Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
Full Length: No; Antibiotic: Gentamicin; NT position: 91-2241; AA position: 31-747
Species:
Human herpesvirus 1
Type:
cDNA
Vector Name:
pDONR207
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
Xiaofang Bian
Xiaofang Bian
HhCD00594328
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 2157nts
Open reading frame: 1 to 2157
ATGGCTCCGAGTTCCCCCGGCACGCCTGGGGTCGCGGCCGCGACCCAGGCGGCGAACGGG
GGCCCTGCCACTCCGGCGCCGCCCGCCCCTGGCGCCCCCCCAACGGGGGACCCGAAACCG
AAGAAGAACAGAAAACCGAAACCCCCAAAGCCGCCGCGCCCCGCCGGCGACAACGCGACC
GTCGCCGCGGGCCACGCCACCCTGCGCGAGCACCTGCGGGACATCAAGGCGGAGAACACC
GATGCAAACTTTTACGTGTGCCCACCCCCCACGGGCGCCACGGTGGTGCAGTTCGAGCAG
CCGCGCCGCTGCCCGACCCGGCCCGAGGGTCAGAACTACACGGAGGGCATCGCGGTGGTC
TTCAAGGAGAACATCGCCCCGTACAAGTTCAAGGCCACCATGTACTACAAAGACGTCACC
GTTTCGCAGGTGTGGTTCGGCCACCGCTACTCCCAGTTTATGGGGATCTTTGAGGACCGC
GCCCCCGTCCCCTTCGAGGAGGTGATCGACAAGATCAACGCCAAGGGGGTCTGTCGGTCC
ACGGCCAAGTACGTGCGCAACAACCTGGAGACCACCGCGTTTCACCGGGACGACCACGAG
ACCGACATGGAGCTGAAACCGGCCAACGCCGCGACCCGCACGAGCCGGGGCTGGCACACC
ACCGACCTCAAGTACAACCCCTCGCGGGTGGAGGCGTTCCACCGGTACGGGACGACGGTA
AACTGCATCGTCGAGGAGGTGGACGCGCGCTCGGTGTACCCGTACGACGAGTTTGTGTTG
GCGACTGGCGACTTTGTGTACATGTCCCCGTTTTACGGCTACCGGGAGGGGTCGCACACC
GAACACACCAGCTACGCCGCCGACCGCTTCAAGCAGGTCGACGGCTTCTACGCGCGCGAC
CTCACCACCAAGGCCCGGGCCACGGCGCCGACCACCCGGAACCTGCTCACGACCCCCAAG
TTCACCGTGGCCTGGGACTGGGTGCCAAAGCGCCCGTCGGTCTGCACCATGACCAAGTGG
CAGGAGGTGGACGAGATGCTGCGCTCCGAGTACGGCGGCTCCTTCCGATTCTCTTCCGAC
GCCATATCCACCACCTTCACCACCAACCTGACCGAGTACCCGCTCTCGCGCGTGGACCTG
GGGGACTGCATCGGCAAGGACGCCCGCGACGCCATGGACCGCATCTTCGCCCGCAGGTAC
AACGCGACGCACATCAAGGTGGGCCAGCCGCAGTACTACCTGGCCAATGGGGGCTTTCTG
ATCGCGTACCAGCCCCTTCTCAGCAACACGCTCGCGGAGCTGTACGTGCGGGAACACCTC
CGCGAGCAGAGCCGCAAGCCCCCAAACCCCACGCCCCCGCCGCCCGGGGCCAGCGCCAAC
GCGTCCGTGGAGCGCATCAAGACCACCTCCTCCATCGAGTTCGCCAGGCTGCAGTTTACG
TACAACCACATACAGCGCCATGTCAACGATATGTTGGGCCGCGTTGCCATCGCGTGGTGC
GAGCTGCAGAATCACGAGCTGACCCTGTGGAACGAGGCCCGCAAGCTGAACCCCAACGCC
ATCGCCTCGGCCACCGTGGGCCGGCGGGTGAGCGCGCGGATGCTCGGCGACGTGATGGCC
GTCTCCACGTGCGTGCCGGTCGCCGCGGACAACGTGATCGTCCAAAACTCGATGCGCATC
AGCTCGCGGCCCGGGGCCTGCTACAGCCGCCCCCTGGTCAGCTTTCGGTACGAAGACCAG
GGCCCGTTGGTCGAGGGGCAGCTGGGGGAGAACAACGAGCTGCGGCTGACGCGCGATGCG
ATCGAGCCGTGCACCGTGGGACACCGGCGCTACTTCACCTTCGGTGGGGGCTACGTGTAC
TTCGAGGAGTACGCGTACTCCCACCAGCTGAGCCGCGCCGACATCACCACCGTCAGCACC
TTCATCGACCTCAACATCACCATGCTGGAGGATCACGAGTTTGTCCCCCTGGAGGTGTAC
ACCCGCCACGAGATCAAGGACAGCGGCCTGCTGGACTACACGGAGGTCCAGCGCCGCAAC
CAGCTGCACGACCTGCGCTTCGCCGACATCGACACGGTCATCCACGCCGACGCCAACGCC
GCCATGTTTGCGGGCCTGGGCGCGTTCTTCGAGGGGATGGGCGACCTGGGGCGCTAG
Protein sequence predicted based on nucleotide sequence
MAPSSPGTPGVAAATQAANGGPATPAPPAPGAPPTGDPKPKKNRKPKPPKPPRPAGDNAT VAAGHATLREHLRDIKAENTDANFYVCPPPTGATVVQFEQPRRCPTRPEGQNYTEGIAVV FKENIAPYKFKATMYYKDVTVSQVWFGHRYSQFMGIFEDRAPVPFEEVIDKINAKGVCRS TAKYVRNNLETTAFHRDDHETDMELKPANAATRTSRGWHTTDLKYNPSRVEAFHRYGTTV NCIVEEVDARSVYPYDEFVLATGDFVYMSPFYGYREGSHTEHTSYAADRFKQVDGFYARD LTTKARATAPTTRNLLTTPKFTVAWDWVPKRPSVCTMTKWQEVDEMLRSEYGGSFRFSSD AISTTFTTNLTEYPLSRVDLGDCIGKDARDAMDRIFARRYNATHIKVGQPQYYLANGGFL IAYQPLLSNTLAELYVREHLREQSRKPPNPTPPPPGASANASVERIKTTSSIEFARLQFT YNHIQRHVNDMLGRVAIAWCELQNHELTLWNEARKLNPNAIASATVGRRVSARMLGDVMA VSTCVPVAADNVIVQNSMRISSRPGACYSRPLVSFRYEDQGPLVEGQLGENNELRLTRDA IEPCTVGHRRYFTFGGGYVYFEEYAYSHQLSRADITTVSTFIDLNITMLEDHEFVPLEVY TRHEIKDSGLLDYTEVQRRNQLHDLRFADIDTVIHADANAAMFAGLGAFFEGMGDLGR*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
gentamicin
Bacterial Selection Condition:
gentamycin
Growth Condition:
None
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR207
Synonyms:
''
Sequencing Primer:
Forward:
pDONR201-reverse
Reverse:
pDONR201-forward
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and attP1 and attP2 and ccdB and CmR and cat_promoter-var002 and GenR and Promoter (Amp-R) and ori; gentamicin resistance in bacterial;
Comments:
None
Size (bp):
5585
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
GemR
confers resistance to gentamycin
3528
4061
CDS
CmR
confers resistance to chloramphenicol
1606
2265
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
959
1264
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
73
100
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
192
278
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2266
2368
promoter
Pc promoter
class 1 integron promoter
4250
4278
protein binding site
attP2
recombination site for the Gateway BP reaction
2513
2744
protein binding site
attP1
recombination site for the Gateway BP reaction
332
563
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4935
5523
