Gene:
Gene Symbol:
MAP4K2
Gene Name:
mitogen-activated protein kinase kinase kinase kinase 2
Original Clone ID:
HsCD00298686
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pLDNT7_nFLAG
Format:
CLOSED
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00618045
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 2463nts
Open reading frame: 93 to 2555
CAGAGCCACGGGCGCCCGCCCCGCCCCGCGCCGCCCCGCGCCGGCTCCGCAGCTCGCGCC
CGCCCGCCTGCCGGCCCGCCCGGCGCCGGGCCATGGCGCTGCTGCGGGATGTGTCGCTGC
AGGACCCGCGGGACCGCTTCGAGCTGCTGCAGCGCGTGGGGGCCGGGACCTATGGCGACG
TCTACAAGGCCCGCGACACGGTCACGTCCGAACTGGCCGCCGTGAAGATAGTCAAGCTAG
ACCCAGGGGACGACATCAGCTCCCTCCAGCAGGAAATCACCATCCTGCGTGAGTGCCGCC
ACCCCAATGTGGTGGCCTACATTGGCAGCTACCTCAGGAATGACCGCTTGTGGATCTGCA
TGGAGTTCTGCGGAGGGGGCTCCCTGCAGGAGATTTACCATGCCACTGGGCCCCTGGAGG
AGCGGCAGATTGCCTACGTCTGCCGAGAGGCACTGAAGGGGCTCCACCACCTGCATTCTC
AGGGGAAGATCCACAGAGACATCAAGGGAGCCAACCTTCTCCTCACTCTCCAGGGAGATG
TCAAACTGGCTGACTTTGGGGTGTCAGGCGAGCTGACAGCGTCTGTGGCCAAGAGGAGGT
CTTTCATTGGGACTCCCTACTGGATGGCTCCCGAGGTGGCTGCTGTGGAGCGCAAAGGTG
GCTACAATGAGCTATGTGACGTCTGGGCCCTGGGCATCACTGCCATTGAGCTGGGCGAGC
TGCAGCCCCCTCTGTTCCACCTGCACCCCATGAGGGCCCTGATGCTCATGTCGAAGAGCA
GCTTCCAGCCGCCCAAACTGAGAGATAAGACTCGCTGGACCCAGAATTTCCACCACTTTC
TCAAACTGGCCCTGACCAAGAATCCTAAGAAGAGGCCGACAGCAGAGAAGCTCCTGCAGC
ACCCGTTCACGACTCAGCAGCTCCCTCGGGCCCTCCTCACACAGCTGCTGGACAAAGCCA
GTGACCCTCATCTGGGGACCCCCTCCCCTGAGGACTGTGAGCTGGAGACCTATGACATGT
TTCCAGACACCATTCACTCCCGGGGGCAGCACGGCCCAGCCGAGAGGACCCCCTCGGAGA
TCCAGTTTCACCAGGTGAAATTTGGCGCCCCACGCAGGAAGGAAACTGACCCACTGAATG
AGCCGTGGGAGGAAGAGTGGACACTACTGGGAAAGGAAGAGTTGAGTGGGAGCCTGCTGC
AGTCGGTCCAGGAGGCCCTGGAGGAAAGGAGTCTGACTATTCGGTCAGCCTCAGAATTCC
AGGAGCTGGACTCCCCAGACGATACCATGGGAACCATCAAGCGGGCCCCGTTCCTAGGGC
CACTCCCCACTGACCCTCCAGCAGAGGAGCCTCTGTCCAGTCCCCCAGGAACCCTGCCCC
CACCTCCTTCAGGCCCCAACAGCTCCCCACTGCTGCCCACGGCCTGGGCCACCATGAAGC
AGCGGGAGGATCCTGAGAGGTCATCCTGCCACGGGCTCCCCCCAACTCCCAAGGTGCATA
TGGGCGCCTGCTTCTCCAAGGTCTTCAATGGCTGCCCCCTGCGGATCCACGCTGCTGTCA
CCTGGATTCACCCTGTTACTCGGGACCAGTTCCTGGTGGTAGGGGCCGAGGAAGGCATCT
ACACACTCAACCTGCATGAACTGCATGAGGATACGCTGGAGAAGCTGATTTCACATCGCT
GCTCCTGGCTCTACTGCGTGAACAACGTGCTGCTGTCACTCTCAGGGAAATCCACGCACA
TCTGGGCCCATGACCTCCCAGGCCTGTTTGAGCAGCGGAGGCTACAGCAACAGGTTCCCC
TCTCCATCCCCACCAACCGCCTCACCCAGCGCATCATCCCCAGGCGCTTTGCTCTGTCCA
CCAAGATTCCTGACACCAAAGGCTGCTTGCAGTGTCGTGTGGTGCGGAACCCCTACACGG
GTGCCACCTTCCTGCTGGCCGCCCTGCCCACCAGCCTGCTCCTGCTGCAGTGGTATGAGC
CGCTGCAGAAGTTTCTGCTGCTGAAGAACTTCTCCAGCCCTCTGCCCAGCCCAGCTGGGA
TGCTGGAGCCGCTGGTGCTGGATGGGAAGGAGCTGCCGCAGGTGTGTGTTGGGGCCGAGG
GGCCTGAGGGGCCCGGCTGCCGCGTCCTGTTCCATGTCCTGCCCCTGGAGGCTGGCCTGA
CGCCCGACATCCTCATCCCACCTGAGGGGATCCCAGGCTCGGCCCAGCAGGTGATCCAGG
TGGACAGGGACACAATCCTAGTCAGCTTTGAACGCTGTGTGAGGATTGTCAACATGCAGG
GCGAGCCCACGGCCACACTGGCACCTGAGCTGACCTTTGATTTCCCCATCGAGACTGTGG
TGTGCCTGCAGGACAGTGTGCTGGCCTTCTGGAGCCATGGGATGCAAGGCCGAAGCCTGG
ATACCAATGAGGTGACCCAGGAGATCACAGATGAAACAAGGATCTTCCGAGTGCTTGGGG
CCCACAGAGACATCATCCTGGAGAGCATTCCCACTGACAACCCAGAGGCGCACAGCAACC
TCTACATCCTCACGGGCCACCAGAGCACCTACTAAGAGCAGCGGGCCTGTCCAGGGGCTC
CCCGCCCCACCCCACGCCTTAGCTGCAGGCCCTTTTGGGCAAAGGGGCCCATCCTAGACC
AGAGGAGCCCAGGCCCTGGCCCTGCTGGGGCTGAAGGTCAGAAGTAATCCTGAGAAATGT
TTCAGGCCTGGGGAGGGAGGGGAGCCCCCGACGCCTCTGCAATAACTGGACCAGGGGGAG
CTGCTGTCACTCCCCCATCCCCGAGGCAGCCCAGTCCCTAGTGCCCAAGGCAGGGACCCT
GGGCCTGGGCCATCCATTCCATTTTGTTCCACATTTCCTTTCTACTCTTTCTGCCAAGAG
CCTGCCCCTGCATTTGTCCTGGGAAACACGGTATTTAAGAGAGAACTATATTGGTATTAA
AGCTGGTTTGTTTTAAAAAAAAAA
Protein sequence predicted based on nucleotide sequence
MALLRDVSLQDPRDRFELLQRVGAGTYGDVYKARDTVTSELAAVKIVKLDPGDDISSLQQ EITILRECRHPNVVAYIGSYLRNDRLWICMEFCGGGSLQEIYHATGPLEERQIAYVCREA LKGLHHLHSQGKIHRDIKGANLLLTLQGDVKLADFGVSGELTASVAKRRSFIGTPYWMAP EVAAVERKGGYNELCDVWALGITAIELGELQPPLFHLHPMRALMLMSKSSFQPPKLRDKT RWTQNFHHFLKLALTKNPKKRPTAEKLLQHPFTTQQLPRALLTQLLDKASDPHLGTPSPE DCELETYDMFPDTIHSRGQHGPAERTPSEIQFHQVKFGAPRRKETDPLNEPWEEEWTLLG KEELSGSLLQSVQEALEERSLTIRSASEFQELDSPDDTMGTIKRAPFLGPLPTDPPAEEP LSSPPGTLPPPPSGPNSSPLLPTAWATMKQREDPERSSCHGLPPTPKVHMGACFSKVFNG CPLRIHAAVTWIHPVTRDQFLVVGAEEGIYTLNLHELHEDTLEKLISHRCSWLYCVNNVL LSLSGKSTHIWAHDLPGLFEQRRLQQQVPLSIPTNRLTQRIIPRRFALSTKIPDTKGCLQ CRVVRNPYTGATFLLAALPTSLLLLQWYEPLQKFLLLKNFSSPLPSPAGMLEPLVLDGKE LPQVCVGAEGPEGPGCRVLFHVLPLEAGLTPDILIPPEGIPGSAQQVIQVDRDTILVSFE RCVRIVNMQGEPTATLAPELTFDFPIETVVCLQDSVLAFWSHGMQGRSLDTNEVTQEITD ETRIFRVLGAHRDIILESIPTDNPEAHSNLYILTGHQSTY*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Host Type: bacterial Marker: chloramphenicol
Host Type: bacterial Marker: chloramphenicol
Bacterial Selection Condition:
100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition:
Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments:
Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pLDNT7_nFLAG
Synonyms:
pLDNT7-Flag
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Vector for in vitro translation with mammalian extracts, adds N-terminal Flag epitope tag; amp resistance; recombinational cloning
Comments:
None
Size (bp):
4557
Parent Vector:
None
Properties:
Creator, acceptor (destination), in vitro transcription, loxP, ssDNA production, with tag/fusion/marker
Author Name:
Daniel Schiwek
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE1 ori
ColE1 origin of replication
1842
2524
promoter
T7
T7 promoter
4462
4479
recombination site
LoxP
LoxP site for recombinational cloning
657
690
selectable marker
AmpR
ampicillin resistance gene
2622
3281
ssDNA origin
f1 ori
f1 origin for ssDNA production
3928
4368
tag
Flag
3xFlag epitope tag ORF and LoxP region
585
650
trxn termination sequence
T7 term
T7 terminator sequence
1614
1660
