BF0299 (B. fragilis NCTC 9343 )
In pSpeedET (bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  BF0299
Gene Name:  hypothetical protein
Original Clone ID:
76202
Keyword:
partial cds
Species:
Bacteroides fragilis NCTC 9343
Type:
cDNA
Vector Name:
pSpeedET
Format: 
CLOSED
Source:
Joint Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI:Biology
Joint Center for Structural Genomics
BfCD00547539
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1383nts
Open reading frame: 51 to 1433

CTGATAAAATTCATCATCATCATCATCACGAAAACCTGTACTTCCAGGGCGAAGGTATGT
TGTCGCATTATACTTGTGTGGCAGATGCTATTCAGAAAGACAACCGTCCGGAACCCGCTA
AGCGTCTGTTCCGTTCGCAGGCTGTAGAAAACGAAATCATACGTGTACAGAAACTGTTGC
GTAACTCAAAGCTGGCCTGGATGTTTACCAATTGTTTCCCCAATACACTGGATACCACCG
TACACTTCCGCAAAGGCAAAGACGGCAAACCCGATACTTTTGTATATACAGGAGATATTC
ATGCCATGTGGCTCCGTGACTCGGGGGCCCAGGTATGGCCTTATGTACAACTGGCCAATT
CCGATCCGGAACTGAAAACGATGCTTGCCGGAGTTATCAACCGCCAGTTTAAATGTATCA
ATATCGATCCGTATGCCAATGCGTTCAATGATGGTCCTAAAGGGGGTGAATGGATGAGCG
ACCTGACGGATATGAAACCTGAGTTGCATGAACGCAAATGGGAGATCGACTCGCTTTGCT
ATCCGTTGCGCCTGGCTTATCAGTACTGGAAGACAACAGGGGATGCCAGTATCTTCGATG
AAGAATGGATACAGGCAATCACCAACATATTGCGTACTTTTAAGGAACAACAGCGCAAAG
ACGGTGTGGGTCCGTATAAGTTCCAACGTAAGACAGAGCGTGCTCTCGATACAGTGACCA
ACGACGGACTGGGTAATCCGGTGAAACCTGTCGGACTGATTGTTTCCACTTTCCGTCCTT
CGGACGATGCCACGACATTGCAGTATCTGGTTCCGTCCAACTTCTTTGCCGTATCTTCAC
TCCGCAAGGCAGCCGAGATACTAACAACCGTGAATAAAAAGACGGCTTTGGCCAATGAAT
GCAAGGCTTTGGCAAACGAGGTGGAAACAGCCCTGAAGAAATATGCCGTTTACAATCATC
CCAAATACGGAAAGATTTATGCTTTCGAGGTGGACGGTTTTGGTAACCACATGCTGATGG
ACGATGCCAACGTTCCGAGCCTGCTGGCAATGCCTTATCTGGGTGATGTGTCGATTGATG
ATCCGATTTATCAGAATACCCGCCGTTTTGTATGGAGCCTCGACAATCCTTACTTCTTCA
AAGGTAAGGCAGGCGAGGGCATTGGCGGACCACACATCGGATACGATATGGTATGGCCCA
TGAGTATCATGATGAAAGCTTTCACCAGCAAGGATGATGCGGAGATCAAGTCGTGCATCG
AGATGCTGATGAATACGGATGCAGGTACAGGCTTCATGCACGAGTCTTTCCATAAAGACA
ATCCTGAGAAATTTACCCGTGCCTGGTTTGCATGGCAGAATACTTTGTTCGGTGAGTTGA
TCCTGAAACTGGTGAATGAAGGTAAAGTGGATATGCTGAATAGTATACAGTAACGCGACT
TAATTAATTTGGCGGATGAGAGAAGATT

Protein sequence predicted based on nucleotide sequence

EGMLSHYTCVADAIQKDNRPEPAKRLFRSQAVENEIIRVQKLLRNSKLAWMFTNCFPNTL
DTTVHFRKGKDGKPDTFVYTGDIHAMWLRDSGAQVWPYVQLANSDPELKTMLAGVINRQF
KCINIDPYANAFNDGPKGGEWMSDLTDMKPELHERKWEIDSLCYPLRLAYQYWKTTGDAS
IFDEEWIQAITNILRTFKEQQRKDGVGPYKFQRKTERALDTVTNDGLGNPVKPVGLIVST
FRPSDDATTLQYLVPSNFFAVSSLRKAAEILTTVNKKTALANECKALANEVETALKKYAV
YNHPKYGKIYAFEVDGFGNHMLMDDANVPSLLAMPYLGDVSIDDPIYQNTRRFVWSLDNP
YFFKGKAGEGIGGPHIGYDMVWPMSIMMKAFTSKDDAEIKSCIEMLMNTDAGTGFMHESF
HKDNPEKFTRAWFAWQNTLFGELILKLVNEGKVDMLNSIQ*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
ATGGGATCTGATAAAATTCATCATCATCATCATCACGAAAACCTGTACTTCCAGGGC
3' Linker Sequence:
TAA

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Not_Applicable
ProteinPurified: Not_Applicable
SolubleProtein: Not_Applicable
Find experimental protocols in 419364
Recommended expression in:
Not Applicable