DNASU Plasmid | CRK (H. sapiens )<br>In pANT7_cGST (GST-tagged<br>In vitro expression vector)

Explanation of Terms

Gene:

Gene Symbol: CNGA1

Gene Name: cyclic nucleotide gated channel alpha 1

Original Clone ID:

HsCD00077212

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pANT7_cGST

Format:

FUSION

Source:

Center for Personalized Diagnostics

Description:

None

Comments:

Identical to HsCD00731249

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Center for Personalized Diagnostics

HsCD00616629

Price:

UNAVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 912nts

Open reading frame: 134 to 1045

Reference Sequence Alignment

CCCGCGGCTGCCGCCGCCATTTCGGGCGCTGCTGTGAAGCTGAAACCGGAGCCGGTCCGC
TGGGCGGCGGGCGCCGGGGGCCGGAGGGGCGCGCGCGGCGGCGGCACCCCAGCGTTTAGG
CGCGGAGGCAGCCATGGCGGGCAACTTCGACTCGGAGGAGCGGAGTAGCTGGTACTGGGG
GCGGTTGAGTCGGCAGGAGGCGGTGGCGCTGCTGCAGGGCCAGCGGCACGGGGTGTTCCT
GGTGCGGGACTCGAGCACCAGCCCCGGGGACTATGTGCTCAGCGTCTCAGAGAACTCGCG
CGTCTCCCACTACATCATCAACAGCAGCGGCCCGCGCCCGCCGGTGCCACCGTCGCCCGC
CCAGCCTCCGCCCGGGGTGAGCCCCTCCAGACTCCGAATAGGAGATCAAGAGTTTGATTC
ATTGCCTGCTTTACTGGAATTCTACAAAATACACTATTTGGACACTACAACGTTGATAGA
ACCAGTTTCCAGATCCAGGCAGGGTAGTGGAGTGATTCTCAGGCAGGAGGAGGCGGAGTA
TGTGCGAGCCCTCTTTGACTTTAATGGGAATGATGAGGAAGATCTTCCCTTTAAGAAAGG
AGACATCTTGAGAATCCGGGACAAGCCTGAAGAGCAGTGGTGGAATGCGGAGGACAGCGA
AGGCAAGAGAGGGATGATTCCAGTCCCTTACGTCGAGAAGTATAGACCTGCCTCCGCCTC
AGTATCGGCTCTGATTGGAGGTAACCAGGAGGGTTCCCACCCACAGCCACTGGGTGGGCC
GGAGCCTGGGCCCTATGCCCAACCCAGCGTCAACACTCCGCTCCCTAACCTCCAGAATGG
GCCCATATATGCCAGGGTTATCCAGAAGCGAGTCCCCAATGCCTACGACAAGACAGCCTT
GGCTTTGGAGGTCGGTGAGCTGGTAAAGGTTACGAAGATTAATGTGAGTGGTCAGTGGGA
AGGGGAGTGTAATGGCAAACGAGGTCACTTCCCATTCACACATGTCCGTCTGCTGGATCA
ACAGAATCCCGATGAGGACTTCAGCTGAGTATAGTTCAACAGTTTTGCTGACAGATGGGA
ACAATCTTTTTTTTTTTTTTCCAACTGCCATCTATACAATTTTCTTACAGATGTCAAAAG
CAGTCTAGTTTATATAAGCATTCTGTTACCTGTGATATTTTTTAGACTGAACTGCTCCAT
TCCTAGTCTTAATTACCATATTCAGGGTACGAACTGGAGGGCTTGTGTGTTAGCTTCTGA
ATTGGCAATTGGAGGCGGTAGTGGTCGTGCCTGTGTGTATCAGAAGGGATAGGTATCTTG
CCTCCTTTCTCTCAGGCAGTGCAAATCACCCTGTGGAAAACCGATGGACAGGAAGGAGTG
TTACACACTGCTTACCCTGATTTATTCAGTGGTTTTGTTTTCATTCTGGAACCATACTAT
CAAATGGCGACAGACTGTTCCGTTCCACCCCCGTGAAGTAATCATGCACCGTGTGAATAG
TATCAAGCAGGATTGCTTTCATTGTATGGAGCATGACCAGCGTGTGACTCATTCTGACAT
TTCAGATCCTAAGAATTCTAAGAACACTACTAGAAGCATTTGTTCCCTCCTAGTCAATGC
TTCATACTTTTTCTTGGGATTCTTTTAGCCCTTGACATTCTTGTCCCCCAAACCTGTAAG
TAGGTGAATTCCTAAGATAAGTGTGTATTTTCATTCCAGGTGAAAAGCAGGATGTACCGA
GCACTTTATTCAGTGCATAGCTTTAAGCCAGTGTTGGATTCACTAAGTGGACAGCCAGTC
TCCCAGCTCTCTGCCTTCCCCAAAAGGGTCGTAGTAGGTCACCCTTCTACAGCAGCTAAC
TAGAGTCCTAACTAATGGGATCCAGCAGGGCCATTTCTCCAGAGGGCCAGTATCCTATTA
GGAGACTCTTGGAATTCTTAGGTTCTACTCAAGAGTGGAAGGACCAATCACCTCTGATAT
TCTGTGGAAGGTTTTGGGGTCAAATTCTGCCCTCTGCATTCTGTGCAACTTGTATAAAAG
TCAAGTTAGTATTACATGAATTTGGGGTAGGGTTAGTGCTTTGAAAAAATGTTGAACCGG
CTGGGCGCGGTGGCTCACGTCTGTAATCCCAGCACTTTGGGAGGCCGAGGCGGGTGGATC
ATGAGGTCAGGAGTTCGAGACCAGCCTGGCCAACATAGTGAAACCCCATCTCTGCTAAAG
ATATAAAAAATTAGCCCGGCGTGGTGGTGCACGCCTGTAATCCCAGCTACTCGGGAGGCT
GAGGCAGGAGAATTGCTTCAACCTGGGAGGTGGAGGCTGCAGTGAGCCGAGATCGCACCA
CTGCGTTCCAGCCTGAGCGACAGGGCAAGACTCAGTCTCAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA

Protein sequence predicted based on nucleotide sequence

MAGNFDSEERSSWYWGRLSRQEAVALLQGQRHGVFLVRDSSTSPGDYVLSVSENSRVSHY
IINSSGPRPPVPPSPAQPPPGVSPSRLRIGDQEFDSLPALLEFYKIHYLDTTTLIEPVSR
SRQGSGVILRQEEAEYVRALFDFNGNDEEDLPFKKGDILRIRDKPEEQWWNAEDSEGKRG
MIPVPYVEKYRPASASVSALIGGNQEGSHPQPLGGPEPGPYAQPSVNTPLPNLQNGPIYA
RVIQKRVPNAYDKTALALEVGELVKVTKINVSGQWEGECNGKRGHFPFTHVRLLDQQNPD
EDFS

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pANT7_cGST

Synonyms:

pANT7-cGST

Sequencing Primer:

Forward: NAP150

Reverse: NAP138R

Description:

with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial

Comments:

One in a series of pANT7 vectors made by A. Lau and N. Ramachandran for use with NAPPA arrays. Can be used for cell free expression of the gene insert using human IVTT from Thermo Fisher or rabbit reticulocyte systems

Size (bp):

5964

Parent Vector:

None

Properties:

Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker

Author Name:

Joshua LaBaer
Al Lau
Niroshan Ramachandran

Publications:

PMID: 15232106

Title: Self-assembling protein microarrays

Vector Map:

Vector Sequence:

Sequence:

GGGCGAATTAATTCCGGTTATTTTCCACCATATTGCCGTCTTTTGGCAATGTGAGGGCCCGGAAACCTGGCCCTGTCTTCTTGACGAGCATTCCTAGGGGTCTTTCCCCTCTCGCCAAAGGAATGCAAGGTCTGTTGAATGTCGTGAAGGAAGCAGTTCCTCTGGAAGCTTCTTGAAGACAAACAACGTCTGTAGCGACCCTTTGCAGGCAGCGGAACCCCCCACCTGGCGACAGGTGCCTCTGCGGCCAAAAGCCACGTGTATAAGATACACCTGCAAAGGCGGCACAACCCCAGTGCCACGTTGTGAGTTGGATAGTTGTGGAAAGAGTCAAATGGCTCACCTCAAGCGTATTCAACAAGGGGCTGAAGGATGCCCAGAAGGTACCCCATTGTATGGGATCTGATCTGGGGCCTCGGTGCACATGCTTTACATGTGTTTAGTCGAGGTTAAAAAACGTCTAGGCCCCCCGAACCACGGGGACGTGGTTTTCCTTTGAAAAACACGATGATAATATGGATCGGATCCGAATTCGAGCTCCGTCATCAACAAGTTTGTACAAAAAAGCTGAACGAGAAACGTAAAATGATATAAATATCAATATATTAAATTAGATTTTGCATAAAAAACAGACTACATAATACTGTAAAACACAACATATCCAGTCACTATGGCGGCCGCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATAATGTGTGGATTTTGAGTTAGGATCCGTCGAGATTTTCAGGAGCTAAGGAAGCTAAAATGGAGAAAAAAATCACTGGATATACCACCGTTGATATATCCCAATGGCATCGTAAAGAACATTTTGAGGCATTTCAGTCAGTTGCTCAATGTACCTATAACCAGACCGTTCAGCTGGATATTACGGCCTTTTTAAAGACCGTAAAGAAAAATAAGCACAAGTTTTATCCGGCCTTTATTCACATTCTTGCCCGCCTGATGAATGCTCATCCGGAATTCCGTATGGCAATGAAAGACGGTGAGCTGGTGATATGGGATAGTGTTCACCCTTGTTACACCGTTTTCCATGAGCAAACTGAAACGTTTTCATCGCTCTGGAGTGAATACCACGACGATTTCCGGCAGTTTCTACACATATATTCGCAAGATGTGGCGTGTTACGGTGAAAACCTGGCCTATTTCCCTAAAGGGTTTATTGAGAATATGTTTTTCGTCTCAGCCAATCCCTGGGTGAGTTTCACCAGTTTTGATTTAAACGTGGCCAATATGGACAACTTCTTCGCCCCCGTTTTCACCATGGGCAAATATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGGCGATTCAGGTTCATCATGCCGTCTGTGATGGCTTCCATGTCGGCAGAATGCTTAATGAATTACAACAGTACTGCGATGAGTGGCAGGGCGGGGCGTAAAGATCTGGATCCGGCTTACTAAAAGCCAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGAATATATACTGATATGTATACCCGAAGTATGTCAAAAAGAGGTGTGCTATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCAATATCTCCGGTCTGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGAAATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACACGCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGGTGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTGGCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAAATGTCAGGCTCCCTTATACACAGCCAGTCTGCAGGTCGACCATAGTGACTGGATATGTTGTGTTTTACAGTATTATGTAGTCTGTTTTTTATGCAAAATCTAATTTAATATATTGATATTTATATCATTTTACGTTTCTCGTTCAGCTTTCTTGTACAAAGTGGTTGATGACAAGCTTGCGGCCGCACTCGAGCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCACTCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATGAGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTGGAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACAGTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTGGTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTGGATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAACTCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCGAAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCATCCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCCAATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTGAAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCATGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCCAAAATCGGATCTGGTTCCGCGTTGAAGATCTGACTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTTTAAACACTAGTCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAAGGAGGAACTATATCCGGGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGAAATTGTAAACGTTAATATTTTGTTAAAATTCGCGTTAAATTTTTGTTAAATCAGCTCATTTTTTAACCAATAGGCCGAAATCGGCAAAATCCCTTATAAATCAAAAGAATAGACCGAGATAGGGTTGAGTGTTGTTCCAGTTTGGAACAAGAGTCCACTATTAAAGAACGTGGACTCCAACGTCAAAGGGCGAAAAACCGTCTATCAGGGCGATGGCCCACTACGTGAACCATCACCCTAATCAAGTTTTTTGGGGTCGAGGTGCCGTAAAGCACTAAATCGGAACCCTAAAGGGAGCCCCCGATTTAGAGCTTGACGGGGAAAGCCGGCGAACGTGGCGAGAAAGGAAGGGAAGAAAGCGAAAGGAGCGGGCGCTAGGGCGCTGGCAAGTGTAGCGGTCACGCTGCGCGTAACCACCACACCCGCCGCGCTTAATGCGCCGCTACAGGGCGCGTCGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATTGTAATACGACTCACTATA

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ColE

ColE1 bacterial origin of replication

3931

3249

death cassette

ccdB

ccdB death cassette present in 'empty vector' form only (replace with gene of interest)

1876

2085

primer

NAP138

NAP138 reverse sequencing primer 5? TGTTTCGCCATTTATCACCTTC 3?

2405

2384

primer

NAP150

Forward sequencing primer NAP150 5?-CCC ATT GTA TGG GAT CTG ATC-3?

388

408

promoter

T7 transcriptional start sequence

5940

5964

recombination site

attR1

AttR1 site for recombination in empty vector

549

565

recombination site

attR2

AttR2 site for recombination in empty vector

2236

2251

selectable marker

CmR

chloramphenicol resistance

782

1438

selectable marker

AmpR

ampicillin resistance gene

4688

4029

tag

GST

Glutathione transferase (GST) tag

2282

2950

Gene

Pathways

Protein

Clone

Organism

Genome
Reagents

Gene

Gene Symbol :

CNGA1

Symbol Nomenclature :

CNGA1

SYNONYM :

CNCG; CNCG1; CNG-1; CNG1; RCNC1; RCNCa; RCNCalpha; RP49

Designation :

Full Nomenclature :

cyclic nucleotide gated channel alpha 1

GENEID :

1259

HGNC :

2148

MIM :

123825

Vega :

OTTHUMG00000160668

Target GenBank:

BC008506

Reference Sequence Alignment

  

HsCD00616629        -------CCCGCGGCTGCCGCCGCCATTTCGGGCGCTGCTGTGAAGCTGAAACCGGAG--
NM_001142564.1      ATGGAGTCTCGCTCTTCGCCCAGGCTGGAGTGCAGTGGCGCGATCTCGGCTCACTGCAGT
                           * ***   *  * *.* *:  :  * .*  **   .:. * *.:..* *..  

HsCD00616629        CCGGTCCGCTGGGCGGCGGGCGCCGGGGGCCGGAGGGGCGCGCGCGGCGGCGGCACCCCA
NM_001142564.1      CTTCACCTCCCAGATTCAAGCGATTTCCAGCTAATTTTTGTATTTTTAGTAG------AG
                    *   :** *  .*.  *..***.     . * .*     * .     .* .*      ..

HsCD00616629        GCGTTTAGGCGCGGAGGCAGCCATGGCGGGCAACTTCGACTCGGAGGAGCGGAGTAGCTG
NM_001142564.1      ATGGGATTTCACCATGTTGGCCAAGCTGG---ACTTGAACTCCTGATCTCAAGTGATCTG
                    . *  ::  *.* .:*  .****:*  **   **** .****  .. . *...  * ***

HsCD00616629        GTACTGGGGGCGGTTGAGTCGGCAGGAGGCGGTGGCGCTGCTGCAGGGCCAGCGGCACGG
NM_001142564.1      CCCACCTCAGCCTCCCAAAGTGCTG-GTATTACAGATATGAAACTATCCATGAAGAACAA
                      ..    .**     *.:  **:* . .  . .*. .**.:.*:.  *.:*..*.**..

HsCD00616629        GGTGTTCCTGGTGCGGGACTCG---AGCACCAGCCCCGGGGACTATGTGCTCAGCGTCTC
NM_001142564.1      TATTATCAATACACAGCAGTCTTTTGTAACCATGCCCAATGTGATTGTACCAGATATTGA
                     .* :**.: . .*.* * **    . .****  ***.. *: ::***.* ... .*  .

HsCD00616629        AGAGAACTCGCGCGTCTCCCACTACATCATCAACAGCAGCGGCCCGCGCCCGCCGGTGCC
NM_001142564.1      AAAGGAAATACGAAGGATGGAAAATGGAGCATGCAGCT-----CCTTTTCTGAGGATGAT
                    *.**.*.: .**..  :   *.:* . .. .:.****:     **    * *. *.**. 

HsCD00616629        ACCGTCGCCCGCCCAGCCTCCGCCCGGGGTGAGCCCCTCCAGACTCCGAATAGGAGATCA
NM_001142564.1      GACAGTGCCT---CTACATCTGAAGAATCAGAGAATGAAAACCCTCATGCAAGGGGTTCC
                    ..*.  ***    *:.*.** *.. ..  :***..  :..* .***. ..:***.*:**.

HsCD00616629        AGAGTTTGATTCATTGCCTGCTTTACTGGAATTCTACAAAATACACTATTTGGACACTAC
NM_001142564.1      TTTAGTTATAAGTCACTCAGAAAGGGAGGACCATCACAGAGGGAGCAGTACCTGCCTGGT
                    : :. **.::: : :  *:*.:: . :***. :  ***.*. ...*:.*:   .*.  . 

HsCD00616629        AACGTTGATAGAACCAGTTTCCAGATCCAGG-CAGGGTAGTGGAGTGATTCTCAGGCAGG
NM_001142564.1      GCCATTGCACTTTTTAATGTGAACAACAGCAGCAATAAGGACCAGGAACCAGAAGAAAAA
                    ..*.***.:. ::  *.* * .* *:*.. . **. .:.*:  ** .*  . .**..*..

HsCD00616629        AGGAGGCGGAGTATGTGCGAGCCCTCTTTGACTTTAATGGGAATGATGAGGAAGATCTTC
NM_001142564.1      AAGAAAAAGAAAAAAGAAAAGAAGAGCAAGTCAGATGATAAAAACGAAAATAAAAACGAC
                    *.**....**.:*:. ...**.. :  ::*:*: ::.: ..**: .:.*. **.*:* :*

HsCD00616629        CCTTTAAGAAAGGAGACATCTTGAGAATCCGGGACAAGCCTGAAGAGCAGTGGTGGAATG
NM_001142564.1      CCAGAGAAGAAAAAGAAGAAAAAGGACAAAGAGAAGAAAAAGAAAGAGGAGAAAAGCAAA
                    **: :.*..**..***..:.::..**.:..*.**..*...:***... .. ..:.*.*:.

HsCD00616629        CGGAGGACAGCGAAGGCAAGAGAGGGATGATTCCAGTCCCTTACGTCGAGAAGTATAGAC
NM_001142564.1      GATAAGAAAGAAGAGGAGAAGAAAGAAGTTGTGGTTATTGATCCCTCGG-GAAACACATA
                     . *.**.**...***..*...*.*.*  : *  : :   :*.* ***. .*.:.:..:.

HsCD00616629        CTGCCTCCGCCTCAGTATCGGCTCTGATTGGAGGTAACCAGGAGGGTTCCCACCCACAGC
NM_001142564.1      TTACAACTGGCTGTTTTGCATCACATTACCTGTTATGTACAACTGGACAATGGTTATTGC
                     *.*.:* * ** : *: *. *:*: ::   .  ::. ..... **: .. .   * :**

HsCD00616629        CACTGGGTGGGCCGGAGCCTGGGCCCTATGCCCAACCCAGCGTCAACACTCCGCTCCCTA
NM_001142564.1      CAGAGCATGTTTTGATGAACTTCAATCTGATTACCTAGAATATTGGCTCATTTTGGATTA
                    ** :* .**    *.:*..    ..  : .  ... . *. .* ..*:*:      . **

HsCD00616629        ACCTCCAGAATGGGCCCATATATGCCAGGGTTATCCAGAAGCGAGTCCCCAATGCCTACG
NM_001142564.1      CGTATCAGACATAGTCTATTTAATCGATATGTTTGTACGAACAAGGACAGGTTACCTAGA
                    .  : ****.: .* * **:**: * * .  *:*  * .*.*.** .*. .:*.**** .

HsCD00616629        ACAAGACAGCCTTGGCTTTGGAGGTCGGTGAGCTGGTAAAGGTTACGAAGATTAATGTGA
NM_001142564.1      ACAAGGACTGCTGGTAAAGGAAGAACT------------TAAACTCATAAATAAATATAA
                    *****...  ** * .:: *.**.:*             :..: :*.:*.**:***.*.*

HsCD00616629        GTGGTCAGTGGGAAGGGGAGTGTAATGGCAAACGAGGTCACTTCCCATTCACACATGTCC
NM_001142564.1      ATCCAACTTGCAATTTAAACTTGATGTTCTGTCACTGATACCAACTG-ATTTGCTGTATT
                    .*  :.. ** .*:  ..* *  *:   *:.:*.. *: ** :.* . : : .*:  :  

HsCD00616629        GTCTGCTGGATCAACAGAATCCCGATGAGGACTTCAGCTGAGTATAGTTCAACAGTTTTG
NM_001142564.1      TTAAGTTAGGGTGGAACTATCCAGAAATTAGATTAAACAGGTTGTTACGGTTCTCTCGTA
                     *.:* *.*.  ...* :****.**:.: ...**.*.*:*. *.*:.   ::*: *  *.

HsCD00616629        CTGACAGATGGGAACAATCTTTTTTTTTTTTTTCCAACTGCCATCTATACAATTTTCTTA
NM_001142564.1      TGTTTGAGTTCTTCCAGAGAACAGAAACAAGGACAAACTATCCAAACATCTTCAGGATTT
                       : ...*   :.**.: :: : ::: ::  :*.****. *.:.:.::*:: :  .**:

HsCD00616629        CAGATGTCAAAAGCAGTCTAGTTTATATAAGCATTCTGTTACCTGTG--ATATTTTTTAG
NM_001142564.1      CCAACCTTGTTATGTATATCGTCATCATTATCCACTGGAATGCATGTGTGTTCTACTCTA
                    *..*  * .::*  :.*.*.** :: **:* *.:   *::: *:     .*: *: * :.

HsCD00616629        ACTGAACTGCTCCATTCCTAGTCTTAATTACCATATTCAGGGTACGAACTGGAGGGCTTG
NM_001142564.1      TTTCTAAAGCTATTGGATTTGGAAATGATACATGGGTCTACCCTGATATTAATGATCCTG
                    : * :*.:***. :  . *:* .:::.:***.: . **:.   : .:* *..:*. * **

HsCD00616629        TGTGTTAGCTTCTG-----AATTGGCAATTGGAGGCGGTAGTGGTCGTGCCTGTGTGTAT
NM_001142564.1      AATTTGGCCGTTTGGCTAGAAAATACGTATACAGCCTTTACTGGTCTACACTGACTTTGA
                    :.* * . * * **     **:: .*.::*. ** *  ** ***** : .***: * *.:

HsCD00616629        CAGAAGGGATAGGTATCTTGCCTCCTTTCTCTCAGGCAGTGCAAATCACCCTGTGGAAAA
NM_001142564.1      CTACCATTGGTGAAACACCCCCTCCCGTGAGGGATTCTGAGTATGTCTTTGTGGTGGTTG
                    *:....  . :*.:* .   *****  * :   *  *:*:* *:.**:   **  *.::.

HsCD00616629        CCGATGGACAGGAAGGAGTGTTACACACTGCTTACCCTGATTTATTCAGTGGTTTTGTTT
NM_001142564.1      ATTTCCTAATTGGAGTGTTAATTTTTGCTACCATCGTTGGTAACATAGGTTCTATGATTT
                    .  :   *.: *.** . *.:*: : .**.* ::*  **.*::.:*..**  *:* .***

HsCD00616629        TCATTCTGGAACCATACTATCAAATGGCGACAGACTGTTCCGTTCCACCCCCGTGAAGTA
NM_001142564.1      CCAACATGAATGCAGCCAGAGCAGAATTTCAAGCAAGAATTGATGCTATCAAGCAATATA
                     **: .**.*: ** .*:.: .*.:.   ..**..:*::  *:* *:. *..* .*:.**

HsCD00616629        ATCATGCACCGTGTGAATAGTATCAAGCAGGATTGCTTTCATTGTATGGAGCATGACCAG
NM_001142564.1      TGCATTTTCGAAATGTAAGCAAAGATATGGAAAAGAGGGTTATTAAATGG----------
                    : ***  :* .:.**:*:. :*: *:. .*.*::*.    ::* :*: *.          

HsCD00616629        CGTGTGACTCATTCTGACATTTCAGATCCTAAGAATTCTAAGAACACTACTAGAAGCATT
NM_001142564.1      --------TTTGACTACCTGTGGACCAACAAAAAAACAGTTGATGAGAAAGAAGTCTTAA
                            * : :**..*: *  * .:.*:**.**: . ::**: * :*. *..:  :::

HsCD00616629        TGTTCCCTCCTAGTCAATGCTTCATACTTTTTCTTGGGATTCTTTTAGCCCTTGACATTC
NM_001142564.1      AGTATCTACCTG---------------ATAAACTAAGAGCAGAAATTGCCATCAACGTTC
                    :**: * :***.               :*:::**:.*.. : :::*:***.* .**.***

HsCD00616629        TTGTCCCCCAAACCTGTAAGTAGGTGAATTCCTAAGATAAGTGTGTATTTTCATTCCAGG
NM_001142564.1      ACTTAGACACATTAAAAAAGGTACGCATTTTTGCTGATTGTGAAGCTGGTCTGTTGGTGG
                    :  *. .*..*: .:.:*** :.   *:**   .:***:.  .:* :  *  .**  :**

HsCD00616629        TGAAAAGCAGGATGTACCGAGCACTTTATTCAGTGCATAGCTTTAAGCCAGTGTTGGATT
NM_001142564.1      AGTTGGTCTTGAAATTGCAACCCCAAGTCTACAGTCCTG--------GAGATTATATTTG
                    :*::.. *: **:.*: *.* *.*:: : *...  *.*.         ...* :*. :* 

HsCD00616629        CACTAAGTGGACAGCCAGTCTCCCAGCTCTCTGCCTTCCCCAAAAGGGTCGTAGTAGGTC
NM_001142564.1      CAAGAAAGGGGATATCGGACGAGAGATGTACATTATCAAGGAAGGCAAACTCGCTGTGGT
                    **. **. **..:. *.*:* . ...   :*:  .* ..  **.. ..:*  . *. *  

HsCD00616629        ACCCTTCTACAGCAGCTAACTAGAGTCCTAACTAATGGGATCCAGCAGGGCCATTTCTCC
NM_001142564.1      GGCAGATGATGGAGTCACTCAGTTTGTGGTATTGAGCGATGGCAGCTACTTCGGTGAGAT
                    . *. :  * .*.. *:.:*:. :     :* *.*  *.:  ****:.   *. * . . 

HsCD00616629        AGAGGGCCAGTATCCTATTAGGAGACTCTTGGAATTCTTAGGTTCTACTCAAGAGTGGAA
NM_001142564.1      CAGCATTCTTAACATTAAAGGGAGCAAAGCTGGCAATCGAAGAACGGCCAATATTAAAAG
                    ... .  *: :* . **::.****..:.   *..::   *.*::* .* .*:.: :..*.

HsCD00616629        GGACCAATCACCTCTGATATTCTGTGGAAGGTTTTGGGGTCAAATTCTGCCCTCTGCATT
NM_001142564.1      --TATTGGCTACTCAGACCTGTTCTG---------TCTCTCAAAAGATGACCTCATGG--
                      :. :. *:.***:** .*  * **             *****: .**.****:  .  

HsCD00616629        CTGTGCAACTTGTATAAAAGTCAAGTTAGTATTACATGAATTTGGGGTAGGGTTAGTGCT
NM_001142564.1      ---AAGCTCTAACTGAGTACCCAGATGCCAAAACTATGCTGGAAGAGAAAGGGAAGCAGA
                       :. .:**:. : *.:*  **..* . :*::. ***.:  :.*.*:*.** :** . :

HsCD00616629        TTGAAAAAATGTTGAACCGGCTGGGCGCGGTGGCTCACGTCTGTAATCCCAGCACTTTGG
NM_001142564.1      TTTTAATGAAAGATGGTCTACTGG-ATCTAAACATTGCAAATGCTGGCAGTGATCCTAAA
                    ** :**:.*:. : .. * .**** . * .:. .* .*.:.** :. *. :*.:* *:..

HsCD00616629        GAGGCCGAGGCGGGTGGATCATGAGGTCAGGAGTTCGAGACCAGCCTGGCCAACATAGTG
NM_001142564.1      GATCTTGAAGAGAAGGTTACTCGAATGGAGGGGTCAGTAGACCTCCTG-CAAACCAGGTT
                    **    **.*.*.. * ::*: **.   ***.** .*:...*. **** *.***.:.** 

HsCD00616629        AAACCCCATCTCTGCTAAAGATATAAAAAATTAGCCCGGCGTGGTGGTGCACGCCTGTAA
NM_001142564.1      TGCCCGAATCTTGGCTGAG-----TATGAGTCCATGCAGCAGAAACTGAAACAAAGATTA
                    :..** .****  ***.*.     :*:.*.* ..  *.**. ..:   ..**... .*:*

HsCD00616629        TCCCAGCTACTCGGGAGGCTGAGGCAGGAGAATTGCTTCAACCTGGGAGGTGGAGGCTGC
NM_001142564.1      ACCAAGGTTGAGAAATTTCTGAAACCGCTTATTGACACAGAATTTTCAAGTATTGAGGGA
                    :**.** *: : ...:  ****..*.* : *:* .*: ..*. *   *.**. :*.  *.

HsCD00616629        AGTGAGCCGAGATCGCACCACTGCGTTCCAGCCTGAGCGACAGGGCAAGACTCAGTCTCA
NM_001142564.1      CCTGGAGCGGAAAGTGGGCCCATCGACTCTACATAG------------------------
                    . **.. **..*:   . *.*: **:  *:.*.*..                        

HsCD00616629        AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
NM_001142564.1      ---------------------------------------------------

Pathways

NCI :

Alpha4 beta1 integrin signaling events

NCI :

Angiopoietin receptor Tie2-mediated signaling

NCI :

CXCR4-mediated signaling events

NCI :

E-cadherin signaling in the nascent adherens junction

NCI :

Endothelins

NCI :

EPHA forward signaling

NCI :

IGF1 pathway

NCI :

JNK signaling in the CD4+ TCR pathway

NCI :

LPA receptor mediated events

NCI :

Nectin adhesion pathway

NCI :

Neurotrophic factor-mediated Trk receptor signaling

NCI :

PDGFR-alpha signaling pathway

NCI :

PDGFR-beta signaling pathway

NCI :

RAC1 signaling pathway

NCI :

Signaling events mediated by focal adhesion kinase

NCI :

Signaling events mediated by Hepatocyte Growth Factor Receptor (c-Met)

NCI :

Signaling events mediated by PTP1B

NCI :

Signaling events regulated by Ret tyrosine kinase

NCI :

Urokinase-type plasminogen activator (uPA) and uPAR-mediated signaling

NCI :

VEGFR3 signaling in lymphatic endothelium

Panther :

Angiogenesis

Panther :

Integrin signalling pathway

Reactome :

ARMS-mediated activation

Reactome :

Cytokine Signaling in Immune system

Reactome :

Downstream signal transduction

Reactome :

Fcgamma receptor (FCGR) dependent phagocytosis

Reactome :

Hemostasis

Reactome :

Immune System

Reactome :

Innate Immune System

Reactome :

Insulin receptor signalling cascade

Reactome :

Integrin alphaIIb beta3 signaling

Reactome :

Interleukin-3, 5 and GM-CSF signaling

Reactome :

NGF signalling via TRKA from the plasma membrane

Reactome :

p130Cas linkage to MAPK signaling for integrins

Reactome :

Platelet activation, signaling and aggregation

Reactome :

Platelet Aggregation (Plug Formation)

Reactome :

Prolonged ERK activation events

Reactome :

Regulation of actin dynamics for phagocytic cup formation

Reactome :

Regulation of signaling by CBL

Reactome :

Signal attenuation

Reactome :

Signal Transduction

Reactome :

Signaling by Insulin receptor

Reactome :

Signaling by Interleukins

Reactome :

Signaling by PDGF

Reactome :

Signalling by NGF

Reactome :

Signalling to ERKs

WikiPathway :

angiogenesis overview

WikiPathway :

B Cell Receptor Signaling Pathway

WikiPathway :

EGF/EGFR Signaling Pathway

WikiPathway :

ErbB signaling pathway

WikiPathway :

Focal Adhesion

WikiPathway :

Insulin Signaling

WikiPathway :

Integrin-mediated cell adhesion

WikiPathway :

Kit receptor signaling pathway

WikiPathway :

MAPK signaling pathway

WikiPathway :

Regulation of Actin Cytoskeleton

WikiPathway :

Signaling of Hepatocyte Growth Factor Receptor

WikiPathway :

TCR Signaling Pathway

Protein

SMART domain :

SH3 : Src homology 3 domains

SMART domain :

SH2 : Src homology 2 domains

UniProt :

P46108

HPRD :

00445

PDBe Mol* Structure Viewer

Clone

Cloning Information :

637028

HIP Master Clone ID :

143204

IMAGE ID :

3452137

Original Clone ID :

FLH222595.01L

Original Clone ID :

HsCD00077212

Organism

Species Specific ID:

1398

Genome

Chromosome :

Map Location :

4p12

Ensembl :

ENSG00000198515

Reagents

Labome :

Crk-antibody

CRK (H. sapiens )In pANT7_cGST (GST-taggedIn vitro expression vector)

Coding Sequence Details

Recommended Growth Condition:

Vector Features:

Gene

Reference Sequence Alignment

Pathways

Protein

PDBe Mol* Structure Viewer

Clone

Organism

Genome

Reagents

CRK (H. sapiens )
In pANT7_cGST (GST-tagged
In vitro expression vector)