Gene:
Gene Symbol:
EREG
Gene Name:
epiregulin
Original Clone ID:
HsCD00301616
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pANT7_cGST
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
Identical to HsCD00730573
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00603338
Price:
Login for Pricing
UNAVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 507nts
Open reading frame: 167 to 673
TCACTTGCCTGATATTTCCAGTGTCAGAGGGACACAGCCAACGTGGGGTCCCTTCTAGGC
TGACAGCCGCTCTCCAGCCACTGCCGCGAGCCCGTCTGCTCCCGCCCTGCCCGTGCACTC
TCCGCAGCCGCCCTCCGCCAAGCCCCAGCGCCCGCTCCCATCGCCGATGACCGCGGGGAG
GAGGATGGAGATGCTCTGTGCCGGCAGGGTCCCTGCGCTGCTGCTCTGCCTGGGTTTCCA
TCTTCTACAGGCAGTCCTCAGTACAACTGTGATTCCATCATGTATCCCAGGAGAGTCCAG
TGATAACTGCACAGCTTTAGTTCAGACAGAAGACAATCCACGTGTGGCTCAAGTGTCAAT
AACAAAGTGTAGCTCTGACATGAATGGCTATTGTTTGCATGGACAGTGCATCTATCTGGT
GGACATGAGTCAAAACTACTGCAGGTGTGAAGTGGGTTATACTGGTGTCCGATGTGAACA
CTTCTTTTTAACCGTCCACCAACCTTTAAGCAAAGAGTATGTGGCTTTGACCGTGATTCT
TATTATTTTGTTTCTTATCACAGTCGTCGGTTCCACATATTATTTCTGCAGATGGTACAG
AAATCGAAAAAGTAAAGAACCAAAGAAGGAATATGAGAGAGTTACCTCAGGGGATCCAGA
GTTGCCGCAAGTCTGAATGGCGCCATCAAACTTATGGGCAGGGATAACAGTGTGCCTGGT
TAATATTAATATTCCATTTTATTAATAATATTTATGTTGGGTCAAGTGTTAGGTCAATAA
CACTGTATTTTAATGTACTTGAAAAATGTTTTTATTTTTGTTTTATTTTTGACAGACTAT
TTGCTAATGTATAATGTGCAGAAAATATTTAATATCAAAAGAAAATTGATATTTTTATAC
AAGTAATTTCCTGAGCTAAATGCTTCATTGAAAGCTTCAAAGTTTATATGCCTGGTGCAC
AGTGCTTAGAAGTAAGCAATTCCCAGGTCATAGCTCAAGAATTGTTAGCAAATGACAGAT
TTCTGTAAGCCTATATATATAGTCAAATCGATTTAGTAAGTATGTTTTTTATGTTCCTCA
AATCAGTGATAATTGGTTTGACTGTACCATGGTTTGATATGTAGTTGGCACCATGGTATC
ATATATTAAAACAATAATGCAATTAGAATTTGGGAGAAGCAAATATAGGTCCTGTGTTAA
ACACTACACATTTGAAACAAGCTAACCCTGGGGAGTCTATGGTCTCTTCACTCAGGTCTC
AGCTATAATTCTGTTATATGAGGGGCAGTGGACAGTTCCCTATGCCAACTCACGACTCCT
ACAGGTACTAGTCACTCATCTACCAGATTCTGCCTATGTAAAATGAATTGAAAAACAATT
TTCTGTAATCTTTTATTTAAGTAGTGGGCATTTCATAGCTTCACAATGTTCCTTTTTTGT
ATATTACAACATTTATGTGAGGTAATTATTGCTCAACAGACAATTAGAAAAAAGTCCACA
CTTGAAGCCTAAATTTGTGCTTTTTAAGAATATTTTTAGACTATTTCTTTTTATAGGGGC
TTTGCTGAATTCTAACATTAAATCACAGCCCAAAATTTGATGGACTAATTATTATTTTAA
AATATATGAAGACAATAATTCTACATGTTGTCTTAAGATGGAAATACAGTTATTTCATCT
TTTATTCAAGGAAGTTTTAACTTTAATACAGCTCAGTAAATGGCTTCTTCTAGAATGTAA
AGTTATGTATTTAAAGTTGTATCTTGACACAGGAAATGGGAAAAAACTTAAAAATTAATA
TGGTGTATTTTTCCAAATGAAAAATCTCAATTGAAAGCTTTTAAAATGTAGAAACTTAAA
CACACCTTCCTGTGGAGGCTGAGATGAAAACTAGGGCTCATTTTCCTGACATTTGTTTAT
TTTTTGGAAGAGACAAAGATTTCTTCTGCACTCTGAGCCCATAGGTCTCAGAGAGTTAAT
AGGAGTATTTTTGGGCTATTGCATAAGGAGCCACTGCTGCCACCACTTTTGGATTTTATG
GGAGGCTCCTTCATCGAATGCTAAACCTTTGAGTAGAGTCTCCCTGGATCACATACCAGG
TCAGGGAGGATCTGTTCTTCCTCTACGTTTATCCTGGCATGTGCTAGGGTAAACGAAGGC
ATAATAAGCCATGGCTGACCTCTGGAGCACCAGGTGCCAGGACTTGTCTCCATGTGTATC
CATGCATTATATACCCTGGTGCAATCACACGACTGTCATCTAAAGTCCTGGCCCTGGCCC
TTACTATTAGGAAAATAAACAGACAAAAACAAGTAAATATATATGGTCCTATACATATTG
TATATATATTCATATACAAACATGTATGTATACATGACCTTAATGGATCATAGAATTGCA
GTCATTTGGTGCTCTGCTAACCATTTATATAAAACTTAAAAACAAGAGAAAAGAAAAATC
AATTAGATCTAAACAGTTATTTCTGTTTCCTATTTAATATAGCTGAAGTCAAAATATGTA
AGAACACATTTTAAATACTCTACTTACAGTTGGCCCTCTGTGGTTAGTTCCACATCTGTG
GATTCAACCAACCAAGGACGGAAAATGCTTAAAAAATAATACAACAACAACAAAAAATAC
ATTATAACAACTATTTACTTTTTTTTTTTTCTTTTTGAGATGGAGTCTCGCTCTGTTGCC
CAGGTTGGAGTGCAGTGGCACGATCTCGGCTCACTGCAACCTCACCTCCCGGGTTCAAGA
GATCCTCCTGCCTCAGCCTCCTGAGCAGCTGGGACTACAGGCGCATGCCACCATGCCCAG
CTAATTTTTGTATTTTTAGTAGAGGCGGGGTTTCACCATGTTGGCCAGGATGGTCTCAAT
CTCCTAACCTTGAGATCCACCCTCCACAGCCTCCCAAACTGCTGGGATTACAGGCGTGAG
CCACCGCACGTAGCATTTACATTAGGTATTACAAGTAATGTAAAGATGATTTAAGTATAC
AGGAGGATGTGAATAGGTTATATGCAAGCACTATGCCCTTTTATATAAGTGACTTGAACA
TCTGTGCCCGATTTTAGTATGTGCAGGGGGGCGATCTGGGAATCAGTCCCCTGTGGATAC
CAAGGTACAACTGTATTTATTAACGCTTACTAGATGTGAGGAGAGTCTGAATATTTTCAG
TGATCTTGGCTGTTTCAAAAAAATCTATTGACTTTTCAATAAATCAGCTGCAATCCATTT
ATTTCATTTACAAAAGATTTATTGTAAGCCTCTCAATCTTGGTTTTTCAGTTGATCTTAA
GCATGTCAATTCATAAAAACAAGTCATTTTTGTATTTTTCATCTTTAAGAATGCTTAAAA
AAGCTAATCCCTAAAATAGTTAGATCTTTGTAAATGCATATTAAATAATAAAGTATGACC
CACATTACTTTTTATGGGTGAAAATAAGACAAAAATAATAGTTTTAGTGAGGATGGTGCT
GAGTAAACATAAAAACTGATTTGCTCTCAGCTGATGTGTCCTGTACACAGTGGGAAGATT
TTAGTTCACACTTAGTCTAACTCCCCCATTTTACAGATTTCTCACTATATATATTTCTAG
AAGGGGCTATGCATATTCAATGTATTGAGAACCAAAGCAACCACAAATGCATAAATGCAT
AATTTATGGTCTTCAACCAAGGCCACATAATAACCCAGTTAACTTACTCTTTAACCAGGA
ATATTAAGTTCTATAACTAGTACTCAAGGTTTAACCTTAAAATTAAGATTTCCTTAACCT
TAACCTTAAAATTGATATTATATTAAACATACATAATACAATGTAACTCCACTGTTCTCC
TGAATATTTTTTGCTCTAATCTCTCTGCCGAAAGTCAAAGTGATGGGAGAATTGGTATAC
TGGTATGACTACGTCTTAAGTCAGATTTTTATTTATGAGTCTTTGAGACTAAATTCAATC
ACCACCAGGTATCAAATCAACTTTTATGCAGCAAATATATGATTCTAGTGTCTGACTTTT
GTTAAATTCAGTAATGCAGTTTTTAAAAACCTGTATCTGACCCACTTTGTAATTTTTGCT
CCAATATCCATTCTGTAGACTTTTGAAAAAAAAGTTTTTAATTTGATGCCCAATATATTC
TGACCGTTAAAAAATTCTTGTTCATATGGGAGAAGGGGGAGTAATGACTTGTACAAACAG
TATTTCTGGTGTATATTTTAATGTTTTTAAAAAGAGTAATTTCATTTAAATATCTGTTAT
TCAAATTTGATGATGTTAAATGTAATATAATGTATTTTCTTTTTATTTTGCACTCTGTAA
TTGCACTTTTTAAGTTTGAAGAGCCATTTTGGTAAACGGTTTTTATTAAAGATGCTATGG
AACATAAAGTTGTATTGCATGCAATTTAAAGTAACTTATTTGACTATGAATATTATCGGA
TTACTGAATTGTATCAATTTGTTTGTGTTCAATATCAGCTTTGATAATTGTGTACCTTAA
GATATTGAAGGAGAAAATAGATAATTTACAAGATATTATTAATTTTTATTTATTTTTCTT
GGGAATTGAAAAAAATTGAAATAAATAAAAATGCATTGAACATCTTGCATTCAAAATCTT
CACTGAC
Protein sequence predicted based on nucleotide sequence
MTAGRRMEMLCAGRVPALLLCLGFHLLQAVLSTTVIPSCIPGESSDNCTALVQTEDNPRV AQVSITKCSSDMNGYCLHGQCIYLVDMSQNYCRCEVGYTGVRCEHFFLTVHQPLSKEYVA LTVILIILFLITVVGSTYYFCRWYRNRKSKEPKKEYERVTSGDPELPQV
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pANT7_cGST
Synonyms:
pANT7-cGST
Description:
with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial
Comments:
One in a series of pANT7 vectors made by A. Lau and N. Ramachandran for use with NAPPA arrays. Can be used for cell free expression of the gene insert using human IVTT from Thermo Fisher or rabbit reticulocyte systems
Size (bp):
5964
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Joshua LaBaer
Al Lau
Niroshan Ramachandran
Al Lau
Niroshan Ramachandran
Publications:
PMID:
15232106
Title: Self-assembling protein microarrays
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 bacterial origin of replication
3931
3249
death cassette
ccdB
ccdB death cassette present in 'empty vector' form only (replace with gene of interest)
1876
2085
primer
NAP138
NAP138 reverse sequencing primer 5? TGTTTCGCCATTTATCACCTTC 3?
2405
2384
primer
NAP150
Forward sequencing primer NAP150 5?-CCC ATT GTA TGG GAT CTG ATC-3?
388
408
promoter
T7
T7 transcriptional start sequence
5940
5964
recombination site
attR1
AttR1 site for recombination in empty vector
549
565
recombination site
attR2
AttR2 site for recombination in empty vector
2236
2251
selectable marker
CmR
chloramphenicol resistance
782
1438
selectable marker
AmpR
ampicillin resistance gene
4688
4029
tag
GST
Glutathione transferase (GST) tag
2282
2950
