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CAPN11 (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  CAPN11
Gene Name:  calpain 11
Sequence              Map: pDNR-Dual_with_human_insert.pdf
Original Clone ID: FLH119859.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDNR-Dual               Format:  CLOSED
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: PMID: 16512675
Title: Functional proteomics approach to investigate the biological activities of cDNAs implicated in breast cancer.
Authors: HIP
HsCD00005677

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 2109nts         Open reading frame : 1 to 2109
Reference Sequence Alignment

ATGGTGGCTCACATAAACAACAGCCGGCTCAAGGCCAAGGGCGTGGGCCAGCACGACAAC
GCCCAGAACTTTGGTAACCAGAGCTTTGAGGAGCTGCGAGCAGCCTGTCTAAGAAAGGGG
GAGCTCTTCGAGGACCCCTTATTCCCTGCTGAACCCAGCTCACTGGGCTTCAAGGACCTG
GGCCCCAACTCCAAAAATGTGCAGAACATCTCCTGGCAGCGGCCCAAGGATATCATAAAC
AACCCTCTATTCATCATGGATGGGATTTCTCCAACAGACATCTGCCAGGGGATCCTCGGG
GACTGCTGGCTGCTGGCTGCCATCGGCTCCCTTACCACCTGCCCCAAACTGCTATACCGC
GTGGTGCCCAGAGGACAGAGCTTCAAGAAAAACTATGCTGGCATCTTCCATTTTCAGATT
TGGCAGTTTGGACAGTGGGTGAACGTGGTGGTAGATGACCGGCTGCCCACAAAGAATGAC
AAGCTGGTGTTTGTGCACTCAACCGAACGCAGTGAGTTCTGGAGTGCCCTGCTGGAGAAG
GCGTATGCCAAGCTGAGTGGGTCCTATGAAGCATTGTCAGGGGGCAGTACCATGGAGGGC
CTTGAGGACTTCACAGGAGGCGTGGCCCAGAGCTTCCAACTCCAGAGGCCCCCTCAGAAC
CTGCTCAGGCTCCTTAGGAAGGCCGTGGAGCGATCCTCCCTCATGGGTTGCTCCATTGAA
GTCACCAGTGATAGTGAACTGGAATCCATGACTGACAAGATGCTGGTGAGAGGGCACGCT
TACTCTGTGACTGGCCTTCAGGATGTCCACTACAGAGGCAAAATGGAAACACTGATTCGG
GTCCGGAATCCCTGGGGCCGGATTGAGTGGAATGGAGCTTGGAGTGACAGTGCCAGGGAG
TGGGAAGAGGTGGCCTCAGACATCCAGATGCAGCTGCTGCACAAGACGGAGGACGGGGAG
TTCTGGATGTCCTACCAAGATTTCCTGAACAACTTCACGCTCCTGGAGATCTGCAACCTC
ACGCCTGATACACTCTCTGGGGACTACAAGAGCTACTGGCACACCACCTTCTACGAGGGC
AGCTGGCGCAGAGGCAGCTCCGCAGGGGGCTGCAGGAACCACCCTGGCACGTTCTGGACC
AACCCCCAGTTTAAGATCTCTCTTCCTGAGGGGGATGACCCAGAGGATGACGCAGAGGGC
AATGTTGTGGCCTGCACCTGCCTGGTGGCCCTAATGCAGAAGAACTGGCGGCATGCACGG
CAGCAGGGAGCCCAGCTGCAGACCATTGGCTTTGTCCTCTACGCGGTCCCAAAAGAGTTT
CAGAACATTCAGGATGTCCACTTGAAGAAGGAATTCTTCACGAAGTATCAGGACCACGGC
TTCTCAGAGATCTTCACCAACTCACGGGAGGTGAGCAGCCAACTCCGGCTGCCTCCGGGG
GAATATATCATTATTCCCTCCACCTTTGAGCCACACAGAGATGCTGACTTCCTGCTTCGG
GTCTTCACCGAGAAGCACAGCGAGTCATGGGAATTGGATGAAGTCAACTATGCTGAGCAA
CTCCAAGAGGAAAAGGTCTCTGAGGATGACATGGACCAGGACTTCCTACATTTGTTTAAG
ATAGTGGCAGGAGAGGGCAAGGAGATAGGGGTGTATGAGCTCCAGAGGCTGCTCAACAGG
ATGGCCATCAAATTCAAAAGCTTCAAGACCAAGGGCTTTGGCCTGGATGCTTGCCGCTGC
ATGATCAACCTCATGGATAAAGATGGCTCTGGCAAGCTGGGGCTTCTAGAGTTCAAGATC
CTGTGGAAAAAACTCAAGAAATGGATGGACATCTTCAGAGAGTGTGACCAGGACCATTCA
GGCACCTTGAACTCCTATGAGATGCGCCTGGTTATTGAGAAAGCAGGCATCAAGCTGAAC
AACAAGGTAATGCAGGTCCTGGTGGCCAGGTATGCAGATGATGACCTGATCATAGACTTT
GACAGCTTCATCAGCTGTTTCCTGAGGCTAAAGACCATGTTCACATTCTTTCTAACCATG
GACCCCAAGAATACTGGCCATATTTGCTTGAGCCTGGAACAGTGGCTGCAGATGACCATG
TGGGGATAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 2229
Start on reference sequence 121
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDNR-Dual

pDNR-Dual in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  Unknown
Description: Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from).
Comments: The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp): 4938
Parent Vector: None
Empty Vector: None
Properties: Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name: BD Biosciences Clontech
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS restriction enzyme-type multiple cloning site 45 129
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4111 4574
negative selection marker SacB SacB negative selection marker gene (sucrose sensitivity) 1057 2972
primer site M13 primer M13 primer sequencing site 4839 4855
primer site T7 primer T7 RNA Pol primer sequencing site 4865 4883
recombination site loxP site loxP recombination site 1015 1048
recombination site loxP site loxP recombination site 9 42
selectable marker CmR chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) 986 327
selectable marker AmpR ampicillin resistance gene 3103 3963
splice donor site SD site splice donor site 132 136
tag HN 6xHN epitope tag 141 176

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : CAPN11
Symbol Nomenclature : CAPN11
SYNONYM : calpain11
Designation : CANP 11|calcium-activated neutral proteinase 11|calpain-11
Full Nomenclature : calpain 11
GENEID : 11131
GI : 61364130
GenBank Accession : AY890553
HGNC : 1478
MIM : 604822
Vega : OTTHUMG00000014758
Target GenBank: BC033733

 

Reference Sequence Alignment

  

HsCD00005677      ------------------------------------------------------------
NM_007058.3       ATGCTGTACTCCCCAGGGCCGAGTCTTCCGGAGTCAGCAGAGAGCCTGGATGGATCACAG
                                                                              

HsCD00005677      ---------------------------------------------------ATGGTGGCT
NM_007058.3       GAGGATAAGCCTCGGGGCTCATGTGCGGAGCCCACTTTTACTGATACGGGAATGGTGGCT
                                                                     *********

HsCD00005677      CACATAAACAACAGCCGGCTCAAGGCCAAGGGCGTGGGCCAGCACGACAACGCCCAGAAC
NM_007058.3       CACATAAACAACAGCCGGCTCAAGGCCAAGGGCGTGGGCCAGCACGACAACGCCCAGAAC
                  ************************************************************

HsCD00005677      TTTGGTAACCAGAGCTTTGAGGAGCTGCGAGCAGCCTGTCTAAGAAAGGGGGAGCTCTTC
NM_007058.3       TTTGGTAACCAGAGCTTTGAGGAGCTGCGAGCAGCCTGTCTAAGAAAGGGGGAGCTCTTC
                  ************************************************************

HsCD00005677      GAGGACCCCTTATTCCCTGCTGAACCCAGCTCACTGGGCTTCAAGGACCTGGGCCCCAAC
NM_007058.3       GAGGACCCCTTATTCCCTGCTGAACCCAGCTCACTGGGCTTCAAGGACCTGGGCCCCAAC
                  ************************************************************

HsCD00005677      TCCAAAAATGTGCAGAACATCTCCTGGCAGCGGCCCAAGGATATCATAAACAACCCTCTA
NM_007058.3       TCCAAAAATGTGCAGAACATCTCCTGGCAGCGGCCCAAGGATATCATAAACAACCCTCTA
                  ************************************************************

HsCD00005677      TTCATCATGGATGGGATTTCTCCAACAGACATCTGCCAGGGGATCCTCGGGGACTGCTGG
NM_007058.3       TTCATCATGGATGGGATTTCTCCAACAGACATCTGCCAGGGGATCCTCGGGGACTGCTGG
                  ************************************************************

HsCD00005677      CTGCTGGCTGCCATCGGCTCCCTTACCACCTGCCCCAAACTGCTATACCGCGTGGTGCCC
NM_007058.3       CTGCTGGCTGCCATCGGCTCCCTTACCACCTGCCCCAAACTGCTATACCGCGTGGTGCCC
                  ************************************************************

HsCD00005677      AGAGGACAGAGCTTCAAGAAAAACTATGCTGGCATCTTCCATTTTCAGATTTGGCAGTTT
NM_007058.3       AGAGGACAGAGCTTCAAGAAAAACTATGCTGGCATCTTCCATTTTCAGATTTGGCAGTTT
                  ************************************************************

HsCD00005677      GGACAGTGGGTGAACGTGGTGGTAGATGACCGGCTGCCCACAAAGAATGACAAGCTGGTG
NM_007058.3       GGACAGTGGGTGAACGTGGTGGTAGATGACCGGCTGCCCACAAAGAATGACAAGCTGGTG
                  ************************************************************

HsCD00005677      TTTGTGCACTCAACCGAACGCAGTGAGTTCTGGAGTGCCCTGCTGGAGAAGGCGTATGCC
NM_007058.3       TTTGTGCACTCAACCGAACGCAGTGAGTTCTGGAGTGCCCTGCTGGAGAAGGCGTATGCC
                  ************************************************************

HsCD00005677      AAGCTGAGTGGGTCCTATGAAGCATTGTCAGGGGGCAGTACCATGGAGGGCCTTGAGGAC
NM_007058.3       AAGCTGAGTGGGTCCTATGAAGCATTGTCAGGGGGCAGTACCATGGAGGGCCTTGAGGAC
                  ************************************************************

HsCD00005677      TTCACAGGAGGCGTGGCCCAGAGCTTCCAACTCCAGAGGCCCCCTCAGAACCTGCTCAGG
NM_007058.3       TTCACAGGAGGCGTGGCCCAGAGCTTCCAACTCCAGAGGCCCCCTCAGAACCTGCTCAGG
                  ************************************************************

HsCD00005677      CTCCTTAGGAAGGCCGTGGAGCGATCCTCCCTCATGGGTTGCTCCATTGAAGTCACCAGT
NM_007058.3       CTCCTTAGGAAGGCCGTGGAGCGATCCTCCCTCATGGGTTGCTCCATTGAAGTCACCAGT
                  ************************************************************

HsCD00005677      GATAGTGAACTGGAATCCATGACTGACAAGATGCTGGTGAGAGGGCACGCTTACTCTGTG
NM_007058.3       GATAGTGAACTGGAATCCATGACTGACAAGATGCTGGTGAGAGGGCACGCTTACTCTGTG
                  ************************************************************

HsCD00005677      ACTGGCCTTCAGGATGTCCACTACAGAGGCAAAATGGAAACACTGATTCGGGTCCGGAAT
NM_007058.3       ACTGGCCTTCAGGATGTCCACTACAGAGGCAAAATGGAAACACTGATTCGGGTCCGGAAT
                  ************************************************************

HsCD00005677      CCCTGGGGCCGGATTGAGTGGAATGGAGCTTGGAGTGACAGTGCCAGGGAGTGGGAAGAG
NM_007058.3       CCCTGGGGCCGGATTGAGTGGAATGGAGCTTGGAGTGACAGTGCCAGGGAGTGGGAAGAG
                  ************************************************************

HsCD00005677      GTGGCCTCAGACATCCAGATGCAGCTGCTGCACAAGACGGAGGACGGGGAGTTCTGGATG
NM_007058.3       GTGGCCTCAGACATCCAGATGCAGCTGCTGCACAAGACGGAGGACGGGGAGTTCTGGATG
                  ************************************************************

HsCD00005677      TCCTACCAAGATTTCCTGAACAACTTCACGCTCCTGGAGATCTGCAACCTCACGCCTGAT
NM_007058.3       TCCTACCAAGATTTCCTGAACAACTTCACGCTCCTGGAGATCTGCAACCTCACGCCTGAT
                  ************************************************************

HsCD00005677      ACACTCTCTGGGGACTACAAGAGCTACTGGCACACCACCTTCTACGAGGGCAGCTGGCGC
NM_007058.3       ACACTCTCTGGGGACTACAAGAGCTACTGGCACACCACCTTCTACGAGGGCAGCTGGCGC
                  ************************************************************

HsCD00005677      AGAGGCAGCTCCGCAGGGGGCTGCAGGAACCACCCTGGCACGTTCTGGACCAACCCCCAG
NM_007058.3       AGAGGCAGCTCCGCAGGGGGCTGCAGGAACCACCCTGGCACGTTCTGGACCAACCCCCAG
                  ************************************************************

HsCD00005677      TTTAAGATCTCTCTTCCTGAGGGGGATGACCCAGAGGATGACGCAGAGGGCAATGTTGTG
NM_007058.3       TTTAAGATCTCTCTTCCTGAGGGGGATGACCCAGAGGATGACGCAGAGGGCAATGTTGTG
                  ************************************************************

HsCD00005677      GCCTGCACCTGCCTGGTGGCCCTAATGCAGAAGAACTGGCGGCATGCACGGCAGCAGGGA
NM_007058.3       GTCTGCACCTGCCTGGTGGCCCTAATGCAGAAGAACTGGCGGCATGCACGGCAGCAGGGA
                  * **********************************************************

HsCD00005677      GCCCAGCTGCAGACCATTGGCTTTGTCCTCTACGCGGTCCCAAAAGAGTTTCAGAACATT
NM_007058.3       GCCCAGCTGCAGACCATTGGCTTTGTCCTCTACGCGGTCCCAAAAGAGTTTCAGAACATT
                  ************************************************************

HsCD00005677      CAGGATGTCCACTTGAAGAAGGAATTCTTCACGAAGTATCAGGACCACGGCTTCTCAGAG
NM_007058.3       CAGGATGTCCACTTGAAGAAGGAATTCTTCACGAAGTATCAGGACCACGGCTTCTCAGAG
                  ************************************************************

HsCD00005677      ATCTTCACCAACTCACGGGAGGTGAGCAGCCAACTCCGGCTGCCTCCGGGGGAATATATC
NM_007058.3       ATCTTCACCAACTCACGGGAGGTGAGCAGCCAACTCCGGCTGCCTCCGGGGGAATATATC
                  ************************************************************

HsCD00005677      ATTATTCCCTCCACCTTTGAGCCACACAGAGATGCTGACTTCCTGCTTCGGGTCTTCACC
NM_007058.3       ATTATTCCCTCCACCTTTGAGCCACACAGAGATGCTGACTTCCTGCTTCGGGTCTTCACC
                  ************************************************************

HsCD00005677      GAGAAGCACAGCGAGTCATGGGAATTGGATGAAGTCAACTATGCTGAGCAACTCCAAGAG
NM_007058.3       GAGAAGCACAGCGAGTCATGGGAATTGGATGAAGTCAACTATGCTGAGCAACTCCAAGAG
                  ************************************************************

HsCD00005677      GAAAAGGTCTCTGAGGATGACATGGACCAGGACTTCCTACATTTGTTTAAGATAGTGGCA
NM_007058.3       GAAAAGGTCTCTGAGGATGACATGGACCAGGACTTCCTACATTTGTTTAAGATAGTGGCA
                  ************************************************************

HsCD00005677      GGAGAGGGCAAGGAGATAGGGGTGTATGAGCTCCAGAGGCTGCTCAACAGGATGGCCATC
NM_007058.3       GGAGAGGGCAAGGAGATAGGGGTGTATGAGCTCCAGAGGCTGCTCAACAGGATGGCCATC
                  ************************************************************

HsCD00005677      AAATTCAAAAGCTTCAAGACCAAGGGCTTTGGCCTGGATGCTTGCCGCTGCATGATCAAC
NM_007058.3       AAATTCAAAAGCTTCAAGACCAAGGGCTTTGGCCTGGATGCTTGCCGCTGCATGATCAAC
                  ************************************************************

HsCD00005677      CTCATGGATAAAGATGGCTCTGGCAAGCTGGGGCTTCTAGAGTTCAAGATCCTGTGGAAA
NM_007058.3       CTCATGGATAAAGATGGCTCTGGCAAGCTGGGGCTTCTAGAGTTCAAGATCCTGTGGAAA
                  ************************************************************

HsCD00005677      AAACTCAAGAAATGGATGGACATCTTCAGAGAGTGTGACCAGGACCATTCAGGCACCTTG
NM_007058.3       AAACTCAAGAAATGGATGGACATCTTCAGAGAGTGTGACCAGGACCATTCAGGCACCTTG
                  ************************************************************

HsCD00005677      AACTCCTATGAGATGCGCCTGGTTATTGAGAAAGCAGGCATCAAGCTGAACAACAAGGTA
NM_007058.3       AACTCCTATGAGATGCGCCTGGTTATTGAGAAAGCAGGCATCAAGCTGAACAACAAGGTA
                  ************************************************************

HsCD00005677      ATGCAGGTCCTGGTGGCCAGGTATGCAGATGATGACCTGATCATAGACTTTGACAGCTTC
NM_007058.3       ATGCAGGTCCTGGTGGCCAGGTATGCAGATGATGACCTGATCATAGACTTTGACAGCTTC
                  ************************************************************

HsCD00005677      ATCAGCTGTTTCCTGAGGCTAAAGACCATGTTCACATTCTTTCTAACCATGGACCCCAAG
NM_007058.3       ATCAGCTGTTTCCTGAGGCTAAAGACCATGTTCACATTCTTTCTAACCATGGACCCCAAG
                  ************************************************************

HsCD00005677      AATACTGGCCATATTTGCTTGAGCCTGGAACAGTGGCTGCAGATGACCATGTGGGGATAG
NM_007058.3       AATACTGGCCATATTTGCTTGAGCCTGGAACAGTGGCTGCAGATGACCATGTGGGGATAG
                  ************************************************************

Pathways

Panther : Huntington disease
WikiPathway : Integrin-mediated cell adhesion

Protein

SMART domain : CysPc : Calpain-like thiol protease family.
SMART domain : calpain_III :
UniProt : Q9UMQ6
HPRD : 05315

Clone

Cloning Information : 119859
HIP Master Clone ID : 95513
Original Clone ID : FLH119859.01X

Organism


TAX_ID : 9606
Species Specific ID: 11131

Genome

Chromosome : 6
Map Location : 6p12
Ensembl : ENSG00000137225

Reagents

Labome : CAPN11-antibody