Gene:
Gene Symbol:
TRIM24
Gene Name:
tripartite motif containing 24
Original Clone ID:
FLH230839.01X
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pLDNT7_nFLAG
Format:
CLOSED
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00618047
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 3153nts
Open reading frame: 114 to 3266
AGCGGGTCCACCGAGCGGCCTCTGAGGAGCAGCCGCAGGAGGAGGAGGAGGTCGTCGGGG
GCGGCGGGCGGAGACCGCGCTCTCGCTTCCCCGGCGGCGGCAAGGGCAGGACAATGGAGG
TGGCGGTGGAGAAGGCGGTGGCGGCGGCGGCAGCGGCCTCGGCTGCGGCCTCCGGGGGGC
CCTCGGCGGCGCCGAGCGGGGAGAACGAGGCCGAGAGTCGGCAGGGCCCGGACTCGGAGC
GCGGCGGCGAGGCGGCCCGGCTCAACCTGTTGGACACTTGCGCCGTGTGCCACCAGAACA
TCCAGAGCCGGGCGCCCAAGCTGCTGCCCTGCCTGCACTCTTTCTGCCAGCGCTGCCTGC
CCGCGCCCCAGCGCTACCTCATGCTGCCCGCGCCCATGCTGGGCTCGGCCGAGACCCCGC
CACCCGTCCCTGCCCCCGGCTCGCCGGTCAGCGGCTCGTCGCCGTTCGCCACCCAAGTTG
GAGTCATTCGTTGCCCAGTTTGCAGCCAAGAATGTGCAGAGAGACACATCATAGATAACT
TTTTTGTGAAGGACACTACTGAGGTTCCCAGCAGTACAGTAGAAAAGTCAAATCAGGTAT
GTACAAGCTGTGAGGACAACGCAGAAGCCAATGGGTTTTGTGTAGAGTGTGTTGAATGGC
TCTGCAAGACGTGTATCAGAGCTCATCAGAGGGTAAAGTTCACAAAAGACCACACTGTCA
GACAGAAAGAGGAAGTATCTCCAGAGGCAGTTGGTGTCACCAGCCAGCGACCAGTGTTTT
GTCCTTTTCATAAAAAGGAGCAGCTGAAGCTGTACTGTGAGACATGTGACAAACTGACAT
GTCGAGACTGTCAGTTGTTAGAACATAAAGAGCATAGATACCAATTTATAGAAGAAGCTT
TTCAGAATCAGAAAGTGATCATAGATACACTAATCACCAAACTGATGGAAAAAACAAAAT
ACATAAAATTCACAGGAAATCAGATCCAAAACAGAATTATTGAAGTAAATCAAAATCAAA
AGCAGGTGGAACAGGATATTAAAGTTGCTATATTTACACTGATGGTAGAAATAAATAAAA
AAGGAAAAGCTCTACTGCATCAGTTAGAGAGCCTTGCAAAGGACCATCGCATGAAACTTA
TGCAACAACAACAGGAAGTGGCTGGACTCTCTAAACAATTGGAGCATGTCATGCATTTTT
CTAAATGGGCAGTTTCCAGTGGCAGCAGTACAGCATTACTTTATAGCAAACGACTGATTA
CATACCGGTTACGACACCTCCTTCGTGCAAGGTGTGATGCATCCCCAGTGACCAACAACA
CCATCCAATTTCACTGTGATCCTAGTTTCTGGGCTCAAAATATCATCAACTTAGGTTCTT
TAGTAATCGAGGATAAAGAGAGCCAGCCACAAATGCCTAAGCAGAATCCTGTCGTGGAAC
AGAATTCACAGCCACCAAGTGGTTTATCATCAAACCAGTTATCCAAGTTCCCAACACAGA
TCAGCCTAGCTCAATTACGGCTCCAGCATATGCAGCAACAGGTAATGGCTCAGAGGCAAC
AGGTGCAACGGAGGCCAGCACCTGTGGGTTTACCAAACCCTAGAATGCAGGGGCCCATCC
AGCAACCTTCCATCTCTCATCAGCAACCGCCTCCACGTTTGATAAACTTTCAGAATCACA
GCCCCAAACCCAATGGACCAGTTCTTCCTCCTCATCCTCAACAACTGAGATATCCACCAA
ACCAGAACATACCACGACAAGCAATAAAGCCAAACCCCCTACAGATGGCTTTCTTGGCTC
AACAAGCCATAAAACAGTGGCAGATCAGCAGTGGACAGGGAACCCCATCAACTACCAACA
GCACATCCTCTACTCCTTCCAGCCCCACGATTACTAGTGCAGCAGGATATGATGGAAAGG
CTTTTGGTTCACCTATGATCGATTTGAGCTCACCAGTGGGAGGGTCTTATAATCTTCCCT
CTCTTCCGGATATTGACTGTTCAAGTACTATTATGCTGGACAATATTGTGAGGAAAGATA
CTAATATAGATCATGGCCAGCCAAGACCACCCTCAAACAGAACGGTCCAGTCACCAAATT
CATCAGTGCCATCTCCAGGCCTTGCAGGACCTGTTACTATGACTAGTGTACACCCCCCAA
TACGTTCACCTAGTGCCTCCAGCGTTGGAAGCCGAGGAAGCTCTGGCTCTTCCAGCAAAC
CAGCAGGAGCTGACTCTACACACAAAGTCCCAGTGGTCATGCTGGAGCCAATTCGAATAA
AACAAGAAAACAGTGGACCACCGGAAAATTATGATTTCCCTGTTGTTATAGTGAAGCAAG
AATCAGATGAAGAATCTAGGCCTCAAAATGCCAATTATCCAAGAAGCATACTCACCTCCC
TGCTCTTAAATAGCAGTCAGAGCTCTACTTCTGAGGAGACTGTGCTAAGATCAGATGCCC
CTGATAGTACAGGAGATCAACCTGGACTTCACCAGGACAATTCCTCAAATGGAAAGTCTG
AATGGTTGGATCCTTCCCAGAAGTCACCTCTTCATGTTGGAGAGACAAGGAAAGAGGATG
ACCCCAATGAGGACTGGTGTGCAGTTTGTCAAAACGGAGGGGAACTCCTCTGCTGTGAAA
AGTGCCCCAAAGTATTCCATCTTTCTTGTCATGTGCCCACATTGACAAATTTTCCAAGTG
GAGAGTGGATTTGCACTTTCTGCCGAGACTTATCTAAACCAGAAGTTGAATATGATTGTG
ATGCTCCCAGTCACAACTCAGAAAAAAAGAAAACTGAAGGCCTTGTTAAGTTAACACCTA
TAGATAAAAGGAAGTGTGAGCGCCTACTTTTATTTCTTTACTGCCATGAAATGAGCCTGG
CTTTTCAAGACCCTGTTCCTCTAACTGTGCCTGATTATTACAAAATAATTAAAAATCCAA
TGGATTTGTCAACCATCAAGAAAAGACTACAAGAAGATTATTCCATGTACTCAAAACCTG
AAGATTTTGTAGCTGATTTTAGATTGATCTTTCAAAACTGTGCTGAATTCAATGAGCCTG
ATTCAGAAGTAGCCAATGCTGGTATAAAACTTGAAAATTATTTTGAAGAACTTCTAAAGA
ACCTCTATCCAGAAAAAAGGTTTCCCAAACCAGAATTCAGGAATGAATCAGAAGATAATA
AATTTAGTGATGATTCAGATGATGACTTTGTACAGCCCCGGAAGAAACGCCTCAAAAGCA
TTGAAGAACGCCAGTTGCTTAAATAATATGCAGCACCACTAGCTTGTGCTGGTTTTTAGA
TTTTTTTGTTTTCAAAAAAACATTTGTCAGTAATTTAACATCACTACAAAAAGAAGAGTT
TGTGACTATTCTCATCTCTGTTTTGGACGTTTACTAGACTTTGATTTCCTTAATAGCCCA
TTTCTGTTAACCTCTTATCACTAAGAAAGAAAGGAAAGAAGGAGATGAATAGAAGAAAGA
AAATGGAAAGAAGGAAAAAAGGAGGATAGAAAAAGGATGGAAGAAAGAAGCATTGAAAAC
AAAGACATTCTTCCCACTTCTTGGATTTTTAAACCACAGTCTGGAGTGATAGCTACTGTA
GAAAGGAAATAGACTTTGTATGAACTCTTTAAGTTGAAAAGTAAAAAATATATGTGGTTT
GGATGTGTGCTTTAATTCAGCTTTAGAAATTAATACCACTACCCGTGAATTATATGGCCT
GACAATATGAATTAGGTGTACTGTACTGAAGAACAGTACTCCACAAACATGGGTGGTAAC
AAGAGTTCCATCCCAGGAGGCCAAACGGTGCAACAGAAGGGTAGGTTAGATGCTATTAAG
AAGGCACTTAATAGTACATCATGTAAGATGGCAACTGTATTAAAGAAAAATCCGAAAAAA
AAAAAAAAA
Protein sequence predicted based on nucleotide sequence
MEVAVEKAVAAAAAASAAASGGPSAAPSGENEAESRQGPDSERGGEAARLNLLDTCAVCH QNIQSRAPKLLPCLHSFCQRCLPAPQRYLMLPAPMLGSAETPPPVPAPGSPVSGSSPFAT QVGVIRCPVCSQECAERHIIDNFFVKDTTEVPSSTVEKSNQVCTSCEDNAEANGFCVECV EWLCKTCIRAHQRVKFTKDHTVRQKEEVSPEAVGVTSQRPVFCPFHKKEQLKLYCETCDK LTCRDCQLLEHKEHRYQFIEEAFQNQKVIIDTLITKLMEKTKYIKFTGNQIQNRIIEVNQ NQKQVEQDIKVAIFTLMVEINKKGKALLHQLESLAKDHRMKLMQQQQEVAGLSKQLEHVM HFSKWAVSSGSSTALLYSKRLITYRLRHLLRARCDASPVTNNTIQFHCDPSFWAQNIINL GSLVIEDKESQPQMPKQNPVVEQNSQPPSGLSSNQLSKFPTQISLAQLRLQHMQQQVMAQ RQQVQRRPAPVGLPNPRMQGPIQQPSISHQQPPPRLINFQNHSPKPNGPVLPPHPQQLRY PPNQNIPRQAIKPNPLQMAFLAQQAIKQWQISSGQGTPSTTNSTSSTPSSPTITSAAGYD GKAFGSPMIDLSSPVGGSYNLPSLPDIDCSSTIMLDNIVRKDTNIDHGQPRPPSNRTVQS PNSSVPSPGLAGPVTMTSVHPPIRSPSASSVGSRGSSGSSSKPAGADSTHKVPVVMLEPI RIKQENSGPPENYDFPVVIVKQESDEESRPQNANYPRSILTSLLLNSSQSSTSEETVLRS DAPDSTGDQPGLHQDNSSNGKSEWLDPSQKSPLHVGETRKEDDPNEDWCAVCQNGGELLC CEKCPKVFHLSCHVPTLTNFPSGEWICTFCRDLSKPEVEYDCDAPSHNSEKKKTEGLVKL TPIDKRKCERLLLFLYCHEMSLAFQDPVPLTVPDYYKIIKNPMDLSTIKKRLQEDYSMYS KPEDFVADFRLIFQNCAEFNEPDSEVANAGIKLENYFEELLKNLYPEKRFPKPEFRNESE DNKFSDDSDDDFVQPRKKRLKSIEERQLLK*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Host Type: bacterial Marker: chloramphenicol
Host Type: bacterial Marker: chloramphenicol
Bacterial Selection Condition:
100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition:
Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments:
Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pLDNT7_nFLAG
Synonyms:
pLDNT7-Flag
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Vector for in vitro translation with mammalian extracts, adds N-terminal Flag epitope tag; amp resistance; recombinational cloning
Comments:
None
Size (bp):
4557
Parent Vector:
None
Properties:
Creator, acceptor (destination), in vitro transcription, loxP, ssDNA production, with tag/fusion/marker
Author Name:
Daniel Schiwek
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE1 ori
ColE1 origin of replication
1842
2524
promoter
T7
T7 promoter
4462
4479
recombination site
LoxP
LoxP site for recombinational cloning
657
690
selectable marker
AmpR
ampicillin resistance gene
2622
3281
ssDNA origin
f1 ori
f1 origin for ssDNA production
3928
4368
tag
Flag
3xFlag epitope tag ORF and LoxP region
585
650
trxn termination sequence
T7 term
T7 terminator sequence
1614
1660
