DNASU Plasmid | MOCS3 (H. sapiens )<br>In pANT7_cGST (GST-tagged<br>In vitro expression vector)

Explanation of Terms

Gene:

Gene Symbol: UTY

Gene Name: ubiquitously transcribed tetratricopeptide repeat containing, Y-linked

Original Clone ID:

HsCD00078426

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pANT7_cGST

Format:

FUSION

Source:

Center for Personalized Diagnostics

Description:

None

Comments:

Identical to HsCD00731431

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Center for Personalized Diagnostics

HsCD00603863

Price:

UNAVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1380nts

Open reading frame: 30 to 1409

Reference Sequence Alignment

CGGACGCGTGGGTTCCGGAAGAGGTCGCCATGGCTTCCCGGGAGGAGGTACTCGCCTTAC
AAGCTGAAGTTGCCCAACGTGAGGAGGAATTGAATTCGCTGAAGCAGAAGCTGGCGTCGG
CTCTTTTGGCTGAGCAGGAACCGCAGCCAGAACGGCTGGTTCCGGTGTCGCCGCTGCCGC
CGAAGGCCGCTCTGTCCCGAGATGAGATTCTGCGCTATAGCCGGCAGCTAGTGCTGCCCG
AGCTGGGCGTGCACGGACAGCTGCGCCTGGGGACCGCGTGCGTGCTAATCGTGGGCTGCG
GTGGACTCGGCTGTCCACTAGCGCAGTACTTGGCAGCGGCCGGCGTGGGCCGCCTTGGCC
TTGTGGACTATGACGTGGTAGAGATGAGCAACCTGGCCCGCCAAGTGCTGCATGGCGAGG
CACTGGCTGGCCAGGCCAAGGCCTTTTCGGCCGCCGCCTCGCTGCGCCGCCTCAATTCGG
CAGTGGAATGCGTGCCGTACACTCAGGCCCTTACGCCAGCCACTGCCCTAGACCTGGTCC
GCCGATATGATGTGGTGGCTGACTGCTCGGACAACGTGCCCACTCGCTACCTGGTTAATG
ACGCATGTGTGCTGGCGGGTCGGCCCCTCGTGTCTGCCAGTGCCTTGCGCTTCGAGGGCC
AAATCACAGTCTACCATTATGACGGTGGCCCTTGCTATCGCTGCATATTCCCCCAACCAC
CCCCAGCGGAGACAGTGACCAACTGCGCGGACGGCGGGGTGCTCGGTGTCGTTACCGGGG
TCCTGGGCTGCCTGCAGGCCTTGGAAGTGCTGAAAATCGCTGCGGGTCTGGGCCCCTCTT
ACAGTGGCAGCTTGTTGCTCTTTGATGCCCTGAGAGGGCATTTCCGCTCTATTCGGCTGC
GGAGCCGCAGGCTCGACTGTGCAGCTTGCGGGGAACGGCCCACTGTGACTGATCTGCTGG
ACTATGAAGCCTTCTGTGGCTCCTCAGCCACTGATAAATGCCGCTCCCTGCAACTACTGA
GCCCAGAGGAGCGTGTTTCTGTCACCGACTATAAGCGACTGCTGGATTCTGGGGCATTCC
ACCTGTTGCTGGACGTCAGGCCTCAGGTGGAGGTGGACATTTGTCGTTTGCCTCATGCCC
TACACATCCCTCTGAAACATTTGGAACGCAGGGATGCGGAGAGCCTGAAACTCTTAAAAG
AAGCAATCTGGGAAGAGAAGCAGGGCACACAAGAAGGGGCTGCTGTCCCCATTTATGTGA
TTTGCAAACTGGGAAATGACTCACAGAAAGCCGTGAAGATCCTCCAGTCCTTATCAGCAG
CTCAAGAGTTAGACCCTTTAACAGTTCGGGATGTTGTGGGGGGCCTCATGGCCTGGGCTG
CCAAAATCGATGGAACATTTCCACAGTACTGAGGTGACTGGTATAGTCTGATGAGAAAGA
TGTGGATTGCCATAATACCTCAAAGATACACTTGTTTGCATTTTTCGGTAATATACATAG
GAGCTGGGGATTCTACAGTATCTGTGAATACGTGGACTCCTTTTTATAAGGAGTTTTAAA
AATTGTTATGTATTGGATGAATGACTTATTAATGGATTATACCGTTTCTGAGAACCATCA
TTTTTTTTTTCAGCACACGGAGGATGTCTTGGACATGTGAGATGTAACGTGACAGGATTT
TGCATTTTAAACTGCAGATCATTTACATGTCCTTATTTTCCACCTCCCCCAGTCAAAATG
CTTTCTAAATCATTTTCACAGATTATATACTTCGGATCTGTTTACTGTTCAGTTAAGAAA
TTCTTGGATCTTATTAATATTTCAGATGATAGAATACATCCTACAGAAATACATGTTTAA
AATGTAAATTGTTTTAATTATTCAGAAAGAGGGTCATTCTATTTGGCCTTTTGAATTAGT
ATCAAAATGAGATTTTTTTTTTTTTGAGATGGAATCTCTCTCTGTTGCCCAGGCTGGAGT
GTAGTGGTGCGATCTCAGCTCACTGCAACCTCCGCCTCCCAGGTTCAAGCGATTCTCCTG
CCTCAGCCTCCTGAGTAGCTGGGATTACAGGCGCACGCCACCACGCCTGGCTAATTTTTG
TATTTTTAGTACAGAGGGGGTTTCACCATGTTTGTCAGGCTGGTCTCGAACTCCTGACCT
CGTGATCCGCCTGCCTCGACTTCCCAGAGTGCTGGGATTACAGGCGTGAGCCACCACACC
CTCCCGAGATCTTTAAAGTTTACCAAAATAAAGGAAACCACCTTTGTAGTCTGAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA

Protein sequence predicted based on nucleotide sequence

MASREEVLALQAEVAQREEELNSLKQKLASALLAEQEPQPERLVPVSPLPPKAALSRDEI
LRYSRQLVLPELGVHGQLRLGTACVLIVGCGGLGCPLAQYLAAAGVGRLGLVDYDVVEMS
NLARQVLHGEALAGQAKAFSAAASLRRLNSAVECVPYTQALTPATALDLVRRYDVVADCS
DNVPTRYLVNDACVLAGRPLVSASALRFEGQITVYHYDGGPCYRCIFPQPPPAETVTNCA
DGGVLGVVTGVLGCLQALEVLKIAAGLGPSYSGSLLLFDALRGHFRSIRLRSRRLDCAAC
GERPTVTDLLDYEAFCGSSATDKCRSLQLLSPEERVSVTDYKRLLDSGAFHLLLDVRPQV
EVDICRLPHALHIPLKHLERRDAESLKLLKEAIWEEKQGTQEGAAVPIYVICKLGNDSQK
AVKILQSLSAAQELDPLTVRDVVGGLMAWAAKIDGTFPQY

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pANT7_cGST

Synonyms:

pANT7-cGST

Sequencing Primer:

Forward: NAP150

Reverse: NAP138R

Description:

with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial

Comments:

One in a series of pANT7 vectors made by A. Lau and N. Ramachandran for use with NAPPA arrays. Can be used for cell free expression of the gene insert using human IVTT from Thermo Fisher or rabbit reticulocyte systems

Size (bp):

5964

Parent Vector:

None

Properties:

Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker

Author Name:

Joshua LaBaer
Al Lau
Niroshan Ramachandran

Publications:

PMID: 15232106

Title: Self-assembling protein microarrays

Vector Map:

Vector Sequence:

Sequence:

GGGCGAATTAATTCCGGTTATTTTCCACCATATTGCCGTCTTTTGGCAATGTGAGGGCCCGGAAACCTGGCCCTGTCTTCTTGACGAGCATTCCTAGGGGTCTTTCCCCTCTCGCCAAAGGAATGCAAGGTCTGTTGAATGTCGTGAAGGAAGCAGTTCCTCTGGAAGCTTCTTGAAGACAAACAACGTCTGTAGCGACCCTTTGCAGGCAGCGGAACCCCCCACCTGGCGACAGGTGCCTCTGCGGCCAAAAGCCACGTGTATAAGATACACCTGCAAAGGCGGCACAACCCCAGTGCCACGTTGTGAGTTGGATAGTTGTGGAAAGAGTCAAATGGCTCACCTCAAGCGTATTCAACAAGGGGCTGAAGGATGCCCAGAAGGTACCCCATTGTATGGGATCTGATCTGGGGCCTCGGTGCACATGCTTTACATGTGTTTAGTCGAGGTTAAAAAACGTCTAGGCCCCCCGAACCACGGGGACGTGGTTTTCCTTTGAAAAACACGATGATAATATGGATCGGATCCGAATTCGAGCTCCGTCATCAACAAGTTTGTACAAAAAAGCTGAACGAGAAACGTAAAATGATATAAATATCAATATATTAAATTAGATTTTGCATAAAAAACAGACTACATAATACTGTAAAACACAACATATCCAGTCACTATGGCGGCCGCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATAATGTGTGGATTTTGAGTTAGGATCCGTCGAGATTTTCAGGAGCTAAGGAAGCTAAAATGGAGAAAAAAATCACTGGATATACCACCGTTGATATATCCCAATGGCATCGTAAAGAACATTTTGAGGCATTTCAGTCAGTTGCTCAATGTACCTATAACCAGACCGTTCAGCTGGATATTACGGCCTTTTTAAAGACCGTAAAGAAAAATAAGCACAAGTTTTATCCGGCCTTTATTCACATTCTTGCCCGCCTGATGAATGCTCATCCGGAATTCCGTATGGCAATGAAAGACGGTGAGCTGGTGATATGGGATAGTGTTCACCCTTGTTACACCGTTTTCCATGAGCAAACTGAAACGTTTTCATCGCTCTGGAGTGAATACCACGACGATTTCCGGCAGTTTCTACACATATATTCGCAAGATGTGGCGTGTTACGGTGAAAACCTGGCCTATTTCCCTAAAGGGTTTATTGAGAATATGTTTTTCGTCTCAGCCAATCCCTGGGTGAGTTTCACCAGTTTTGATTTAAACGTGGCCAATATGGACAACTTCTTCGCCCCCGTTTTCACCATGGGCAAATATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGGCGATTCAGGTTCATCATGCCGTCTGTGATGGCTTCCATGTCGGCAGAATGCTTAATGAATTACAACAGTACTGCGATGAGTGGCAGGGCGGGGCGTAAAGATCTGGATCCGGCTTACTAAAAGCCAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGAATATATACTGATATGTATACCCGAAGTATGTCAAAAAGAGGTGTGCTATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCAATATCTCCGGTCTGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGAAATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACACGCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGGTGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTGGCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAAATGTCAGGCTCCCTTATACACAGCCAGTCTGCAGGTCGACCATAGTGACTGGATATGTTGTGTTTTACAGTATTATGTAGTCTGTTTTTTATGCAAAATCTAATTTAATATATTGATATTTATATCATTTTACGTTTCTCGTTCAGCTTTCTTGTACAAAGTGGTTGATGACAAGCTTGCGGCCGCACTCGAGCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCACTCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATGAGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTGGAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACAGTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTGGTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTGGATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAACTCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCGAAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCATCCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCCAATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTGAAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCATGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCCAAAATCGGATCTGGTTCCGCGTTGAAGATCTGACTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTTTAAACACTAGTCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAAGGAGGAACTATATCCGGGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGAAATTGTAAACGTTAATATTTTGTTAAAATTCGCGTTAAATTTTTGTTAAATCAGCTCATTTTTTAACCAATAGGCCGAAATCGGCAAAATCCCTTATAAATCAAAAGAATAGACCGAGATAGGGTTGAGTGTTGTTCCAGTTTGGAACAAGAGTCCACTATTAAAGAACGTGGACTCCAACGTCAAAGGGCGAAAAACCGTCTATCAGGGCGATGGCCCACTACGTGAACCATCACCCTAATCAAGTTTTTTGGGGTCGAGGTGCCGTAAAGCACTAAATCGGAACCCTAAAGGGAGCCCCCGATTTAGAGCTTGACGGGGAAAGCCGGCGAACGTGGCGAGAAAGGAAGGGAAGAAAGCGAAAGGAGCGGGCGCTAGGGCGCTGGCAAGTGTAGCGGTCACGCTGCGCGTAACCACCACACCCGCCGCGCTTAATGCGCCGCTACAGGGCGCGTCGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATTGTAATACGACTCACTATA

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ColE

ColE1 bacterial origin of replication

3931

3249

death cassette

ccdB

ccdB death cassette present in 'empty vector' form only (replace with gene of interest)

1876

2085

primer

NAP138

NAP138 reverse sequencing primer 5? TGTTTCGCCATTTATCACCTTC 3?

2405

2384

primer

NAP150

Forward sequencing primer NAP150 5?-CCC ATT GTA TGG GAT CTG ATC-3?

388

408

promoter

T7 transcriptional start sequence

5940

5964

recombination site

attR1

AttR1 site for recombination in empty vector

549

565

recombination site

attR2

AttR2 site for recombination in empty vector

2236

2251

selectable marker

CmR

chloramphenicol resistance

782

1438

selectable marker

AmpR

ampicillin resistance gene

4688

4029

tag

GST

Glutathione transferase (GST) tag

2282

2950

Gene

Gene Symbol :

UTY

Symbol Nomenclature :

UTY

SYNONYM :

KDM6AL; UTY1

Designation :

histone demethylase UTY|ubiquitous TPR motif protein UTY|ubiquitously transcribed TPR gene on Y chromosome|ubiquitously transcribed tetratricopeptide repeat gene, Y chromosome|ubiquitously transcribed tetratricopeptide repeat gene, Y-linked|ubiquitously-transcribed TPR protein on the Y chromosome|ubiquitously-transcribed Y chromosome tetratricopeptide repeat protein

Full Nomenclature :

ubiquitously transcribed tetratricopeptide repeat containing, Y-linked

GENEID :

7404

HGNC :

12638

MIM :

400009

Vega :

OTTHUMG00000036319

Target GenBank:

BC015939

Reference Sequence Alignment

  

HsCD00603863      -------CGGACGCGTGGGTTCCGGAAGAGGTCGCCATGGCTTCCCGGGAGGAGGTACTC
NM_182660.1       ATGAAATCCTGCGCAGTGTCGCTCACTACCGCCGCTGTTGCCTTCGGTGATGAGGCAAAG
                         *  .***.  *   *  ..:.. * *** .* ** * * * ** **** *.: 

HsCD00603863      GCCTTACAAGCTGAAGTTGCCCAACGTGAGGAGGAATTGAATTCGCTGAAGCAGAAGCTG
NM_182660.1       AAAATGGCGGAAGGAAAAGCGAGCCGCGAGAGTGAAGAGGAGTCTGTTAGCCTGACAGTC
                  ...:*. ..*.:*.*.::** ...** ***.. *** :*.* **  * *. *:**.. * 

HsCD00603863      GCGTCGG--------CTCTTTTGGCTGAGCAGGAACCGCAGCCAGAACGGCTGGTTCCGG
NM_182660.1       GAGGAAAGGGAGGCGCTTGGTGGCATGGACAGCCGTCTCTTCGGGTTCGTGAGGCTTCAT
                  *.* ...        **   * * .**..*** .. * *: * .*::**  :** * *. 

HsCD00603863      TGTCGCCGCTGCCGCCGAAGGCCGCTCTGTCCCGAGATGAGATTCTGCGCTATAGCCGGC
NM_182660.1       GAAGATGGCGCCAGAACGAAGACCCTACTAGGCAAGGCTGTTCGCTGCTACGAATCTTTA
                   .: .  **  *.*.. .*.*.* **.  :  *.**.  . :  **** . .:* *   .

HsCD00603863      AGCTAGTGCTGCCCGAGCTGGGCGTGCACGGACAGCTGCG--------------------
NM_182660.1       ATCTTAAAAGCTGAAGGAAAAGTGGAGTCTGACTTCTTTTGCCAATTAGGTCACTTCAAC
                  * **:.:..    ...*.:..* * . :* ***: **                       

HsCD00603863      -------------------------------------------------CCTGGGGACCG
NM_182660.1       CTCTTGTTGGAAGATTATTCAAAAGCATTATCTGCATATCAGAGATATTACAGTTTACAG
                                                                   .*:*   **.*

HsCD00603863      CGTGCGTGCTAATCGTGGGCTGCGGTGGACTCGG--------------------------
NM_182660.1       GCTGACTACTGGAAGAATGCTGCGTTTTTATATGGCCTTGGTTTGGTCTACTTCTACTAC
                    **. *.**..:.*:. ****** *  :.*. *                          

HsCD00603863      --------------------------------------CTGTCCACTAGCGCAGTACTTG
NM_182660.1       AATGCATTTCATTGGGCAATTAAAGCATTTCAAGATGTCCTTTATGTTGACCCCAGCTTT
                                                        *  * .: *:*. *. :.*** 

HsCD00603863      GCAGCGGCCGGCGTGGGCCGCCTTGGCCTTGTGGACTATGACGTGGTAGAGATGAGCAAC
NM_182660.1       TGTCGAGCCAAGGAAATTCATTTACGACTTGGGCTCATGTTCAAAGTGAACACAGACTAC
                    :  .***.. *:..  *.  *: *.**** * :*::  :*.:.**..* * ...*:**

HsCD00603863      CTGGCCCGCCAAGTGCTGCATGGCGAGGCACTGGCTGGCCAGGCCAAGGCCTTTTCGGCC
NM_182660.1       AAGTCTAGTTTAAAGCATTTTCAGTTAGCCTTGATTGACTGTAATCCATGTACTTTGTCC
                  .:* * .*  :*.:**:  :* .  :.**. **. **.* . .. ...   : ** * **

HsCD00603863      GCCGCCTCGCTGCGCCGCCTCAATTCGGCAGTGGAATGCGTGCCGT--------------
NM_182660.1       AATGCTGAAATTCAATTTCATATTGCCCATTTGTATGAAACCCAGAGGAAGTATCATTCT
                  .. **  ...* *..   *: *:* *  .: ** *: ...  *.*:              

HsCD00603863      -------------------------------------ACACTCAGGCCCTTACGCCAGCC
NM_182660.1       GCAAAGGAGGCATATGAACAACTTTTGCAGACAGAAAACCTTCCTGCACAAGTAAAAGCA
                                                       **. **. **.*::. ...***.

HsCD00603863      ACTGCCCTAGACCTGGTCCGCCGATATGATGTGGTGGCTGACTGCTCGGACAACGTGCCC
NM_182660.1       ACTGTATTGCAACAGTTAGGTTGGATGCATCATAATATGGATCTAGTAGGAGACAAAGCC
                  **** . *. *.*:* *. *  *.::  ** : .: .  **   .  .*...**.:. **

HsCD00603863      ACTCG-------CTACCTGGTTAATGACGCATGTGTGCTGGCGGGTCGGCCCCTCGTGTC
NM_182660.1       ACAAAGGAAAGCTATGCTATTCAGTATCTCCAAAAGTCTTTGGAGGCAGATCCTAATTCT
                  **:..        :: **. * *.*.:* *.:.:.  **   *.* *.*. ***..*   

HsCD00603863      TGCCAGTG-------------------------------------------CCTTGCGCT
NM_182660.1       GGCCAATCGTGGTATTTTCTTGGAAGGTGTTATTCAAGTATTGGGAAAGTTCAGGATGCC
                   ****.*                                            *.  . ** 

HsCD00603863      TCGAGGGCCAAATCACAGTCTACCATTATGACGGTGGCCCTTGCTATCGCTGCATATTCC
NM_182660.1       TTTATATCTTACAGGCAATCTATTGATAAATCAGAAGCAAGTGCAGATACATGGTGTTCA
                  *  * . * :*.: .**.****  .:**:.:*.*:.**.. ***:.: .*:  .*.***.

HsCD00603863      CCCAACCACCCCCAGCGGAGACAGTGACCAACTGCGCGG-----ACGGCGGGGTGCTCGG
NM_182660.1       ATAGGTGTGTTGTATCAGCAGCAAAATCAGCCTATGGATGCTTTACAGGCATATATTTGT
                  . ...  :     * *.*...**.:.:*...**. * .      **.*  . .*. * * 

HsCD00603863      TGTCGTTACCGGGGTCCTGGGCTGCCTGCAGGCCTTGGAAGTGCTGAAAATCGCTGCGGG
NM_182660.1       GCTGTACAATTGGACCATGGGCATGCCGCAGCCTGGATGGACCTAGGTACTCTCTATGAA
                    *  : *.  **. *.*****:  * **** *   . ...   :*.:*.** **. *..

HsCD00603863      TCTGGGCCCCTCTTACAG------------TGGCAGCTTGTTGCTCTTTGATGCCCTGAG
NM_182660.1       TCCTGCAATCAACCTCAAGATGCCATTAAATGCTACCTAAATGCAGCTAGAAGCAAACGT
                  **  * .. *:.  :**.            **  * **:.:***:  *:**:**..: . 

HsCD00603863      AGGGCATTTCCGCTCTATTCGGCTGCGGAGCCGCAGGCTCGACTGTGCAGCTTG------
NM_182660.1       TGTAGTAATACCTCTACGCTTGCTGCAAGAATTAAATTTCTACAGAATGGTTCTGATAAC
                  :* . :::*.*    :.    *****.....  .*.  ** **:*:. .* *        

HsCD00603863      --CGGGGAACGGCCCACTGTGACTGATCTGCTGGACTATGAAGCCTTCTGTGGCTCCTCA
NM_182660.1       TGGAATGGTGGCCAGAGTCTTTCACATCATCCAGTACAGCAAGTTTATTCGTTGTGTTTG
                     .. *.: * *. * * * :*: ***: * .*:. *  ***  *: *     *  * .

HsCD00603863      GCCACTGATAAATGCCGCTCCCTGCAACTACTGAGCCCAGAGGAGCGTGTTTCTGTCACC
NM_182660.1       ACACCACAGAAATTACAGCACTTGGAACAACTGCGAGCAAATAGAGATAATTTAAATCCA
                  .*..*: * **** .*.  .* ** ***:****.*. **.* ... .*.:** :.: .*.

HsCD00603863      GACTATAAGCGACTGCTGGATTCTGGGGCATTCCACCTG---------------------
NM_182660.1       GCACAGAAGCATCAGCTGGAACAGTTAGAAAGTCAGTTTGTCTTAATGCAGCAAATGAGA
                  *.. * ****.:*:******: .   .*.*:  **  *                      

HsCD00603863      ------------------------------------------------------------
NM_182660.1       CACAAAGAAGTTGCTCAGGTACGAACTACTGGAATTCATAACGGGGCCATAACTGATTCA
                                                                              

HsCD00603863      -------------------TTGCTGGACGTCAGGCCTCAGGTGGAGGTGGACATTTGTCG
NM_182660.1       TCACTGCCTACAAACTCTGTCTCTAATCGACAACCACATGGTGCTCTGACCAGAGTATCT
                                     *  **..:**:**. *. .:**** :   . ...: *.** 

HsCD00603863      TTTGCCTCATGCCCTACACATCCCTCTGAAACATTTGGAACGCAGGGATGCGGAGAGCCT
NM_182660.1       AGCGTCTCTCAGCCTGGAGTTCGCCCTGCTTGTGTTGAAAAACTTTTGTCCAGTGGAGCT
                  :  * ***: . ***. * :** * ***.:: : ***.**..*:   .* *.*:*.. **

HsCD00603863      GAAACTCTTAAAAGAAGCAATCTGGGAAGAGAAGCAGGGCACACAAGAAGGGGCTGCTGT
NM_182660.1       TTTTCTGCAGGCTGTATTCCTTGTGGCACATCAAAAATTCTAGGAAGTACAGACACTATC
                   :::**  :...:*:*  ..*   **.* * .*..*.  *:.. ***:* .*.*:  :  

HsCD00603863      CCCCATTTATGTGATTTGCAAACTGGGAAATGACTCACAGAAAGCCG-------------
NM_182660.1       TTGCTAGGCAGTAATTGTATAGCAGGAAGTGAAAGTAATGGAAATGTGCCTTACCTGCAG
                     *::  .:**.***  .:*.*:**.*.: .*.  *.:*.**.                

HsCD00603863      --------------------TGAAGATCCTCCAGTCCTTATCAGCAGCTCAAGAGT----
NM_182660.1       CAAAATACACACACTCTACCTCATAATCATACAGACCTGAACAGCAGCACAGAAGAGCCA
                                      * *:.***.*.***:*** *:*******:**..**:    

HsCD00603863      ----------TAGACCCTTTAACAGTTCGGGATGTTGTGGGGGGCCTCATGGCCTGGGCT
NM_182660.1       TGGAGAAAACAGCTATCTAACTCCGCTCAGGGGCTTCATAAAAGTCAGAGTTCATGTTTG
                            :. :. **::.:*.* **.**.  ** : ....* *: *   *.**    

HsCD00603863      GCCAAAATCGATGGAACATTTCCACAGTACTGAGGTG------ACTGGTATAGTCTGATG
NM_182660.1       TCAGGACCTAATGAAGAACAACCTCTGTTTTCCACTGGGTCAGCCCAGTATCACCAGGCA
                   *...*.  .***.*..* ::**:*:**: * .. **      .* .****.. *:*. .

HsCD00603863      AGAAAGATGTGGATTGCCATAATACCTCAAAGATACACTTGTTTGCATTTTTCG------
NM_182660.1       ACTAGCACTGGTATTAAGAAGGCGAATGAACATCTCACTCTGCCTAGTAATTCAGTACCA
                  * :*. *   * ***.. *:.. ...* **..: :****      ..*::***.      

HsCD00603863      ------------------------------------------------------------
NM_182660.1       CAGGGGGATGCTGACAGTCACCTCTCCTGTCATACTGCTACCTCAGGTGGACAACAAGGC
                                                                              

HsCD00603863      -------------------------GTAATATACATAGGAGCTGGGGATTCTACAGTATC
NM_182660.1       ATTATGTTTACCAAAGAGAGCAAGCCTTCAAAAAATAGATCCTTGGTGCCTGAAACAAGC
                                            *:.:*:*.****.: ** ** .    *.* :* *

HsCD00603863      TGTGAATACGTGGACTCCTTTTTATAAGGAGTTTTAAAAATTGTTATGTATTGGATGAAT
NM_182660.1       AGGCATACTGGAGACACATCTAATGGCTGTGCTGATGTCAAGGGACTTTCTAATCATGTT
                  :*  *::. * .***:*.* *::: .. *:* * ::.:.*: * :.* *.*:. .: .:*

HsCD00603863      GACTTATTAATGGATTATACCGTTTCTG--------------------------------
NM_182660.1       CATCAGTTGATAGCAGATGCTGTTTCCAGTCCTAACCATGGAGATTCACCAAATTTATTA
                   *  :.**.**.*.: **.* ***** .                                

HsCD00603863      -----------------AGAACCATCATTTTTTTTTTCAGCACACGGAGGATGTCTTGGA
NM_182660.1       ATTGCAGACAATCCTCAGCTCTCTGCTTTGTTGATTGGAAAAGCCAATGGCAATGTGGGT
                                   . :. *: *:** ** :**  *..* .*..:**.:.* * **:

HsCD00603863      CATGTGAGATGTAACGTGACAGGATTTTGCATTTTAAACTGCAGATCATTTACATGTCCT
NM_182660.1       ACTGGAACCTGTGACAAAGTGAATAATATTCACCCAGCTGTTCATACAAAGACTGATCAT
                  ..** .* .***.**.:.. ...:::*:  .:   *..    ..::**:: **: .**.*

HsCD00603863      TATTTTCCACCTCCCCCAGTCAAAATGCTTTCTAAATCATTTTCACAGATTATATACTTC
NM_182660.1       TCTGTTGCCTCTTCACCCTCTTCAGCCATTTCCACAGCAACACCTTCTCCTAAATCCACT
                  *.* ** *. ** *.**.   :.*.  .**** *.* **: : *: . . **:**.*:  

HsCD00603863      GG----------------------------------------------------------
NM_182660.1       GAGCAGAGAAGCATAAACAGTGTTACCAGCCTTAACAGTCCTCACAGTGGATTACACACA
                  *.                                                          

HsCD00603863      ------------------------------------------------------------
NM_182660.1       GTCAATGGAGAGGGGCTGGGGAAGTCACAGAGCTCTACAAAAGTAGACCTGCCTTTAGCT
                                                                              

HsCD00603863      ------------------------------------------------------------
NM_182660.1       AGCCACAGATCTACTTCTCAGATCTTACCATCAATGTCAGTGTCTATATGCCCCAGTTCA
                                                                              

HsCD00603863      --------------ATCTGTTTACTGTTCAGTTAAGAAATTCTTGGATCTTATTAATATT
NM_182660.1       ACAGAAGTTCTGAAAGCATGCAGGAATCCAGGTAAAAATGGCTTGTCTAATAGCTGCATT
                                * *:   :. :.* *** ***.**:  **** .*.:**  :. ***

HsCD00603863      TCAGATGATAGAATACATCCTACAG-----------------------------------
NM_182660.1       TTGTTAGATAAATGTCCACCTCCAAGACCACCAACTTCACCATACCCACCCTTGCCAAAG
                  * . ::****.*: :*.:***.**.                                   

HsCD00603863      ---------------------------AAATACATGTTTAAAATGTAAATTGTTTTAATT
NM_182660.1       GACAAGTTGAATCCACCCACACCTAGTATTTACTTGGAAAATAAACGTGATGCTTTCTTT
                                             *::***:** ::**:*:. .:.:** ***.:**

HsCD00603863      ATTCAGAAAGAGGGTCATTCTATTTGGCCTTTTGAATTAGTATCAAAATGAG--------
NM_182660.1       CCTCCATTACATCAATTTTGTACAAATCCAAAAAACCCTGTTACAGTAATACGTGGCCTT
                  . **..::* *  .: :** ** ::. **::::.*.  :**::**.:*: *         

HsCD00603863      -------------------ATTTTTTTTTTTTTGAGATGGAATCTCTCTCTGTTGCCCAG
NM_182660.1       GCTGGAGCTCTTAAATTAGATCTTGGACTTTTCTCTACCAAAACTTTGGTAGAAGCTAAC
                                     ** **  : ****  . *  .**:** *   :*::** .* 

HsCD00603863      GCTGGAGTGTAGTGGTGCGATCTCAGCTCACTGCAACCTCCGCCTCCCAGGTTCAAGCGA
NM_182660.1       AATGAACATATGGTAGAAGTGAGGACACAGTTGCTGCAACCAGCAGATGAAAACTGGGAT
                  ..**.* : ::*  . ..*: .  * . .. ***:.*.:**. *: . ...::*:.* .:

HsCD00603863      TTCTCCTGCCTCAG--------------------------------CCTCCTGAGTAGCT
NM_182660.1       CCCACTGGAACAAAGAAAATCTGGCGTTGTGAAAGCAATAGATCTCATACTACAATTGCC
                    *:*  *.. .*.                                . :* : *.*:** 

HsCD00603863      GGGATTACAGGCGCACGCCACCACGCCTGGCTAATTTTTGTATTTTTAGTACAGAGGGGG
NM_182660.1       AAATACGCACAATACCAGGCTTCCTCCTTCCAGGAATCATTGAGAGCTGGAATGCAATGG
                  ...:: .** .. ..*.  .  .* ***  *:..::* : *.: :  :* *.:*... **

HsCD00603863      TTTCACCATGTTTGTCAGGCTGGTCTCGAACTCCTGACCTCGTGATCCGCCTGCCTCGAC
NM_182660.1       TGTGATCTCAGCTCACTGCAGCCTCCGCCTCCTGGGTTCAAGCGATTCTCCCACCTCAGC
                  * * * *: .  * :*:* .   **   .:*    *: *:.* *** * ** .****..*

HsCD00603863      TTCCCAGAGTGCTGGGATTACAGGCGTGAGCCACCACACCCTCCCGAGATCTTTAAAGTT
NM_182660.1       CTCCCGAATAGCTGGAATTACAGGCACCTGCCATCATGCCCAACTAATTTTTGTATTTTT
                   ****..* :*****.*********.  :**** ** .***:.* .* :* * **:: **

HsCD00603863      TACCAAAATAAAGGAAACCACCTTTGTAGTCTGAAAAAAAAAAAAAAAAAAAAAAAAAAA
NM_182660.1       GTAGAGACAGGGTTTCACCATGTTGGCCAGGCTTGTCTTGAACTCCTGACCTCAGGTGGT
                   :. *.*.:...  :.****  ** * ..    :.:.::.**.:..:.*..:.*..:..:

HsCD00603863      AAAAAAAAA---------------------------------------------------
NM_182660.1       CTGCTTGCCTCAGCATCCCAAAGTGCTGGGATTACAGGTGTGAGCCACCATGCCCGGTAA
                  .:..::...

Pathways

Reactome :

Defective AMN causes hereditary megaloblastic anemia 1

Reactome :

Defective BTD causes biotidinase deficiency

Reactome :

Defective CD320 causes methylmalonic aciduria

Reactome :

Defective CUBN causes hereditary megaloblastic anemia 1

Reactome :

Defective GIF causes intrinsic factor deficiency

Reactome :

Defective HLCS causes multiple carboxylase deficiency

Reactome :

Defective LMBRD1 causes methylmalonic aciduria and homocystinuria type cblF

Reactome :

Defective MMAA causes methylmalonic aciduria type cblA

Reactome :

Defective MMAB causes methylmalonic aciduria type cblB

Reactome :

Defective MMACHC causes methylmalonic aciduria and homocystinuria type cblC

Reactome :

Defective MMADHC causes methylmalonic aciduria and homocystinuria type cblD

Reactome :

Defective MTR causes methylmalonic aciduria and homocystinuria type cblG

Reactome :

Defective MTRR causes methylmalonic aciduria and homocystinuria type cblE

Reactome :

Defective MUT causes methylmalonic aciduria mut type

Reactome :

Defective TCN2 causes hereditary megaloblastic anemia

Reactome :

Defects in biotin (Btn) metabolism

Reactome :

Defects in cobalamin (B12) metabolism

Reactome :

Defects in vitamin and cofactor metabolism

Reactome :

Disease

Reactome :

Metabolism

Reactome :

Metabolism of vitamins and cofactors

Reactome :

Metabolism of water-soluble vitamins and cofactors

Reactome :

Molybdenum cofactor biosynthesis

Protein

SMART domain :

RHOD : Rhodanese Homology Domain

UniProt :

O95396

HPRD :

02451

Clone

Cloning Information :

644094

HIP Master Clone ID :

160426

IMAGE ID :

3908290

Original Clone ID :

FLH225107.01L

Original Clone ID :

HsCD00078426

Organism

Species Specific ID:

27304

Genome

Chromosome :

Map Location :

Yq11

Ensembl :

ENSG00000183878

Reagents

Labome :

MOCS3-antibody

MOCS3 (H. sapiens )In pANT7_cGST (GST-taggedIn vitro expression vector)

Coding Sequence Details

Recommended Growth Condition:

Vector Features:

Gene

Reference Sequence Alignment

Pathways

Protein

Clone

Organism

Genome

Reagents

MOCS3 (H. sapiens )
In pANT7_cGST (GST-tagged
In vitro expression vector)