DNASU Plasmid | LMNB1 (H. sapiens )<br>In pET15_NESG (His-tagged bacterial expression vector)

Explanation of Terms

Gene:

Gene Symbol: LMNB1

Gene Name: lamin B1

Original Clone ID:

HR5546C-22-380-15.9

Keyword:

targeted domain

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pET15_NESG

Format:

CLOSED

Source:

Northeast Structural Genomics Consortium

Description:

None

Comments:

None

Mutation / Discrepancy:

No / Yes

Publications:

None

Authors:

Rutgers University
PSI:Biology
Northeast Structural Genomics Consortium

HsCD00598069

Price:

AVAILABLE

No restriction
Special MTA: PSI/NESG

Insert Sequence
Protein Sequence

Insert sequence: 1083nts

Open reading frame: 26 to 1108

Reference Sequence Alignment

CCATCACCATCACCATCACAGCCATATGCTGAGCCCCACGCGCCTGTCGCGGCTCCAGGA
GAAGGAGGAGCTGCGCGAGCTCAATGACCGGCTGGCGGTGTACATCGACAAGGTGCGCAG
CCTGGAGACGGAGAACCGCGCGCTGCAGCTGCAGGTGACGGAGCGCGAGGAGGTGCGCGG
CCGTGAGCTCACCGGCCTCAAGGCGCTCTACGAGACCGAGCTGGCCGACGCGCGACGCGC
GCTCGACGACACGGCCCGCGAGCGCGCCAAGCTGCAGATCGAGCCGGGCAAGTGCAAGGC
GGAACACGACCAGCTGCTCCTCAACTATGCTAGGAAGGAATCTGATCTTAATGGCGCCCA
GATCAAGCTTCGAGAATATGAAGCAGCACTGAATTCGAAAGATGCAGCTCTTGCTACTGC
ACTTGGTGACAAAAAAAGTTTAGAGGGAGATTTGGAGGATCTGAAGGATCAGATTGCCCA
GTTGGAAGCCTCCTTAGCTGCAGCCAAAAAACAGTTAGCAGATGAAACTTTACTTAAAGT
AGATTTGGAGAATCGTTGTCAGAGCCTTACTGAGGACTTGGAGTTTCGCAAAAGCATGTA
TGAAGAGGAGATTAACGAGACCAGAAGGAAGCATGAAACGCGCTTGGTAGAGGTGGATTC
TGGGCGTCAAATTGAGTATGAGTACAAGCTGGCGCAAGCCCTTCATGAGATGAGAGAGCA
ACATGATGCCCAAGTGAGGCTGTATAAGGAGGAGCTGGAGCAGACTTACCATGCCAAACT
TGAGAACGCCAGACTGTCATCAGAGATGAATACTTCTACTGTCAACAGTGCCAGGGAAGA
ACTGATGGAAAGCCGCATGAGAATCGAGAGCCTTTCATCCCAGCTTTCTAATCTACAGAA
AGAGTCTAGAGCATGTTTGGAAAGGATTCAAGAATTAGAGGACTTGCTTGCTAAAGAAAA
AGACAACTCTCGTCGCATGCTGACAGACAAAGAGAGAGAGATGGCGGAAATAAGGGATCA
AATGCAGCAACAGCTGAATGACTATGAACAGCTTCTTGATGTAAAGTTAGCCCTGGACAT
GGAAATCAGTGCTTACAGGAAACTCTAG

Protein sequence predicted based on nucleotide sequence

MLSPTRLSRLQEKEELRELNDRLAVYIDKVRSLETENRALQLQVTEREEVRGRELTGLKA
LYETELADARRALDDTARERAKLQIEPGKCKAEHDQLLLNYARKESDLNGAQIKLREYEA
ALNSKDAALATALGDKKSLEGDLEDLKDQIAQLEASLAAAKKQLADETLLKVDLENRCQS
LTEDLEFRKSMYEEEINETRRKHETRLVEVDSGRQIEYEYKLAQALHEMREQHDAQVRLY
KEELEQTYHAKLENARLSSEMNTSTVNSAREELMESRMRIESLSSQLSNLQKESRACLER
IQELEDLLAKEKDNSRRMLTDKEREMAEIRDQMQQQLNDYEQLLDVKLALDMEISAYRKL
*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

a112c,S38R;t260c,L87P;

a308g,K103R;t762c,N254N;

t840c,I280I

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

ProteinPurified: Not_Tested

ProteinExpressed: Protein_Confirmed

SolubleProtein: Tested_Not_Soluble

Find experimental protocols in HR5546C

Recommended expression in:

Not Applicable

Vector Name:

pET15_NESG

Synonyms:

pET15, pET15-modified, pET15Cc, pET15_NESG

Sequencing Primer:

Forward: T7

Reverse: T7 terminator

Description:

Bacterial (E. coli) expression vector with T7 lac promoter, adds N-terminal 6x His tag; amp resistance; restriction enzyme cloning.

Comments:

Modified Novagen pET vector-common MCS

Size (bp):

5723

Parent Vector:

None

Properties:

bacterial expression, in vitro transcription, multiple cloning site, with tag/fusion/marker

Author Name:

Gaetano T. Montleione
Jessica Bearden
Northeast Structural Genomics Consortium

Publications:

PMID: 15808222

Title: Robotic cloning and Protein Production Platform of the Northeast Structural Genomics Consortium

Vector Map:

Vector Sequence:

Sequence:

TTCTCATGTTTGACAGCTTATCATCGATAAGCTAGCTTTAATGCGGTAGTTTATCACAGTTAAATTGCTAACGCAGTCAGGCACCGTGTATGAAATCTAACAATGCGCTCATCGTCATCCTCGGCACCGTCACCCTGGATGCTGTAGGCATAGGCTTGGTTATGCCGGTACTGCCGGGCCTCTTGCGGGATATCCGGATATAGTTCCTCCTTTCAGCAAAAAACCCCTCAAGACCCGTTTAGAGGCCCCAAGGGGTTATGCTAGTTATTGCTCAGCGGTGGCAGCAGCCAACTCAGCTTCCTTTCGGGCTTTGTTAGCAGCCGGATCTCGAGAAGCTTTGCGTCGACAGAGCTCGCAGGATCCGCAGAATTCGCCATATGGCTGTGATGGTGATGGTGATGGCCCATGGTATATCTCCTTCTTAAAGTTAAACAAAATTATTTCTAGAGGGGAATTGTTATCCGCTCACAATTCCCCTATAGTGAGTCGTATTAATTTCGCGGGATCGAGATCTCGATCCTCTACGCCGGACGCATCGTGGCCGGCATCACCGGCGCCACAGGTGCGGTTGCTGGCGCCTATATCGCCGACATCACCGATGGGGAAGATCGGGCTCGCCACTTCGGGCTCATGAGCGCTTGTTTCGGCGTGGGTATGGTGGCAGGCCCCGTGGCCGGGGGACTGTTGGGCGCCATCTCCTTGCATGCACCATTCCTTGCGGCGGCGGTGCTCAACGGCCTCAACCTACTACTGGGCTGCTTCCTAATGCAGGAGTCGCATAAGGGAGAGCGTCGAGATCCCGGACACCATCGAATGGCGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAATGTGAAACCAGTAACGTTATACGATGTCGCAGAGTATGCCGGTGTCTCTTATCAGACCGTTTCCCGCGTGGTGAACCAGGCCAGCCACGTTTCTGCGAAAACGCGGGAAAAAGTGGAAGCGGCGATGGCGGAGCTGAATTACATTCCCAACCGCGTGGCACAACAACTGGCGGGCAAACAGTCGTTGCTGATTGGCGTTGCCACCTCCAGTCTGGCCCTGCACGCGCCGTCGCAAATTGTCGCGGCGATTAAATCTCGCGCCGATCAACTGGGTGCCAGCGTGGTGGTGTCGATGGTAGAACGAAGCGGCGTCGAAGCCTGTAAAGCGGCGGTGCACAATCTTCTCGCGCAACGCGTCAGTGGGCTGATCATTAACTATCCGCTGGATGACCAGGATGCCATTGCTGTGGAAGCTGCCTGCACTAATGTTCCGGCGTTATTTCTTGATGTCTCTGACCAGACACCCATCAACAGTATTATTTTCTCCCATGAAGACGGTACGCGACTGGGCGTGGAGCATCTGGTCGCATTGGGTCACCAGCAAATCGCGCTGTTAGCGGGCCCATTAAGTTCTGTCTCGGCGCGTCTGCGTCTGGCTGGCTGGCATAAATATCTCACTCGCAATCAAATTCAGCCGATAGCGGAACGGGAAGGCGACTGGAGTGCCATGTCCGGTTTTCAACAAACCATGCAAATGCTGAATGAGGGCATCGTTCCCACTGCGATGCTGGTTGCCAACGATCAGATGGCGCTGGGCGCAATGCGCGCCATTACCGAGTCCGGGCTGCGCGTTGGTGCGGATATCTCGGTAGTGGGATACGACGATACCGAAGACAGCTCATGTTATATCCCGCCGTTAACCACCATCAAACAGGATTTTCGCCTGCTGGGGCAAACCAGCGTGGACCGCTTGCTGCAACTCTCTCAGGGCCAGGCGGTGAAGGGCAATCAGCTGTTGCCCGTCTCACTGGTGAAAAGAAAAACCACCCTGGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTAAGTTAGCTCACTCATTAGGCACCGGGATCTCGACCGATGCCCTTGAGAGCCTTCAACCCAGTCAGCTCCTTCCGGTGGGCGCGGGGCATGACTATCGTCGCCGCACTTATGACTGTCTTCTTTATCATGCAACTCGTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGCGCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCCTTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATGGCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTCCCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTGTCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACCAGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGCCTCGGCGAGCACATGGAACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGTCGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACGGATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAACCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCATCTCGGGCAGCGTTGGGTCCTGGCCACGGGTGCGCATGATCGTGCTCCTGTCGTTGAGGACCCGGCTAGGCTGGCGGGGTTGCCTTACTGGTTAGCAGAATGAATCACCGATACGCGAGCGAACGTGAAGCGACTGCTGCTGCAAAACGTCTGCGACCTGAGCAACAACATGAATGGTCTTCGGTTTCCGTGTTTCGTAAAGTCTGGAAACGCGGAAGTCAGCGCCCTGCACCATTATGTTCCGGATCTGCATCGCAGGATGCTGCTGGCTACCCTGTGGAACACCTACATCTGTATTAACGAAGCGCTGGCATTGACCCTGAGTGATTTTTCTCTGGTCCCGCCGCATCCATACCGCCAGTTGTTTACCCTCACAACGTTCCAGTAACCGGGCATGTTCATCATCAGTAACCCGTATCGTGAGCATCCTCTCTCGTTTCATCGGTATCATTACCCCCATGAACAGAAATCCCCCTTACACGGAGGCATCAGTGACCAAACAGGAAAAAACCGCCCTTAACATGGCCCGCTTTATCAGAAGCCAGACATTAACGCTTCTGGAGAAACTCAACGAGCTGGACGCGGATGAACAGGCAGACATCTGTGAATCGCTTCACGACCACGCTGATGAGCTTTACCGCAGCTGCCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTGCAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAACACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTTCAAGAA

Vector Features:

Type

Name

Description

Start

End

MCS

MCS (includes SphI, XbaI, EcoRI, BamHI, XhoI)

327

452

bacterial origin

ori

ColE1 origin of replication

3881

4563

gene fragment

ROP

repressor of primer ROP sequence

3281

3457

primer

T7 forward

T7 forward sequencing primer

481

496

primer

T7 reverse

T7 reverse sequencing primer

257

275

promoter

T7 promoter

478

497

repressor binding site

lacO

lacO regulatory sequence

456

478

selectable marker

AmpR

ampicillin resistance gene

4661

5230

tag

His

N-terminal 6x His tag

387

404

trxn termination sequence

T7 term

T7 termination sequence

257

275

Gene

Gene Symbol :

LMNB1

Symbol Nomenclature :

LMNB1

SYNONYM :

ADLD; LMN; LMN2; LMNB

Designation :

lamin-B1

Full Nomenclature :

lamin B1

GENEID :

4001

GI :

5031877

GenBank Accession :

NM_005573

HGNC :

6637

MIM :

150340

Vega :

OTTHUMG00000128969

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00598069        ------------------------------------------------------------
NM_005573.3         ATGGCGACTGCGACCCCCGTGCCGCCGCGGATGGGCAGCCGCGCTGGCGGCCCCACCACG
NM_001198557.1      ------------------------------------------------------------
                                                                                

HsCD00598069        ------------------------------------------------------------
NM_005573.3         CCGCTGAGCCCCACGCGCCTGTCGCGGCTCCAGGAGAAGGAGGAGCTGCGCGAGCTCAAT
NM_001198557.1      ------------------------------------------------------------
                                                                                

HsCD00598069        ------------------------------------------------------------
NM_005573.3         GACCGGCTGGCGGTGTACATCGACAAGGTGCGCAGCCTGGAGACGGAGAACAGCGCGCTG
NM_001198557.1      ------------------------------------------------------------
                                                                                

HsCD00598069        ------------------------------------------------------------
NM_005573.3         CAGCTGCAGGTGACGGAGCGCGAGGAGGTGCGCGGCCGTGAGCTCACCGGCCTCAAGGCG
NM_001198557.1      ------------------------------------------------------------
                                                                                

HsCD00598069        ------------------------------------------------------------
NM_005573.3         CTCTACGAGACCGAGCTGGCCGACGCGCGACGCGCGCTCGACGACACGGCCCGCGAGCGC
NM_001198557.1      ------------------------------------------------------------
                                                                                

HsCD00598069        ------------------------------------------------------------
NM_005573.3         GCCAAGCTGCAGATCGAGCTGGGCAAGTGCAAGGCGGAACACGACCAGCTGCTCCTCAAC
NM_001198557.1      ------------------------------------------------------------
                                                                                

HsCD00598069        ------------------------------------------------------------
NM_005573.3         TATGCTAAGAAGGAATCTGATCTTAATGGCGCCCAGATCAAGCTTCGAGAATATGAAGCA
NM_001198557.1      ------------------------------------------------------------
                                                                                

HsCD00598069        ------------------------------------------------------------
NM_005573.3         GCACTGAATTCGAAAGATGCAGCTCTTGCTACTGCACTTGGTGACAAAAAAAGTTTAGAG
NM_001198557.1      ------------------------------------------------------------
                                                                                

HsCD00598069        ------------------------------------------------------------
NM_005573.3         GGAGATTTGGAGGATCTGAAGGATCAGATTGCCCAGTTGGAAGCCTCCTTAGCTGCAGCC
NM_001198557.1      ------------------------------------------------------------
                                                                                

HsCD00598069        ------------------------------------------------------------
NM_005573.3         AAAAAACAGTTAGCAGATGAAACTTTACTTAAAGTAGATTTGGAGAATCGTTGTCAGAGC
NM_001198557.1      ------------------------------------------------------------
                                                                                

HsCD00598069        --------------------------------CCATCACCATCACCATCACAGCCATATG
NM_005573.3         CTTACTGAGGACTTGGAGTTTCGCAAAAGCATGTATGAAGAGGAGATTAACGAGACCAGA
NM_001198557.1      ------------------------------ATGTATGAAGAGGAGATTAACGAGACCAGA
                                                      ** *. *  * .:*.**.. .. * .

HsCD00598069        CTGAGCCCCACGCGCCTGTCGCGGCTCCAGGAGAAGGAGGAGCTGCGCGAGCTCAATGAC
NM_005573.3         AGGAAGCATGAAACGCGCTTGGTAGAGGTGGATTCTGGGCGTCAAATTGAGTATGAGTAC
NM_001198557.1      AGGAAGCATGAAACGCGCTTGGTAGAGGTGGATTCTGGGCGTCAAATTGAGTATGAGTAC
                    . **. *. ....  *  * *  . :  :*** :. *.* . *:..  *** : .*  **

HsCD00598069        CGGCTGGCGGTGTACATCGACAAGGTGCGCAGCCTGGAGACGGAGAACCGCGCGCTGCAG
NM_005573.3         AAGCTGGCGCAAGCCCTTCATGAGATGAGAGAGCAACATGATGCCCAAGTGAGGCTGTAT
NM_001198557.1      AAGCTGGCGCAAGCCCTTCATGAGATGAGAGAGCAACATGATGCCCAAGTGAGGCTGTAT
                    ..******* :. .*.*  * .**.**.*... *:. * .. *. .*.   . **** * 

HsCD00598069        CTGCAGGTGACGGAGCGCGAGGAGGTGCGCGGCCGTGAGCTCACCGGCCTCAAGGCGCTC
NM_005573.3         AAGGAGGAGCTGGAGCAGACTTACCATGCCAAACTTGAGAATGCCAGACTGTCATCAGAG
NM_001198557.1      AAGGAGGAGCTGGAGCAGACTTACCATGCCAAACTTGAGAATGCCAGACTGTCATCAGAG
                    .:* ***:*. *****. ..  *  :   *...* ****.: .**.*.** :.. *. : 

HsCD00598069        TACGAGACCGAGCTGGCCGACG---------------CGCGACGCGCGCTCGACGACACG
NM_005573.3         ATGAATACTTCTACTGTCAACAGTGCCAGGGAAGAACTGATGGAAAGCCGCATGAGAATT
NM_001198557.1      ATGAATACTTCTACTGTCAACAGTGCCAGGGAAGAACTGATGGAAAGCCGCATGAGAATT
                    :: .* **  . .  * *.**.                *. . ...  * *.: ...*  

HsCD00598069        GCCCGCGAGCGCGCCAAGCTGCAGATCGAGCCGGGCAAGTGCAAGGCGGAACACGACCAG
NM_005573.3         GAGAGCCTTTCATCCCAGCTTTCTAATCTACAGAAAGAGTCTAGAGCATGTTTGGAAAGG
NM_001198557.1      GAGAGCCTTTCATCCCAGCTTTCTAATCTACAGAAAGAGTCTAGAGCATGTTTGGAAAGG
                    *. .** :   . **.****  . *:  :.*.*....***  *..**. .: : **...*

HsCD00598069        CTGCTCCTCAACTATGCTAGGAAGGAATCTGATCTTAATGGCGCCCAGATCAAGCTTCGA
NM_005573.3         ATTCAAGAATTAGAGGACTTGCTTGCTAAAGAAAAAGACAACTCTCGTCGCATGCTGACA
NM_001198557.1      ATTCAAGAATTAGAGGACTTGCTTGCTAAAGAAAAAGACAACTCTCGTCGCATGCTGACA
                    .* *:. :.::. * *. : *.: *.::.:**:.::.* ..* * *. . **:*** . *

HsCD00598069        GAATATGAAGCAGCACTGAATTCGAAAGATGCAGCTCTTGCTACTGCACTTGGTGACAAA
NM_005573.3         GACAAAGAGAGAGAGATGGCGGAAATAAGGGATCAAATGCAGCAACAGCTGAATGACTAT
NM_001198557.1      GACAAAGAGAGAGAGATGGCGGAAATAAGGGATCAAATGCAGCAACAGCTGAATGACTAT
                    **.:*:**.. **...**..  ..*:*.. *.: .:.*  . ..: ..** ..****:*:

HsCD00598069        AAAAGTTTAGAGGGAGATTTGGAGGATCTGAAGGATCAGATTG-CCCAGTTGGAAGCCTC
NM_005573.3         GAACAGCTTCTTGATGTAAAGTTAGCCCTGGACATGGAAATCAGTGCTTACAGGAAACTC
NM_001198557.1      GAACAGCTTCTTGATGTAAAGTTAGCCCTGGACATGGAAATCAGTGCTTACAGGAAACTC
                    .**..  *: : *.:*::::* :.*. ***.* .:  *.** .   *: : .*.*..***

HsCD00598069        CTTAGCTGCAGCCAAAAAACAGTTAGCAGATGAAACTTTACTTAAAGTAGATTTGGAGAA
NM_005573.3         TTAGAAGGCGAAGAAGAGAGGTTGAAGCTGTCTCCAAGCCCTTCTTCCCGTGTGACAGTA
NM_001198557.1      TTAGAAGGCGAAGAAGAGAGGTTGAAGCTGTCTCCAAGCCCTTCTTCCCGTGTGACAGTA
                     *:... **... **.*.* . * *. . .* :...:  .***.::  .*: * . **:*

HsCD00598069        TCGTTGTCAGAGCCTTACTGAGGACTTGGAGTTTCGCAAAAGCATGTATGAAGAGGAGAT
NM_005573.3         TCCCGAGCATCCTCAAGTCGTAGTGTACGTACAACTAGAGGAAAGCGGAAGAGGGTTGAT
NM_001198557.1      TCCCGAGCATCCTCAAGTCGTAGTGTACGTACAACTAGAGGAAAGCGGAAGAGGGTTGAT
                    **   . ** .  *::.  *:.*: *: *:. ::* ..*....*   .:..**.* :***

HsCD00598069        TAACGAGACCAGAAGGAAGCATGAAACGCGCTTGGTAGAGGTGGATTCTGGGCGTCAAAT
NM_005573.3         GTGGAAGAATCAGAGGCGAGTAGTAGTGTTAGCATCTCTCATTCCGCCTCAGCCACTGGA
NM_001198557.1      GTGGAAGAATCAGAGGCGAGTAGTAGTGTTAGCATCTCTCATTCCGCCTCAGCCACTGGA
                     :. .***. ...***... ::*:*. *  .  .  : : .*  .  ** .** :*:..:

HsCD00598069        TGAGTATGAGTACAAGCTGGCGCAAGCCCTTCATGAGATGAGAGAGCAACATGATGCCCA
NM_005573.3         AATGTTTGCATCGAAGAAATTGATGTTGATGGGAAATTTATCCGCTTGAAGAACACTTCT
NM_001198557.1      AATGTTTGCATCGAAGAAATTGATGTTGATGGGAAATTTATCCGCTTGAAGAACACTTCT
                    :.:**:**..*. ***.:.  *.:.   .*  .:.* :*.: .*.  .*..:..:   *:

HsCD00598069        AGTGAGGCTGTATAAGGAGGAG-CTGGAGCAGACTTACCATGCCAAACTTGAGAACGCCA
NM_005573.3         GAACAGGATCAACCAATGGGAGGCTGGGAGATGATCAGAAAAATTGGAGACACATCAGTC
NM_001198557.1      GAACAGGATCAACCAATGGGAGGCTGGGAGATGATCAGAAAAATTGGAGACACATCAGTC
                    ..: ***.* :* .*. .**** ****.. * ..* * .*:.. :... : * *:*.  .

HsCD00598069        GACTGTCATCAGAGATGAATACTTCTACTGTCAACAGTGCCAGGGAAGAACTGATGGAAA
NM_005573.3         AGTTATAAATATACCTCAAGATATGTGCTGAAGGCAGGCCAGACTGTTACAATTTGGGCT
NM_001198557.1      AGTTATAAATATACCTCAAGATATGTGCTGAAGGCAGGCCAGACTGTTACAATTTGGGCT
                    .. *.*.*: * * .* ** * :* *.***:...***  *...  .: *..: :***..:

HsCD00598069        GCCGCATGAGAATCGAGAGCCTTTCATCCCAGCTTTCTAATCTACAGAAAGAG--TCTAG
NM_005573.3         GCAAACGCTGGTGTCACAGCCAGCCCCCCAACTGACCTCATCTGGAAGAACCAGAACTCG
NM_001198557.1      GCAAACGCTGGTGTCACAGCCAGCCCCCCAACTGACCTCATCTGGAAGAACCAGAACTCG
                    **....  :*.:   * ****:  *. **.*   : **.****. *..** ..  :**.*

HsCD00598069        AGCATGTTTGGAAAGGATTCAAGAATTAGAGGACTTGCTTGCTAAAGAAAAAGACAACTC
NM_005573.3         TGGGGCACTGGCGAAGATGTGAAGGTTATATTGAAAAATTCTCAGGGAGAGGAGGTTGCT
NM_001198557.1      TGGGGCACTGGCGAAGATGTGAAGGTTATATTGAAAAATTCTCAGGGAGAGGAGGTTGCT
                    :* .  : ***..*.***  .*...*** *  ..::..**   *..**.*.... ::   

HsCD00598069        TCGTCGCATGCTGACAGACAAAGAGAGAGAGATGGCGGAAATAAGGGATCAAATGCAGCA
NM_005573.3         CAAAGAAGTACAGTCTTTAAAACAACCATACCTGAAGAAGAGGAGGAGGAGGAAGAAGCA
NM_001198557.1      CAAAGAAGTACAGTCTTTAAAACAACCATACCTGAAGAAGAGGAGGAGGAGGAAGAAGCA
                     ..: ...*.*:*:*: :.*** *.. * * .**..*.*.* .***.. ...*:*.****

HsCD00598069        ACAG--CTGAATGACTATGAACAGCTTCTTGATGTAAAGTTAGCCCTGGACATGGAAATC
NM_005573.3         GCTGGAGTGGTTGTTGAGGAAGAACTTTTCCACCAGCAGGGAACCCCAAGAGCATCCAAT
NM_001198557.1      GCTGGAGTGGTTGTTGAGGAAGAACTTTTCCACCAGCAGGGAACCCCAAGAGCATCCAAT
                    .*:*   **.:**:  * *** *.*** *  *  :..**  *.*** ..... . ..*: 

HsCD00598069        AGTGCTTACAGGAAACTCTAG
NM_005573.3         AGAAGCTGTGCAATTATGTAA
NM_001198557.1      AGAAGCTGTGCAATTATGTAA
                    **:.  *. . .*::.* **.

Pathways

NCI :

Caspase Cascade in Apoptosis

Panther :

FAS signaling pathway

Reactome :

Apoptosis

Reactome :

Apoptotic cleavage of cellular proteins

Reactome :

Apoptotic execution phase

Reactome :

Breakdown of the nuclear lamina

Reactome :

Cell Cycle

Reactome :

Cell Cycle, Mitotic

Reactome :

Cellular responses to stress

Reactome :

Cellular Senescence

Reactome :

Depolymerisation of the Nuclear Lamina

Reactome :

DNA Damage/Telomere Stress Induced Senescence

Reactome :

Formation of Senescence-Associated Heterochromatin Foci (SAHF)

Reactome :

Initiation of Nuclear Envelope Reformation

Reactome :

M Phase

Reactome :

Meiosis

Reactome :

Meiotic synapsis

Reactome :

Mitotic Anaphase

Reactome :

Mitotic M-M/G1 phases

Reactome :

Mitotic Metaphase and Anaphase

Reactome :

Mitotic Prophase

Reactome :

Nuclear Envelope Breakdown

Reactome :

Nuclear Envelope Reassembly

WikiPathway :

FAS pathway and Stress induction of HSP regulation

Protein

Structural Annotation Wiki :

HR5546C

UniProt :

P20700

HPRD :

01036

Clone

Cloning Information :

628915

Original Clone ID :

HR5546C-22-380-15.9

Experimental

TargetTrack - Experimental data :

HR5546C

Organism

Species Specific ID:

4001

Genome

Chromosome :

Map Location :

5q23.2

Ensembl :

ENSG00000113368

Reagents

Labome :

lamin-B-antibody

LMNB1 (H. sapiens )In pET15_NESG (His-tagged bacterial expression vector)

Coding Sequence Details

Recommended Growth Condition:

Vector Features:

Gene

Reference Sequence Alignment

Pathways

Protein

Clone

Experimental

Organism

Genome

Reagents

LMNB1 (H. sapiens )
In pET15_NESG (His-tagged bacterial expression vector)