DNASU Plasmid | OTUD7B (H. sapiens )<br>In pET15_NESG (His-tagged bacterial expression vector)

Explanation of Terms

Gene:

Gene Symbol: OTUD7B

Gene Name: OTU domain containing 7B

Original Clone ID:

HR3638C-125-443-14.12

Keyword:

targeted domain

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pET15_NESG

Format:

CLOSED

Source:

Northeast Structural Genomics Consortium

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

PSI:Biology
Northeast Structural Genomics Consortium

HsCD00585611

Price:

AVAILABLE

No restriction
Special MTA: PSI/NESG

Insert Sequence
Protein Sequence

Insert sequence: 963nts

Open reading frame: 51 to 1013

Reference Sequence Alignment

TTTAAGAAGGAGATATACCATGGGGCCATCACCATCACCATCACAGCCATATGGAGCACC
CCCTGGAAATGCCCATCTGTGCCTTCCAGCTTCCAGATCTCACTGTATACAATGAAGACT
TCCGCAGCTTCATAGAGAGAGACCTCATTGAGCAGTCCATGCTGGTTGCCTTGGAACAGG
CAGGGCGTTTGAACTGGTGGGTGAGTGTGGATCCCACCTCTCAGAGGCTGCTTCCTTTGG
CAACTACTGGAGATGGGAACTGCCTCCTGCATGCAGCCTCCCTTGGAATGTGGGGTTTCC
ATGATCGGGACTTGATGCTGCGGAAAGCTTTGTATGCACTGATGGAGAAGGGAGTTGAGA
AGGAAGCGTTGAAAAGGCGCTGGAGGTGGCAGCAGACACAGCAGAATAAAGAGTCAGGGC
TGGTATACACAGAAGATGAATGGCAGAAGGAGTGGAATGAACTGATCAAGCTTGCCTCAA
GTGAACCCCGAATGCATCTAGGTACCAATGGAGCCAACTGTGGTGGGGTGGAGAGTTCTG
AGGAGCCTGTATATGAGAGCCTTGAAGAGTTTCACGTCTTTGTCCTTGCTCATGTGCTTA
GGAGGCCCATAGTCGTCGTGGCAGACACCATGCTGAGGGACTCCGGAGGGGAAGCATTTG
CCCCTATTCCCTTTGGAGGAATCTATCTGCCTTTGGAGGTCCCAGCCAGCCAGTGTCACC
GCTCCCCTCTGGTGCTCGCCTATGATCAGGCCCACTTTTCTGCACTCGTGTCCATGGAGC
AGAAGGAGAATACCAAGGAACAAGCTGTGATCCCACTTACAGATTCAGAGTATAAGCTGC
TGCCCTTGCACTTTGCTGTGGACCCTGGAAAGGGCTGGGAGTGGGGCAAAGATGATAGTG
ACAATGTCCGATTGGCCAGTGTAATTCTGTCCCTAGAGGTCAAATTGCATCTGCTGCATA
GCTACATGAATGTGAAGTGGATCCCACTGTCCTCTGATGCACAGGCTCCTTAGCTCGAGA
TCCGGCTG

Protein sequence predicted based on nucleotide sequence

MEHPLEMPICAFQLPDLTVYNEDFRSFIERDLIEQSMLVALEQAGRLNWWVSVDPTSQRL
LPLATTGDGNCLLHAASLGMWGFHDRDLMLRKALYALMEKGVEKEALKRRWRWQQTQQNK
ESGLVYTEDEWQKEWNELIKLASSEPRMHLGTNGANCGGVESSEEPVYESLEEFHVFVLA
HVLRRPIVVVADTMLRDSGGEAFAPIPFGGIYLPLEVPASQCHRSPLVLAYDQAHFSALV
SMEQKENTKEQAVIPLTDSEYKLLPLHFAVDPGKGWEWGKDDSDNVRLASVILSLEVKLH
LLHSYMNVKWIPLSSDAQAP*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

ProteinPurified: Not_Tested

SolubleProtein: Not_Tested

ProteinExpressed: Tested_Not_Found

Find experimental protocols in HR3638C

Recommended expression in:

Not Applicable

Vector Name:

pET15_NESG

Synonyms:

pET15, pET15-modified, pET15Cc, pET15_NESG

Sequencing Primer:

Forward: T7

Reverse: T7 terminator

Description:

Bacterial (E. coli) expression vector with T7 lac promoter, adds N-terminal 6x His tag; amp resistance; restriction enzyme cloning.

Comments:

Modified Novagen pET vector-common MCS

Size (bp):

5723

Parent Vector:

None

Properties:

bacterial expression, in vitro transcription, multiple cloning site, with tag/fusion/marker

Author Name:

Gaetano T. Montleione
Jessica Bearden
Northeast Structural Genomics Consortium

Publications:

PMID: 15808222

Title: Robotic cloning and Protein Production Platform of the Northeast Structural Genomics Consortium

Vector Map:

Vector Sequence:

Sequence:

TTCTCATGTTTGACAGCTTATCATCGATAAGCTAGCTTTAATGCGGTAGTTTATCACAGTTAAATTGCTAACGCAGTCAGGCACCGTGTATGAAATCTAACAATGCGCTCATCGTCATCCTCGGCACCGTCACCCTGGATGCTGTAGGCATAGGCTTGGTTATGCCGGTACTGCCGGGCCTCTTGCGGGATATCCGGATATAGTTCCTCCTTTCAGCAAAAAACCCCTCAAGACCCGTTTAGAGGCCCCAAGGGGTTATGCTAGTTATTGCTCAGCGGTGGCAGCAGCCAACTCAGCTTCCTTTCGGGCTTTGTTAGCAGCCGGATCTCGAGAAGCTTTGCGTCGACAGAGCTCGCAGGATCCGCAGAATTCGCCATATGGCTGTGATGGTGATGGTGATGGCCCATGGTATATCTCCTTCTTAAAGTTAAACAAAATTATTTCTAGAGGGGAATTGTTATCCGCTCACAATTCCCCTATAGTGAGTCGTATTAATTTCGCGGGATCGAGATCTCGATCCTCTACGCCGGACGCATCGTGGCCGGCATCACCGGCGCCACAGGTGCGGTTGCTGGCGCCTATATCGCCGACATCACCGATGGGGAAGATCGGGCTCGCCACTTCGGGCTCATGAGCGCTTGTTTCGGCGTGGGTATGGTGGCAGGCCCCGTGGCCGGGGGACTGTTGGGCGCCATCTCCTTGCATGCACCATTCCTTGCGGCGGCGGTGCTCAACGGCCTCAACCTACTACTGGGCTGCTTCCTAATGCAGGAGTCGCATAAGGGAGAGCGTCGAGATCCCGGACACCATCGAATGGCGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAATGTGAAACCAGTAACGTTATACGATGTCGCAGAGTATGCCGGTGTCTCTTATCAGACCGTTTCCCGCGTGGTGAACCAGGCCAGCCACGTTTCTGCGAAAACGCGGGAAAAAGTGGAAGCGGCGATGGCGGAGCTGAATTACATTCCCAACCGCGTGGCACAACAACTGGCGGGCAAACAGTCGTTGCTGATTGGCGTTGCCACCTCCAGTCTGGCCCTGCACGCGCCGTCGCAAATTGTCGCGGCGATTAAATCTCGCGCCGATCAACTGGGTGCCAGCGTGGTGGTGTCGATGGTAGAACGAAGCGGCGTCGAAGCCTGTAAAGCGGCGGTGCACAATCTTCTCGCGCAACGCGTCAGTGGGCTGATCATTAACTATCCGCTGGATGACCAGGATGCCATTGCTGTGGAAGCTGCCTGCACTAATGTTCCGGCGTTATTTCTTGATGTCTCTGACCAGACACCCATCAACAGTATTATTTTCTCCCATGAAGACGGTACGCGACTGGGCGTGGAGCATCTGGTCGCATTGGGTCACCAGCAAATCGCGCTGTTAGCGGGCCCATTAAGTTCTGTCTCGGCGCGTCTGCGTCTGGCTGGCTGGCATAAATATCTCACTCGCAATCAAATTCAGCCGATAGCGGAACGGGAAGGCGACTGGAGTGCCATGTCCGGTTTTCAACAAACCATGCAAATGCTGAATGAGGGCATCGTTCCCACTGCGATGCTGGTTGCCAACGATCAGATGGCGCTGGGCGCAATGCGCGCCATTACCGAGTCCGGGCTGCGCGTTGGTGCGGATATCTCGGTAGTGGGATACGACGATACCGAAGACAGCTCATGTTATATCCCGCCGTTAACCACCATCAAACAGGATTTTCGCCTGCTGGGGCAAACCAGCGTGGACCGCTTGCTGCAACTCTCTCAGGGCCAGGCGGTGAAGGGCAATCAGCTGTTGCCCGTCTCACTGGTGAAAAGAAAAACCACCCTGGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTAAGTTAGCTCACTCATTAGGCACCGGGATCTCGACCGATGCCCTTGAGAGCCTTCAACCCAGTCAGCTCCTTCCGGTGGGCGCGGGGCATGACTATCGTCGCCGCACTTATGACTGTCTTCTTTATCATGCAACTCGTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGCGCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCCTTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATGGCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTCCCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTGTCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACCAGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGCCTCGGCGAGCACATGGAACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGTCGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACGGATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAACCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCATCTCGGGCAGCGTTGGGTCCTGGCCACGGGTGCGCATGATCGTGCTCCTGTCGTTGAGGACCCGGCTAGGCTGGCGGGGTTGCCTTACTGGTTAGCAGAATGAATCACCGATACGCGAGCGAACGTGAAGCGACTGCTGCTGCAAAACGTCTGCGACCTGAGCAACAACATGAATGGTCTTCGGTTTCCGTGTTTCGTAAAGTCTGGAAACGCGGAAGTCAGCGCCCTGCACCATTATGTTCCGGATCTGCATCGCAGGATGCTGCTGGCTACCCTGTGGAACACCTACATCTGTATTAACGAAGCGCTGGCATTGACCCTGAGTGATTTTTCTCTGGTCCCGCCGCATCCATACCGCCAGTTGTTTACCCTCACAACGTTCCAGTAACCGGGCATGTTCATCATCAGTAACCCGTATCGTGAGCATCCTCTCTCGTTTCATCGGTATCATTACCCCCATGAACAGAAATCCCCCTTACACGGAGGCATCAGTGACCAAACAGGAAAAAACCGCCCTTAACATGGCCCGCTTTATCAGAAGCCAGACATTAACGCTTCTGGAGAAACTCAACGAGCTGGACGCGGATGAACAGGCAGACATCTGTGAATCGCTTCACGACCACGCTGATGAGCTTTACCGCAGCTGCCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTGCAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAACACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTTCAAGAA

Vector Features:

Type

Name

Description

Start

End

MCS

MCS (includes SphI, XbaI, EcoRI, BamHI, XhoI)

327

452

bacterial origin

ori

ColE1 origin of replication

3881

4563

gene fragment

ROP

repressor of primer ROP sequence

3281

3457

primer

T7 forward

T7 forward sequencing primer

481

496

primer

T7 reverse

T7 reverse sequencing primer

257

275

promoter

T7 promoter

478

497

repressor binding site

lacO

lacO regulatory sequence

456

478

selectable marker

AmpR

ampicillin resistance gene

4661

5230

tag

His

N-terminal 6x His tag

387

404

trxn termination sequence

T7 term

T7 termination sequence

257

275

Gene

Gene Symbol :

OTUD7B

Symbol Nomenclature :

OTUD7B

SYNONYM :

CEZANNE; ZA20D1

Designation :

Full Nomenclature :

OTU domain containing 7B

GENEID :

56957

Locus Tag :

RP11-212K13.2

GI :

118026942

GenBank Accession :

NM_020205

HGNC :

16683

MIM :

611748

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00585611        ------------------------------------------------------------
NM_020205.3         ATGACCCTGGACATGGATGCTGTTCTGTCAGATTTTGTCCGTTCCACAGGAGCAGAGCCA
XM_005277414.1      ------------------------------------------------------------
                                                                                

HsCD00585611        ------------------------------------------------------------
NM_020205.3         GGGCTAGCGCGAGATCTCCTAGAAGGAAAGAATTGGGATGTGAATGCCGCCCTCAGTGAT
XM_005277414.1      -------------------------------------------ATGCCGCCCTCAGTGAT
                                                                                

HsCD00585611        --------------------------------------------TTTAAGAAGGAGATAT
NM_020205.3         TTTGAACAGCTACGTCAAGTCCATGCTGGAAACCTACCCCCATCCTTTAGTGAGGGGAGT
XM_005277414.1      TTTGAACAGCTACGTCAAGTCCATGCTGGAAACCTACCCCCATCCTTTAGTGAGGGGAGT
                                                                 **:**:..*.*.:.*

HsCD00585611        ACCATGGGGCCATCACCATCACCATCACAGCCATATGGAGCACCCCCTGGAAATGCCCAT
NM_020205.3         GGTGGCTCCAGGACCCCTGAAAAAGGGTTTTCTGACAGAGAGCCTACTCGCCCTCCCCGA
XM_005277414.1      GGTGGCTCCAGGACCCCTGAAAAAGGGTTTTCTGACAGAGAGCCTACTCGCCCTCCCCGA
                    .  .     . .:*.**: .*..*  . :  *: * .***..** .** *...* ***.:

HsCD00585611        CTGTGCCTTCCAG-----------------------------------------------
NM_020205.3         CCCATCCTCCAGCGGCAGGATGACATCGTTCAAGAAAAACGCCTGTCTAGGGGCATCTCC
XM_005277414.1      CCCATCCTCCAGCGGCAGGATGACATCG--------------------------------
                    *  : *** *..                                                

HsCD00585611        ------------------------------------------------------------
NM_020205.3         CACGCCAGCTCCAGCATTGTTTCCCTGGCCCGGTCCCATGTCTCCTCCAATGGTGGGGGT
XM_005277414.1      ------------------------------------------------------------
                                                                                

HsCD00585611        ------------------------------------------------CTTCCAGATCTC
NM_020205.3         GGGGGGAGCAATGAGCACCCCCTGGAAATGCCCATCTGTGCCTTCCAGCTTCCAGATCTC
XM_005277414.1      ------------------------------------------TTCAAGCTTCCAGATCTC
                                                                    ************

HsCD00585611        ACTGTATACAATGAAGACTTCCGCAGCTTCATAGAGAGAGACCTCATTGAGCAGTCCATG
NM_020205.3         ACTGTATACAATGAAGACTTCCGCAGCTTCATAGAGAGAGACCTCATTGAGCAGTCCATG
XM_005277414.1      ACTGTATACAATGAAGACTTCCGCAGCTTCATAGAGAGAGACCTCATTGAGCAGTCCATG
                    ************************************************************

HsCD00585611        CTGGTTGCCTTGGAACAGGCAGGGCGTTTGAACTGGTGGGTGAGTGTGGATCCCACCTCT
NM_020205.3         CTGGTTGCCTTGGAACAGGCAGGGCGTTTGAACTGGTGGGTGAGTGTGGATCCCACCTCT
XM_005277414.1      CTGGTTGCCTTGGAACAGGCAGGGCGTTTGAACTGGTGGGTGAGTGTGGATCCCACCTCT
                    ************************************************************

HsCD00585611        CAGAGGCTGCTTCCTTTGGCAACTACTGGAGATGGGAACTGCCTCCTGCATGCAGCCTCC
NM_020205.3         CAGAGGCTGCTTCCTTTGGCAACTACTGGAGATGGGAACTGCCTCCTGCATGCAGCCTCC
XM_005277414.1      CAGAGGCTGCTTCCTTTGGCAACTACTGGAGATGGGAACTGCCTCCTGCATGCAGCCTCC
                    ************************************************************

HsCD00585611        CTTGGAATGTGGGGTTTCCATGATCGGGACTTGATGCTGCGGAAAGCTTTGTATGCACTG
NM_020205.3         CTTGGAATGTGGGGTTTCCATGATCGGGACTTGATGCTGCGGAAAGCTTTGTATGCACTG
XM_005277414.1      CTTGGAATGTGGGGTTTCCATGATCGGGACTTGATGCTGCGGAAAGCTTTGTATGCACTG
                    ************************************************************

HsCD00585611        ATGGAGAAGGGAGTTGAGAAGGAAGCGTTGAAAAGGCGCTGGAGGTGGCAGCAGACACAG
NM_020205.3         ATGGAGAAGGGAGTTGAGAAGGAAGCGTTGAAAAGGCGCTGGAGGTGGCAGCAGACACAG
XM_005277414.1      ATGGAGAAGGGAGTTGAGAAGGAAGCGTTGAAAAGGCGCTGGAGGTGGCAGCAGACACAG
                    ************************************************************

HsCD00585611        CAGAATAAAGAGTCAGGGCTGGTATACACAGAAGATGAATGGCAGAAGGAGTGGAATGAA
NM_020205.3         CAGAATAAAGAGTCAGGGCTGGTATACACAGAAGATGAATGGCAGAAGGAGTGGAATGAA
XM_005277414.1      CAGAATAAAGAGTCAGGGCTGGTATACACAGAAGATGAATGGCAGAAGGAGTGGAATGAA
                    ************************************************************

HsCD00585611        CTGATCAAGCTTGCCTCAAGTGAACCCCGAATGCATCTAGGTACCAATGGAGCCAACTGT
NM_020205.3         CTGATCAAGCTTGCCTCAAGTGAACCCCGAATGCATCTAGGTACCAATGGAGCCAACTGT
XM_005277414.1      CTGATCAAGCTTGCCTCAAGTGAACCCCGAATGCATCTAGGTACCAATGGAGCCAACTGT
                    ************************************************************

HsCD00585611        GGTGGGGTGGAGAGTTCTGAGGAGCCTGTATATGAGAGCCTTGAAGAGTTTCACGTCTTT
NM_020205.3         GGTGGGGTGGAGAGTTCTGAGGAGCCTGTATATGAGAGCCTTGAAGAGTTTCACGTCTTT
XM_005277414.1      GGTGGGGTGGAGAGTTCTGAGGAGCCTGTATATGAGAGCCTTGAAGAGTTTCACGTCTTT
                    ************************************************************

HsCD00585611        GTCCTTGCTCATGTGCTTAGGAGGCCCATAGTCGTCGTGGCAGACACCATGCTGAGGGAC
NM_020205.3         GTCCTTGCTCATGTGCTTAGGAGGCCCATAGTCGTCGTGGCAGACACCATGCTGAGGGAC
XM_005277414.1      GTCCTTGCTCATGTGCTTAGGAGGCCCATAGTCGTCGTGGCAGACACCATGCTGAGGGAC
                    ************************************************************

HsCD00585611        TCCGGAGGGGAAGCATTTGCCCCTATTCCCTTTGGAGGAATCTATCTGCCTTTGGAGGTC
NM_020205.3         TCCGGAGGGGAAGCATTTGCCCCTATTCCCTTTGGAGGAATCTATCTGCCTTTGGAGGTC
XM_005277414.1      TCCGGAGGGGAAGCATTTGCCCCTATTCCCTTTGGAGGAATCTATCTGCCTTTGGAGGTC
                    ************************************************************

HsCD00585611        CCAGCCAGCCAGTGTCACCGCTCCCCTCTGGTGCTCGCCTATGATCAGGCCCACTTTTCT
NM_020205.3         CCAGCCAGCCAGTGTCACCGCTCCCCTCTGGTGCTCGCCTATGATCAGGCCCACTTTTCT
XM_005277414.1      CCAGCCAGCCAGTGTCACCGCTCCCCTCTGGTGCTCGCCTATGATCAGGCCCACTTTTCT
                    ************************************************************

HsCD00585611        GCACTCGTGTCCATGGAGCAGAAGGAGAATACCAAGGAACAAGCTGTGATCCCACTTACA
NM_020205.3         GCACTCGTGTCCATGGAGCAGAAGGAGAATACCAAGGAACAAGCTGTGATCCCACTTACA
XM_005277414.1      GCACTCGTGTCCATGGAGCAGAAGGAGAATACCAAGGAACAAGCTGTGATCCCACTTACA
                    ************************************************************

HsCD00585611        GATTCAGAGTATAAGCTGCTGCCCTTGCACTTTGCTGTGGACCCTGGAAAGGGCTGGGAG
NM_020205.3         GATTCAGAGTATAAGCTGCTGCCCTTGCACTTTGCTGTGGACCCTGGAAAGGGCTGGGAG
XM_005277414.1      GATTCAGAGTATAAGCTGCTGCCCTTGCACTTTGCTGTGGACCCTGGAAAGGGCTGGGAG
                    ************************************************************

HsCD00585611        TGGGGCAAAGATGATAGTGACAATGTCCGATTGGCCAGTGTAATTCTGTCCCTAGAGGTC
NM_020205.3         TGGGGCAAAGATGATAGTGACAATGTCCGATTGGCCAGTGTAATTCTGTCCCTAGAGGTC
XM_005277414.1      TGGGGCAAAGATGATAGTGACAATGTCCGATTGGCCAGTGTAATTCTGTCCCTAGAGGTC
                    ************************************************************

HsCD00585611        AAATTGCATCTGCTGCATAGCTACATGAATGTGAAGTGGATCCCACTGTCCTCTGATGCA
NM_020205.3         AAATTGCATCTGCTGCATAGCTACATGAATGTGAAGTGGATCCCACTGTCCTCTGATGCA
XM_005277414.1      AAATTGCATCTGCTGCATAGCTACATGAATGTGAAGTGGATCCCACTGTCCTCTGATGCA
                    ************************************************************

HsCD00585611        CAGGCTCCTTAGCTCGAGATCCGGCTG---------------------------------
NM_020205.3         CAGGCTCCTCTGGCCCAGCCTGAGTCCCCCACCGCCTCAGCTGGAGATGAGCCCCGGTCC
XM_005277414.1      CAGGCTCCTCTGGCCCAGCCTGAGTCCCCCACCGCCTCAGCTGGAGATGAGCCCCGGTCC
                    ********* :*  * **.   .*                                    

HsCD00585611        ------------------------------------------------------------
NM_020205.3         ACTCCTGAGTCTGGAGACTCAGACAAGGAGTCAGTTGGCAGCAGTTCCACCAGCAACGAG
XM_005277414.1      ACTCCTGAGTCTGGAGACTCAGACAAGGAGTCAGTTGGCAGCAGTTCCACCAGCAACGAG
                                                                                

HsCD00585611        ------------------------------------------------------------
NM_020205.3         GGCGGCCGGCGGAAGGAGAAGTCAAAGCGAGATCGGGAGAAGGACAAGAAGAGAGCAGAT
XM_005277414.1      GGCGGCCGGCGGAAGGAGAAGTCAAAGCGAGATCGGGAGAAGGACAAGAAGAGAGCAGAT
                                                                                

HsCD00585611        ------------------------------------------------------------
NM_020205.3         TCTGTGGCTAACAAACTGGGCAGCTTTGGCAAAACCTTGGGCAGCAAGCTCAAGAAGAAC
XM_005277414.1      TCTGTGGCTAACAAACTGGGCAGCTTTGGCAAAACCTTGGGCAGCAAGCTCAAGAAGAAC
                                                                                

HsCD00585611        ------------------------------------------------------------
NM_020205.3         ATGGGGGGCCTGATGCACAGCAAGGGTTCAAAGCCTGGAGGGGTGGGGACAGGGTTGGGA
XM_005277414.1      ATGGGGGGCCTGATGCACAGCAAGGGTTCAAAGCCTGGAGGGGTGGGGACAGGGTTGGGA
                                                                                

HsCD00585611        ------------------------------------------------------------
NM_020205.3         GGAAGCAGCGGCACTGAGACACTGGAGAAGAAGAAGAAAAACTCACTGAAGAGCTGGAAG
XM_005277414.1      GGAAGCAGCGGCACTGAGACACTGGAGAAGAAGAAGAAAAACTCACTGAAGAGCTGGAAG
                                                                                

HsCD00585611        ------------------------------------------------------------
NM_020205.3         GGTGGCAAGGAGGAGGCAGCTGGGGATGGGCCTGTGTCTGAGAAGCCCCCAGCTGAGTCT
XM_005277414.1      GGTGGCAAGGAGGAGGCAGCTGGGGATGGGCCTGTGTCTGAGAAGCCCCCAGCTGAGTCT
                                                                                

HsCD00585611        ------------------------------------------------------------
NM_020205.3         GTTGGTAACGGAGGGAGCAAGTATAGCCAGGAGGTGATGCAGAGCCTGAGCATTCTGAGG
XM_005277414.1      GTTGGTAACGGAGGGAGCAAGTATAGCCAGGAGGTGATGCAGAGCCTGAGCATTCTGAGG
                                                                                

HsCD00585611        ------------------------------------------------------------
NM_020205.3         ACTGCCATGCAAGGGGAGGGGAAGTTTATTTTTGTTGGAACCCTGAAGATGGGTCACCGT
XM_005277414.1      ACTGCCATGCAAGGGGAGGGGAAGTTTATTTTTGTTGGAACCCTGAAGATGGGTCACCGT
                                                                                

HsCD00585611        ------------------------------------------------------------
NM_020205.3         CACCAGTATCAGGAGGAAATGATCCAGCGCTACCTTTCTGATGCTGAGGAGAGATTCCTG
XM_005277414.1      CACCAGTATCAGGAGGAAATGATCCAGCGCTACCTTTCTGATGCTGAGGAGAGATTCCTG
                                                                                

HsCD00585611        ------------------------------------------------------------
NM_020205.3         GCAGAACAGAAGCAGAAGGAGGCAGAGAGGAAGATCATGAATGGAGGAATAGGGGGTGGC
XM_005277414.1      GCAGAACAGAAGCAGAAGGAGGCAGAGAGGAAGATCATGAATGGAGGAATAGGGGGTGGC
                                                                                

HsCD00585611        ------------------------------------------------------------
NM_020205.3         CCTCCTCCAGCCAAAAAGCCAGAGCCAGATGCTAGGGAAGAGCAGCCGACCGGTCCCCCA
XM_005277414.1      CCTCCTCCAGCCAAAAAGCCAGAGCCAGATGCTAGGGAAGAGCAGCCGACCGGTCCCCCA
                                                                                

HsCD00585611        ------------------------------------------------------------
NM_020205.3         GCAGAGTCCAGGGCAATGGCATTTTCCACTGGCTACCCTGGGGACTTTACTATCCCTCGG
XM_005277414.1      GCAGAGTCCAGGGCAATGGCATTTTCCACTGGCTACCCTGGGGACTTTACTATCCCTCGG
                                                                                

HsCD00585611        ------------------------------------------------------------
NM_020205.3         CCGTCTGGGGGCGGAGTCCACTGCCAGGAACCCCGGAGGCAGTTGGCAGGGGGTCCATGT
XM_005277414.1      CCGTCTGGGGGCGGAGTCCACTGCCAGGAACCCCGGAGGCAGTTGGCAGGGGGTCCATGT
                                                                                

HsCD00585611        ------------------------------------------------------------
NM_020205.3         GTCGGGGGCCTACCACCATATGCCACCTTCCCCAGACAGTGCCCTCCTGGGCGACCCTAC
XM_005277414.1      GTCGGGGGCCTACCACCATATGCCACCTTCCCCAGACAGTGCCCTCCTGGGCGACCCTAC
                                                                                

HsCD00585611        ------------------------------------------------------------
NM_020205.3         CCCCACCAGGACAGCATCCCTTCTCTGGAGCCAGGCAGCCACTCTAAGGATGGACTTCAC
XM_005277414.1      CCCCACCAGGACAGCATCCCTTCTCTGGAGCCAGGCAGCCACTCTAAGGATGGACTTCAC
                                                                                

HsCD00585611        ------------------------------------------------------------
NM_020205.3         AGGGGTGCCTTGTTACCACCCCCCTACCGAGTGGCTGATTCCTATAGCAATGGCTACAGA
XM_005277414.1      AGGGGTGCCTTGTTACCACCCCCCTACCGAGTGGCTGATTCCTATAGCAATGGCTACAGA
                                                                                

HsCD00585611        ------------------------------------------------------------
NM_020205.3         GAGCCCCCTGAGCCAGATGGATGGGCTGGAGGTCTCCGGGGCCTTCCCCCAACTCAGACC
XM_005277414.1      GAGCCCCCTGAGCCAGATGGATGGGCTGGAGGTCTCCGGGGCCTTCCCCCAACTCAGACC
                                                                                

HsCD00585611        ------------------------------------------------------------
NM_020205.3         AAATGCAAACAACCGAACTGCAGCTTCTATGGACACCCTGAGACAAACAACTTCTGTTCC
XM_005277414.1      AAATGCAAACAACCGAACTGCAGCTTCTATGGACACCCTGAGACAAACAACTTCTGTTCC
                                                                                

HsCD00585611        ------------------------------------------------------------
NM_020205.3         TGTTGTTACAGGGAAGAACTGAGGAGGAGGGAGCGGGAACCGGATGGGGAGCTCCTGGTG
XM_005277414.1      TGTTGTTACAGGGAAGAACTGAGGAGGAGGGAGCGGGAACCGGATGGGGAGCTCCTGGTG
                                                                                

HsCD00585611        ------------
NM_020205.3         CACAGGTTCTGA
XM_005277414.1      CACAGGTTCTGA

Pathways

WikiPathway :

TNF alpha Signaling Pathway

Protein

Structural Annotation Wiki :

HR3638C

UniProt :

Q6GQQ9

HPRD :

10313

Clone

Cloning Information :

625235

Original Clone ID :

HR3638C-125-443-14.12

Experimental

TargetTrack - Experimental data :

HR3638C

Organism

Species Specific ID:

56957

Genome

Chromosome :

Map Location :

1q21.2

Reagents

Labome :

OTUD7B-antibody

OTUD7B (H. sapiens )In pET15_NESG (His-tagged bacterial expression vector)

Coding Sequence Details

Recommended Growth Condition:

Vector Features:

Gene

Reference Sequence Alignment

Pathways

Protein

Clone

Experimental

Organism

Genome

Reagents

OTUD7B (H. sapiens )
In pET15_NESG (His-tagged bacterial expression vector)