGGATAACACTGTCGTCATCTGGAATGCTGTAAAGTTCC
                  ************************************************************

HsCD00545876      CAGAAATTCTAGCTACTCTGAGAGGTCATTCTGGCTTGGTCAAAGGGTTGACATGGGACC
NM_003325.3       CAGAAATTCTAGCTACTCTGAGAGGTCATTCTGGCTTGGTCAAAGGGTTGACATGGGACC
                  ************************************************************

HsCD00545876      CTGTTGGTAAATACATAGCTTCTCAAGCTGATGACCGCAGCCTAAAGGTGTGGAGGACGC
NM_003325.3       CTGTTGGTAAATACATAGCTTCTCAAGCTGATGACCGCAGCCTAAAGGTGTGGAGGACGC
                  ************************************************************

HsCD00545876      TGGACTGGCAGTTGGAGACCAGCATCACCAAGCCTTTTGATGAGTGTGGAGGAACGACCC
NM_003325.3       TGGACTGGCAGTTGGAGACCAGCATCACCAAGCCTTTTGATGAGTGTGGAGGAACGACCC
                  ************************************************************

HsCD00545876      ATGTGTTGCGGCTCAGCTGGTCACCTGATGGGCATTACCTGGTGTCTGCCCATGCCATGA
NM_003325.3       ATGTGTTGCGGCTCAGCTGGTCACCTGATGGGCATTACCTGGTGTCTGCCCATGCCATGA
                  ************************************************************

HsCD00545876      ACAACTCAGGCCCCACTGCCCAGATCATCGAACGGGAGGGATGGAAGACCAACATGGACT
NM_003325.3       ACAACTCAGGCCCCACTGCCCAGATCATCGAACGGGAGGGATGGAAGACCAACATGGACT
                  ************************************************************

HsCD00545876      TTGTTGGGCACCGGAAAGCTGTGACTGTCGTGAAATTCAACCCAAAAATCTTCAAAAAGA
NM_003325.3       TTGTTGGGCACCGGAAAGCTGTGACTGTCGTGAAATTCAACCCAAAAATCTTCAAAAAGA
                  ************************************************************

HsCD00545876      AGCAGAAGAATGGGAGTTCTGCGAAGCCTAGCTGCCCGTACTGCTGCTGTGCTGTTGGCA
NM_003325.3       AGCAGAAGAATGGGAGTTCTGCGAAGCCTAGCTGCCCGTACTGCTGCTGTGCTGTTGGCA
                  ************************************************************

HsCD00545876      GCAAGGACCGCTCGCTTTCTGTCTGGCTCACATGTCTGAAACGGCCGCTGGTGGTCATCC
NM_003325.3       GCAAGGACCGCTCGCTTTCTGTCTGGCTCACATGTCTGAAACGGCCGCTGGTGGTCATCC
                  ************************************************************

HsCD00545876      ATGAACTGTTTGACAAATCCATCATGGATATTTCCTGGACTCTGAATGGGCTGGGCATCT
NM_003325.3       ATGAACTGTTTGACAAATCCATCATGGATATTTCCTGGACTCTGAATGGGCTGGGCATCT
                  ************************************************************

HsCD00545876      TGGTATGCTCTATGGACGGCTCTGTGGCATTCCTCGACTTCTCCCAGGATGAGCTTGGCG
NM_003325.3       TGGTATGCTCTATGGACGGCTCTGTGGCATTCCTCGACTTCTCCCAGGATGAGCTTGGCG
                  ************************************************************

HsCD00545876      ATCCCCTGAGCGAGGAGGAGAAGAGCCGCATTCACCAGTCCACCTATGGCAAGAGCCTAG
NM_003325.3       ATCCCCTGAGCGAGGAGGAGAAGAGCCGCATTCACCAGTCCACCTATGGCAAGAGCCTAG
                  ************************************************************

HsCD00545876      CCATCATGACCGAGGCCCAGCTCTCCACAGCCGTCATTGAGAACCCTGAGATGCTCAAGT
NM_003325.3       CCATCATGACCGAGGCCCAGCTCTCCACAGCCGTCATTGAGAACCCTGAGATGCTCAAGT
                  ************************************************************

HsCD00545876      ACCAGTAACGCGACTTAATTAACTCGTTTAAACGGTCTCCAGCTTGGCTGTTTTGGCGG-
NM_003325.3       ACCAGCGAAGGCAGCAGCAGCAGCAGCTGGACCAGAAGAGTGCTGCGACCAGGGAGATGG
                  ***** .*.*  *  :..: .*  .* * .*.*.*:. . :***  *.  :   .*. * 

HsCD00545876      ------------------------------------------------------------
NM_003325.3       GCTCAGCCACCTCAGTCGCAGGCGTTGTCAACGGGGAGAGTCTTGAAGATATCAGGAAGA
                                                                              

HsCD00545876      ------------------------------------------------------------
NM_003325.3       ATCTTTTGAAGAAACAAGTTGAGACTCGGACAGCAGATGGCCGGAGAAGAATCACGCCTC
                                                                              

HsCD00545876      ------------------------------------------------------------
NM_003325.3       TCTGCATAGCACAGCTGGACACTGGGGACTTCTCCACGGCATTCTTTAACAGCATCCCCC
                                                                              

HsCD00545876      ------------------------------------------------------------
NM_003325.3       TCTCGGGCTCCCTGGCGGGCACCATGCTCTCTTCTCATAGCAGTCCACAGCTACTGCCAC
                                                                              

HsCD00545876      ------------------------------------------------------------
NM_003325.3       TGGACTCCAGTACCCCTAACTCCTTCGGCGCCTCGAAGCCTTGCACAGAGCCTGTGGTGG
                                                                              

HsCD00545876      ------------------------------------------------------------
NM_003325.3       CTGCCAGTGCCAGACCTGCAGGCGATTCTGTCAATAAAGACAGTATGAATGCTACCTCTA
                                                                              

HsCD00545876      ------------------------------------------------------------
NM_003325.3       CTCCTGCTGCATTGTCACCTTCTGTGTTAACGACCCCGTCCAAGATCGAACCCATGAAAG
                                                                              

HsCD00545876      ------------------------------------------------------------
NM_003325.3       CGTTTGACTCCCGGTTCACAGAGCGGTCCAAAGCCACACCAGGTGCTCCTGCCCTGACCA
                                                                              

HsCD00545876      ------------------------------------------------------------
NM_003325.3       GCATGACTCCGACAGCTGTGGAAAGGTTAAAAGAGCAGAACCTTGTGAAAGAGCTGAGGC
                                                                              

HsCD00545876      ------------------------------------------------------------
NM_003325.3       CCCGAGACCTCCTGGAGAGCAGCAGTGACAGCGATGAGAAAGTCCCTTTGGCTAAGGCTT
                                                                              

HsCD00545876      ------------------------------------------------------------
NM_003325.3       CCTCACTGTCCAAGCGAAAACTTGAGCTTGAGGTAGAGACAGTAGAGAAGAAGAAGAAAG
                                                                              

HsCD00545876      ------------------------------------------------------------
NM_003325.3       GGCGGCCTCGGAAGGACTCTCGTCTCATGCCTGTGTCTCTGTCTGTCCAGTCTCCAGCTG
                                                                              

HsCD00545876      ------------------------------------------------------------
NM_003325.3       CCCTAACCGCAGAGAAGGAGGCCATGTGTCTGTCTGCACCAGCACTTGCACTGAAGCTGC
                                                                              

HsCD00545876      ------------------------------------------------------------
NM_003325.3       CAATTCCAAGCCCCCAGAGAGCATTCACCCTCCAGGTCAGCTCCGATCCTTCCATGTACA
                                                                              

HsCD00545876      ------------------------------------------------------------
NM_003325.3       TTGAGGTGGAGAATGAAGTGACAGTGGTGGGGGGCGTGAAGCTGAGCCGCCTGAAGTGCA
                                                                              

HsCD00545876      ------------------------------------------------------------
NM_003325.3       ACCGGGAAGGGAAGGAGTGGGAGACGGTACTCACCAGCCGGATCCTCACTGCTGCGGGCA
                                                                              

HsCD00545876      ------------------------------------------------------------
NM_003325.3       GCTGTGACGTGGTGTGTGTCGCCTGTGAAAAAAGGATGCTGTCAGTGTTCTCCACCTGTG
                                                                              

HsCD00545876      ------------------------------------------------------------
NM_003325.3       GTCGCCGTCTCCTCTCTCCCATCCTCCTGCCATCCCCGATCTCTACTTTGCATTGCACAG
                                                                              

HsCD00545876      ------------------------------------------------------------
NM_003325.3       GCTCCTACGTCATGGCGCTCACCGCTGCAGCCACACTCTCTGTCTGGGATGTTCACAGAC
                                                                              

HsCD00545876      ------------------------------------------------------------
NM_003325.3       AGGTGGTTGTGGTGAAAGAAGAGTCTCTACACTCCATCCTGGCAGGAAGTGATATGACGG
                                                                              

HsCD00545876      ------------------------------------------------------------
NM_003325.3       TATCACAGATCTTGCTGACGCAGCATGGAATCCCAGTAATGAACCTGTCCGATGGGAAGG
                                                                              

HsCD00545876      ------------------------------------------------------------
NM_003325.3       CGTACTGCTTTAATCCGTCACTTTCCACATGGAACCTGGTTTCTGACAAGCAGGACTCAC
                                                                              

HsCD00545876      ------------------------------------------------------------
NM_003325.3       TGGCTCAGTGTGCAGACTTTAGGAGCAGCCTGCCATCCCAGGACGCCATGCTGTGCTCAG
                                                                              

HsCD00545876      ------------------------------------------------------------
NM_003325.3       GACCGTTAGCCATAATCCAGGGCCGCACCTCCAACTCGGGAAGGCAGGCTGCCCGGCTCT
                                                                              

HsCD00545876      ------------------------------------------------------------
NM_003325.3       TCTCCGTGCCTCATGTGGTGCAGCAAGAGACCACCCTGGCCTACCTAGAGAACCAGGTGG
                                                                              

HsCD00545876      ------------------------------------------------------------
NM_003325.3       CAGCAGCACTCACCCTGCAGTCCAGCCACGAGTACCGCCATTGGCTCCTCGTCTACGCAC
                                                                              

HsCD00545876      ------------------------------------------------------------
NM_003325.3       GGTACCTCGTAAACGAAGGGTTTGAATACCGACTTCGAGAAATATGCAAGGACTTACTGG
                                                                              

HsCD00545876      ------------------------------------------------------------
NM_003325.3       GTCCGGTTCACTACTCCACTGGAAGCCAGTGGGAGTCAACAGTAGTGGGTCTGCGGAAGA
                                                                              

HsCD00545876      ------------------------------------------------------------
NM_003325.3       GGGAGCTGCTGAAGGAGCTGCTACCAGTCATCGGGCAGAACCTCCGATTCCAGCGCCTCT
                                                                              

HsCD00545876      --------------------------------------------
NM_003325.3       TCACCGAGTGTCAGGAACAGCTCGACATCCTGAGGGACAAGTAG