Gene:
Gene Symbol:
DGKG
Gene Name:
diacylglycerol kinase, gamma 90kDa
Original Clone ID:
FLH231931.01L
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pLDNT7_nFLAG
Format:
CLOSED
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00618004
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 2376nts
Open reading frame: 517 to 2892
CACGGAGATAGACAGCTTTGGAGCTGCTGAACTCCGAGCACAGGGTGAAGACCCCGGCGC
TACCAACCACAGCCTGGCAGCCTGGTCTCCGCGGCACCCACTGGGGCTGCATCCCCCTCC
CCCGAGAGGGCTGCGCAGGCGGGAAGACGCCAGAGGCCAGCTTCGGTCCCCCTTCTGTCT
CTCGGTTCCTCTTTCCTCCCAAGTAAGGGAATAAACCGCGAAGAAGGAGCGCCCCGGGCC
ACCGCGCAACCAAGTGTTGCCTGGTGAGGAAGAGCCAGGACTTCTGAATTTACCTTGAAT
ACAGACAGGAGGATGTTGCCTAAGGAATAGCAGAGATCTTGTCTCATCTTCTGAGAGGTG
CCTGCTGCTGCTGTATACACTTGAGTGCTCCCAGAAGTCTCCTGAAAGGCTTACATCGCA
AACCTGCAATGAGCCAGGCCCTGGGCTGGGCCTCCACTTCAGCCTAGTGAACAAAACTCC
ATCACTGCCCTTTAGCCACTCACATAAAGTTTAAAAATGGGTGAAGAACGGTGGGTCTCC
CTCACTCCAGAAGAATTTGACCAACTCCAGAAATATTCAGAATATTCCTCCAAGAAGATA
AAAGATGCCTTGACTGAATTTAATGAGGGTGGGAGCCTCAAACAATATGACCCACATGAG
CCGATTAGCTATGATGTCTTCAAGCTGTTCATGAGGGCGTACCTGGAGGTGGACCTTCCC
CAGCCACTGAGCACTCACCTCTTCCTGGCCTTCAGCCAGAAGCCCAGACACGAGACCTCT
GACCACCCGACGGAGGGAGCCAGCAACAGTGAGGCCAACAGCGCAGATACTAATATACAG
AATGCAGATAATGCCACCAAAGCAGACGAGGCCTGTGCCCCTGATACTGAATCAAATATG
GCTGAGAAGCAAGCACCAGCTGAAGACCAAGTGGCTGCGACCCCCCTGGAACCCCCCGTC
CCTCGGTCTTCAAGCTCGGAATCCCCAGTGGTGTACCTGAAGGATGTTGTGTGCTACCTG
TCCCTGCTGGAGACGGGGAGGCCTCAGGATAAGCTGGAGTTCATGTTTCGCCTCTATGAT
TCAGATGAGAACGGTCTCCTGGACCAAGCGGAGATGGATTGCATTGTCAACCAAATGCTG
CATATTGCCCAGTACCTGGAGTGGGATCCCACAGAGCTGAGGCCTATATTGAAGGAGATG
CTGCAAGGGATGGACTACGACCGGGACGGCTTTGTGTCTCTACAGGAATGGGTCCATGGA
GGGATGACCACCATCCCATTGCTGGTGCTCCTGGGGATGGATGACTCTGGCTCCAAGGGG
GATGGGGGGCACGCCTGGACCATGAAGCACTTCAAGAAACCAACCTACTGCAACTTCTGC
CATATCATGCTCATGGGCGTCCGCAAGCAAGGCCTGTGCTGCACTTACTGTAAATACACT
GTCCACGAACGCTGTGTGTCCAAAAACATTCCTGGTTGTGTCAAAACGTACTCAAAAGCC
AAAAGGAGTGGTGAGGTGATGCAGCACGCATGGGTGGAAGGGAACTCCTCCGTCAAGTGT
GACCGGTGCCACAAAAGTATCAAGTGCTACCAGAGTGTCACCGCGCGGCACTGCGTGTGG
TGCCGGATGACGTTTCACCGCAAATGTGAATTATCAACGTTGTGTGACGGTGGGGAACTC
AGAGACCACATCTTACTGCCCACCTCCATATGCCCCATCACCCGGGACAGGCCAGGTGAG
AAGTCTGATGGCTGCGTGTCCGCCAAGGGCGAACTTGTCATGCAGTATAAGATCATCCCC
ACCCCGGGTACCCACCCCCTGCTGGTCTTGGTGAACCCCAAGAGTGGAGGGAGACAAGGA
GAAAGAATTCTTCGGAAATTCCACTATCTGCTCAACCCCAAACAAGTTTTCAACCTGGAC
AATGGGGGGCCTACTCCAGGGTTGAACTTTTTCCGTGATACTCCAGACTTCCGTGTTTTG
GCCTGTGGTGGAGATGGGACAGTTGGCTGGATTTTGGATTGCATTGATAAGGCCAACTTT
GCAAAGCATCCACCAGTGGCTGTCCTGCCTCTTGGAACAGGAAATGACCTTGCCCGTTGT
CTCCGCTGGGGAGGAGGTTATGAAGGGGGCAGCTTGACAAAAATCCTGAAAGACATTGAG
CAGAGCCCCTTGGTGATGCTGGACCGCTGGCATCTGGAAGTCATCCCCAGAGAGGAAGTG
GAAAACGGGGACCAGGTCCCATACAGCATCATGAACAACTATTTCTCCATTGGTGTGGAC
GCTTCCATTGCACACAGATTCCATGTGATGAGAGAGAAACATCCTGAAAAATTCAACAGC
AGGATGAAGAACAAGCTGTGGTACTTTGAATTTGGCACCTCGGAGACTTTTGCAGCGACC
TGCAAGAAACTCCACGACCACATTGAGTTGGAGTGTGATGGGGTTGGGGTGGACCTGAGC
AACATCTTCCTGGAAGGCATTGCCATTCTCAACATTCCCAGCATGTACGGAGGCACCAAT
CTCTGGGGAGAAAACAAGAAGAACCGGGCTGTGATCCGGGAAAGCAGGAAGGGTGTCACT
GACCCCAAAGAACTGAAATTCTGCGTTCAAGACCTCAGTGACCAGCTCCTTGAAGTGGTG
GGGCTAGAAGGAGCCATGGAGATGGGGCAGATCTACACCGGCCTGAAGAGTGCAGGCAGG
AGGCTGGCCCAGTGCGCCTCTGTCACCATCAGGACAAACAAGCTGCTGCCAATGCAAGTG
GATGGAGAACCCTGGATGCAGCCATGTTGCACGATTAAAATTACTCACAAGAACCAAGCG
CCCATGATGATGGGGCCTCCCCAGAAGAGCAGCTTCTTCTCGTTGAGAAGGAAGAGCCGT
TCAAAAGACTAAACAGTGTGCCAAACACCAGCTAAACCAAGAGAGAAAGCAAGAAACTAT
AATGCACACTCACACACAATTTATGTGCACACTCACACATGCACACACACACACACATAC
ACACTCTTCTCTAACCAGTGGAAGCAAAGCCACCCTTCGGGAAGAAAACGTCACCTTGCC
ATACATTCTGTTTCAACAGTGGGTACACCCCTAACAGAGCCAGTGCCAACAAAACATTTT
GAATGGACTTAGGGCCCATGAGGTTGTGGCTGGCTTAGGCAGCAACCTCCACATTCCCAC
AGGCCTTGAGCAGAATTTTCTGAGACTGAAGGGAAATCCCCCTTTCTTTCTACCAGCCCT
GCAAGTTTCCTCATGGACGCTCGCGAGGAGCAGGCTGCAGGTTTCCTGCCTATGGTGAGA
TCAGATGTGGCCAAGGGAAGGAGCTCTGGTTCCAGAGAATTTGCACAAAGTTCCCTCTGT
ACAGAGACAAAACGGCCTCCGGCTCTCAGAGCATAATCCTTGGCAGGGCTCAGCAGGCGC
ACGTTGGTTTCTTGGTCGTCCTTTGAGTGACAACTTCTCCGTGAACCTGCTGAAGAGGCA
GAAAGGCTGTGGAAAGCTGTATTTCCATTCTTGGGTTTCTGCGCCGTCGGTGGGCACTTG
TTATTTTCCAGGAACCTTCTCCTGGTGTCTACATGTTTGCTTAGAGGCGGCTCCAAGAGC
CCCAGAGCTGCCTGCATAGCACACCTTAGATGTGGTATTTATTTTCTTAGTTCTGTGAAC
ACCTGGGAGGGAGAGCGGAGAAACTGGGATTTATTTTTCAAATTGGTGTCATAATATTGT
GTAAAAAGGGAAGGAAAAAAAAAACCACCCCCAGCTTC
Protein sequence predicted based on nucleotide sequence
MGEERWVSLTPEEFDQLQKYSEYSSKKIKDALTEFNEGGSLKQYDPHEPISYDVFKLFMR AYLEVDLPQPLSTHLFLAFSQKPRHETSDHPTEGASNSEANSADTNIQNADNATKADEAC APDTESNMAEKQAPAEDQVAATPLEPPVPRSSSSESPVVYLKDVVCYLSLLETGRPQDKL EFMFRLYDSDENGLLDQAEMDCIVNQMLHIAQYLEWDPTELRPILKEMLQGMDYDRDGFV SLQEWVHGGMTTIPLLVLLGMDDSGSKGDGGHAWTMKHFKKPTYCNFCHIMLMGVRKQGL CCTYCKYTVHERCVSKNIPGCVKTYSKAKRSGEVMQHAWVEGNSSVKCDRCHKSIKCYQS VTARHCVWCRMTFHRKCELSTLCDGGELRDHILLPTSICPITRDRPGEKSDGCVSAKGEL VMQYKIIPTPGTHPLLVLVNPKSGGRQGERILRKFHYLLNPKQVFNLDNGGPTPGLNFFR DTPDFRVLACGGDGTVGWILDCIDKANFAKHPPVAVLPLGTGNDLARCLRWGGGYEGGSL TKILKDIEQSPLVMLDRWHLEVIPREEVENGDQVPYSIMNNYFSIGVDASIAHRFHVMRE KHPEKFNSRMKNKLWYFEFGTSETFAATCKKLHDHIELECDGVGVDLSNIFLEGIAILNI PSMYGGTNLWGENKKNRAVIRESRKGVTDPKELKFCVQDLSDQLLEVVGLEGAMEMGQIY TGLKSAGRRLAQCASVTIRTNKLLPMQVDGEPWMQPCCTIKITHKNQAPMMMGPPQKSSF FSLRRKSRSKD*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Host Type: bacterial Marker: chloramphenicol
Host Type: bacterial Marker: chloramphenicol
Bacterial Selection Condition:
100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition:
Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments:
Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pLDNT7_nFLAG
Synonyms:
pLDNT7-Flag
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Vector for in vitro translation with mammalian extracts, adds N-terminal Flag epitope tag; amp resistance; recombinational cloning
Comments:
None
Size (bp):
4557
Parent Vector:
None
Properties:
Creator, acceptor (destination), in vitro transcription, loxP, ssDNA production, with tag/fusion/marker
Author Name:
Daniel Schiwek
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE1 ori
ColE1 origin of replication
1842
2524
promoter
T7
T7 promoter
4462
4479
recombination site
LoxP
LoxP site for recombinational cloning
657
690
selectable marker
AmpR
ampicillin resistance gene
2622
3281
ssDNA origin
f1 ori
f1 origin for ssDNA production
3928
4368
tag
Flag
3xFlag epitope tag ORF and LoxP region
585
650
trxn termination sequence
T7 term
T7 terminator sequence
1614
1660
