Gene:
Gene Symbol:
KCNJ2
Gene Name:
potassium inwardly-rectifying channel, subfamily J, member 2
Original Clone ID:
HsCD00302855
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pANT7_cGST
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
Identical to HsCD00731702
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00616697
Price:
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UNAVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 1281nts
Open reading frame: 384 to 1664
GCGCACTGGAGCCCTGGCCAGCGCGCAGCCTTCCCGGCGCCGGCGGGCTGGGTCTTGGGA
ATTCTGGTTTGCTTTGGCTCACTCGCTTTTTACAAACCACTGGATCTTACATGCCTCTGT
ACCCCCCACTTCCACTCCATGTCCCCATGCTCCTGCGCCAGCAACAGGACATGTTCTCTG
GATGTCAGCTGAGTCATTAAAGTAACTCTGCATGTCAGTAGACAGACCTTGGTAGAACCA
CAAGGCTCCCAGAGACACCCATCTCTCCTCATTTTTTTGGTGTGTGTGTCTTCACCGAAC
ATTCAAAACTGTTTCTCCAAAGCGTTTTGCAAAAACTCAGACTGTTTTCCAAAGCAGAAG
CACTGGAGTCCCCAGCAGAAGCGATGGGCAGTGTGCGAACCAACCGCTACAGCATCGTCT
CTTCAGAAGAAGACGGTATGAAGTTGGCCACCATGGCAGTTGCAAATGGCTTTGGGAACG
GGAAGAGTAAAGTCCACACCCGACAACAGTGCAGGAGCCGCTTTGTGAAGAAAGATGGCC
ACTGTAATGTTCAGTTCATCAATGTGGGTGAGAAGGGGCAACGGTACCTCGCAGACATCT
TCACCACGTGTGTGGACATTCGCTGGCGGTGGATGCTGGTTATCTTCTGCCTGGCTTTCG
TCCTGTCATGGCTGTTTTTTGGCTGTGTGTTTTGGTTGATAGCTCTGCTCCATGGGGACC
TGGATGCATCCAAAGAGGGCAAAGCTTGTGTGTCCGAGGTCAACAGCTTCACGGCTGCCT
TCCTCTTCTCCATTGAGACCCAGACAACCATAGGCTATGGTTTCAGATGTGTCACGGATG
AATGCCCAATTGCTGTTTTCATGGTGGTGTTCCAGTCAATCGTGGGCTGCATCATCGATG
CTTTCATCATTGGCGCAGTCATGGCCAAGATGGCAAAGCCAAAGAAGAGAAACGAGACTC
TTGTCTTCAGTCACAATGCCGTGATTGCCATGAGAGACGGCAAGCTGTGTTTGATGTGGC
GAGTGGGCAATCTTCGGAAAAGCCACTTGGTGGAAGCTCATGTTCGAGCACAGCTCCTCA
AATCCAGAATTACTTCTGAAGGGGAGTATATCCCTCTGGATCAAATAGACATCAATGTTG
GGTTTGACAGTGGAATCGATCGTATATTTCTGGTGTCCCCAATCACTATAGTCCATGAAA
TAGATGAAGACAGTCCTTTATATGATTTGAGTAAACAGGACATTGACAACGCAGACTTTG
AAATCGTGGTCATACTGGAAGGCATGGTGGAAGCCACTGCCATGACGACACAGTGCCGTA
GCTCTTATCTAGCAAATGAAATCCTGTGGGGCCACCGCTATGAGCCTGTGCTCTTTGAAG
AGAAGCACTACTACAAAGTGGACTATTCCAGGTTCCACAAAACTTACGAAGTCCCCAACA
CTCCCCTTTGTAGTGCCAGAGACTTAGCAGAAAAGAAATATATCCTCTCAAATGCAAATT
CATTTTGCTATGAAAATGAAGTTGCCCTCACAAGCAAAGAGGAAGACGACAGTGAAAATG
GAGTTCCAGAAAGCACTAGTACGGACACGCCCCCTGACATAGACCTTCACAACCAGGCAA
GTGTACCTCTAGAGCCCAGGCCCTTACGGCGAGAGTCGGAGATATGACTGACTGATTCCT
TCTCTGGAATAGTTACTTTACAACACGGTCTGTTGGTCAGAGGCCCAAAACAGTTATACA
GATGACGGTACTGGTCAAGATGGGTCAAGCAAGCGGCCACAAGGGACTGAGGCAAGCACA
ATGGTTTCAAAGAAAGACTGTAAGCTCCATGATTAGCATAAAGCACTAACCATGTCTCCA
TGTGACCCGATGGCACATAGATGTTGTAGAATAAGTTATGGGTTTTTATGTTTTGTTTTG
TGTTTTTCCAAAACTTGAACTTGCAGGCAAGCCTTGGTTGGGTATTTGATTTATCCAGAA
TGCTTCTCTTTAGGGAACAAGGATGTTTTTAATGGCATAACAAAGGCAAGACTCTGCCTT
AATTTTTGAAAAGCTGCTAACTACATGAACACAAATGTGTATTTTTGTTGCAGTGTAGTT
TTCCTTTTGTGTAATTTTAAAGTCAGTGTTGAATTTTATTGAAAGCTCATGATGCGCTTC
AAAGTGGCAAGTATTTGGCTATTAACTGCCAAAACAAGAGCCTGATTTTTTGAGGCCAGT
AATTCGTTTGCTAGAATTGATTTTTTTTCTCTCTCTCTTTGTTACATAAGGGCATTATGT
AACACTAGCCGAATGGTAGCCTCTGGGTTGTTGTTTTTTTCTTTTCCTCCATGATGTTAA
TGGGTTATCTCAAATTTTAAGTTAAACTACCTAAAATAAATACCAAAGATAATGCATATT
TTTGCACAGTGGAGCTTACACTTAAAAGAAAACAAAGCCCCATGGGCTGCCTTGAAATCA
AGAGACAATAACTTTGAACCTCAGCAAGACCTTGAACCGCCGGTTCATTTTGCACCTTAT
TCAGAAAATAGAGCATCATACTCACCGAGTCTAGTCAGTGTAGTGCTTTTAAAAATTTTG
TCCTTTCATGTAACTTTTTTATTTTAAGAGGAAGAAGAAGAAAGGGGCACACACACACAA
TACCGACGTCTATCCTTTCCTGCTAGGCAGTGCTGGCCAGGCTCATGTGTAGTGTGCGAG
ATGGTGATGTACTCTTATATTTTTCTGGGCTTTTCCTTTTGCACATTCCAAAATTCATTT
CATAAGACAAGATCTTCATAGGACCTCCTTGGCATCCTGGCATTCTCAAAACTGAGCCAT
CCAGCATGAAAGATAAATGGGTTTAAACCCTTGCTGCTGAATTTATTGCCTGGACTGTCA
GGACATCACCAGCCCACCTTCACCTTAGGGAAGATGCCACACCTGGCCTCCACACTTGCT
CTTCTGATCAGTCTGTCTGGATTGAGTCCTACAGTGTCAGATAGGGCGGCAAATGCCAAA
GCAGGGAAACAGGGAGGTGTGGACAAGCCAGTTTGATGCAGCACTTCAGATCAAGTGCTT
AGGAAGGAGAGGAAACTTGCCTTTTTTATGGCAGAGGATAGTAATGAAAATGTCTCAGTA
TTTTAGGGTCAATGAGAGCCATAAAAATATAACATAATCACAAGTAAAGGAGATAATGGT
CTAAAACAGCTATTTCCCTTTTCTGTGTGCATACTTATGACTGAATGTGAGCTAAGCATT
TTCTCCTGTGGAGCCCTAGAGCAGGTTACTAAGGAAGGACACATTGTTTTCCAGAAGCCT
CCCCTGCCTGGCTGACTGCCTTGCTAGAAACATAATTTTTTTTTTCTCACTGAAGCTCAA
TAATGGAACTCTTTTTTTTTTTTTTTTTAATTTAAAGTTCCCTATTTGTGAATTCTGGGA
TTACTGACTTTTCTTTTTAATTGGAGTCTCAAAATCAACTCTCTTATGGTATTATATCTC
TGTATGCCATTAAAAAACAGCTTGTTCTAGAATCATGTATTTTGTAAACTGATGTTTGTG
ATGGTCTCTGGTTCTTGAACAGCCATATCTGAATGCCGTGCCTGCAAAACTATGACAATT
TTTGCTGTTTTCAGCCTTCAGATTTGATGGCTTGGGAAACTGAGGTGTTATTTTCAATGA
AACAAAGAAAGAGATGTTAAGCAAGTGGTTGTTTTAGATCCAAATGTAAAGGCAGGTTTG
GGAAGGTGTTTAAAGAGTTGGAGGAATTGGGGATTGAGTTGTAAAGAAAACTTACAGAAG
AGGCAACAATTTGGTTCTTGACAGTGAGAGGATATTGAGGGCTTCAGCTGCTGCTATTAT
GATGTTTTGCAAAGGAAAATAATCAAACCAAAGAGTATTCAGTGATATGTAAATTAAATG
AAGATACAGTGGAGAATGGGGGTGACCACAAAAGAGGCTCCCCCTAAACACACAGTGCTG
CCACTTAAAAAGACTTGAGAAATTTGAAAGGGGGTGGGTATGGGGGGGGCAAGAAAGAGG
GAGGGAAATCTTTCAACTTATTTCTGAAAAAGAGAAAAAAATATAAAATTTCTGGTGCAC
AGGTTTGTTTTTTCAAGAAAATTTTGCAGAAGCTATGTTTTTAAAGTGTACATTTTATAA
AGTTTATCAGATATTTTCATATTTAAAGCCAAATGTAAATAGAGGTCTGTAAAGAAAAAT
AATTGCCATAGAAAGTATAATTTCAGTGCAGTAATTTCTGAGAGCTAGTACCTATATGCT
ACCGGTTAGCATGGTTTTAGCAAATATATACCAGCCTTATAAGGTTCGTATTGCTATGTT
CTTCTGTTATTTATTTCAGCATGGACTGTTCATTTGAAACCTTTTTCTAGTTATTAGCGT
TTTAACAGTTACAAGCTTTAAATGGCAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
TTGTCAAGAGCCAAGACACAGGTAATGCACGACATTGATTGCTGCATTTTACCTTCAAAA
TATTTGTCCTTATTGACTGGGTCTCCTTAATTAATGTACACATGTCATTAGAATGCAGAC
GGAGGGGACTCACCATGAATATCTGGGGTTGATTCCCAGATGTGTGTTGCTTCTCTATTG
CAAGCAGATTCCCTGTTGGATTTACTTCGGATTTATTCCCTTTTAAAGAATTTTTGCCCA
TATCTGGAAGGGCACTATATTTTTGGGAGGAGCCATAGATTCCTGGTTATCCTATTTTTA
AACAAAATGTAGACAAAGTGAACTCTATTTTGATTATTGAGAAAGGAGTAGTTTTCTATC
CCTCTAAGAGTATACTTGAATCAGACATTTTAAGGATGTCACTATGGCACTGTTGTCATT
TCCAAATTCCTAGAAAAGTTTGTTTTACTTTGTTTTTATTCTGTTAATGCATTCTTTCTT
CTCTTTACTTCCTTTCTTACCAGTACACTCCTATCTCAACTCTGTTTATTTGATGAGTTC
TGTCCCGTAAATCATATTTCCCTTACAATTAATAAATGTCACTTCATATTTTATAATAAA
CCACTCAGTAAAAGCAAAAGCTTGTCCTGAGAAGTAGAGTGAGTTCTTTTTCACTCTGTG
TCTAATAATGTTAAGGTGGGAAAAAAAAAAGTGTGGCATAGCTACCTGCCCATCCCCAAC
CCTCAGCAAAGTAGAATCTCTTTTCTGGTAATTTTGGGTTTCCGCTCTGGGCTCTGGCAA
GTTGAACAATCCTAGCCATTGACAATCGTGATAGTTATTATTTTCCCATTTGCTGTCTTT
TTGTATCTAAAGTCTTCCTATTGTACTGCACAAACCATGGATTGTACATATTTTTATATA
TTATGTCTTATTTTATTATTTCTAAATAAAAAAATTAAAAATTGAAAAAAAAAAAAA
Protein sequence predicted based on nucleotide sequence
MGSVRTNRYSIVSSEEDGMKLATMAVANGFGNGKSKVHTRQQCRSRFVKKDGHCNVQFIN VGEKGQRYLADIFTTCVDIRWRWMLVIFCLAFVLSWLFFGCVFWLIALLHGDLDASKEGK ACVSEVNSFTAAFLFSIETQTTIGYGFRCVTDECPIAVFMVVFQSIVGCIIDAFIIGAVM AKMAKPKKRNETLVFSHNAVIAMRDGKLCLMWRVGNLRKSHLVEAHVRAQLLKSRITSEG EYIPLDQIDINVGFDSGIDRIFLVSPITIVHEIDEDSPLYDLSKQDIDNADFEIVVILEG MVEATAMTTQCRSSYLANEILWGHRYEPVLFEEKHYYKVDYSRFHKTYEVPNTPLCSARD LAEKKYILSNANSFCYENEVALTSKEEDDSENGVPESTSTDTPPDIDLHNQASVPLEPRP LRRESEI
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pANT7_cGST
Synonyms:
pANT7-cGST
Description:
with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial
Comments:
One in a series of pANT7 vectors made by A. Lau and N. Ramachandran for use with NAPPA arrays. Can be used for cell free expression of the gene insert using human IVTT from Thermo Fisher or rabbit reticulocyte systems
Size (bp):
5964
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Joshua LaBaer
Al Lau
Niroshan Ramachandran
Al Lau
Niroshan Ramachandran
Publications:
PMID:
15232106
Title: Self-assembling protein microarrays
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 bacterial origin of replication
3931
3249
death cassette
ccdB
ccdB death cassette present in 'empty vector' form only (replace with gene of interest)
1876
2085
primer
NAP138
NAP138 reverse sequencing primer 5? TGTTTCGCCATTTATCACCTTC 3?
2405
2384
primer
NAP150
Forward sequencing primer NAP150 5?-CCC ATT GTA TGG GAT CTG ATC-3?
388
408
promoter
T7
T7 transcriptional start sequence
5940
5964
recombination site
attR1
AttR1 site for recombination in empty vector
549
565
recombination site
attR2
AttR2 site for recombination in empty vector
2236
2251
selectable marker
CmR
chloramphenicol resistance
782
1438
selectable marker
AmpR
ampicillin resistance gene
4688
4029
tag
GST
Glutathione transferase (GST) tag
2282
2950
