Gene:
Gene Symbol:
MINK1
Gene Name:
misshapen-like kinase 1
Original Clone ID:
FLH222620.01L
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pANT7_cGST
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
This clone is identical to HsCD00620942.
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00617695
Price:
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UNAVAILABLE
No restriction
Special MTA: None
Special MTA: None
GGCTGGCTCCGGGGAGATAGCGCCTGTCAGTCGGTGGGTCGGTCCTCGCGCCGGCCCTCC
CCCTCCCCGGTCTCCGGGGGAGGCGCGGTGGAGTCCGCCCCCGGGGTTCTCCGATGGGGG
AGAAGCGGCGACGGCGGCAGTGGAGTAACCGAGCCGGAGCGTGAGCGGCCCCGGTGCCCC
GTTCCCCACGGAGGCCATGGGCGACCCAGCCCCCGCCCGCAGCCTGGACGACATCGACCT
GTCCGCCCTGCGGGACCCTGCTGGGATCTTTGAGCTTGTGGAGGTGGTCGGCAATGGAAC
CTACGGACAGGTGTACAAGGGTCGGCATGTCAAGACGGGGCAGCTGGCTGCCATCAAGGT
CATGGATGTCACGGAGGACGAGGAGGAAGAGATCAAACAGGAGATCAACATGCTGAAAAA
GTACTCTCACCACCGCAACATCGCCACCTACTACGGAGCCTTCATCAAGAAGAGCCCCCC
GGGAAACGATGACCAGCTCTGGCTGGTGATGGAGTTCTGTGGTGCTGGTTCAGTGACTGA
CCTGGTAAAGAACACAAAAGGCAACGCCCTGAAGGAGGACTGTATCGCCTATATCTGCAG
GGAGATCCTCAGGGGTCTGGCCCATCTCCATGCCCACAAGGTGATCCATCGAGACATCAA
GGGGCAGAATGTGCTGCTGACAGAGAATGCTGAGGTCAAGCTAGTGGATTTTGGGGTGAG
TGCTCAGCTGGACCGCACCGTGGGCAGACGGAACACTTTCATTGGGACTCCCTACTGGAT
GGCTCCAGAGGTCATCGCCTGTGATGAGAACCCTGATGCCACCTATGATTACAGGAGTGA
TATTTGGTCTCTAGGAATCACAGCCATCGAGATGGCAGAGGGAGCCCCCCCTCTGTGTGA
CATGCACCCCATGCGAGCCCTCTTCCTCATTCCTCGGAACCCTCCGCCCAGGCTCAAGTC
CAAGAAGTGGTCTAAGAAGTTCATTGACTTCATTGACACATGTCTCATCAAGACTTACCT
GAGCCGCCCACCCACGGAGCAGCTACTGAAGTTTCCCTTCATCCGGGACCAGCCCACGGA
GCGGCAGGTCCGCATCCAGCTTAAGGACCACATTGACCGATCCCGGAAGAAGCGGGGTGA
GAAAGAGGAGACAGAATATGAGTACAGCGGCAGCGAGGAGGAAGATGACAGCCATGGAGA
GGAAGGAGAGCCAAGCTCCATCATGAACGTGCCTGGAGAGTCGACTCTACGCCGGGAGTT
TCTCCGGCTCCAGCAGGAAAATAAGAGCAACTCAGAGGCTTTAAAACAGCAGCAGCAGCT
GCAGCAGCAGCAGCAGCGAGACCCCGAGGCACACATCAAACACCTGCTGCACCAGCGGCA
GCGGCGCATAGAGGAGCAGAAGGAGGAGCGGCGCCGCGTGGAGGAGCAACAGCGGCGGGA
GCGGGAGCAGCGGAAGCTGCAGGAGAAGGAGCAGCAGCGGCGGCTGGAGGACATGCAGGC
TCTGCGGCGGGAGGAGGAGCGGCGGCAGGCGGAGCGCGAGCAGGAATACAAGCGGAAGCA
GCTGGAGGAGCAGCGGCAGTCAGAACGTCTCCAGAGGCAGCTGCAGCAGGAGCATGCCTA
CCTCAAGTCCCTGCAGCAGCAGCAACAGCAGCAGCAGCTTCAGAAACAGCAGCAGCAGCA
GCTCCTGCCTGGGGACAGGAAGCCCCTGTACCATTATGGTCGGGGCATGAATCCCGCTGA
CAAACCAGCCTGGGCCCGAGAGGTAGAAGAGAGAACAAGGATGAACAAGCAGCAGAACTC
TCCCTTGGCCAAGAGCAAGCCAGGCAGCACGGGGCCTGAGCCCCCCATCCCCCAGGCCTC
CCCAGGGCCCCCAGGACCCCTTTCCCAGACTCCTCCTATGCAGAGGCCGGTGGAGCCCCA
GGAGGGACCGCACAAGAGCCTGGTGGCACACCGGGTCCCACTGAAGCCATATGCAGCACC
TGTACCCCGATCCCAGTCCCTGCAGGACCAGCCCACCCGAAACCTGGCTGCCTTCCCAGC
CTCCCATGACCCCGACCCTGCCATCCCCGCACCCACTGCCACGCCCAGTGCCCGAGGAGC
TGTCATCCGCCAGAATTCAGACCCCACCTCTGAAGGACCTGGCCCCAGCCCGAATCCCCC
AGCCTGGGTCCGCCCAGATAACGAGGCCCCACCCAAGGTGCCTCAGAGGACCTCATCTAT
CGCCACTGCCCTTAACACCAGTGGGGCCGGAGGGTCCCGGCCAGCCCAGGCAGTCCGTGC
CAGACCTCGCAGCAACTCCGCCTGGCAAATCTATCTGCAAAGGCGGGCAGAGCGGGGCAC
CCCAAAGCCTCCAGGGCCCCCTGCTCAGCCCCCTGGCCCGCCCAACGCCTCTAGTAACCC
CGACCTCAGGAGGAGCGACCCTGGCTGGGAACGCTCGGACAGCGTCCTTCCAGCCTCTCA
CGGGCACCTCCCCCAGGCTGGCTCACTGGAGCGGAACCGCGTGGGAGCCTCCTCCAAACT
GGACAGCTCCCCTGTGCTCTCCCCTGGGAATAAAGCCAAGCCCGACGACCACCGCTCACG
GCCAGGCCGGCCCGCAGACTTTGTGTTGCTGAAAGAGCGGACTCTGGACGAGGCCCCTCG
GCCTCCCAAGAAGGCCATGGACTACTCGTCGTCCAGCGAGGAGGTGGAAAGCAGTGAGGA
CGACGAGGAGGAAGGCGAAGGCGGGCCAGCAGAGGGGAGCAGAGATACCCCTGGGGGCCG
CAGCGATGGGGATACAGACAGCGTCAGCACCATGGTGGTCCACGACGTCGAGGAGATCAC
CGGGACCCAGCCCCCATACGGGGGCGGCACCATGGTGGTCCAGCGCACCCCTGAAGAGGA
GCGGAACCTGCTGCATGCTGACAGCAATGGGTACACAAACCTGCCTGACGTGGTCCAGCC
CAGCCACTCACCCACCGAGAACAGCAAAGGCCAAAGCCCACCCTCGAAGGATGGGAGTGG
TGACTACCAGTCTCGTGGGCTGGTAAAGGCCCCTGGCAAGAGCTCGTTCACGATGTTTGT
GGATCTAGGGATCTACCAGCCTGGAGGCAGTGGGGACAGCATCCCCATCACAGCCCTAGT
GGGTGGAGAGGGCACTCGGCTCGACCAGCTGCAGTACGACGTGAGGAAGGGTTCTGTGGT
CAACGTGAATCCCACCAACACCCGGGCCCACAGTGAGACCCCTGAGATCCGGAAGTACAA
GAAGCGATTCAACTCCGAGATCCTCTGTGCAGCCCTTTGGGGGGTCAACCTGCTGGTGGG
CACGGAGAACGGGCTGATGTTGCTGGACCGAAGTGGGCAGGGCAAGGTGTATGGACTCAT
TGGGCGGCGACGCTTCCAGCAGATGGATGTGCTGGAGGGGCTCAACCTGCTCATCACCAT
CTCAGGGAAAAGGAACAAACTGCGGGTGTATTACCTGTCCTGGCTCCGGAACAAGATTCT
GCACAATGACCCAGAAGTGGAGAAGAAGCAGGGCTGGACCACCGTGGGGGACATGGAGGG
CTGCGGGCACTACCGTGTTGTGAAATACGAGCGGATTAAGTTCCTGGTCATCGCCCTCAA
GAGCTCCGTGGAGGTGTATGCCTGGGCCCCCAAACCCTACCACAAATTCATGGCCTTCAA
GTCCTTTGCCGACCTCCCCCACCGCCCTCTGCTGGTCGACCTGACAGTAGAGGAGGGGCA
GCGGCTCAAGGTCATCTATGGCTCCAGTGCTGGCTTCCATGCTGTGGATGTCGACTCGGG
GAACAGCTATGACATCTACATCCCTGTGCACATCCAGAGCCAGATCACGCCCCATGCCAT
CATCTTCCTCCCCAACACCGACGGCATGGAGATGCTGCTGTGCTACGAGGACGAGGGTGT
CTACGTCAACACGTACGGGCGCATCATTAAGGATGTGGTGCTGCAGTGGGGGGAGATGCC
TACTTCTGTGGCCTACATCTGCTCCAACCAGATAATGGGCTGGGGTGAGAAAGCCATTGA
GATCCGCTCTGTGGAGACGGGCCACCTCGACGGGGTCTTCATGCACAAACGAGCTCAGAG
GCTCAAGTTCCTGTGTGAGCGGAATGACAAGGTGTTTTTTGCCTCAGTCCGCTCTGGGGG
CAGCAGCCAAGTTTACTTCATGACTCTGAACCGTAACTGCATCATGAACTGGTGACGGGG
CCCTGGGCTGGGGCTGTCCCACACTGGACCCAGCTCTCCCCCTGCAGCCAGGCTTCCCGG
GCCGCCCCTCTTTCCCCTCCCTGGGCTTTTGCTTTTACTGGTTTGATTTCACTGGAGCCT
GCTGGGAACGTGACCTCTGACCCCTGATGCTTTCGTGATCACGTGACCATCCTCTTCCCC
AACATGTCCTCTTCCCAAAACTGTGCCTGTCCCCAGCTTCTGGGGAGGGACACAGCTTCC
CCTTCCCAGGAATTGAGTGGGCCTAGCCCCTCCCCCCTTTTCTCCATTTGAGAGGAGAGT
GCTTGGGGCTTGAACCCCTTACCCCACTGCTGCTGACTGGGCAGGGCCCTGGACCCCTTT
ATTTGCACGTCAGGGGAGCCGGCTCCCCCCTTGAATGTACCAGACCCTGGGGGGGGTCAC
TGGGCCCTAGATTTTTGGGGGGTCACCAGCCACTCCAGGGGCAGGGACCATTTCTTCATT
TTCTGAAAGCACTTTAATGATTCCCCTTCCCCCAAACTCCAGGGAATGGAGGGGGGACCC
CGCCAGCCAAAACATTCCCCCCATTCCCGACCCCCATCTCCTCTTCTAGCCCATGCCCTT
CCCCGGTGGAGGGAGGGAGCAGGGAGCCCTCACTCTCCACGCCCCTTGCTTGCATCTGTA
TATAGTGTGAGCAGCAAGTAACCCTTCTCCTCCCTCCCCCCTCACCCCTCCTCAATGTAG
TGGCCTTGGATATCCTGTTTGTTAATAAAGACAATTCAACCAGCAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Protein sequence predicted based on nucleotide sequence
MGDPAPARSLDDIDLSALRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTE DEEEEIKQEINMLKKYSHHRNIATYYGAFIKKSPPGNDDQLWLVMEFCGAGSVTDLVKNT KGNALKEDCIAYICREILRGLAHLHAHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDR TVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDIWSLGITAIEMAEGAPPLCDMHPMR ALFLIPRNPPPRLKSKKWSKKFIDFIDTCLIKTYLSRPPTEQLLKFPFIRDQPTERQVRI QLKDHIDRSRKKRGEKEETEYEYSGSEEEDDSHGEEGEPSSIMNVPGESTLRREFLRLQQ ENKSNSEALKQQQQLQQQQQRDPEAHIKHLLHQRQRRIEEQKEERRRVEEQQRREREQRK LQEKEQQRRLEDMQALRREEERRQAEREQEYKRKQLEEQRQSERLQRQLQQEHAYLKSLQ QQQQQQQLQKQQQQQLLPGDRKPLYHYGRGMNPADKPAWAREVEERTRMNKQQNSPLAKS KPGSTGPEPPIPQASPGPPGPLSQTPPMQRPVEPQEGPHKSLVAHRVPLKPYAAPVPRSQ SLQDQPTRNLAAFPASHDPDPAIPAPTATPSARGAVIRQNSDPTSEGPGPSPNPPAWVRP DNEAPPKVPQRTSSIATALNTSGAGGSRPAQAVRARPRSNSAWQIYLQRRAERGTPKPPG PPAQPPGPPNASSNPDLRRSDPGWERSDSVLPASHGHLPQAGSLERNRVGASSKLDSSPV LSPGNKAKPDDHRSRPGRPADFVLLKERTLDEAPRPPKKAMDYSSSSEEVESSEDDEEEG EGGPAEGSRDTPGGRSDGDTDSVSTMVVHDVEEITGTQPPYGGGTMVVQRTPEEERNLLH ADSNGYTNLPDVVQPSHSPTENSKGQSPPSKDGSGDYQSRGLVKAPGKSSFTMFVDLGIY QPGGSGDSIPITALVGGEGTRLDQLQYDVRKGSVVNVNPTNTRAHSETPEIRKYKKRFNS EILCAALWGVNLLVGTENGLMLLDRSGQGKVYGLIGRRRFQQMDVLEGLNLLITISGKRN KLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDMEGCGHYRVVKYERIKFLVIALKSSVEV YAWAPKPYHKFMAFKSFADLPHRPLLVDLTVEEGQRLKVIYGSSAGFHAVDVDSGNSYDI YIPVHIQSQITPHAIIFLPNTDGMEMLLCYEDEGVYVNTYGRIIKDVVLQWGEMPTSVAY ICSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVY FMTLNRNCIMNW
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pANT7_cGST
Synonyms:
pANT7-cGST
Description:
with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial
Comments:
One in a series of pANT7 vectors made by A. Lau and N. Ramachandran for use with NAPPA arrays. Can be used for cell free expression of the gene insert using human IVTT from Thermo Fisher or rabbit reticulocyte systems
Size (bp):
5964
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Joshua LaBaer
Al Lau
Niroshan Ramachandran
Al Lau
Niroshan Ramachandran
Publications:
PMID:
15232106
Title: Self-assembling protein microarrays
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 bacterial origin of replication
3931
3249
death cassette
ccdB
ccdB death cassette present in 'empty vector' form only (replace with gene of interest)
1876
2085
primer
NAP138
NAP138 reverse sequencing primer 5? TGTTTCGCCATTTATCACCTTC 3?
2405
2384
primer
NAP150
Forward sequencing primer NAP150 5?-CCC ATT GTA TGG GAT CTG ATC-3?
388
408
promoter
T7
T7 transcriptional start sequence
5940
5964
recombination site
attR1
AttR1 site for recombination in empty vector
549
565
recombination site
attR2
AttR2 site for recombination in empty vector
2236
2251
selectable marker
CmR
chloramphenicol resistance
782
1438
selectable marker
AmpR
ampicillin resistance gene
4688
4029
tag
GST
Glutathione transferase (GST) tag
2282
2950
