DNASU Plasmid | NR6A1 (H. sapiens )<br>In pET15_NESG (His-tagged bacterial expression vector)

Explanation of Terms

Gene:

Gene Symbol: NR6A1

Gene Name: nuclear receptor subfamily 6, group A, member 1

Original Clone ID:

HR7049C-49-143-15.2

Keyword:

targeted domain

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pET15_NESG

Format:

FUSION

Source:

Northeast Structural Genomics Consortium

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Rutgers University
PSI:Biology
Northeast Structural Genomics Consortium

HsCD00596850

Price:

AVAILABLE

No restriction
Special MTA: PSI/NESG

Insert Sequence
Protein Sequence

Insert sequence: 288nts

Open reading frame: 51 to 338

Reference Sequence Alignment

CTTTAAGAAGGAGATATACCATGGGCCATCACCATCACCATCACAGCCATATGGATCGGG
CTGAACAACGAACCTGTCTCATTTGTGGGGACCGCGCTACAGGCTTGCACTATGGGATCA
TCTCCTGTGAGGGCTGCAAAGGGTTTTTCAAGCGGAGCATTTGCAACAAACGGGTATATC
GATGCAGTCGTGACAAGAACTGTGTCATGTCTCGGAAGCAGAGGAACAGGTGCCAGTACT
GCCGCCTGCTCAAATGCCTCCAGATGGGGATGAACCGGAAGGCTATCAGAGAAGGTGGCA
TGCCTGGAGGCCGGAATAAGAGCATTGGGCCAGTCCAGATATCGGAAGAAGAAATCGAAA
GGATCATGTCTGGGCAGGAGTTTGAGTAG

Protein sequence predicted based on nucleotide sequence

MDRAEQRTCLICGDRATGLHYGIISCEGCKGFFKRSICNKRVYRCSRDKNCVMSRKQRNR
CQYCRLLKCLQMGMNRKAIREGGMPGGRNKSIGPVQ

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

a245g,D82G;ins@286,48

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

ProteinPurified: Not_Tested

ProteinExpressed: Protein_Confirmed

SolubleProtein: Protein_Soluble

Find experimental protocols in HR7049C

Recommended expression in:

Not Applicable

Vector Name:

pET15_NESG

Synonyms:

pET15, pET15-modified, pET15Cc, pET15_NESG

Sequencing Primer:

Forward: T7

Reverse: T7 terminator

Description:

Bacterial (E. coli) expression vector with T7 lac promoter, adds N-terminal 6x His tag; amp resistance; restriction enzyme cloning.

Comments:

Modified Novagen pET vector-common MCS

Size (bp):

5723

Parent Vector:

None

Properties:

bacterial expression, in vitro transcription, multiple cloning site, with tag/fusion/marker

Author Name:

Gaetano T. Montleione
Jessica Bearden
Northeast Structural Genomics Consortium

Publications:

PMID: 15808222

Title: Robotic cloning and Protein Production Platform of the Northeast Structural Genomics Consortium

Vector Map:

Vector Sequence:

Sequence:

TTCTCATGTTTGACAGCTTATCATCGATAAGCTAGCTTTAATGCGGTAGTTTATCACAGTTAAATTGCTAACGCAGTCAGGCACCGTGTATGAAATCTAACAATGCGCTCATCGTCATCCTCGGCACCGTCACCCTGGATGCTGTAGGCATAGGCTTGGTTATGCCGGTACTGCCGGGCCTCTTGCGGGATATCCGGATATAGTTCCTCCTTTCAGCAAAAAACCCCTCAAGACCCGTTTAGAGGCCCCAAGGGGTTATGCTAGTTATTGCTCAGCGGTGGCAGCAGCCAACTCAGCTTCCTTTCGGGCTTTGTTAGCAGCCGGATCTCGAGAAGCTTTGCGTCGACAGAGCTCGCAGGATCCGCAGAATTCGCCATATGGCTGTGATGGTGATGGTGATGGCCCATGGTATATCTCCTTCTTAAAGTTAAACAAAATTATTTCTAGAGGGGAATTGTTATCCGCTCACAATTCCCCTATAGTGAGTCGTATTAATTTCGCGGGATCGAGATCTCGATCCTCTACGCCGGACGCATCGTGGCCGGCATCACCGGCGCCACAGGTGCGGTTGCTGGCGCCTATATCGCCGACATCACCGATGGGGAAGATCGGGCTCGCCACTTCGGGCTCATGAGCGCTTGTTTCGGCGTGGGTATGGTGGCAGGCCCCGTGGCCGGGGGACTGTTGGGCGCCATCTCCTTGCATGCACCATTCCTTGCGGCGGCGGTGCTCAACGGCCTCAACCTACTACTGGGCTGCTTCCTAATGCAGGAGTCGCATAAGGGAGAGCGTCGAGATCCCGGACACCATCGAATGGCGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAATGTGAAACCAGTAACGTTATACGATGTCGCAGAGTATGCCGGTGTCTCTTATCAGACCGTTTCCCGCGTGGTGAACCAGGCCAGCCACGTTTCTGCGAAAACGCGGGAAAAAGTGGAAGCGGCGATGGCGGAGCTGAATTACATTCCCAACCGCGTGGCACAACAACTGGCGGGCAAACAGTCGTTGCTGATTGGCGTTGCCACCTCCAGTCTGGCCCTGCACGCGCCGTCGCAAATTGTCGCGGCGATTAAATCTCGCGCCGATCAACTGGGTGCCAGCGTGGTGGTGTCGATGGTAGAACGAAGCGGCGTCGAAGCCTGTAAAGCGGCGGTGCACAATCTTCTCGCGCAACGCGTCAGTGGGCTGATCATTAACTATCCGCTGGATGACCAGGATGCCATTGCTGTGGAAGCTGCCTGCACTAATGTTCCGGCGTTATTTCTTGATGTCTCTGACCAGACACCCATCAACAGTATTATTTTCTCCCATGAAGACGGTACGCGACTGGGCGTGGAGCATCTGGTCGCATTGGGTCACCAGCAAATCGCGCTGTTAGCGGGCCCATTAAGTTCTGTCTCGGCGCGTCTGCGTCTGGCTGGCTGGCATAAATATCTCACTCGCAATCAAATTCAGCCGATAGCGGAACGGGAAGGCGACTGGAGTGCCATGTCCGGTTTTCAACAAACCATGCAAATGCTGAATGAGGGCATCGTTCCCACTGCGATGCTGGTTGCCAACGATCAGATGGCGCTGGGCGCAATGCGCGCCATTACCGAGTCCGGGCTGCGCGTTGGTGCGGATATCTCGGTAGTGGGATACGACGATACCGAAGACAGCTCATGTTATATCCCGCCGTTAACCACCATCAAACAGGATTTTCGCCTGCTGGGGCAAACCAGCGTGGACCGCTTGCTGCAACTCTCTCAGGGCCAGGCGGTGAAGGGCAATCAGCTGTTGCCCGTCTCACTGGTGAAAAGAAAAACCACCCTGGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTAAGTTAGCTCACTCATTAGGCACCGGGATCTCGACCGATGCCCTTGAGAGCCTTCAACCCAGTCAGCTCCTTCCGGTGGGCGCGGGGCATGACTATCGTCGCCGCACTTATGACTGTCTTCTTTATCATGCAACTCGTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGCGCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCCTTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATGGCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTCCCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTGTCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACCAGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGCCTCGGCGAGCACATGGAACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGTCGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACGGATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAACCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCATCTCGGGCAGCGTTGGGTCCTGGCCACGGGTGCGCATGATCGTGCTCCTGTCGTTGAGGACCCGGCTAGGCTGGCGGGGTTGCCTTACTGGTTAGCAGAATGAATCACCGATACGCGAGCGAACGTGAAGCGACTGCTGCTGCAAAACGTCTGCGACCTGAGCAACAACATGAATGGTCTTCGGTTTCCGTGTTTCGTAAAGTCTGGAAACGCGGAAGTCAGCGCCCTGCACCATTATGTTCCGGATCTGCATCGCAGGATGCTGCTGGCTACCCTGTGGAACACCTACATCTGTATTAACGAAGCGCTGGCATTGACCCTGAGTGATTTTTCTCTGGTCCCGCCGCATCCATACCGCCAGTTGTTTACCCTCACAACGTTCCAGTAACCGGGCATGTTCATCATCAGTAACCCGTATCGTGAGCATCCTCTCTCGTTTCATCGGTATCATTACCCCCATGAACAGAAATCCCCCTTACACGGAGGCATCAGTGACCAAACAGGAAAAAACCGCCCTTAACATGGCCCGCTTTATCAGAAGCCAGACATTAACGCTTCTGGAGAAACTCAACGAGCTGGACGCGGATGAACAGGCAGACATCTGTGAATCGCTTCACGACCACGCTGATGAGCTTTACCGCAGCTGCCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTGCAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAACACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTTCAAGAA

Vector Features:

Type

Name

Description

Start

End

MCS

MCS (includes SphI, XbaI, EcoRI, BamHI, XhoI)

327

452

bacterial origin

ori

ColE1 origin of replication

3881

4563

gene fragment

ROP

repressor of primer ROP sequence

3281

3457

primer

T7 forward

T7 forward sequencing primer

481

496

primer

T7 reverse

T7 reverse sequencing primer

257

275

promoter

T7 promoter

478

497

repressor binding site

lacO

lacO regulatory sequence

456

478

selectable marker

AmpR

ampicillin resistance gene

4661

5230

tag

His

N-terminal 6x His tag

387

404

trxn termination sequence

T7 term

T7 termination sequence

257

275

Gene

Gene Symbol :

NR6A1

Symbol Nomenclature :

NR6A1

SYNONYM :

CT150; GCNF; GCNF1; NR61; RTR; hGCNF; hRTR

Designation :

germ cell nuclear factor variant 1|nuclear receptor subfamily 6 group A member 1|retinoic acid receptor-related testis-associated receptor|retinoid receptor-related testis-specific receptor

Full Nomenclature :

nuclear receptor subfamily 6, group A, member 1

GENEID :

2649

GenBank Accession :

NM_033335

HGNC :

7985

MIM :

602778

Vega :

OTTHUMG00000020661

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00596850        -----------------------------------------CTTTAAGAAGGAGATATAC
NM_001489.4         ATGGAGCGGGACGAACCGCCGCCTAGCGGAGGGGGAGGCGGCGGGGGCTCGGCGGGGTTC
NM_001278546.1      ATGGAGCGGGACGAACCGCCGCCTAGCGGAGGGGGAGGCGGCGGGGGCTCGGCGGGGTTC
NM_033334.3         ATGGAGCGGGACGAACCGCCGCCTAGCGGAGGGGGAGGCGGCGGGGGCTCGGCGGGGTTC
                                                             *   .. :.**.*. .*:*

HsCD00596850        CATGGGCCATCACCATCACCATCACAGCCATATG--------------------------
NM_001489.4         CTGGAGCCTCCCGCCGCGCTCCCTCCGCCGCCGCGCAACGGTTTCTGTCAGGATGAATTG
NM_001278546.1      CTGGAGCCTCCCGCCGCGCTCCCTCCGCCGCCGCGCAACGGTTTCTGTCAGGATGAATTG
NM_033334.3         CTGGAGCCTCCCGCCGCGCTCCCTCCGCCGCCGCGCAACGGTTTCTGTCAGGATGAATTG
                    *: *.***: *. *. *.* . *:*.***. .                            

HsCD00596850        ------------------------------------GATCGGGCTGAACAACGAACCTGT
NM_001489.4         GCAGAGCTTGACCCAG------------GCACTAATGATCGGGCTGAACAACGAACCTGT
NM_001278546.1      GCAGAGCTTGACCCAG------------GCACTAATGATCGGGCTGAACAACGAACCTGT
NM_033334.3         GCAGAGCTTGACCCAGGCACTATTTCTGTTTCAGATGATCGGGCTGAACAACGAACCTGT
                                                        ************************

HsCD00596850        CTCATTTGTGGGGACCGCGCTACAGGCTTGCACTATGGGATCATCTCCTGTGAGGGCTGC
NM_001489.4         CTCATTTGTGGGGACCGCGCTACAGGCTTGCACTATGGGATCATCTCCTGTGAGGGCTGC
NM_001278546.1      CTCATTTGTGGGGACCGCGCTACAGGCTTGCACTATGGGATCATCTCCTGTGAGGGCTGC
NM_033334.3         CTCATTTGTGGGGACCGCGCTACAGGCTTGCACTATGGGATCATCTCCTGTGAGGGCTGC
                    ************************************************************

HsCD00596850        AAAGGGTTTTTCAAGCGGAGCATTTGCAACAAACGGGTATATCGATGCAGTCGTGACAAG
NM_001489.4         AAAGGGTTTTTCAAGCGGAGCATTTGCAACAAACGGGTATATCGATGCAGTCGTGACAAG
NM_001278546.1      AAAGGGTTTTTCAAGCGGAGCATTTGCAACAAACGGGTATATCGATGCAGTCGTGACAAG
NM_033334.3         AAAGGGTTTTTCAAGCGGAGCATTTGCAACAAACGGGTATATCGATGCAGTCGTGACAAG
                    ************************************************************

HsCD00596850        AACTGTGTCATGTCTCGGAAGCAGAGGAACAGGTGCCAGTACTGCCGCCTGCTCAAATGC
NM_001489.4         AACTGTGTCATGTCTCGGAAGCAGAGGAACAGGTGCCAGTACTGCCGCCTGCTCAAATGC
NM_001278546.1      AACTGTGTCATGTCTCGGAAGCAGAGGAACAGGTGCCAGTACTGCCGCCTGCTCAAATGC
NM_033334.3         AACTGTGTCATGTCTCGGAAGCAGAGGAACAGGTGCCAGTACTGCCGCCTGCTCAAATGC
                    ************************************************************

HsCD00596850        CTCCAGATGGGGATGAACCGGAAGGCTATCAGAGAAGGTGGCATGCCTGGAGGCCGGAAT
NM_001489.4         CTCCAGATGGGGATGAACCGGAAGGCTATCAGAGAAGATGGCATGCCTGGAGGCCGGAAT
NM_001278546.1      CTCCAGATGGGGATGAACCGGAAGGCTATCAGAGAAGATGGCATGCCTGGAGGCCGGAAT
NM_033334.3         CTCCAGATGGGGATGAACCGGAAGGCTATCAGAGAAGATGGCATGCCTGGAGGCCGGAAT
                    *************************************.**********************

HsCD00596850        AAGAGCATTGGGCCAGTCCAGATATCGGAAGAAGAAATCGAAAGGATCATGTCTGGGCAG
NM_001489.4         AAGAGCATTGGGCCAGTCCAGATATCGGAAGAAGAAATCGAAAGGATCATGTCTGGGCAG
NM_001278546.1      AAGAGCATTGGGCCAGTCCAGATATCGGAAGAAGAAATCGAAAGGATCATGTCTGGGCAG
NM_033334.3         AAGAGCATTGGGCCAGTCCAGATATCGGAAGAAGAAATCGAAAGGATCATGTCTGGGCAG
                    ************************************************************

HsCD00596850        GAGTTTGAGTAG------------------------------------------------
NM_001489.4         GAGTTTGAGGAAGAGGCCAATCACTGGAGCAACCATGGTGATAGTGACCACAGTTCCCCT
NM_001278546.1      GAGTTTGAGGAAGAGGCCAATCACTGGAGCAACCATGGTGATAGTGACCACAGTTCCCCT
NM_033334.3         GAGTTTGAGGAAGAGGCCAATCACTGGAGCAACCATGGTGATAGTGACCACAGTTCCCCT
                    ********* *.                                                

HsCD00596850        ------------------------------------------------------------
NM_001489.4         GGGAACAGGGCTTCGGAGAGCAACCAGCCCTCACCAGGCTCCACACTGTCTTCCAG---G
NM_001278546.1      GGGAACAGGGCTTCGGAGAGCAACCAGCCCTCACCAGGCTCCACACTGTCTTCCAGTAGG
NM_033334.3         GGGAACAGGGCTTCGGAGAGCAACCAGCCCTCACCAGGCTCCACACTGTCTTCCAGTAGG
                                                                                

HsCD00596850        ------------------------------------------------------------
NM_001489.4         TCTGTGGAACTGAATGGATTCATGGCCTTCAGGGAACAGTACATGGGAATGTCTGTGCCT
NM_001278546.1      TCTGTGGAACTGAATGGATTCATGGCCTTCAGGGAACAGTACATGGGAATGTCTGTGCCT
NM_033334.3         TCTGTGGAACTGAATGGATTCATGGCCTTCAGGGAACAGTACATGGGAATGTCTGTGCCT
                                                                                

HsCD00596850        ------------------------------------------------------------
NM_001489.4         CCACATTACCAATATATACCGCACCTTTTTAGCTATTCTGGCCACTCACCACTTCTGCCC
NM_001278546.1      CCACATTACCAATATATACCGCACCTTTTTAGCTATTCTGGCCACTCACCACTTCTGCCC
NM_033334.3         CCACATTACCAATATATACCGCACCTTTTTAGCTATTCTGGCCACTCACCACTTCTGCCC
                                                                                

HsCD00596850        ------------------------------------------------------------
NM_001489.4         CAACAAGCTCGCAGCCTGGATCCCCAGTCATACAGTCTGATTCACCAGCTGTTATCAGCC
NM_001278546.1      CAACAAGCTCGCAGCCTGGATCCCCAGTCATACAGTCTGATTCACCAGCTGTTATCAGCC
NM_033334.3         CAACAAGCTCGCAGCCTGGATCCCCAGTCATACAGTCTGATTCACCAGCTGTTATCAGCC
                                                                                

HsCD00596850        ------------------------------------------------------------
NM_001489.4         GAGGACCTGGAACCATTGGGCACGCCCATGTTGATTGAAGATGGATACGCTGTGACACAG
NM_001278546.1      GAGGACCTGGAACCATTGGGCACGCCCATGTTGATTGAAGATGGATACGCTGTGACACAG
NM_033334.3         GAGGACCTGGAACCATTGGGCACGCCCATGTTGATTGAAGATGGATACGCTGTGACACAG
                                                                                

HsCD00596850        ------------------------------------------------------------
NM_001489.4         GCAGAACTATTTGCCCTGCTTTGCCGCCTGGCCGACGAGCTGCTCTTTAGGCAGATTGCC
NM_001278546.1      GCAGAACTATTTGCCCTGCTTTGCCGCCTGGCCGACGAGCTGCTCTTTAGGCAGATTGCC
NM_033334.3         GCAGAACTATTTGCCCTGCTTTGCCGCCTGGCCGACGAGCTGCTCTTTAGGCAGATTGCC
                                                                                

HsCD00596850        ------------------------------------------------------------
NM_001489.4         TGGATCAAGAAACTGCCTTTCTTCTGCGAGCTCTCAATCAAGGATTACACGTGCCTCTTG
NM_001278546.1      TGGATCAAGAAACTGCCTTTCTTCTGCGAGCTCTCAATCAAGGATTACACGTGCCTCTTG
NM_033334.3         TGGATCAAGAAACTGCCTTTCTTCTGCGAGCTCTCAATCAAGGATTACACGTGCCTCTTG
                                                                                

HsCD00596850        ------------------------------------------------------------
NM_001489.4         AGCTCTACGTGGCAGGAGCTAATCCTGCTGTCTTCCCTCACCGTTTACAGCAAGCAGATC
NM_001278546.1      AGCTCTACGTGGCAGGAGCTAATCCTGCTGTCTTCCCTCACCGTTTACAGCAAGCAGATC
NM_033334.3         AGCTCTACGTGGCAGGAGCTAATCCTGCTGTCTTCCCTCACCGTTTACAGCAAGCAGATC
                                                                                

HsCD00596850        ------------------------------------------------------------
NM_001489.4         TTTGGGGAACTGGCTGATGTCACTGCCAAGTACTCGCCCTCCGATGAAGAACTACACAGA
NM_001278546.1      TTTGGGGAACTGGCTGATGTCACTGCCAAGTACTCGCCCTCCGATGAAGAACTACACAGA
NM_033334.3         TTTGGGGAACTGGCTGATGTCACTGCCAAGTACTCGCCCTCCGATGAAGAACTACACAGA
                                                                                

HsCD00596850        ------------------------------------------------------------
NM_001489.4         TTTAGTGATGAAGGGATGGAGGTGATCGAGCGGCTCATCTACCTCTATCACAAGTTCCAT
NM_001278546.1      TTTAGTGATGAAGGGATGGAGGTGATCGAGCGGCTCATCTACCTCTATCACAAGTTCCAT
NM_033334.3         TTTAGTGATGAAGGGATGGAGGTGATCGAGCGGCTCATCTACCTCTATCACAAGTTCCAT
                                                                                

HsCD00596850        ------------------------------------------------------------
NM_001489.4         CAGCTAAAGGTCAGCAACGAGGAGTATGCTTGCATGAAAGCAATTAACTTCCTAAATCAA
NM_001278546.1      CAGCTAAAGGTCAGCAACGAGGAGTATGCTTGCATGAAAGCAATTAACTTCCTAAATCAA
NM_033334.3         CAGCTAAAGGTCAGCAACGAGGAGTATGCTTGCATGAAAGCAATTAACTTCCTAAATCAA
                                                                                

HsCD00596850        ------------------------------------------------------------
NM_001489.4         GATATCAGGGGTCTGACCAGTGCCTCACAGCTGGAACAATTGAATAAACGATACTGGTAC
NM_001278546.1      GATATCAGGGGTCTGACCAGTGCCTCACAGCTGGAACAATTGAATAAACGATACTGGTAC
NM_033334.3         GATATCAGGGGTCTGACCAGTGCCTCACAGCTGGAACAATTGAATAAACGATACTGGTAC
                                                                                

HsCD00596850        ------------------------------------------------------------
NM_001489.4         ATTTGCCAGGATTTTACTGAATATAAATACACACATCAGCCGAACCGCTTTCCTGATCTC
NM_001278546.1      ATTTGCCAGGATTTTACTGAATATAAATACACACATCAGCCGAACCGCTTTCCTGATCTC
NM_033334.3         ATTTGCCAGGATTTTACTGAATATAAATACACACATCAGCCGAACCGCTTTCCTGATCTC
                                                                                

HsCD00596850        ------------------------------------------------------------
NM_001489.4         ATGATGTGCTTACCTGAGATTCGATATATTGCAGGAAAGATGGTGAATGTGCCCCTGGAG
NM_001278546.1      ATGATGTGCTTACCTGAGATTCGATATATTGCAGGAAAGATGGTGAATGTGCCCCTGGAG
NM_033334.3         ATGATGTGCTTACCTGAGATTCGATATATTGCAGGAAAGATGGTGAATGTGCCCCTGGAG
                                                                                

HsCD00596850        ------------------------------------------------------------
NM_001489.4         CAGCTGCCCCTCCTCTTTAAGGTGGTGCTGCATTCCTGCAAGACCAGTGTGGGCAAGGAA
NM_001278546.1      CAGCTGCCCCTCCTCTTTAAGGTGGTGCTGCATTCCTGCAAGACCAGTGTGGGCAAGGAA
NM_033334.3         CAGCTGCCCCTCCTCTTTAAGGTGGTGCTGCATTCCTGCAAGACCAGTGTGGGCAAGGAA
                                                                                

HsCD00596850        ---
NM_001489.4         TGA
NM_001278546.1      TGA
NM_033334.3         TGA

Pathways

Reactome :

Developmental Biology

Reactome :

Gene Expression

Reactome :

Generic Transcription Pathway

Reactome :

Nuclear Receptor transcription pathway

Reactome :

POU5F1 (OCT4), SOX2, NANOG activate genes related to proliferation

Reactome :

Transcriptional regulation of pluripotent stem cells

Protein

Structural Annotation Wiki :

HR7049C

UniProt :

Q15406

HPRD :

04147

Clone

Cloning Information :

625661

Original Clone ID :

HR7049C-49-143-15.2

Experimental

TargetTrack - Experimental data :

HR7049C

Organism

Species Specific ID:

2649

Genome

Chromosome :

Map Location :

9q33.3

Ensembl :

ENSG00000148200

Reagents

Labome :

germ-cell-nuclear-factor-antibody

NR6A1 (H. sapiens )In pET15_NESG (His-tagged bacterial expression vector)

Coding Sequence Details

Recommended Growth Condition:

Vector Features:

Gene

Reference Sequence Alignment

Pathways

Protein

Clone

Experimental

Organism

Genome

Reagents

NR6A1 (H. sapiens )
In pET15_NESG (His-tagged bacterial expression vector)