Gene:
Gene Symbol:
EPHA8
Gene Name:
EPH receptor A8
Original Clone ID:
HsCD00302778
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pANT7_cGST
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
Identical to HsCD00731646
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00604121
Price:
Login for Pricing
UNAVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 3015nts
Open reading frame: 126 to 3140
GCGCCGGGGTGTGCGCCCGGCCGGGTGTGCGGAGAGCGAGGGAGCGCGCTCCCTCCCGAC
GCGCGGGCCGCAGCGGCCAAGCCCGAGGGTGCGTGGCGCCCCCGCCCGCCCGGCCCGGCC
CGGCCATGGCCCCCGCCCGGGGCCGCCTGCCCCCTGCGCTCTGGGTCGTCACGGCCGCGG
CGGCGGCGGCCACCTGCGTGTCCGCGGCGCGCGGCGAAGTGAATTTGCTGGACACGTCGA
CCATCCACGGGGACTGGGGCTGGCTCACGTATCCGGCTCATGGGTGGGACTCCATCAACG
AGGTGGACGAGTCCTTCCAGCCCATCCACACGTACCAGGTTTGCAACGTCATGAGCCCCA
ACCAGAACAACTGGCTGCGCACGAGCTGGGTCCCCCGAGACGGCGCCCGGCGCGTCTATG
CTGAGATCAAGTTTACCCTGCGCGACTGCAACAGCATGCCTGGTGTGCTGGGCACCTGCA
AGGAGACCTTCAACCTCTACTACCTGGAGTCGGACCGCGACCTGGGGGCCAGCACACAAG
AAAGCCAGTTCCTCAAAATCGACACCATTGCGGCCGACGAGAGCTTCACAGGTGCCGACC
TTGGTGTGCGGCGTCTCAAGCTCAACACGGAGGTGCGCAGTGTGGGTCCCCTCAGCAAGC
GCGGCTTCTACCTGGCCTTCCAGGACATAGGTGCCTGCCTGGCCATCCTCTCTCTCCGCA
TCTACTATAAGAAGTGCCCTGCCATGGTGCGCAATCTGGCTGCCTTCTCGGAGGCAGTGA
CGGGGGCCGACTCGTCCTCACTGGTGGAGGTGAGGGGCCAGTGCGTGCGGCACTCAGAGG
AGCGGGACACACCCAAGATGTACTGCAGCGCGGAGGGCGAGTGGCTCGTGCCCATCGGCA
AATGCGTGTGCAGTGCCGGCTACGAGGAGCGGCGGGATGCCTGTGTGGCCTGTGAGCTGG
GCTTCTACAAGTCAGCCCCTGGGGACCAGCTGTGTGCCCGCTGCCCTCCCCACAGCCACT
CCGCAGCTCCAGCCGCCCAAGCCTGCCACTGTGACCTCAGCTACTACCGTGCAGCCCTGG
ACCCGCCGTCCTCAGCCTGCACCCGGCCACCCTCGGCACCAGTGAACCTGATCTCCAGTG
TGAATGGGACATCAGTGACTCTGGAGTGGGCCCCTCCCCTGGACCCAGGTGGCCGCAGTG
ACATCACCTACAATGCCGTGTGCCGCCGCTGCCCCTGGGCACTGAGCCGCTGCGAGGCAT
GTGGGAGCGGCACCCGCTTTGTGCCCCAGCAGACAAGCCTGGTGCAGGCCAGCCTGCTGG
TGGCCAACCTGCTGGCCCACATGAACTACTCCTTCTGGATCGAGGCCGTCAATGGCGTGT
CCGACCTGAGCCCCGAGCCCCGCCGGGCCGCTGTGGTCAACATCACCACGAACCAGGCAG
CCCCGTCCCAGGTGGTGGTGATCCGTCAAGAGCGGGCGGGGCAGACCAGCGTCTCGCTGC
TGTGGCAGGAGCCCGAGCAGCCGAACGGCATCATCCTGGAGTATGAGATCAAGTACTACG
AGAAGGACAAGGAGATGCAGAGCTACTCCACCCTCAAGGCCGTCACCACCAGAGCCACCG
TCTCCGGCCTCAAGCCGGGCACCCGCTACGTGTTCCAGGTCCGAGCCCGCACCTCAGCAG
GCTGTGGCCGCTTCAGCCAGGCCATGGAGGTGGAGACCGGGAAACCCCGGCCCCGCTATG
ACACCAGGACCATTGTCTGGATCTGCCTGACGCTCATCACGGGCCTGGTGGTGCTTCTGC
TCCTGCTCATCTGCAAGAAGAGGCACTGTGGCTACAGCAAGGCCTTCCAGGACTCGGACG
AGGAGAAGATGCACTATCAGAATGGACAGGCACCCCCACCTGTCTTCCTGCCTCTGCATC
ACCCCCCGGGAAAGCTCCCAGAGCCCCAGTTCTATGCGGAACCCCACACCTACGAGGAGC
CAGGCCGGGCGGGCCGCAGTTTCACTCGGGAGATCGAGGCCTCTAGGATCCACATCGAGA
AAATCATCGGCTCTGGAGACTCCGGGGAAGTCTGCTACGGGAGGCTGCGGGTGCCAGGGC
AGCGGGATGTGCCCGTGGCCATCAAGGCCCTCAAAGCCGGCTACACGGAGAGACAGAGGC
GGGACTTCCTGAGCGAGGCGTCCATCATGGGGCAATTCGACCATCCCAACATCATCCGCC
TCGAGGGTGTCGTCACCCGTGGCCGCCTGGCAATGATTGTGACTGAGTACATGGAGAACG
GCTCTCTGGACACCTTCCTGAGGACCCACGACGGGCAGTTCACCATCATGCAGCTGGTGG
GCATGCTGAGAGGAGTGGGTGCCGGCATGCGCTACCTCTCAGACCTGGGCTATGTCCACC
GAGACCTGGCCGCCCGCAACGTCCTGGTTGACAGCAACCTGGTCTGCAAGGTGTCTGACT
TCGGGCTCTCACGGGTGCTGGAGGACGACCCGGATGCTGCCTACACCACCACGGGCGGGA
AGATCCCCATCCGCTGGACGGCCCCAGAGGCCATCGCCTTCCGCACCTTCTCCTCGGCCA
GCGACGTGTGGAGCTTCGGCGTGGTCATGTGGGAGGTGCTGGCCTATGGGGAGCGGCCCT
ACTGGAACATGACCAACCGGGATGTCATCAGCTCTGTGGAGGAGGGGTACCGCCTGCCCG
CACCCATGGGCTGCCCCCACGCCCTGCACCAGCTCATGCTCGACTGTTGGCACAAGGACC
GGGCGCAGCGGCCTCGCTTCTCCCAGATTGTCAGTGTCCTCGATGCGCTCATCCGCAGCC
CTGAGAGTCTCAGGGCCACCGCCACAGTCAGCAGGTGCCCACCCCCTGCCTTCGTCCGGA
GCTGCTTTGACCTCCGAGGGGGCAGCGGTGGCGGTGGGGGCCTCACCGTGGGGGACTGGC
TGGACTCCATCCGCATGGGCCGGTACCGAGACCACTTCGCTGCGGGCGGATACTCCTCTC
TGGGCATGGTGCTACGCATGAACGCCCAGGACGTGCGCGCCCTGGGCATCACCCTCATGG
GCCACCAGAAGAAGATCCTGGGCAGCATTCAGACCATGCGGGCCCAGCTGACCAGCACCC
AGGGGCCCCGCCGGCACCTCTGATGTACAGCCAGCAGGGCCCAGGCAGCCACCAAGCCCA
CCCCAGGTCATGCCAGCGGCAGAGGACGTGAGGGGCTGGCAGCAGGCAGGGCGGCCCCAG
GCCTCTGCCCTCCTCTCAGGTGCTGGAGGAGCTGAAGGCTTCGCCACAGGACCTGGAGTT
ATCAGGGGTCAGGCGCCTGGGAAGGGGCCTTTGGTGGCCACCCTGGTGAGGACACCTGTC
CCCCAGGGCAGGCACCTTCTCTTTTCCAGAGCCTGGGGCCTCCACGTCACAGAGTCCAAC
AGGGACATCACTCGCCTGCCTCTGTGTGCGTGCATGTGTGTGTGTGGTGGGGGGTGTTCT
CACAAGGTCATGGGATCTCATGTGAACAGTGTGTGATCAAGTGTGTCCACCCCTTCGGGT
CTCAGCATGGACGTGTGCATGTTATGAGCGTGTGCTTATCCGTTAAGGCTGGAGGCACAT
GTGGGTGATGGTGGATGATGTGTCATGAATGAGGAGGTGTGTGAGCAGGGAACTCAGTGT
GACACCGCCAGGTCCAGCACCCATGGGGGCGGGGGAGAGGCTCACCCCTACGTCCCCCCA
CACCTGGAGGCTGGAGCCAGGGGCCACTTCTGAACTGCACCAGCACCAGGCCCACCCTCG
TCTCTGCCTGGGTGAGCCCACCCCGGCTGTATCTCAGGTCTGGGTCCTCCCTCTAGCCGA
GGAGGCCACCTGCAGCCTCCACCCGGCTCTCACCGCTGCTTCAACAGGAAAACAGGGTTC
CCGGTCAGTCCGGCTGGCCGCCTTCATGGAGGCATCATGGCAGAGCACATGAGATGTCCT
CAGCTGGGCTTGGCTGCCTGGCCAGGGCCGGGGGCTCAGCAGCCTCCTCTAGCCTCTGAT
GCCTTCCCTCCACGGCCCAGGTCTCCTCACTCAAAGTCCCTTCGCCAACCTTTCAATGCC
CAGCCCTGACACCTGCCCTTTGTCCCCCAGGCCTAGGATCAGGGACCAGAGGATCCTATC
TTCTCAGCACCCAGCCCACCCCTTCCTGTAGCATATGGGGAGACTAAGGCCTGGAGAGAG
GGGTGATGCCCCGTCCCAGGTTGCACTGCAACCAAGTGTCAGAGTCGGGGCTCCGGCCTC
CTGCCAAGGCTCTTGTCCCCATACACCATCCCACAAGGGCGCTGGGGGTGGAAGTGCCCT
GGAAGCCCCTCCCCTCTCACACTGACCTCCCCCCTTACGGCCCACCAGGGTATGTAAATA
TCTCTTTTCTACCATGTCAGAATATTTTTTCCTCACTCCTGACAATGCAAAAATGGTCTT
CAAAGCACATAAAAAGCACCCAGGGTGAGAAAGCCCCATCCCGGGGGCCGTTGGCAGGCA
GGGAAGCAGGAACCCCACCGTGTGCCCCCTGCCAGCCCCAGAGGGAGTGGCGAGCCCAGC
TGCCCAGCCCTGCCCCCCCTCCCCATAGCCAGCACAGCTATCCCGCGGGGACACCAGCAC
TGAGCCCCCTCTCCCTCCTGCAATAATTCGGGGAGTCTCAGCCCCATCCAGGTGCCGCGG
CCAGCTCTCTACACCTCTATATATTATATTACTATATAGCCGAGCTGTTCTTCCTTCCTA
TGGAAGTCGGAAACATGGTCAGAACACGATCTGGGGGGGGGATCCTGTCTTCCTCCCCAC
CCCACCCCACTCTTACCCAATTTCTGGGCTCTGGATCCTCACAGTCATGGAGGCACCGTG
GGCCTGGCACTTGCAAAAGTGTGGCCCCTCACTCTAGTGTGTGGTCCCTCTCAGGGTCCT
GGGGATCTGCCTCTCTGTGGTCTCCATCCTGACTCTTGAACTTACCCACAATAAGAATAA
ATTCTGCCTCATCTTT
Protein sequence predicted based on nucleotide sequence
MAPARGRLPPALWVVTAAAAAATCVSAARGEVNLLDTSTIHGDWGWLTYPAHGWDSINEV DESFQPIHTYQVCNVMSPNQNNWLRTSWVPRDGARRVYAEIKFTLRDCNSMPGVLGTCKE TFNLYYLESDRDLGASTQESQFLKIDTIAADESFTGADLGVRRLKLNTEVRSVGPLSKRG FYLAFQDIGACLAILSLRIYYKKCPAMVRNLAAFSEAVTGADSSSLVEVRGQCVRHSEER DTPKMYCSAEGEWLVPIGKCVCSAGYEERRDACVACELGFYKSAPGDQLCARCPPHSHSA APAAQACHCDLSYYRAALDPPSSACTRPPSAPVNLISSVNGTSVTLEWAPPLDPGGRSDI TYNAVCRRCPWALSRCEACGSGTRFVPQQTSLVQASLLVANLLAHMNYSFWIEAVNGVSD LSPEPRRAAVVNITTNQAAPSQVVVIRQERAGQTSVSLLWQEPEQPNGIILEYEIKYYEK DKEMQSYSTLKAVTTRATVSGLKPGTRYVFQVRARTSAGCGRFSQAMEVETGKPRPRYDT RTIVWICLTLITGLVVLLLLLICKKRHCGYSKAFQDSDEEKMHYQNGQAPPPVFLPLHHP PGKLPEPQFYAEPHTYEEPGRAGRSFTREIEASRIHIEKIIGSGDSGEVCYGRLRVPGQR DVPVAIKALKAGYTERQRRDFLSEASIMGQFDHPNIIRLEGVVTRGRLAMIVTEYMENGS LDTFLRTHDGQFTIMQLVGMLRGVGAGMRYLSDLGYVHRDLAARNVLVDSNLVCKVSDFG LSRVLEDDPDAAYTTTGGKIPIRWTAPEAIAFRTFSSASDVWSFGVVMWEVLAYGERPYW NMTNRDVISSVEEGYRLPAPMGCPHALHQLMLDCWHKDRAQRPRFSQIVSVLDALIRSPE SLRATATVSRCPPPAFVRSCFDLRGGSGGGGGLTVGDWLDSIRMGRYRDHFAAGGYSSLG MVLRMNAQDVRALGITLMGHQKKILGSIQTMRAQLTSTQGPRRHL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pANT7_cGST
Synonyms:
pANT7-cGST
Description:
with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial
Comments:
One in a series of pANT7 vectors made by A. Lau and N. Ramachandran for use with NAPPA arrays. Can be used for cell free expression of the gene insert using human IVTT from Thermo Fisher or rabbit reticulocyte systems
Size (bp):
5964
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Joshua LaBaer
Al Lau
Niroshan Ramachandran
Al Lau
Niroshan Ramachandran
Publications:
PMID:
15232106
Title: Self-assembling protein microarrays
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 bacterial origin of replication
3931
3249
death cassette
ccdB
ccdB death cassette present in 'empty vector' form only (replace with gene of interest)
1876
2085
primer
NAP138
NAP138 reverse sequencing primer 5? TGTTTCGCCATTTATCACCTTC 3?
2405
2384
primer
NAP150
Forward sequencing primer NAP150 5?-CCC ATT GTA TGG GAT CTG ATC-3?
388
408
promoter
T7
T7 transcriptional start sequence
5940
5964
recombination site
attR1
AttR1 site for recombination in empty vector
549
565
recombination site
attR2
AttR2 site for recombination in empty vector
2236
2251
selectable marker
CmR
chloramphenicol resistance
782
1438
selectable marker
AmpR
ampicillin resistance gene
4688
4029
tag
GST
Glutathione transferase (GST) tag
2282
2950
