DNASU Plasmid | ACTL6A (H. sapiens )<br>In pET15_NESG (His-tagged bacterial expression vector)

Explanation of Terms

Gene:

Gene Symbol: ACTL6A

Gene Name: actin-like 6A

Original Clone ID:

HR2893D-1-190-15.2

Keyword:

targeted domain

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pET15_NESG

Format:

CLOSED

Source:

Northeast Structural Genomics Consortium

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Rutgers University
PSI:Biology
Northeast Structural Genomics Consortium

HsCD00598297

Price:

AVAILABLE

No restriction
Special MTA: PSI/NESG

Insert Sequence
Protein Sequence

Insert sequence: 573nts

Open reading frame: 51 to 623

Reference Sequence Alignment

CTTTAAGAAGGAGATATACCATGGGCCATCACCATCACCATCACAGCCATATGAGCGGCG
GCGTGTACGGGGGAGATGAAGTTGGAGCCCTTGTTTTTGACATTGGATCCTATACTGTGA
GAGCTGGTTATGCTGGTGAGGACTGCCCCAAGGTGGATTTTCCTACAGCTATTGGTATGG
TGGTAGAAAGAGATGACGGAAGCACATTAATGGAAATAGATGGCGATAAAGGCAAACAAG
GCGGTCCCACCTACTACATAGATACTAATGCTCTGCGTGTTCCGAGGGAGAATATGGAGG
CCATTTCACCTCTAAAAAATGGGATGGTTGAAGACTGGGATAGTTTCCAAGCTATTTTGG
ATCATACCTACAAAATGCATGTCAAATCAGAAGCCAGTCTCCATCCTGTTCTCATGTCAG
AGGCACCGTGGAATACTAGAGCAAAGAGAGAGAAACTGACAGAGTTAATGTTTGAACACT
ACAACATCCCTGCCTTCTTCCTTTGCAAAACTGCAGTTTTGACAGCATTTGCTAATGGTC
GTTCTACTGGGCTGATTTTGGACAGTGGAGCCACTCATACCACTGCAATTCCAGTCCACG
ATGGCTATGTCCTTCAACAATAGCTCGAGATCCGGCTGCTAACAAAGCCCGAAAGGAAGC
TGAGTTGGCTGCTG

Protein sequence predicted based on nucleotide sequence

MSGGVYGGDEVGALVFDIGSYTVRAGYAGEDCPKVDFPTAIGMVVERDDGSTLMEIDGDK
GKQGGPTYYIDTNALRVPRENMEAISPLKNGMVEDWDSFQAILDHTYKMHVKSEASLHPV
LMSEAPWNTRAKREKLTELMFEHYNIPAFFLCKTAVLTAFANGRSTGLILDSGATHTTAI
PVHDGYVLQQ*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

ProteinExpressed: Protein_Confirmed

ProteinPurified: Protein_Purified

SolubleProtein: Protein_Soluble

Find experimental protocols in HR2893D

Recommended expression in:

Not Applicable

Vector Name:

pET15_NESG

Synonyms:

pET15, pET15-modified, pET15Cc, pET15_NESG

Sequencing Primer:

Forward: T7

Reverse: T7 terminator

Description:

Bacterial (E. coli) expression vector with T7 lac promoter, adds N-terminal 6x His tag; amp resistance; restriction enzyme cloning.

Comments:

Modified Novagen pET vector-common MCS

Size (bp):

5723

Parent Vector:

None

Properties:

bacterial expression, in vitro transcription, multiple cloning site, with tag/fusion/marker

Author Name:

Gaetano T. Montleione
Jessica Bearden
Northeast Structural Genomics Consortium

Publications:

PMID: 15808222

Title: Robotic cloning and Protein Production Platform of the Northeast Structural Genomics Consortium

Vector Map:

Vector Sequence:

Sequence:

TTCTCATGTTTGACAGCTTATCATCGATAAGCTAGCTTTAATGCGGTAGTTTATCACAGTTAAATTGCTAACGCAGTCAGGCACCGTGTATGAAATCTAACAATGCGCTCATCGTCATCCTCGGCACCGTCACCCTGGATGCTGTAGGCATAGGCTTGGTTATGCCGGTACTGCCGGGCCTCTTGCGGGATATCCGGATATAGTTCCTCCTTTCAGCAAAAAACCCCTCAAGACCCGTTTAGAGGCCCCAAGGGGTTATGCTAGTTATTGCTCAGCGGTGGCAGCAGCCAACTCAGCTTCCTTTCGGGCTTTGTTAGCAGCCGGATCTCGAGAAGCTTTGCGTCGACAGAGCTCGCAGGATCCGCAGAATTCGCCATATGGCTGTGATGGTGATGGTGATGGCCCATGGTATATCTCCTTCTTAAAGTTAAACAAAATTATTTCTAGAGGGGAATTGTTATCCGCTCACAATTCCCCTATAGTGAGTCGTATTAATTTCGCGGGATCGAGATCTCGATCCTCTACGCCGGACGCATCGTGGCCGGCATCACCGGCGCCACAGGTGCGGTTGCTGGCGCCTATATCGCCGACATCACCGATGGGGAAGATCGGGCTCGCCACTTCGGGCTCATGAGCGCTTGTTTCGGCGTGGGTATGGTGGCAGGCCCCGTGGCCGGGGGACTGTTGGGCGCCATCTCCTTGCATGCACCATTCCTTGCGGCGGCGGTGCTCAACGGCCTCAACCTACTACTGGGCTGCTTCCTAATGCAGGAGTCGCATAAGGGAGAGCGTCGAGATCCCGGACACCATCGAATGGCGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAATGTGAAACCAGTAACGTTATACGATGTCGCAGAGTATGCCGGTGTCTCTTATCAGACCGTTTCCCGCGTGGTGAACCAGGCCAGCCACGTTTCTGCGAAAACGCGGGAAAAAGTGGAAGCGGCGATGGCGGAGCTGAATTACATTCCCAACCGCGTGGCACAACAACTGGCGGGCAAACAGTCGTTGCTGATTGGCGTTGCCACCTCCAGTCTGGCCCTGCACGCGCCGTCGCAAATTGTCGCGGCGATTAAATCTCGCGCCGATCAACTGGGTGCCAGCGTGGTGGTGTCGATGGTAGAACGAAGCGGCGTCGAAGCCTGTAAAGCGGCGGTGCACAATCTTCTCGCGCAACGCGTCAGTGGGCTGATCATTAACTATCCGCTGGATGACCAGGATGCCATTGCTGTGGAAGCTGCCTGCACTAATGTTCCGGCGTTATTTCTTGATGTCTCTGACCAGACACCCATCAACAGTATTATTTTCTCCCATGAAGACGGTACGCGACTGGGCGTGGAGCATCTGGTCGCATTGGGTCACCAGCAAATCGCGCTGTTAGCGGGCCCATTAAGTTCTGTCTCGGCGCGTCTGCGTCTGGCTGGCTGGCATAAATATCTCACTCGCAATCAAATTCAGCCGATAGCGGAACGGGAAGGCGACTGGAGTGCCATGTCCGGTTTTCAACAAACCATGCAAATGCTGAATGAGGGCATCGTTCCCACTGCGATGCTGGTTGCCAACGATCAGATGGCGCTGGGCGCAATGCGCGCCATTACCGAGTCCGGGCTGCGCGTTGGTGCGGATATCTCGGTAGTGGGATACGACGATACCGAAGACAGCTCATGTTATATCCCGCCGTTAACCACCATCAAACAGGATTTTCGCCTGCTGGGGCAAACCAGCGTGGACCGCTTGCTGCAACTCTCTCAGGGCCAGGCGGTGAAGGGCAATCAGCTGTTGCCCGTCTCACTGGTGAAAAGAAAAACCACCCTGGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTAAGTTAGCTCACTCATTAGGCACCGGGATCTCGACCGATGCCCTTGAGAGCCTTCAACCCAGTCAGCTCCTTCCGGTGGGCGCGGGGCATGACTATCGTCGCCGCACTTATGACTGTCTTCTTTATCATGCAACTCGTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGCGCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCCTTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATGGCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTCCCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTGTCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACCAGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGCCTCGGCGAGCACATGGAACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGTCGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACGGATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAACCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCATCTCGGGCAGCGTTGGGTCCTGGCCACGGGTGCGCATGATCGTGCTCCTGTCGTTGAGGACCCGGCTAGGCTGGCGGGGTTGCCTTACTGGTTAGCAGAATGAATCACCGATACGCGAGCGAACGTGAAGCGACTGCTGCTGCAAAACGTCTGCGACCTGAGCAACAACATGAATGGTCTTCGGTTTCCGTGTTTCGTAAAGTCTGGAAACGCGGAAGTCAGCGCCCTGCACCATTATGTTCCGGATCTGCATCGCAGGATGCTGCTGGCTACCCTGTGGAACACCTACATCTGTATTAACGAAGCGCTGGCATTGACCCTGAGTGATTTTTCTCTGGTCCCGCCGCATCCATACCGCCAGTTGTTTACCCTCACAACGTTCCAGTAACCGGGCATGTTCATCATCAGTAACCCGTATCGTGAGCATCCTCTCTCGTTTCATCGGTATCATTACCCCCATGAACAGAAATCCCCCTTACACGGAGGCATCAGTGACCAAACAGGAAAAAACCGCCCTTAACATGGCCCGCTTTATCAGAAGCCAGACATTAACGCTTCTGGAGAAACTCAACGAGCTGGACGCGGATGAACAGGCAGACATCTGTGAATCGCTTCACGACCACGCTGATGAGCTTTACCGCAGCTGCCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTGCAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAACACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTTCAAGAA

Vector Features:

Type

Name

Description

Start

End

MCS

MCS (includes SphI, XbaI, EcoRI, BamHI, XhoI)

327

452

bacterial origin

ori

ColE1 origin of replication

3881

4563

gene fragment

ROP

repressor of primer ROP sequence

3281

3457

primer

T7 forward

T7 forward sequencing primer

481

496

primer

T7 reverse

T7 reverse sequencing primer

257

275

promoter

T7 promoter

478

497

repressor binding site

lacO

lacO regulatory sequence

456

478

selectable marker

AmpR

ampicillin resistance gene

4661

5230

tag

His

N-terminal 6x His tag

387

404

trxn termination sequence

T7 term

T7 termination sequence

257

275

Gene

Gene Symbol :

ACTL6A

Symbol Nomenclature :

ACTL6A

SYNONYM :

ACTL6; ARPN-BETA; Arp4; BAF53A; INO80K

Designation :

Full Nomenclature :

actin-like 6A

GENEID :

GI :

30089999

GenBank Accession :

NM_178042

HGNC :

24124

MIM :

604958

Vega :

OTTHUMG00000157782

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00598297      CTTTAAGAAGGAGATATACCATGGGCCATCACCATCACCATCACAGCCATATGAGCGGCG
NM_004301.3       --------------------------------------------------ATGAGCGGCG
NM_178042.2       ------------------------------------------------------------
NM_177989.2       ------------------------------------------------------------
                                                                              

HsCD00598297      GCGTGTACGGGGGAGATGAAGTTGGAGCCCTTGTTTTTGACATTGGATCCTATACTGTGA
NM_004301.3       GCGTGTACGGGGGAGATGAAGTTGGAGCCCTTGTTTTTGACATTGGATCCTATACTGTGA
NM_178042.2       ------------------------------------------------------------
NM_177989.2       ------------------------------------------------------------
                                                                              

HsCD00598297      GAGCTGGTTATGCTGGTGAGGACTGCCCCAAGGTGGATTTTCCTACAGCTATTGGTATGG
NM_004301.3       GAGCTGGTTATGCTGGTGAGGACTGCCCCAAGGTGGATTTTCCTACAGCTATTGGTATGG
NM_178042.2       --------------------------------------------------------ATGG
NM_177989.2       --------------------------------------------------------ATGG
                                                                          ****

HsCD00598297      TGGTAGAAAGAGATGACGGAAGCACATTAATGGAAATAGATGGCGATAAAGGCAAACAAG
NM_004301.3       TGGTAGAAAGAGATGACGGAAGCACATTAATGGAAATAGATGGCGATAAAGGCAAACAAG
NM_178042.2       TGGTAGAAAGAGATGACGGAAGCACATTAATGGAAATAGATGGCGATAAAGGCAAACAAG
NM_177989.2       TGGTAGAAAGAGATGACGGAAGCACATTAATGGAAATAGATGGCGATAAAGGCAAACAAG
                  ************************************************************

HsCD00598297      GCGGTCCCACCTACTACATAGATACTAATGCTCTGCGTGTTCCGAGGGAGAATATGGAGG
NM_004301.3       GCGGTCCCACCTACTACATAGATACTAATGCTCTGCGTGTTCCGAGGGAGAATATGGAGG
NM_178042.2       GCGGTCCCACCTACTACATAGATACTAATGCTCTGCGTGTTCCGAGGGAGAATATGGAGG
NM_177989.2       GCGGTCCCACCTACTACATAGATACTAATGCTCTGCGTGTTCCGAGGGAGAATATGGAGG
                  ************************************************************

HsCD00598297      CCATTTCACCTCTAAAAAATGGGATGGTTGAAGACTGGGATAGTTTCCAAGCTATTTTGG
NM_004301.3       CCATTTCACCTCTAAAAAATGGGATGGTTGAAGACTGGGATAGTTTCCAAGCTATTTTGG
NM_178042.2       CCATTTCACCTCTAAAAAATGGGATGGTTGAAGACTGGGATAGTTTCCAAGCTATTTTGG
NM_177989.2       CCATTTCACCTCTAAAAAATGGGATGGTTGAAGACTGGGATAGTTTCCAAGCTATTTTGG
                  ************************************************************

HsCD00598297      ATCATACCTACAAAATGCATGTCAAATCAGAAGCCAGTCTCCATCCTGTTCTCATGTCAG
NM_004301.3       ATCATACCTACAAAATGCATGTCAAATCAGAAGCCAGTCTCCATCCTGTTCTCATGTCAG
NM_178042.2       ATCATACCTACAAAATGCATGTCAAATCAGAAGCCAGTCTCCATCCTGTTCTCATGTCAG
NM_177989.2       ATCATACCTACAAAATGCATGTCAAATCAGAAGCCAGTCTCCATCCTGTTCTCATGTCAG
                  ************************************************************

HsCD00598297      AGGCACCGTGGAATACTAGAGCAAAGAGAGAGAAACTGACAGAGTTAATGTTTGAACACT
NM_004301.3       AGGCACCGTGGAATACTAGAGCAAAGAGAGAGAAACTGACAGAGTTAATGTTTGAACACT
NM_178042.2       AGGCACCGTGGAATACTAGAGCAAAGAGAGAGAAACTGACAGAGTTAATGTTTGAACACT
NM_177989.2       AGGCACCGTGGAATACTAGAGCAAAGAGAGAGAAACTGACAGAGTTAATGTTTGAACACT
                  ************************************************************

HsCD00598297      ACAACATCCCTGCCTTCTTCCTTTGCAAAACTGCAGTTTTGACAGCATTTGCTAATGGTC
NM_004301.3       ACAACATCCCTGCCTTCTTCCTTTGCAAAACTGCAGTTTTGACAGCATTTGCTAATGGTC
NM_178042.2       ACAACATCCCTGCCTTCTTCCTTTGCAAAACTGCAGTTTTGACAGCATTTGCTAATGGTC
NM_177989.2       ACAACATCCCTGCCTTCTTCCTTTGCAAAACTGCAGTTTTGACAGCATTTGCTAATGGTC
                  ************************************************************

HsCD00598297      GTTCTACTGGGCTGATTTTGGACAGTGGAGCCACTCATACCACTGCAATTCCAGTCCACG
NM_004301.3       GTTCTACTGGGCTGATTTTGGACAGTGGAGCCACTCATACCACTGCAATTCCAGTCCACG
NM_178042.2       GTTCTACTGGGCTGATTTTGGACAGTGGAGCCACTCATACCACTGCAATTCCAGTCCACG
NM_177989.2       GTTCTACTGGGCTGATTTTGGACAGTGGAGCCACTCATACCACTGCAATTCCAGTCCACG
                  ************************************************************

HsCD00598297      ATGGCTATGTCCTTCAACAATAGCTCGAGATCCGGCTGCTAACAAAGCCCGAAAGGAAGC
NM_004301.3       ATGGCTATGTCCTTCAACAAGGCATTGTGAAATCCCCTCTTGCTGGAGACTTTATTACTA
NM_178042.2       ATGGCTATGTCCTTCAACAAGGCATTGTGAAATCCCCTCTTGCTGGAGACTTTATTACTA
NM_177989.2       ATGGCTATGTCCTTCAACAAGGCATTGTGAAATCCCCTCTTGCTGGAGACTTTATTACTA
                  ******************** . .* *:**:.   *  **:.*:... .* ::*  *. .

HsCD00598297      TGAGTTGGCTGCTG----------------------------------------------
NM_004301.3       TGCAGTGCAGAGAACTCTTCCAAGAAATGAATATTGAATTGGTTCCTCCATATATGATTG
NM_178042.2       TGCAGTGCAGAGAACTCTTCCAAGAAATGAATATTGAATTGGTTCCTCCATATATGATTG
NM_177989.2       TGCAGTGCAGAGAACTCTTCCAAGAAATGAATATTGAATTGGTTCCTCCATATATGATTG
                  **.. ** . . :.                                              

HsCD00598297      ------------------------------------------------------------
NM_004301.3       CATCAAAAGAAGCTGTTCGTGAAGGATCTCCAGCAAACTGGAAAAGAAAAGAGAAGTTGC
NM_178042.2       CATCAAAAGAAGCTGTTCGTGAAGGATCTCCAGCAAACTGGAAAAGAAAAGAGAAGTTGC
NM_177989.2       CATCAAAAGAAGCTGTTCGTGAAGGATCTCCAGCAAACTGGAAAAGAAAAGAGAAGTTGC
                                                                              

HsCD00598297      ------------------------------------------------------------
NM_004301.3       CTCAGGTTACGAGGTCTTGGCACAATTATATGTGTAATTGTGTTATCCAGGATTTTCAAG
NM_178042.2       CTCAGGTTACGAGGTCTTGGCACAATTATATGTGTAATTGTGTTATCCAGGATTTTCAAG
NM_177989.2       CTCAGGTTACGAGGTCTTGGCACAATTATATGTGTAATTGTGTTATCCAGGATTTTCAAG
                                                                              

HsCD00598297      ------------------------------------------------------------
NM_004301.3       CTTCGGTACTTCAAGTGTCAGATTCAACTTATGATGAACAAGTGGCTGCACAGATGCCAA
NM_178042.2       CTTCGGTACTTCAAGTGTCAGATTCAACTTATGATGAACAAGTGGCTGCACAGATGCCAA
NM_177989.2       CTTCGGTACTTCAAGTGTCAGATTCAACTTATGATGAACAAGTGGCTGCACAGATGCCAA
                                                                              

HsCD00598297      ------------------------------------------------------------
NM_004301.3       CTGTTCATTATGAATTCCCCAATGGCTACAATTGTGATTTTGGTGCAGAGCGGCTAAAGA
NM_178042.2       CTGTTCATTATGAATTCCCCAATGGCTACAATTGTGATTTTGGTGCAGAGCGGCTAAAGA
NM_177989.2       CTGTTCATTATGAATTCCCCAATGGCTACAATTGTGATTTTGGTGCAGAGCGGCTAAAGA
                                                                              

HsCD00598297      ------------------------------------------------------------
NM_004301.3       TTCCAGAAGGATTATTTGACCCTTCCAATGTAAAGGGGTTATCAGGAAACACAATGTTAG
NM_178042.2       TTCCAGAAGGATTATTTGACCCTTCCAATGTAAAGGGGTTATCAGGAAACACAATGTTAG
NM_177989.2       TTCCAGAAGGATTATTTGACCCTTCCAATGTAAAGGGGTTATCAGGAAACACAATGTTAG
                                                                              

HsCD00598297      ------------------------------------------------------------
NM_004301.3       GAGTCAGTCATGTTGTCACCACAAGTGTTGGGATGTGTGATATTGACATCAGACCAGGTC
NM_178042.2       GAGTCAGTCATGTTGTCACCACAAGTGTTGGGATGTGTGATATTGACATCAGACCAGGTC
NM_177989.2       GAGTCAGTCATGTTGTCACCACAAGTGTTGGGATGTGTGATATTGACATCAGACCAGGTC
                                                                              

HsCD00598297      ------------------------------------------------------------
NM_004301.3       TCTATGGCAGTGTAATAGTGGCAGGAGGAAACACACTAATACAGAGTTTTACTGACAGGT
NM_178042.2       TCTATGGCAGTGTAATAGTGGCAGGAGGAAACACACTAATACAGAGTTTTACTGACAGGT
NM_177989.2       TCTATGGCAGTGTAATAGTGGCAGGAGGAAACACACTAATACAGAGTTTTACTGACAGGT
                                                                              

HsCD00598297      ------------------------------------------------------------
NM_004301.3       TGAATAGAGAGCTGTCTCAGAAAACTCCTCCAAGTATGCGGTTGAAATTGATTGCAAATA
NM_178042.2       TGAATAGAGAGCTGTCTCAGAAAACTCCTCCAAGTATGCGGTTGAAATTGATTGCAAATA
NM_177989.2       TGAATAGAGAGCTGTCTCAGAAAACTCCTCCAAGTATGCGGTTGAAATTGATTGCAAATA
                                                                              

HsCD00598297      ------------------------------------------------------------
NM_004301.3       ATACAACAGTGGAACGGAGGTTTAGCTCATGGATTGGCGGCTCCATTCTAGCCTCTTTGG
NM_178042.2       ATACAACAGTGGAACGGAGGTTTAGCTCATGGATTGGCGGCTCCATTCTAGCCTCTTTGG
NM_177989.2       ATACAACAGTGGAACGGAGGTTTAGCTCATGGATTGGCGGCTCCATTCTAGCCTCTTTGG
                                                                              

HsCD00598297      ------------------------------------------------------------
NM_004301.3       GTACCTTTCAACAGATGTGGATTTCCAAGCAAGAATATGAAGAAGGAGGGAAGCAGTGTG
NM_178042.2       GTACCTTTCAACAGATGTGGATTTCCAAGCAAGAATATGAAGAAGGAGGGAAGCAGTGTG
NM_177989.2       GTACCTTTCAACAGATGTGGATTTCCAAGCAAGAATATGAAGAAGGAGGGAAGCAGTGTG
                                                                              

HsCD00598297      --------------------
NM_004301.3       TAGAAAGAAAATGCCCTTGA
NM_178042.2       TAGAAAGAAAATGCCCTTGA
NM_177989.2       TAGAAAGAAAATGCCCTTGA

Pathways

NCI :

C-MYC pathway

NCI :

Validated targets of C-MYC transcriptional activation

Reactome :

Chromatin modifying enzymes

Reactome :

Chromatin organization

Reactome :

HATs acetylate histones

Protein

Structural Annotation Wiki :

HR2893D

UniProt :

O96019

HPRD :

05389

Clone

Cloning Information :

625983

Original Clone ID :

HR2893D-1-190-15.2

Experimental

TargetTrack - Experimental data :

HR2893D

Organism

Species Specific ID:

Genome

Chromosome :

Map Location :

3q26.33

Ensembl :

ENSG00000136518

Reagents

Labome :

ACTL6A-antibody

ACTL6A (H. sapiens )In pET15_NESG (His-tagged bacterial expression vector)

Coding Sequence Details

Recommended Growth Condition:

Vector Features:

Gene

Reference Sequence Alignment

Pathways

Protein

Clone

Experimental

Organism

Genome

Reagents

ACTL6A (H. sapiens )
In pET15_NESG (His-tagged bacterial expression vector)