Gene:
Gene Symbol:
PLXNC1
Gene Name:
plexin C1
Original Clone ID:
HsCD00302901
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pANT7_cGST
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
Identical to HsCD00731713
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00616712
Price:
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UNAVAILABLE
No restriction
Special MTA: None
Special MTA: None
GCGAGGAGGAAACGGTGCCGGAGCGCGCAGGGCTTGCTGCCGCCACCGCCGCTGCACAGG
CTGCCGGAGCGAGCCTGCCGCGCGCCGCCCTCCCCGCTCTCCTTCCTGGGCGAGCTGCGG
GGATGGGGCGGCCGCGGGAGCCCGAGCGCGCGCAGGAACCGCCGCCGCCGCCGCCCGCGT
CTCCGTTGCCGCGCGCCTGAGCCGCCGTCGCCGCCGCGCGCCCTGCCCGGGGGCGGCCCC
CCCAGCCCCATGGAGGTCTCCCGGAGGAAGGCGCCGCCGCGCCCCCCGCGCCCCGCAGCG
CCACTGCCCCTGCTCGCCTATCTGCTGGCACTGGCGGCTCCCGGCCGGGGCGCGGACGAG
CCCGTGTGGCGGTCGGAGCAAGCCATCGGAGCCATCGCGGCGAGCCAGGAGGACGGCGTG
TTTGTGGCGAGCGGCAGCTGCCTGGACCAGCTGGACTACAGCCTGGAGCACAGCCTCTCG
CGCCTGTACCGGGACCAAGCGGGCAACTGCACAGAGCCGGTCTCGCTGGCGCCCCCCGCG
CGGCCCCGGCCCGGGAGCAGCTTCAGCAAGCTGCTGCTGCCCTACCGCGAGGGGGCGGCC
GGCCTCGGGGGGCTGCTGCTCACCGGCTGGACCTTCGACCGGGGCGCCTGCGAGGTGCGG
CCCCTGGGCAACCTGAGCCGCAACTCCCTGCGCAACGGCACCGAGGTGGTGTCGTGCCAC
CCGCAGGGCTCGACGGCCGGCGTGGTGTACCGCGCGGGCCGGAACAACCGCTGGTACCTG
GCGGTGGCCGCCACCTACGTGCTGCCTGAGCCGGAGACGGCGAGCCGCTGCAACCCCGCG
GCATCCGACCACGACACGGCCATCGCGCTCAAGGACACGGAGGGGCGCAGCCTGGCCACG
CAGGAGCTGGGGCGCCTCAAGCTGTGCGAGGGCGCGGGCAGCCTGCACTTCGTGGACGCC
TTTCTCTGGAACGGCAGCATCTACTTCCCCTACTACCCCTACAACTATACGAGCGGCGCT
GCCACCGGCTGGCCCAGCATGGCGCGCATCGCGCAGAGCACCGAGGTGCTGTTCCAGGGC
CAGGCATCCCTCGACTGCGGCCACGGCCACCCCGACGGCCGCCGCCTGCTCCTCTCCTCC
AGCCTAGTGGAGGCCCTGGACGTCTGGGCGGGAGTGTTCAGCGCGGCCGCTGGAGAGGGC
CAGGAGCGGCGCTCCCCCACCACCACGGCGCTCTGCCTCTTCAGAATGAGTGAGATCCAG
GCGCGCGCCAAGAGGGTCAGCTGGGACTTCAAGACGGCCGAGAGCCACTGCAAAGAAGGG
GATCAACCTGAAAGAGTCCAACCAATCGCATCATCTACCTTGATCCATTCCGACCTGACA
TCCGTTTATGGCACCGTGGTAATGAACAGGACTGTTTTATTCTTGGGGACTGGAGATGGC
CAGTTACTTAAGGTTATTCTTGGTGAGAATTTGACTTCAAATTGTCCAGAGGTTATCTAT
GAAATTAAAGAAGAGACACCTGTTTTCTACAAACTCGTTCCTGATCCTGTGAAGAATATC
TACATTTATCTAACAGCTGGGAAAGAGGTGAGGAGAATTCGTGTTGCAAACTGCAATAAA
CATAAATCCTGTTCGGAGTGTTTAACAGCCACAGACCCTCACTGCGGTTGGTGCCATTCG
CTACAAAGGTGCACTTTTCAAGGAGATTGTGTACATTCAGAGAACTTAGAAAACTGGCTG
GATATTTCGTCTGGAGCAAAAAAGTGCCCTAAAATTCAGATAATTCGAAGCAGTAAAGAA
AAGACTACAGTGACTATGGTGGGAAGCTTCTCTCCAAGACACTCAAAGTGCATGGTGAAG
AATGTGGACTCTAGCAGGGAGCTCTGCCAGAATAAAAGTCAGCCCAACCGGACCTGCACC
TGTAGCATCCCAACCAGAGCAACCTACAAAGATGTTTCAGTTGTCAACGTGATGTTCTCC
TTCGGTTCTTGGAATTTATCAGACAGATTCAACTTTACCAACTGCTCATCATTAAAAGAA
TGCCCAGCATGCGTAGAAACTGGCTGCGCGTGGTGTAAAAGTGCAAGAAGGTGTATCCAC
CCCTTCACAGCTTGCGACCCTTCTGATTATGAGAGAAACCAGGAACAGTGTCCAGTGGCT
GTCGAGAAGACATCAGGAGGAGGAAGACCCAAGGAGAACAAGGGGAACAGAACCAACCAG
GCTTTACAGGTCTTCTACATTAAGTCCATTGAGCCACAGAAAGTATCGACATTAGGGAAA
AGCAACGTGATAGTAACGGGAGCAAACTTTACCCGGGCATCGAACATCACAATGATCCTG
AAAGGAACCAGTACCTGTGATAAGGATGTGATACAGGTTAGCCATGTGCTAAATGACACC
CACATGAAATTCTCTCTTCCATCAAGCCGGAAAGAAATGAAGGATGTGTGTATCCAGTTT
GATGGTGGGAACTGCTCTTCTGTGGGATCCTTATCCTACATTGCTCTGCCACATTGTTCC
CTTATATTTCCTGCTACCACCTGGATCAGTGGTGGTCAAAATATAACCATGATGGGCAGA
AATTTTGATGTAATTGACAACTTAATCATTTCACATGAATTAAAAGGAAACATAAATGTC
TCTGAATATTGTGTGGCGACTTACTGCGGGTTTTTAGCCCCCAGTTTAAAGAGTTCAAAA
GTGCGCACGAATGTCACTGTGAAGCTGAGAGTACAAGACACCTACTTGGATTGTGGAACC
CTGCAGTATCGGGAGGACCCCAGATTCACGGGGTATCGGGTGGAATCCGAGGTGGACACA
GAACTGGAAGTGAAAATTCAAAAAGAAAATGACAACTTCAATATTTCCAAAAAAGACATT
GAAATTACTCTCTTCCATGGGGAAAATGGGCAATTAAATTGCAGTTTTGAAAATATTACT
AGAAATCAAGATCTTACCACCATCCTTTGCAAAATTAAAGGCATCAAGACTGCAAGCACC
ATTGCCAACTCTTCTAAGAAAGTTCGGGTCAAGCTGGGAAACCTGGAGCTCTACGTCGAG
CAGGAGTCAGTTCCTTCCACATGGTATTTTCTGATTGTGCTCCCTGTCTTGCTAGTGATT
GTCATTTTTGCGGCCGTGGGGGTGACCAGGCACAAATCGAAGGAGCTGAGTCGCAAACAG
AGTCAACAACTAGAATTGCTGGAAAGCGAGCTCCGGAAAGAGATACGTGACGGCTTTGCT
GAGCTGCAGATGGATAAATTGGATGTGGTTGATAGTTTTGGAACTGTTCCCTTCCTTGAC
TACAAACATTTTGCTCTGAGAACTTTCTTCCCTGAGTCAGGTGGCTTCACCCACATCTTC
ACTGAAGATATGCATAACAGAGACGCCAACGACAAGAATGAAAGTCTCACAGCTTTGGAT
GCCCTAATCTGTAATAAAAGCTTTCTTGTTACTGTCATCCACACCCTTGAAAAGCAGAAG
AACTTTTCTGTGAAGGACAGGTGTCTGTTTGCCTCCTTCCTAACCATTGCACTGCAAACC
AAGCTGGTCTACCTGACCAGCATCCTAGAGGTGCTGACCAGGGACTTGATGGAACAGTGT
AGTAACATGCAGCCGAAACTCATGCTGAGACGCACGGAGTCCGTCGTCGAAAAACTCCTC
ACAAACTGGATGTCCGTCTGCCTTTCTGGATTTCTCCGGGAGACTGTCGGAGAGCCCTTC
TATTTGCTGGTGACGACTCTGAACCAGAAAATTAACAAGGGTCCCGTGGATGTAATCACT
TGCAAAGCCCTGTACACACTTAATGAAGACTGGCTGTTGTGGCAGGTTCCGGAATTCAGT
ACTGTGGCATTAAACGTCGTCTTTGAAAAAATCCCGGAAAACGAGAGTGCAGATGTCTGT
CGGAATATTTCAGTCAATGTTCTCGACTGTGACACCATTGGCCAAGCCAAAGAAAAGATT
TTCCAAGCATTCTTAAGCAAAAATGGCTCTCCTTATGGACTTCAGCTTAATGAAATTGGT
CTTGAGCTTCAAATGGGCACACGACAGAAAGAACTTCTGGACATCGACAGTTCCTCCGTG
ATTCTTGAAGATGGAATCACCAAGCTAAACACCATTGGCCACTATGAGATATCAAATGGA
TCCACTATAAAAGTCTTTAAGAAGATAGCAAATTTTACTTCAGATGTGGAGTACTCGGAT
GACCACTGCCATTTGATTTTACCAGATTCGGAAGCATTCCAAGATGTGCAAGGAAAGAGA
CATCGAGGGAAGCACAAGTTCAAAGTAAAAGAAATGTATCTGACAAAGCTGCTGTCGACC
AAGGTGGCAATTCATTCTGTGCTTGAAAAACTTTTTAGAAGCATTTGGAGTTTACCCAAC
AGCAGAGCTCCATTTGCTATAAAATACTTTTTTGACTTTTTGGACGCCCAGGCTGAAAAC
AAAAAAATCACAGATCCTGACGTCGTACATATTTGGAAAACAAACAGCCTTCCTCTTCGC
TTCTGGGTAAACATCCTGAAGAACCCTCAGTTTGTCTTTGACATTAAGAAGACACCACAT
ATAGACGGCTGTTTGTCAGTGATTGCCCAGGCATTCATGGATGCATTTTCTCTCACAGAG
CAGCAACTAGGGAAGGAAGCACCAACTAATAAGCTTCTCTATGCCAAGGATATCCCAACC
TACAAAGAAGAAGTAAAATCTTATTACAAAGCAATCAGGGATTTGCCTCCATTGTCATCC
TCAGAAATGGAAGAATTTTTAACTCAGGAATCTAAGAAACATGAAAATGAATTTAATGAA
GAAGTGGCCTTGACAGAAATTTACAAATACATCGTAAAATATTTTGATGAGATTCTAAAT
AAACTAGAAAGAGAACGAGGGCTGGAAGAAGCTCAGAAACAACTCTTGCATGTAAAAGTC
TTATTTGATGAAAAGAAGAAATGCAAGTGGATGTAAGCACTCTGGGGCCTGGCTTAATCT
GGCAAAGTTCTTCAGACGACTTGGGAGCAAAATGGCTGCTTGAGCTACTCTGTGTCGTTA
ATTTGTTGTTTGCACATAGGTTCCACTTTGGGCACTGTCTTTTTAAGAGACCAAGGCACA
TGCACAGCTTTTAGAAAGCAA
Protein sequence predicted based on nucleotide sequence
MEVSRRKAPPRPPRPAAPLPLLAYLLALAAPGRGADEPVWRSEQAIGAIAASQEDGVFVA SGSCLDQLDYSLEHSLSRLYRDQAGNCTEPVSLAPPARPRPGSSFSKLLLPYREGAAGLG GLLLTGWTFDRGACEVRPLGNLSRNSLRNGTEVVSCHPQGSTAGVVYRAGRNNRWYLAVA ATYVLPEPETASRCNPAASDHDTAIALKDTEGRSLATQELGRLKLCEGAGSLHFVDAFLW NGSIYFPYYPYNYTSGAATGWPSMARIAQSTEVLFQGQASLDCGHGHPDGRRLLLSSSLV EALDVWAGVFSAAAGEGQERRSPTTTALCLFRMSEIQARAKRVSWDFKTAESHCKEGDQP ERVQPIASSTLIHSDLTSVYGTVVMNRTVLFLGTGDGQLLKVILGENLTSNCPEVIYEIK EETPVFYKLVPDPVKNIYIYLTAGKEVRRIRVANCNKHKSCSECLTATDPHCGWCHSLQR CTFQGDCVHSENLENWLDISSGAKKCPKIQIIRSSKEKTTVTMVGSFSPRHSKCMVKNVD SSRELCQNKSQPNRTCTCSIPTRATYKDVSVVNVMFSFGSWNLSDRFNFTNCSSLKECPA CVETGCAWCKSARRCIHPFTACDPSDYERNQEQCPVAVEKTSGGGRPKENKGNRTNQALQ VFYIKSIEPQKVSTLGKSNVIVTGANFTRASNITMILKGTSTCDKDVIQVSHVLNDTHMK FSLPSSRKEMKDVCIQFDGGNCSSVGSLSYIALPHCSLIFPATTWISGGQNITMMGRNFD VIDNLIISHELKGNINVSEYCVATYCGFLAPSLKSSKVRTNVTVKLRVQDTYLDCGTLQY REDPRFTGYRVESEVDTELEVKIQKENDNFNISKKDIEITLFHGENGQLNCSFENITRNQ DLTTILCKIKGIKTASTIANSSKKVRVKLGNLELYVEQESVPSTWYFLIVLPVLLVIVIF AAVGVTRHKSKELSRKQSQQLELLESELRKEIRDGFAELQMDKLDVVDSFGTVPFLDYKH FALRTFFPESGGFTHIFTEDMHNRDANDKNESLTALDALICNKSFLVTVIHTLEKQKNFS VKDRCLFASFLTIALQTKLVYLTSILEVLTRDLMEQCSNMQPKLMLRRTESVVEKLLTNW MSVCLSGFLRETVGEPFYLLVTTLNQKINKGPVDVITCKALYTLNEDWLLWQVPEFSTVA LNVVFEKIPENESADVCRNISVNVLDCDTIGQAKEKIFQAFLSKNGSPYGLQLNEIGLEL QMGTRQKELLDIDSSSVILEDGITKLNTIGHYEISNGSTIKVFKKIANFTSDVEYSDDHC HLILPDSEAFQDVQGKRHRGKHKFKVKEMYLTKLLSTKVAIHSVLEKLFRSIWSLPNSRA PFAIKYFFDFLDAQAENKKITDPDVVHIWKTNSLPLRFWVNILKNPQFVFDIKKTPHIDG CLSVIAQAFMDAFSLTEQQLGKEAPTNKLLYAKDIPTYKEEVKSYYKAIRDLPPLSSSEM EEFLTQESKKHENEFNEEVALTEIYKYIVKYFDEILNKLERERGLEEAQKQLLHVKVLFD EKKKCKWM
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pANT7_cGST
Synonyms:
pANT7-cGST
Description:
with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial
Comments:
One in a series of pANT7 vectors made by A. Lau and N. Ramachandran for use with NAPPA arrays. Can be used for cell free expression of the gene insert using human IVTT from Thermo Fisher or rabbit reticulocyte systems
Size (bp):
5964
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Joshua LaBaer
Al Lau
Niroshan Ramachandran
Al Lau
Niroshan Ramachandran
Publications:
PMID:
15232106
Title: Self-assembling protein microarrays
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 bacterial origin of replication
3931
3249
death cassette
ccdB
ccdB death cassette present in 'empty vector' form only (replace with gene of interest)
1876
2085
primer
NAP138
NAP138 reverse sequencing primer 5? TGTTTCGCCATTTATCACCTTC 3?
2405
2384
primer
NAP150
Forward sequencing primer NAP150 5?-CCC ATT GTA TGG GAT CTG ATC-3?
388
408
promoter
T7
T7 transcriptional start sequence
5940
5964
recombination site
attR1
AttR1 site for recombination in empty vector
549
565
recombination site
attR2
AttR2 site for recombination in empty vector
2236
2251
selectable marker
CmR
chloramphenicol resistance
782
1438
selectable marker
AmpR
ampicillin resistance gene
4688
4029
tag
GST
Glutathione transferase (GST) tag
2282
2950
