Gene:
Gene Symbol:
SLC5A3
Gene Name:
solute carrier family 5 (sodium/myo-inositol cotransporter), member 3
Original Clone ID:
HsCD00302610
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pANT7_cGST
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
Identical to HsCD00731674
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00604152
Price:
Login for Pricing
UNAVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 2154nts
Open reading frame: 337 to 2490
GGTTGGTACAGTAGGCTTCACTAGACTTAGCTGCAACTCAGAATTTCTCCTCCAGCACCT
GAGTAAATGCTGATGGTCTTGTGGAGAGTGGATTAAGAGTACGAGCTAAGTTCTCAATCC
CAATTAAGAAGCGGAAAATTTAAACTGTCTTCTTCAAAGTTTATCACAACCACCACCATC
AAGACAGCAAACCAAAGGACAAAGACTTTGACCCTGCTGTGTTGCTCTGTGTAGTCCAGT
TCACGTATGGTTTACAGACTTGGCTGGGGTTACTAAAAATAAATAAAAAGTTGGACACTT
CTGTCATTGGAGCGCTATTATTCACAAGTTACCAGAATGAGAGCTGTACTGGACACAGCA
GACATTGCCATAGTGGCCCTGTATTTTATCCTGGTCATGTGCATTGGTTTTTTTGCCATG
TGGAAATCTAATAGAAGCACCGTGAGTGGATACTTCCTGGCGGGGCGCTCTATGACCTGG
GTAACAATTGGTGCCTCTCTGTTTGTGAGCAATATTGGGAGTGAGCACTTCATTGGGCTG
GCAGGATCTGGAGCTGCAAGTGGATTTGCAGTGGGCGCATGGGAATTCAATGCCTTACTG
CTTTTACAACTTCTGGGATGGGTTTTCATCCCAATTTACATCCGGTCAGGGGTATATACC
ATGCCTGAATACTTGTCCAAGCGATTTGGTGGCCATAGGATTCAGGTCTATTTTGCAGCC
TTGTCTCTGATTCTCTATATTTTCACCAAGCTCTCGGTGGATCTGTATTCGGGTGCCCTT
TTTATCCAGGAGTCTTTGGGTTGGAATCTTTATGTGTCTGTCATCCTGCTCATTGGCATG
ACTGCTTTGCTGACTGTCACCGGAGGCCTTGTTGCAGTGATCTACACAGACACTCTGCAG
GCTCTGCTCATGATCATTGGGGCACTTACACTTATGATTATTAGCATAATGGAGATTGGC
GGGTTTGAGGAAGTTAAGAGAAGGTACATGTTGGCCTCACCCGATGTCACTTCCATCTTA
TTGACATACAACCTTTCCAACACAAATTCTTGTAATGTCTCCCCTAAGAAAGAAGCCCTG
AAAATGCTGCGGAATCCAACAGATGAAGATGTTCCTTGGCCTGGATTCATTCTTGGGCAG
ACCCCAGCTTCAGTATGGTACTGGTGTGCTGACCAAGTCATCGTGCAGAGGGTCCTTGCA
GCCAAAAACATTGCTCATGCCAAAGGCTCTACTCTTATGGCTGGCTTCTTAAAGCTCCTG
CCAATGTTTATCATAGTTGTCCCAGGAATGATTTCCAGGATACTGTTTACTGATGATATA
GCTTGCATCAACCCAGAGCACTGCATGCTGGTGTGTGGAAGCAGAGCTGGTTGCTCCAAT
ATTGCTTACCCACGCCTGGTGATGAAGCTGGTTCCTGTGGGCCTTCGGGGTTTAATGATG
GCAGTGATGATTGCAGCTCTGATGAGTGACTTAGACTCTATCTTTAACAGTGCCAGTACC
ATATTCACCCTCGATGTGTACAAACTTATCCGCAAGAGCGCAAGCTCCCGGGAGTTAATG
ATTGTGGGGAGGATATTTGTGGCATTTATGGTGGTGATCAGCATAGCATGGGTGCCAATC
ATCGTGGAGATGCAAGGAGGCCAGATGTACCTTTACATTCAGGAGGTAGCAGATTACCTG
ACACCCCCAGTGGCAGCCTTGTTCCTGCTGGCAATTTTCTGGAAGCGCTGCAATGAACAA
GGGGCTTTCTATGGTGGAATGGCTGGCTTTGTTCTTGGAGCAGTCCGTTTGATACTGGCC
TTTGCCTACCGTGCCCCAGAATGTGACCAACCTGATAATAGGCCGGGCTTCATCAAAGAC
ATCCATTATATGTATGTGGCCACAGGATTGTTTTGGGTCACGGGACTCATTACTGTAATT
GTGAGCCTTCTCACACCACCTCCCACAAAGGAACAGATTCGAACCACCACCTTTTGGTCT
AAGAAGAACCTGGTGGTGAAGGAGAACTGCTCCCCAAAAGAGGAACCATACCAAATGCAA
GAAAAGAGCATTCTGAGATGCAGTGAGAATAATGAGACCATCAACCACATCATTCCCAAC
GGGAAATCTGAAGACAGCATTAAGGGCCTTCAGCCTGAAGATGTTAATCTGTTGGTAACC
TGCAGAGAGGAGGGCAACCCAGTGGCATCCTTAGGTCATTCAGAGGCAGAAACACCAGTT
GACGCTTACTCCAATGGGCAAGCAGCTCTCATGGGTGAGAAAGAGAGAAAGAAAGAAACG
GATGATGGAGGTCGGTACTGGAAGTTCATAGACTGGTTTTGTGGCTTTAAAAGTAAGAGC
CTCAGCAAGAGGAGTCTCAGAGACCTGATGGAAGAGGAGGCTGTTTGTTTACAGATGCTA
GAAGAGACTCGGCAAGTTAAAGTAATACTAAATATTGGACTTTTTGCTGTGTGTTCACTT
GGAATTTTCATGTTTGTTTATTTCTCCTTATGAACTTAAGGATATGGTGAGACACTAACT
TAAGACAATACTGACTGGTCTTTGGGGAAAAAAGTTATGTAACTGTGCATCTCTCAGGCA
TTGTTTACGCTGTAGGTTTTAGCCAAATTTTACTTAGCAGAAAATCATCTAATTACAAGA
CTTTATTTTCCCAGAGATGGATTAAAGTAAATCTTCAACTTAAGTGAAGCCAAACCTAAC
AGACTGAATTGTGCAAATGTGGTTTTAAATTTTGCATACCAAAGTAAGAAGAGACCAATT
ATTCTCACAGAGCACTTAGAGCAGAATATATGTTAAGTTACCATGAATTAAGGTATACTG
TCTGCACTGCCAAGTCTTGGCAGACCTTACCCTGAAGTAGAAGATTTGCTCATTTCTAAA
GTTTTTTTTCTGTCTCTGTAATCCCTCCTACCATTAAGAAAAACTTATTTCTTAGACATT
GTACAATCAGTTATGTACTGAAAATCGAATGTGCTTGTGTGATACTTGTTTCAGGACAAG
TTCATTTGCCAGGTTCATTTTGTTAGCATGAGCCTACGGATTCTGATTTCCCAAAGAAAG
AATGTTTTCCTGTAGGTATTTTTGTACCACCAGTATATGGAATGTTAGGGAAAAACTTTG
TTCCAGTTCCTTTTTTTTTTTCTTTCTACTTTCAAGTTTAAGTGAACCATACTGAAATGA
CCAACAAGTCTGCCTGTAAAGTTACATGTCATGATTGTGTTGTTAAATGATTATGGGGGA
GAAAATGAAGTAAATGTTGCTGATGATCCCCATATTTATTGATCATATTAAGGTTGTTTA
TATAGTTTGGAAATGACCAGCCCCCTAAGCAGTGTTTGATTAACTTATGCTAATCAGATG
ATTACTCATATATTCTGCTAATTTTCTAGCTTTATTCTTGTTATTTGGAAAAATTATTAG
CCAAATGCCTTCCTAGGTGGATCCAGTTGGAAGATATGTCCAGAAACCTGAAGAAAAATT
GACGCTGCCTTTGTGTGCTGGATTGCTCTACTTGATTAGATCATGATATATCAAGGTTGA
ATTTTTAGAGGGAAAATTTAATTCTGATATCTTATTGCATCCTTGATAAGTTTTTCCCTG
ATTTTTTTTTTCCTCAAAAGACTTTCCATCTGTACACAGCCTCTACATTTTTGTTGTAGT
GACTTAGAGCATAAGGATGTTTCAGTGCAAACTGGCCGTCGGTAACAGAAAACTCAGTGC
ATACTTTGCTGTTGTTAGGTTGTCAATATAGTCTTTCTGTAGGATGGATAGCATGTTTGA
GAGGTGCCAAACAAGAACTTTTGGGGTTAGTAGTGTGTCTTGTGGAGGGTATTACAGGAC
TGTGTAATTATAGGACTCTAACTTGACATGGCTTGGCACCCACTTGCAGCTAGTGGGTAC
AGGGTACAAAAGATGTTAGAGAAAAGCTCTACAGATTACGTACTTCTGTGTCTTCGTATG
CTCAACACTGTCCTTTGTCCTCCATGAAAGATGAAGGAAGCAAATTATGTATGTACTTTC
TTTGACCTTCTTTAATCTCTGATACTTTTTAGATTGCATGATTTTACTAGGCTTGTATTT
AGGGAAATTACTTTCATAAATACTTTTGTAGATTTTGAATCAAAACTCAGTCTTTTTAAT
TTTTTTGTAGTCTATAAACTAGTTTCATTATGATGGACTTGATTAGTCCAAAGTTAATTT
TAGAAATTGTCAGGTAGCATAGTGTCTTCCCATGATCAGGAGGCTTTCTGAAGGACTGAG
TCTGTAAATGAAAAAATAATTTATGTATGAATAGCATGTATTTCTGAAGAGCTTAGAGTG
CCTTGTAGAATTTTTTTCTCAATTTTATTCTTGAGGTTTATAATTTGGGGGCCAAATAGA
TAGAGCTCATCATTTTCTTGTTTGGAAGTTGAGGCTGCGACATGTCCAAGGTTATGAAGT
CTCTTTTGGGAAGAACAGAAACCAGGTCTCCAAATCTGGACTCATGGTTTGTTCAGATGT
GTCTGGACAAATGGTTGTCAATGTTTTGTCCTGTTTTTTCAAAGGAACTGTTCTTCCTTT
GGGACAACCTTTTGGTGTTTGGGAAAGTAATAAGATCTTGGATTTTTCAAATTAACATTA
AGTTGTAAGAACTAAAATTTTCTTTGAACCACATTACTGTGTAATTCACTGATAATTGAC
ATATTGGCTGGGCAGCCTATCTCTTCCATATCCAGCGTAAATGAATAGGAGGTGTTTGTG
ATTTTTTTTTTCTCCCTTTATTTAACATTGAGTCCTAGTAGTTTGGAGAATTAGGGTCCC
TCTACCTTCTTTCTGCTCTTGTCTTAGTAAGATACATAAGGTACATCATCTTGTGTCTGT
GTGTATATAGCAGTAGGTCAAGTTTAGAGTACTAAAGTCTGTAAATAAGGAATGACTATT
AGCATATTCATTAGAATTGTTTATTCTTGCCAGTATAAACATCATTTTATTTAGACTAAA
GTCCCTGAAGCTTGTCTTTCTTATTGCTTCCCAGTAATAGATAATGTGCTCGAGTAAGTT
TGTGAATTGCTGATTGCAACTTAATTCAGGGACCAGTCTTCAATCTATATTTCATTAGAA
TGATTGTTCCTGGAATGATCATACATGGACTGTCTTAAGCTAGCAAAATGTTCATACTTT
ACACTGACTAAATGGGTCCTAAATGATGACATTGGTCTTTAGACATTAACATGTGTATAT
TTTTATATTAGCTCAAGCTAAGGTTCAGAATTGAAGCTTGATATTGACTAGAATAGCTAA
AAGTCAAAATGAGGTGAGGACACTGGTCTTGGAAGGTAGAGAAAAATAAATGTCTTACCA
GGTGTTAATGGTATCCCCAGTTCTTAGACTTTTGTCTTCTCAGGCAATTTTCATCTCAAG
ATCTGATGAGAAGGGCATATTACATTGGTATGCAGGATGATTATTGCATATTTTGTGGGA
CCTCTAATTTCCCTGGTCATCTTTCAGAATATTCTGTTCTGCCACCCCCAGAGAGTAAAC
ACTTGAGCCGATTTCTTCTTCCCCAGCTATTCTTTCCTGGGGGTAATTATGCTTTGTCTT
TAGATTAGAGAAGCATCAAGCAATAGCAATGGTGCTGTGTCCTTCGGCCTAAATTCAATA
GATCTCATCTCCTAGGGCTTCCTTTTCACTTGGCTCAAAGGATCCATTGTATTTTGGCAC
AAAGAGCCTGGCCAGGGTCATGTAGCCATAGCTCTTAGGGATGATACCTCAAGAAATTAG
CTGGGACCCATCACTCTGTGAAACTTCACATTTTAAGAACTGAGTTGAGGGGGTTGTTAT
GCACTTCTGTAACTTGAGGCTAAGCAAGGGGTTAACTCTTGTGAGAGCCAATAGAGTGTG
TCTGTATTCGCAGTCCATGGCTCATTTTCTTTATAGTAGGCATATGGATCTTCCCCTCTG
ACTTTGAATATCATTTGGTGTGGCCTGTGGGTTATTTTCATTCTTTACCACCAAATAAAG
CGGCTTATTAGCTACTCAGTTACTTGCTACTCAAAGGTTAGGTCTTCCCTGTTCCTGCTT
GGCAGTGTTAAAGCTTACAGGGTTAACTTATGATGATTCTCCTGGCTCATTTTCATCAGA
GGCATGATGACTGGAAAGGGATCACATGGGTCGTTGGTGGTGACACCTCACTGTTTCCTA
GGTTTGGATAGAGAGATGTATACAAGACCTTTCCTGTTAAATTACGTGACTACAGAGACT
TGCCAGGACAAAATTTTCCTAAGAAATCAGAAAAATGATTAAGTGAGATAAGTACCTGGG
TGACACAGATATTAGCCCGTTGGTAAAAGACAACAAATATTAGCTTAAAATCTGCATATG
TAGAATCATTTTCATTAGATTTAGAGCTTGAAGCACCTTGGCTCTCAGCTACTTTAAACT
CCTCCCCATATAAATCAGGGCACCAATAAATAAGTTTCAGCTTTTTAAACCCTGGTTTGA
TGTTAAGCATTATAAAGTACGAAGTTTGTTACCACAGTAGAGATAATTTAGTAGAAAAAT
GCTTTGAGGCTTCAGTATTTGTAAGATTTTGCATTAGCCAGATGCTAGGTTGTTGAAGGC
ATTTCAGTGTTGATAATAGCCTGAGCAGACTTCTTTACAAATGGGATCTGTTTCTATATG
TGTATATGCCCACTTACCATTCAGAGAGACTGGTCTTTCTCTTTGTCTTCCTTCACATTG
CTGTGTCAGTTCTACACCTAGTCTTTTCAGCACTTAGCAAATTCAAATTTTGATTTTTTT
GTCAGCTTAGTTCACTTTAAGGCATATTGGCATGGTGTGTGAAAGTGATGTTTTGCCCCA
GTATTGAGGACTTTTAGATCCAAATAATGACTCATTAAATATAATTATGTTTTAAGTATA
CTGAATTTCTGTTAGCTTAAAATGTTAATTCTCAGGAATGATTTTCTCACACTTTGTGTT
GGCTAATAATAAAAGCACTGTTTTATTCTCAAAACTCCTTTTTCAAAAATTAGGGAGAGA
GCAGTAGTGATCATTTATGTGAGCCCCTTTGAAATGATGGTGTCAGAGTGCAGAGAAACA
ATGGAGTTTTGATGCCAAAAAGGTTTTTTTGCAGTAAAAGTAAAAATTTGGAATTAGTTG
GCATATAGAGGAACCCTTTTGTACTGGAACGTATGAGGCTGGATTGTGAAAAGGTAATCT
TTCGATTGCTAGACTTGGTTAACTTAGGGCTGCAAATCTTTTTCTTCTGTCAAGGTCACT
TAATATGGAATGTTTTTGTCAGACTGTCCTTTGTTGGAATACTTTAGCTGTTCAGCTACT
TTGACTCCTAGGAGAGAATTTAGTTAAGGTTCAAAGTAATTAACTGGCTTTGCCAGTGGT
GAGTCCCACACCATTATTCACTTAGTAGTCATATAAATGTTTTTATTTAAACTTCTCTCT
CTTCAATGCTGAGAATAAGGCTTTAAATTACTGATTCACCTTTAAAGGAATGTTGTGAGA
ATTGATGTAATTTCTGTTTCTGTTTCCATCTAAACTTCTTTATAAAAAGAGGGATTAGTT
TTTTTGTTTTGGGGTAAGCACCTAATTTATCCAGTAACCAACAACCCTAACCATTGGCAT
ATATAGTCTTTCACTCAGAAATAAACAAAAACTGTTTGGTATATCTGTATCATTGCTAAT
CTTGTGCACTTTACTTTTTGGGCAGTACCATACATAGTCTGAGGCTATTGACTTAAACCA
ATAACTGTACTTTATGTAATGACTCTTAAATTTGGTTACCTGGGTTCACAGCTTGCTTGA
AGAGAAAGGATGCTAGAATAAAGTAAGCAGCTGAAGAGCGAGCAAATCAAGACAAAACAC
AGTGGTCTCAGATTTTTCGTAGTGTGGGAACAGTGGTTTTGCTCTATACCACTGAAAAGC
ACTATAACATAATTGTTGTCCATGATACTGAAGCTTTTCCCCTCACTTCTAGGTTGTTTA
CATTCAGAGCTCTATCAATAAGAGGAATACATATTACAGTGAATTCGACAACCGCACAAG
TTGGCAGTAGGTATCCCCAACCTAATTTATCTTGGTAAATTCACCCTGTTTCCTAGTGCT
GCTGGATAAAAGAGTGTTTACTTTTTATTGCTCTTAGACAGAGTAGTCTAGATAAGTTTT
CAATTTATCAACATAGCCTAGACTTCTGTAAGTGGAATGTTCATTAGTAACTCATCTTTT
TGTTGTTATAATTGGAAACAGAAACGAGGCTTATTGCTATTGCAGAAATCCCAAACTGGC
AAAGGCCAGTATATATGGTATTCCATAATATAACCAGCTTTTGAAATTTATGTGTTTGGA
TTAGTGCCTTCTGGTTACCAGTATTGACTCTGCTAGTTTGCACCTTTCCGTTCTTAACAG
AAAATTTGTATTTGTTATTCCTCTTAAATTTTGTCGTAACTAGTGAAGGAAGTAAAAAAA
AAAAAAAAACATGCATTACATTGACATACTTTATGTGCAGCCTTTATTTAGGTTCAGTGA
AACCAGGTAGTTCTGTATTTGTGTTGTAGCCTAAATGTTGTTTCTTTTATATCCATTAAA
AACTTAAAGTTACTTATGTTCTGTGATCTTAATTTTGTTGTGTTTCCATTGTAGGTTGAT
AGGTATATCGAGAACAGGTACGTGACAACAGTTTATATTCCATGATAGAAAGCTAAAGTC
CATAGAAAGCACAAAATCGTGTTCACACATTAGTGTACCCACACATAGAAAGCACAAGAC
TAATAGTATTCTCTGTATCCCACAAGTGCCAGTCATAAAGGCCACCAGGTATTTGTCTCA
GAGTTGCTATGAGCACTACAGTATTGATAAGCCCAAGACAATGCGGTATCTAAACTGGTC
CTAATGGTAAGGGACCCAAAGGAATAATCTCAATAAGTTTGTACCACATTGATGGAGGGA
GAGAATATAAATGTCAAGAATGCCAAAATTATATTTGGGGGTTACTAGCTAAAATGGGGT
TTGAGGGCTTTTTACTGCAACTTGAAACTGGAGAAATAGGGACAGATGTCTAGGTTTTTG
GTGGGTGGAACAGGTGACATATTTCTGTTTTAAGCTGTAGTGTGATTGGGGTTTTTTGTA
AAAAATCTTAAATCTTTTAGGAAATATTACCTCTTAACAGTGCCCCCCCAAACATGCAGA
AAGTCATACTTTAACAGGGCAAATACTACTTGTCTTTGATTTTTTTTGTGTACGTTTGTA
TGTGAGAGATGAAGTTACCTTTATTTTTTTCCTATACTTGACTGTGCTTCATTTTAATAA
AGGATAATTTGATCTGAGTGTTCTGAGCATCAGACTAATTCTGAAGCATATTTGCTAGAG
GAGCTACTTTGCTTTTCACAATGGGGTGGAGAGGATTCTTTCACTTGTCCCATTAACCCT
CTTCTAGTCTAGATGAGATGAAATCTGTTAATGTGTGTGTAGAAGAAAACGTATGTTCTT
CTACTCAGCATTGCCCTTTTCCACCTCCTCACTTCACCTCCGAGTAGCTTGTTTATCAAG
AATGAATGAATGTCTTTGTCTTAAATTTTGCCCATGTGTTAAAAGATGTAATTCTCAGAA
TGGGAGAGAAATGACTACCTTTGTTCCTACTCTTTTATATAATTATCCTTTTAGGGAAAG
ACTTGGTCAACTCTAATATATCTAGAAGGAAGACTATATCTGGTGTAGACTAATATGAGA
TGTTTTAGAAGAGTTAACCTGAACACTTTGAGGGAGAGATTATTCTTGCCAGCAAAAAGC
TAGCCAGGAATGAGCCTACCACATTATTTGAGAATATCAAACCTCAGGCCTGGGGGGATG
AGGGGAAGAAGATTACCAGAAGTGCAGGAAAGAGAAGTTTGAGGAACACCCTTGGCTTAG
CAACATGTGATAATGCAAAGCTGTTATAACCTGTTAATCCTACGTACTATGTGTTCTGTA
CCTTTACATGTTTTTAAATTTAAGATAGTTTGTAAGAACTGTACAAAAAAATGCTTCTGG
AGATTTCTTTGGCAGAAATGCCTTTCATCTATAATTTCATGGAGAACTGCTTTAATTAGC
CTAGGTGAAAAGTAGTCCTAGCAGTGTAAATATGTATAATTAGAGTTTTCTAATTTCACT
GTGAGATCTCTAACTTTTGAGTGGCAAACAGATCAAGTCTTTTGCTCATAGACTTTTCTG
TGGGGTTATTAAAATGCAAAAGCTTTATTTTTTTTAATAATGCCATACTCCATTAGTGTC
AGATGATGGTATGGAATTTGTTCCCTTGCTTTCCCCCACTGTTACTGCTTCAGTTTATAG
ATTGCCAGCAGAGTTCAGAAATAGAGCAGGGATTTACCCGTTCTTTGCTTGGACATCCCA
TTTTCTTTTGTCCAGACCCATGTTGGCAATCATGTATGAACTGTGTTATACTTCTCAGTG
CTTTCTTTTTTCTTTTTGATAAGATGGATATCAAAAATAGTTGCTGTGCAAAAGTTAGTA
GTCTTCTTCAAGAAGAAAACCAATTCTTTTTCTAATAATATCCTGTGAAATTGCTTCATT
CATTCATTTATTTTTAAGCCAAATGTCAGCAGAGTGCTGCTGCTTTTATCTAGTAATTTT
GATATGTAAGTATTAATGCATTTTTAAAAGATGTCTACATTGAAACATGTTCTTCCCAGT
GTCCTGCTTATGATGCTTTGTTCAGATTTTTTGTAAGAGACCAGTTAGTACACTGGGGGT
GTATATTGTGTACATGTGTCATTTTAGTTAGGCATTGTAGGCCAAATGTGATTATAAATG
AAGTTGATGAACATTAATTTTGTTATTAGTGAGTTTTTTGAATTGTAAATGGATTTCCAG
TTTACCTTCTGTTGTCTACAGCTTTTTTAATTTTAAGGTTTGACTAATTGTATCCATCTC
ATTGTACAGTGTTTTAGTTGCAAGCAGAAAGTAGAATTTGGTATAAAGCAGGTTATTTCT
ATATTGAAAGGAGTACAGTTGAAATTGTAGATTTAAGATTGTTAAAATCATGACAATTCT
AACTTGTCTATTCTAACCTATTGTGTACAATCTGATTTTTTAAAATTGTAAACATGTATG
ATCTTGGTTTCATGTGTTTTTGAAAGTGTTATTGTTTAAAAAATGAAAAAAGCATATCTG
CTAAAGAGCTGTCAGTTTTCATTACTGACTCTGTAAAATACACTGTTCTTTGTGTACTGT
GTGTTATTTTGCCAGCTGCTGCATTAGCCTTCAAAAGTATTTGGAAACTTAAGATGAACT
ACATTTCTTGCAAAGTACATTCCTTTCTGTGGTATTTTGTCCTGTAACTGAAGTATAGTA
ATTATTTTATGGAAATGTTAGCAATTCTGTACCAACTTTGAATAAAATGAAAAATTTATA
Protein sequence predicted based on nucleotide sequence
MRAVLDTADIAIVALYFILVMCIGFFAMWKSNRSTVSGYFLAGRSMTWVTIGASLFVSNI GSEHFIGLAGSGAASGFAVGAWEFNALLLLQLLGWVFIPIYIRSGVYTMPEYLSKRFGGH RIQVYFAALSLILYIFTKLSVDLYSGALFIQESLGWNLYVSVILLIGMTALLTVTGGLVA VIYTDTLQALLMIIGALTLMIISIMEIGGFEEVKRRYMLASPDVTSILLTYNLSNTNSCN VSPKKEALKMLRNPTDEDVPWPGFILGQTPASVWYWCADQVIVQRVLAAKNIAHAKGSTL MAGFLKLLPMFIIVVPGMISRILFTDDIACINPEHCMLVCGSRAGCSNIAYPRLVMKLVP VGLRGLMMAVMIAALMSDLDSIFNSASTIFTLDVYKLIRKSASSRELMIVGRIFVAFMVV ISIAWVPIIVEMQGGQMYLYIQEVADYLTPPVAALFLLAIFWKRCNEQGAFYGGMAGFVL GAVRLILAFAYRAPECDQPDNRPGFIKDIHYMYVATGLFWVTGLITVIVSLLTPPPTKEQ IRTTTFWSKKNLVVKENCSPKEEPYQMQEKSILRCSENNETINHIIPNGKSEDSIKGLQP EDVNLLVTCREEGNPVASLGHSEAETPVDAYSNGQAALMGEKERKKETDDGGRYWKFIDW FCGFKSKSLSKRSLRDLMEEEAVCLQMLEETRQVKVILNIGLFAVCSLGIFMFVYFSL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pANT7_cGST
Synonyms:
pANT7-cGST
Description:
with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial
Comments:
One in a series of pANT7 vectors made by A. Lau and N. Ramachandran for use with NAPPA arrays. Can be used for cell free expression of the gene insert using human IVTT from Thermo Fisher or rabbit reticulocyte systems
Size (bp):
5964
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Joshua LaBaer
Al Lau
Niroshan Ramachandran
Al Lau
Niroshan Ramachandran
Publications:
PMID:
15232106
Title: Self-assembling protein microarrays
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 bacterial origin of replication
3931
3249
death cassette
ccdB
ccdB death cassette present in 'empty vector' form only (replace with gene of interest)
1876
2085
primer
NAP138
NAP138 reverse sequencing primer 5? TGTTTCGCCATTTATCACCTTC 3?
2405
2384
primer
NAP150
Forward sequencing primer NAP150 5?-CCC ATT GTA TGG GAT CTG ATC-3?
388
408
promoter
T7
T7 transcriptional start sequence
5940
5964
recombination site
attR1
AttR1 site for recombination in empty vector
549
565
recombination site
attR2
AttR2 site for recombination in empty vector
2236
2251
selectable marker
CmR
chloramphenicol resistance
782
1438
selectable marker
AmpR
ampicillin resistance gene
4688
4029
tag
GST
Glutathione transferase (GST) tag
2282
2950
