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Detailed Plasmid Information

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Explanation of Terms


Gene: Gene Symbol:  None
Gene Name:  None
Sequence             
Original Clone ID: APC109399.102
Keyword: None
Species: Streptomyces albus
Type: cDNA
Vector Name: pMCSG68               Format:  CLOSED
Source: Midwest Center for Structural Genomics
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: Midwest Center for Structural Genomics
Argonne National Laboratory
PSI:Biology
SaCD00590635

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AVAILABLE
No restriction
Special MTA: PSI

 

Insert sequence: 492nts         Open reading frame : 51 to 542

ATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCCACCCGCTCC
TCACGTCCCGCGTCGAACTCAGCGACGGCGGCACCGTGTTCAGCGGCGAGGTGTCGCTCG
GCACGCACCCCTGGATCGCGGACCACGCGGTGTCCGGCACGGTCCTCTTCCCCGGCACCG
GCTTCGTCGAGATGGCGCTGCACGCCGGGCGGGCCCTCGGCTGCGGGCGCCTCGACGAAC
TCACCCTCGAAGCTCCGCTCACCCTGACGGAACAGGCGCCGGAAGGCGCACAACTCCAGC
TCGTCGTGGGAACGCCCGACACCACGGGCAGCCGCCCCGTGGAGGTGCACTCACGCCCAC
TCGCCGCCGAGGGAGCATGGACGCGGCACGCCGCCGGGCTGCTCGCGGCGGACGGGACGG
AGGCCGGTTTCGATCTGACGGCGTGGCCGCCGGCCGGCGCGCAGTCCGTACCGGTGGACG
ACTACTACCAGGTGATGGCCGACGCCGGGTACGCCTACGGCCCCGCGTTCCGCGGGCTGT
AACATTGGAAGTGGATAATAA


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: ProteinExpressed: Protein_Confirmed
ProteinPurified: Not_Tested
SolubleProtein: Protein_Soluble
Find experimental protocols in MCSG-APC109399
Recommended expression in: E.coli BL21 (DE3)
       DyNA Vector Map

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Vector Name: pMCSG68

pMCSG68 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  T7short
Reverse:  T7 terminator
Description: Bacterial expression vector with a T7 promoter and TEV-cleavable N-terminal 6xHis and StrepII tags; rare argU and ileX codons; ampicillin resistance in bacteria; ligation independent cloning
Comments: BglI-XbaI fragment removed from pMCSG57 and ligated into pMCSG53 digested with the same enzymes
Size (bp): 4832
Parent Vector: None
Empty Vector: EvNO00450864
Properties: bacterial expression, ligation independent cloning (LIC), with tag/fusion/marker
Author Name: Midwest Center for Structural Genomics
Midwest Center for Structural Genomics
PSI:Biology
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pBR322 origin pBR322 origin of replication 2825 3413
ligation independent cloning LIC ligation independent cloning site 204 235
promoter T7 T7 promoter 384 400
protease cleavage site TEV TEV protease cleavage site 223 243
repressor protein gene LacI Lac I gene 1437 2396
selectable marker AmpR ampicillin resistance gene 3584 4444
tRNA ileX ileX tRNA gene 983 1134
tRNA argU argU tRNA gene 467 853
tag His N-terminal 6xHis tag 292 309
tag strep N-terminal strepII tag 250 273
trxn regulatory element lac operator lac operator sequence 359 381
trxn termination sequence T7 term T7 terminator 26 72

 

  • Gene
  • Protein
  • Clone
  • Experimental

Gene

Target GenBank: None

 

Protein

SMART domain : PKS_DH :
Structural Annotation Wiki : MCSG-APC109399

Clone

Cloning Information : 651079
Original Clone ID : APC109399.102

Experimental

TargetTrack - Experimental data : MCSG-APC109399