| Gene 1: |
Gene Symbol:
Light Chain Gene Name: Light Chain |
|---|---|
| Gene 2: |
Gene Symbol:
Heavy Chain Gene Name: Heavy Chain Sequence |
| Original Clone ID: | anti-PRDM11-RAB-C109 |
| Keyword: | anti-PRDM11-RAB-C109 recombinant antibody fab fragment |
| Species: | Homo sapiens |
| Type: | cDNA |
| Vector Name: | pSFV3 Format: FUSION |
| Source: | Recombinant Antibody Network |
| Description: | None |
| Comments: | None |
| Mutation/ Discrepancy : |
No / No |
| Publications: | None |
| Authors: |
The University of Chicago Recombinant Antibody Network Protein Capture Reagents Program |
|
HsCD00604274 Price: Login for Pricing AVAILABLE No restriction Special MTA: SPTA |
Insert sequence: 714nts
Open reading frame : 1 to 714 & 1 to 840
ATGAAATCCCTATTGCCTACGGCAGCCGCTGGATTGTTATTACTCGCGGCCCAGCCGGCC
ATGGCGTCCGATATCCAGATGACCCAGTCCCCGAGCTCCCTGTCCGCCTCTGTGGGCGAT
AGGGTCACCATCACCTGCCGTGCCAGTCAGTCCGTGTCCAGCGCTGTAGCCTGGTATCAA
CAGAAACCAGGAAAAGCTCCGAAGCTTCTGATTTACTCGGCATCCAGCCTCTACTCTGGA
GTCCCTTCTCGCTTCTCTGGTAGCCGTTCCGGGACGGATTTCACTCTGACCATCAGCAGT
CTGCAGCCGGAAGACTTCGCAACTTATTACTGTCAGCAATCTCATTACTCTCCGTTCACG
TTCGGACAGGGTACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC
TTCCCGCCATCTGATTCACAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAAT
AACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGT
AACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGC
ACCCTGACGCTGAGCAAAGCAGACTACGAAAAACATAAAGTCTACGCCTGCGAAGTCACC
CATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTAA
ATGAAAAAGAATATCGCATTTCTTCTTGCATCTATGTTCGTTTTTTCTATTGCTACAAAC
GCGTACGCTGAGATCTCCGAGGTTCAGCTGGTGGAGTCTGGCGGTGGCCTGGTGCAGCCA
GGGGGCTCACTCCGTTTGTCCTGTGCAGCTTCTGGCTTCAACATCTCTTCTTCTTATATG
CACTGGGTGCGTCAGGCCCCGGGTAAGGGCCTGGAATGGGTTGCATCTATTTCTCCTTAT
TATGGCTATACTTCTTATGCCGATAGCGTCAAGGGCCGTTTCACTATAAGCGCAGACACA
TCCAAAAACACAGCCTACCTACAAATGAACAGCTTAAGAGCTGAGGACACTGCCGTCTAT
TATTGTGCTCGCTACCATTACTACCCGGTTCCGTGGTACTGGGCTTTTGACTACTGGGGT
CAAGGAACCCTGGTCACCGTCTCCTCGGCCTCCACCAAGGGTCCATCGGTCTTCCCCCTG
GCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGAC
TACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGCCCTGACCAGCGGCGTGCAC
ACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTG
CCCTCCAGCAGCTTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGCCCAGCAAC
ACCAAGGTCGACAAGAAAGTTGAGCCCAAATCTTGTGACAAAACTCACACAGGAGGTTCT
GGCAGCGCCGGTGGCCTGAACGATATTTTTGAAGCGCAGAAAATTGAATGGCATGAATGA
|
| Coding Sequence Details | |
|---|---|
| Insert Sequence Verified?: Y | Verification Method: Sequence Verification |
|
Mutations: None |
Discrepancy: None |
| 5' Linker Sequence: None | |
| 3' Linker Sequence: None | |
| Distributed in bacterial strain : | DH5-alpha T1 phage resistant |
|---|---|
| Antibiotic Selection: |
Host Type:
bacterial
Marker:
ampicillin Bacterial Selection Condition: 100 ug/mL ampicillin Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended. Comments: Commonly used conditions for ampicillin resistant plasmid clones. |
| Protein Expression Results: |
ProteinExpressed: Protein_Confirmed ProteinPurified: Protein_Purified SolubleProtein: Protein_Soluble |
| Recommended expression in: | Not Applicable |
Powered by LabGenius |
||
| Vector Name: | pSFV3 | |
| Synonyms: | '' | |
| Description: | Bacterial expression vector for recombinant antibody expression with a Tac promoter and C-terminal 6xHis tag; ampicillin resistance in bacteria | |
| Comments: | None | |
| Size (bp): | 6775 | |
| Parent Vector: | None | |
| Empty Vector: | None | |
| Properties: | bacterial expression, loxP, with tag/fusion/marker | |
| Author Name: |
Structural Genomics Consortium |
|
| Publications: | None | |
| Vector Map: |
Vector Sequence:
|
|
| Sequence: |
Vector Features:
| Type | Name | Description | Start Position | End Position |
|---|---|---|---|---|
| bacterial origin | pBR322 origin | pBR322 origin | 1612 | 2231 |
| gene fragment | heavy chain | 5625 | 6470 | |
| gene fragment | light chain | Light chain | 4796 | 5506 |
| promoter | lac | lac promoter | 3171 | 3200 |
| promoter | lac 2 | Second lac promoter | 4497 | 4520 |
| promoter | tac promoter | Tac promoter | 4607 | 4635 |
| promoter | tac promoter 2 | Second Tav promoter | 4702 | 4730 |
| recombination site | LoxP | Lox P site | 4908 | 4938 |
| reporter gene | LacZ | Lac Z reporter gene | 2950 | 3109 |
| repressor protein gene | LacI | Lac I | 3249 | 4340 |
| selectable marker | AmpR | ampicillin resistance | 597 | 1457 |
| selectable marker | sacB | Sac B gene allowing negative selection on 5% sucrose | 4947 | 4882 |
| ssDNA origin | f1 | f1 origin | 29 | 335 |
| tag | His | C-terminal 6xHis tag | 6535 | 6615 |
| trxn termination sequence | TrpA | TrpA transcription terminator | 6657 | 6684 |
- Protein
- Clone
Protein
| PCRP Binder Details : | anti-PRDM11-RAB-C109 |
|---|
Clone
| Cloning Information : | 664555 |
|---|---|
| Original Clone ID : | anti-PRDM11-RAB-C109 |
