DNASU Plasmid Repository • 480.965.5697 | Email

FMOD (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

TOP

Explanation of Terms


Gene: Gene Symbol:  FMOD
Gene Name:  fibromodulin
Sequence              Map: pDNR-Dual_with_human_insert.pdf
Original Clone ID: FLH119364.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDNR-Dual               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: PMID: 16512675
Title: Functional proteomics approach to investigate the biological activities of cDNAs implicated in breast cancer.
Authors: HIP
HsCD00005876

Price:  Login for Pricing
AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1131nts         Open reading frame : 1 to 1131
Reference Sequence Alignment

ATGCAGTGGACCTCCCTCCTGCTGCTGGCAGGGCTCTTCTCCCTCTCCCAGGCCCAGTAT
GAAGATGACCCTCATTGGTGGTTCCACTACCTCCGCAGCCAGCAGTCCACCTACTACGAT
CCCTATGACCCTTACCCGTATGAGACCTACGAGCCTTACCCCTATGGGGTGGATGAAGGG
CCAGCCTACACCTACGGCTCTCCATCCCCTCCAGATCCCCGCGACTGCCCCCAGGAGTGC
GACTGCCCACCCAACTTCCCCACGGCCATGTACTGTGACAATCGCAACCTCAAGTACCTG
CCCTTCGTTCCCTCCCGCATGAAGTATGTGTACTTCCAGAACAACCAGATCACCTCCATC
CAGGAAGGCGTCTTTGACAATGCCACAGGGCTGCTCTGGATTGCTCTCCACGGCAACCAG
ATCACCAGTGATAAGGTGGGCAGGAAGGTCTTCTCCAAGCTGAGGCACCTGGAGAGGCTG
TACCTGGACCACAACAACCTGACCCGGATGCCCGGTCCCCTGCCTCGATCCCTGAGAGAG
CTCCATCTCGACCACAACCAGATCTCACGGGTCCCCAACAATGCTCTGGAGGGGCTGGAG
AACCTCACGGCCTTGTACCTCCAACACAATGAGATCCAGGAAGTGGGCAGTTCCATGAGG
GGCCTCCGGTCACTGATCTTGCTGGACCTGAGTTATAACCACCTTCGGAAGGTGCCTGAT
GGGCTGCCCTCAGCTCTTGAGCAGCTGTACATGGAGCACAACAATGTCTACACCGTCCCC
GATAGCTACTTCCGGGGGGCGCCCAAGCTGCTGTATGTGCGGCTGTCCCACAACAGTCTA
ACCAACAATGGCCTGGCCTCCAACACCTTCAATTCCAGCAGCCTCCTTGAGCTAGACCTC
TCCTACAACCAGCTGCAGAAGATCCCCCCAGTCAACACCAACCTGGAGAACCTCTACCTC
CAAGGCAATAGGATCAATGAGTTCTCCATCAGCAGCTTCTGCACCGTGGTGGACGTCGTG
AACTTCTCCAAGCTGCAGGTGCTGCGCCTGGACGGGAACGAGATCAAGCGCAGCGCCATG
CCTGCCGACGCGCCCCTCTGCCTGCGCCTTGCCAGCCTCATCGAGATCTTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1212
Start on reference sequence 82
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pDNR-Dual

pDNR-Dual in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  Unknown
Description: Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from).
Comments: The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp): 4938
Parent Vector: None
Empty Vector: None
Properties: Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name: BD Biosciences Clontech
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS restriction enzyme-type multiple cloning site 45 129
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4111 4574
negative selection marker SacB SacB negative selection marker gene (sucrose sensitivity) 1057 2972
primer site M13 primer M13 primer sequencing site 4839 4855
primer site T7 primer T7 RNA Pol primer sequencing site 4865 4883
recombination site loxP site loxP recombination site 1015 1048
recombination site loxP site loxP recombination site 9 42
selectable marker CmR chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) 986 327
selectable marker AmpR ampicillin resistance gene 3103 3963
splice donor site SD site splice donor site 132 136
tag HN 6xHN epitope tag 141 176

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : FMOD
Symbol Nomenclature : FMOD
SYNONYM : FM; SLRR2E
Designation : KSPG fibromodulin|collagen-binding 59 kDa protein|keratan sulfate proteoglycan fibromodulin
Full Nomenclature : fibromodulin
GENEID : 2331
GI : 60810939
GenBank Accession : AY893119
HGNC : 3774
MIM : 600245
Vega : OTTHUMG00000035910
Target GenBank: BC035281

 

Reference Sequence Alignment

  

HsCD00005876      ATGCAGTGGACCTCCCTCCTGCTGCTGGCAGGGCTCTTCTCCCTCTCCCAGGCCCAGTAT
NM_002023.4       ATGCAGTGGACCTCCCTCCTGCTGCTGGCAGGGCTCTTCTCCCTCTCCCAGGCCCAGTAT
                  ************************************************************

HsCD00005876      GAAGATGACCCTCATTGGTGGTTCCACTACCTCCGCAGCCAGCAGTCCACCTACTACGAT
NM_002023.4       GAAGATGACCCTCATTGGTGGTTCCACTACCTCCGCAGCCAGCAGTCCACCTACTACGAT
                  ************************************************************

HsCD00005876      CCCTATGACCCTTACCCGTATGAGACCTACGAGCCTTACCCCTATGGGGTGGATGAAGGG
NM_002023.4       CCCTATGACCCTTACCCGTATGAGACCTACGAGCCTTACCCCTATGGGGTGGATGAAGGG
                  ************************************************************

HsCD00005876      CCAGCCTACACCTACGGCTCTCCATCCCCTCCAGATCCCCGCGACTGCCCCCAGGAGTGC
NM_002023.4       CCAGCCTACACCTACGGCTCTCCATCCCCTCCAGATCCCCGCGACTGCCCCCAGGAGTGC
                  ************************************************************

HsCD00005876      GACTGCCCACCCAACTTCCCCACGGCCATGTACTGTGACAATCGCAACCTCAAGTACCTG
NM_002023.4       GACTGCCCACCCAACTTCCCCACGGCCATGTACTGTGACAATCGCAACCTCAAGTACCTG
                  ************************************************************

HsCD00005876      CCCTTCGTTCCCTCCCGCATGAAGTATGTGTACTTCCAGAACAACCAGATCACCTCCATC
NM_002023.4       CCCTTCGTTCCCTCCCGCATGAAGTATGTGTACTTCCAGAACAACCAGATCACCTCCATC
                  ************************************************************

HsCD00005876      CAGGAAGGCGTCTTTGACAATGCCACAGGGCTGCTCTGGATTGCTCTCCACGGCAACCAG
NM_002023.4       CAGGAAGGCGTCTTTGACAATGCCACAGGGCTGCTCTGGATTGCTCTCCACGGCAACCAG
                  ************************************************************

HsCD00005876      ATCACCAGTGATAAGGTGGGCAGGAAGGTCTTCTCCAAGCTGAGGCACCTGGAGAGGCTG
NM_002023.4       ATCACCAGTGATAAGGTGGGCAGGAAGGTCTTCTCCAAGCTGAGGCACCTGGAGAGGCTG
                  ************************************************************

HsCD00005876      TACCTGGACCACAACAACCTGACCCGGATGCCCGGTCCCCTGCCTCGATCCCTGAGAGAG
NM_002023.4       TACCTGGACCACAACAACCTGACCCGGATGCCCGGTCCCCTGCCTCGATCCCTGAGAGAG
                  ************************************************************

HsCD00005876      CTCCATCTCGACCACAACCAGATCTCACGGGTCCCCAACAATGCTCTGGAGGGGCTGGAG
NM_002023.4       CTCCATCTCGACCACAACCAGATCTCACGGGTCCCCAACAATGCTCTGGAGGGGCTGGAG
                  ************************************************************

HsCD00005876      AACCTCACGGCCTTGTACCTCCAACACAATGAGATCCAGGAAGTGGGCAGTTCCATGAGG
NM_002023.4       AACCTCACGGCCTTGTACCTCCAACACAATGAGATCCAGGAAGTGGGCAGTTCCATGAGG
                  ************************************************************

HsCD00005876      GGCCTCCGGTCACTGATCTTGCTGGACCTGAGTTATAACCACCTTCGGAAGGTGCCTGAT
NM_002023.4       GGCCTCCGGTCACTGATCTTGCTGGACCTGAGTTATAACCACCTTCGGAAGGTGCCTGAT
                  ************************************************************

HsCD00005876      GGGCTGCCCTCAGCTCTTGAGCAGCTGTACATGGAGCACAACAATGTCTACACCGTCCCC
NM_002023.4       GGGCTGCCCTCAGCTCTTGAGCAGCTGTACATGGAGCACAACAATGTCTACACCGTCCCC
                  ************************************************************

HsCD00005876      GATAGCTACTTCCGGGGGGCGCCCAAGCTGCTGTATGTGCGGCTGTCCCACAACAGTCTA
NM_002023.4       GATAGCTACTTCCGGGGGGCGCCCAAGCTGCTGTATGTGCGGCTGTCCCACAACAGTCTA
                  ************************************************************

HsCD00005876      ACCAACAATGGCCTGGCCTCCAACACCTTCAATTCCAGCAGCCTCCTTGAGCTAGACCTC
NM_002023.4       ACCAACAATGGCCTGGCCTCCAACACCTTCAATTCCAGCAGCCTCCTTGAGCTAGACCTC
                  ************************************************************

HsCD00005876      TCCTACAACCAGCTGCAGAAGATCCCCCCAGTCAACACCAACCTGGAGAACCTCTACCTC
NM_002023.4       TCCTACAACCAGCTGCAGAAGATCCCCCCAGTCAACACCAACCTGGAGAACCTCTACCTC
                  ************************************************************

HsCD00005876      CAAGGCAATAGGATCAATGAGTTCTCCATCAGCAGCTTCTGCACCGTGGTGGACGTCGTG
NM_002023.4       CAAGGCAATAGGATCAATGAGTTCTCCATCAGCAGCTTCTGCACCGTGGTGGACGTCGTG
                  ************************************************************

HsCD00005876      AACTTCTCCAAGCTGCAGGTGCTGCGCCTGGACGGGAACGAGATCAAGCGCAGCGCCATG
NM_002023.4       AACTTCTCCAAGCTGCAGGTGCTGCGCCTGGACGGGAACGAGATCAAGCGCAGCGCCATG
                  ************************************************************

HsCD00005876      CCTGCCGACGCGCCCCTCTGCCTGCGCCTTGCCAGCCTCATCGAGATCTTG
NM_002023.4       CCTGCCGACGCGCCCCTCTGCCTGCGCCTTGCCAGCCTCATCGAGATCTGA
                  ************************************************* .

Pathways

Reactome : Disease
Reactome : ECM proteoglycans
Reactome : Extracellular matrix organization
Reactome : Glycogen storage diseases
Reactome : Glycosaminoglycan metabolism
Reactome : Keratan sulfate biosynthesis
Reactome : Keratan sulfate degradation
Reactome : Keratan sulfate/keratin metabolism
Reactome : MPS I - Hurler syndrome
Reactome : MPS II - Hunter syndrome
Reactome : MPS IIIA - Sanfilippo syndrome A
Reactome : MPS IIIB - Sanfilippo syndrome B
Reactome : MPS IIIC - Sanfilippo syndrome C
Reactome : MPS IIID - Sanfilippo syndrome D
Reactome : MPS IV - Morquio syndrome A
Reactome : MPS IV - Morquio syndrome B
Reactome : MPS IX - Natowicz syndrome
Reactome : MPS VI - Maroteaux-Lamy syndrome
Reactome : MPS VII - Sly syndrome
Reactome : Metabolism
Reactome : Metabolism of carbohydrates
Reactome : Mucopolysaccharidoses
Reactome : Myoclonic epilepsy of Lafora

Clone

Cloning Information : 119364
HIP Master Clone ID : 95018
Original Clone ID : FLH119364.01L

Organism


TAX_ID : 9606
Species Specific ID: 2331

Genome

Chromosome : 1
Map Location : 1q32
Ensembl : ENSG00000122176

Reagents

Labome : fibromodulin-antibody