DNASU Plasmid | ATP6V1A (H. sapiens )<br>In pLDNT7_nFLAG (Flag-tagged<br>In vitro expression vector)

Explanation of Terms

Gene:

Gene Symbol: PKP2

Gene Name: plakophilin 2

Original Clone ID:

FLH232116.01L

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pLDNT7_nFLAG

Format:

CLOSED

Source:

Center for Personalized Diagnostics

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Center for Personalized Diagnostics

HsCD00618128

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1854nts

Open reading frame: 57 to 1910

Reference Sequence Alignment

GGGTCCTCGGTCGGTACAGTCTCTGCACCTCGCGCCCCAGCAGGTAAACTAACATTATGG
ATTTTTCCAAGCTACCCAAAATACTCGATGAAGATAAAGAAAGCACATTTGGTTATGTGC
ATGGGGTCTCAGGACCTGTGGTTACAGCCTGTGACATGGCGGGTGCAGCCATGTATGAGC
TGGTGAGAGTGGGCCACAGCGAATTGGTTGGAGAGATTATTCGATTGGAGGGTGACATGG
CTACTATTCAGGTGTATGAAGAAACTTCTGGTGTGTCTGTTGGAGATCCTGTACTTCGCA
CTGGTAAACCCCTCTCTGTAGAGCTTGGTCCTGGCATTATGGGAGCCATTTTTGATGGTA
TTCAAAGACCTTTGTCGGATATCAGCAGTCAGACCCAAAGCATCTACATCCCCAGAGGAG
TAAACGTGTCTGCTCTTAGCAGAGATATCAAATGGGACTTTACACCTTGCAAAAACCTAC
GGGTTGGTAGTCATATCACTGGCGGAGACATTTATGGAATTGTCAGTGAGAACTCGCTTA
TCAAACACAAAATCATGTTACCCCCACGAAACAGAGGAACTGTAACTTACATTGCTCCAC
CTGGGAATTATGATACCTCTGATGTTGTCTTGGAGCTTGAATTTGAAGGTGTAAAGGAGA
AGTTCACCATGGTGCAAGTATGGCCTGTACGTCAAGTTCGACCTGTCACTGAGAAGCTGC
CAGCCAATCATCCTCTGTTGACTGGCCAGAGAGTCCTTGATGCCCTTTTTCCGTGTGTCC
AGGGAGGAACTACTGCTATCCCTGGAGCCTTTGGCTGTGGAAAGACAGTGATATCACAGT
CTCTATCCAAGTATTCTAACAGTGATGTAATCATCTATGTAGGATGTGGTGAAAGAGGAA
ATGAGATGTCTGAAGTCCTCCGGGACTTCCCAGAGCTCACAATGGAGGTTGATGGTAAGG
TAGAGTCAATTATGAAGAGGACAGCTTTGGTAGCCAATACCTCCAATATGCCTGTTGCTG
CTAGAGAAGCCTCTATTTATACTGGAATCACACTGTCAGAGTACTTCCGTGACATGGGCT
ATCATGTCAGTATGATGGCTGACTCTACCTCTAGATGGGCTGAGGCCCTTAGAGAAATCT
CTGGTCGTTTAGCTGAAATGCCTGCAGATAGTGGATATCCAGCCTATCTTGGTGCCCGTC
TGGCCTCGTTTTATGAACGAGCAGGCAGGGTGAAATGTCTTGGAAATCCTGAAAGAGAAG
GGAGTGTCAGCATTGTAGGAGCAGTTTCTCCACCTGGTGGTGATTTTTCTGATCCAGTTA
CATCTGCCACTCTTGGTATCGTTCAGGTGTTCTGGGGCTTAGATAAGAAACTAGCTCAAC
GTAAGCATTTCCCCTCTGTCAATTGGCTCATCAGCTACAGCAAGTATATGCGTGCCTTGG
ATGAATACTATGACAAACACTTCACAGAGTTCGTTCCTCTGAGGACGAAAGCTAAGGAAA
TTCTGCAGGAAGAAGAAGACCTGGCAGAAATTGTACAGCTTGTGGGAAAGGCTTCTTTGG
CAGAAACAGATAAAATCACTCTGGAGGTAGCAAAACTTATCAAAGATGATTTCCTACAAC
AAAATGGATATACTCCTTATGACAGGTTCTGCCCATTCTACAAGACAGTAGGGATGCTGT
CCAACATGATTGCATTTTATGATATGGCTCGTAGAGCTGTTGAAACCACTGCCCAGAGTG
ACAATAAAATCACATGGTCCATTATTCGTGAGCACATGGGAGACATCCTCTATAAACTTT
CCTCCATGAAATTCAAGGATCCACTGAAAGATGGTGAGGCAAAGATCAAAAGCGACTATG
CACAACTTCTTGAAGACATGCAGAATGCATTCCGTAGCCTTGAAGATTAGAAGCCTTGAA
GATTACAACTGTGATTTCCTTTTCCTCAGCAAGCTCCTATGTGTATATTTTCCTGAATTT
CTCATCTCAAACCCTTTGCTTCTTTATTGTGCAGCTTTGAGACTAGTGCCTATGTGTGTT
ATTTGTTTCCCTGTTTTTTTGGTAGGTCTTATATAAAACAAACATTCCTTTGTTCTAGTG
TTGTGAAGGGCCTCCCTCTTCCTTTATCTGAAGTGGTGAATATAGTAAATATACATTCTG
GTTACACTACTGTAAACTTGTATGTAGGGTGATGACCCTCTTTGTCCTAGGTGTACCCTT
TCCTCATCTCTATTAAATTGTAAACAGGACTACTGCATGTACTCTCTTTGCAGTGAATTT
GGAATGGAAGGCCAGGTTTCTATAACTTTTGAACAGGTACTTTGTGCAATGACTCAATTT
CTATTGTGGTAAGCTCATTGGCAGCTTAGCATTTTGCAAAGGAATTGCTTTGCAGGAAAT
ATTTAATTTTCAAAAACATAATGATTAATGTTCCAATTATGCAACACTTCCCCCAGTATA
AATCAGGAATGTTTGTGAGAAACCATTGGGAACTATACTCTTTTTATTTTTATTTTTTAT
TTTTTTTATTATTTTTTTTTTGGGGACGGAGTGTCCCTCTTGTTGCCCAGGCTGGAGTGC
AATGGCGTGATCTTGGCTCACTGCAGCCTTCGCCTCCCGGGTTCAAGTGATTCTCCTGCC
TCAGCCTCCCGAGTAGCTGGGATTACAGGCATGCTCCACCATGCCCAGCTAATTTTGTAT
TTTTAGTAGAAACGGGGTTTCACCATATTGGTCAGGCTGGTCTCGAACTCCAGACCTCAG
GTGATCCGCCCACCTCGGCCTCCCAAACTGCTGGGATTACAGGCGTGAGCCACCGCGCCT
GGCCAGGGACTATACTCTTTTTAAAATAGACATTTGTGGGGCTCACACAATATATGAAAT
AGTACCCTCTAAAAAAAAAAAAAAAAA

Protein sequence predicted based on nucleotide sequence

MDFSKLPKILDEDKESTFGYVHGVSGPVVTACDMAGAAMYELVRVGHSELVGEIIRLEGD
MATIQVYEETSGVSVGDPVLRTGKPLSVELGPGIMGAIFDGIQRPLSDISSQTQSIYIPR
GVNVSALSRDIKWDFTPCKNLRVGSHITGGDIYGIVSENSLIKHKIMLPPRNRGTVTYIA
PPGNYDTSDVVLELEFEGVKEKFTMVQVWPVRQVRPVTEKLPANHPLLTGQRVLDALFPC
VQGGTTAIPGAFGCGKTVISQSLSKYSNSDVIIYVGCGERGNEMSEVLRDFPELTMEVDG
KVESIMKRTALVANTSNMPVAAREASIYTGITLSEYFRDMGYHVSMMADSTSRWAEALRE
ISGRLAEMPADSGYPAYLGARLASFYERAGRVKCLGNPEREGSVSIVGAVSPPGGDFSDP
VTSATLGIVQVFWGLDKKLAQRKHFPSVNWLISYSKYMRALDEYYDKHFTEFVPLRTKAK
EILQEEEDLAEIVQLVGKASLAETDKITLEVAKLIKDDFLQQNGYTPYDRFCPFYKTVGM
LSNMIAFYDMARRAVETTAQSDNKITWSIIREHMGDILYKLSSMKFKDPLKDGEAKIKSD
YAQLLEDMQNAFRSLED*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin
Host Type: bacterial Marker: chloramphenicol

Bacterial Selection Condition: 100 ug/mL ampicillin, 34 ug/mL chloramphenicol

Growth Condition: Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.

Comments: Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pLDNT7_nFLAG

Synonyms:

pLDNT7-Flag

Sequencing Primer:

Forward: T7

Reverse: T7 terminator

Description:

Vector for in vitro translation with mammalian extracts, adds N-terminal Flag epitope tag; amp resistance; recombinational cloning

Comments:

None

Size (bp):

4557

Parent Vector:

None

Properties:

Creator, acceptor (destination), in vitro transcription, loxP, ssDNA production, with tag/fusion/marker

Author Name:

Daniel Schiwek

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

GGGCGAATTAATTCCGGTTATTTTCCACCATATTGCCGTCTTTTGGCAATGTGAGGGCCCGGAAACCTGGCCCTGTCTTCTTGACGAGCATTCCTAGGGGTCTTTCCCCTCTCGCCAAAGGAATGCAAGGTCTGTTGAATGTCGTGAAGGAAGCAGTTCCTCTGGAAGCTTCTTGAAGACAAACAACGTCTGTAGCGACCCTTTGCAGGCAGCGGAACCCCCCACCTGGCGACAGGTGCCTCTGCGGCCAAAAGCCACGTGTATAAGATACACCTGCAAAGGCGGCACAACCCCAGTGCCACGTTGTGAGTTGGATAGTTGTGGAAAGAGTCAAATGGCTCACCTCAAGCGTATTCAACAAGGGGCTGAAGGATGCCCAGAAGGTACCCCATTGTATGGGATCTGATCTGGGGCCTCGGTGCACATGCTTTACATGTGTTTAGTCGAGGTTAAAAAACGTCTAGGCCCCCCGAACCACGGGGACGTGGTTTTCCTTTGAAAAACACGATGATAATATGGATCGGATCCGAATTCGAGCTCCGTCATCAACAAGTTTGTACAAAAAAGCAGGCTCCACCATGGCCGACTACAAGGACCACGACGGCGACTACAAGGACCACGACATCGACTACAAGGACGACGACGACAAGAGATCTATAACTTCGTATAGCATACATTATACGAAGTTATTGATCCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTTTGGACCCAGCTTTCTTGTACAAAGTGGTTGATGACAAGCTTGCGGCCGCACTCGAGCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCACTCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATGAGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTGGAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACAGTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTGGTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTGGATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAACTCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCGAAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCATCCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCCAATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTGAAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCATGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCCAAAATCGGATCTGGTTCCGCGTTGAAGATCTGACTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTTTAAACACTAGTCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAAGGAGGAACTATATCCGGGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGAAATTGTAAACGTTAATATTTTGTTAAAATTCGCGTTAAATTTTTGTTAAATCAGCTCATTTTTTAACCAATAGGCCGAAATCGGCAAAATCCCTTATAAATCAAAAGAATAGACCGAGATAGGGTTGAGTGTTGTTCCAGTTTGGAACAAGAGTCCACTATTAAAGAACGTGGACTCCAACGTCAAAGGGCGAAAAACCGTCTATCAGGGCGATGGCCCACTACGTGAACCATCACCCTAATCAAGTTTTTTGGGGTCGAGGTGCCGTAAAGCACTAAATCGGAACCCTAAAGGGAGCCCCCGATTTAGAGCTTGACGGGGAAAGCCGGCGAACGTGGCGAGAAAGGAAGGGAAGAAAGCGAAAGGAGCGGGCGCTAGGGCGCTGGCAAGTGTAGCGGTCACGCTGCGCGTAACCACCACACCCGCCGCGCTTAATGCGCCGCTACAGGGCGCGTCGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATTGTAATACGACTCACTATA

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ColE1 ori

ColE1 origin of replication

1842

2524

promoter

T7 promoter

4462

4479

recombination site

LoxP

LoxP site for recombinational cloning

657

690

selectable marker

AmpR

ampicillin resistance gene

2622

3281

ssDNA origin

f1 ori

f1 origin for ssDNA production

3928

4368

tag

Flag

3xFlag epitope tag ORF and LoxP region

585

650

trxn termination sequence

T7 term

T7 terminator sequence

1614

1660

Gene

Gene Symbol :

PKP2

Symbol Nomenclature :

PKP2

SYNONYM :

ARVD9

Designation :

plakophilin-2

Full Nomenclature :

plakophilin 2

GENEID :

5318

HGNC :

9024

MIM :

602861

Vega :

OTTHUMG00000169500

Target GenBank:

BC013138

Reference Sequence Alignment

  

HsCD00618128      ---GGGTCCTCGGTCGGTACAGTCTCTGCACCTCGCGCCCCAGCAGGTAAACTAACATTA
NM_004572.3       ATGGCAGCCCCCGGCG-CCCCAGCTGAGTACGGCTACATCCGGACCGTCCTGGGCCAGCA
                     * . ** * * **  .*.. ** :* **  * . . **.*.. **..:  ..**  *

HsCD00618128      TGGATTTTTCCAAGCTACCCAAAATACTCGATGAAGATAAAGAAAGCACATTTGGTTATG
NM_004572.3       GATCCTGGGACAACTGGACAGCTCCAGCCTGGCGCTGCCCTCCGAGGCCAAGCTGAAGCT
                   . . *   .***   ..*...:. *  * .  .. . ..: ..** .**:   *::.  

HsCD00618128      TGCATGGGGTCTCAGGACCTGTGGTTACAGCCTGTGACATGGCGGGTGCAGCCATGTATG
NM_004572.3       GGCGGGGAGCAGCGG--CCGCGGCGGCCAGACAGTCAAGAGCCTGCGGATCCAGG-----
                   **. **.* . *.*  **   *   .***.*:** *..:* * *  *.: *..      

HsCD00618128      AGCTGGTGAGAGTGGGCCACAGCGAATTGG-TTGGAGAGATTATTCGATTGGAGGGTGAC
NM_004572.3       AGCAGGTGCAGCAGACCCTCGCCCGGAAGGGCCGCAGCTCCGTGGGCAACGGAAATCTTC
                  ***:****... :*. **:*. * ..::**   * **. .  :    *: ***..   :*

HsCD00618128      ATGGCTACTATTCAGGTGTATGAAGAAACTTCTGGTGTGTCTGTTGGAGATCCTGTACTT
NM_004572.3       ACCGAACCAGCAGTGTTCCTGAGTATGTCTACAACCTACACTTGGTTGAAAATGATTTTG
                  *  *.:.*:. : :* *  : ..:.:.:**:*:.   : :**     ..*:.  .*: * 

HsCD00618128      CGCACTGGTAAACCCCTCTCTGTAGAGCTTGGTCCTGGCATTATGGGAGCCATTTTTGAT
NM_004572.3       TTGGAGGCCGTTCCCCTGTTCCTAAAACCTATGACATGCTAAAGGCTGGCACAACTG--C
                     .. *  .::***** *   **.*.* *.  .*: **:::* *  .**..:: *    

HsCD00618128      GGTATTCAAAGACCTTTGTCGGATATCAGCAGTCAGACCCAAAGCATCTACATCCCCAGA
NM_004572.3       CACTTATGAAGGTCGCTGGGGAAGAGGAACAGCACAGTACAGCTCCCAGAAGTCCGTGGA
                   . :*: .***. *  **  *.* *  *.*** .... .**.. *. . *..***  .**

HsCD00618128      GGAGTAAACGTGTCTGCTCTTAGCAGAGATATCAAATGGGACTTTACACCTTGCAAAAAC
NM_004572.3       AGAAAGGTCCTTGAGGCATCCT-CTGAGGAGACTGGAG----ATTTCTCCTGACAGCAGC
                  .**.:..:* *  . **:   : *:***.:.:*:..:*    :**:*:*** .**..*.*

HsCD00618128      CTACGGGTTGGTAGTCATATCACTGGCGGAGACATTTATGGAATTGTCAGTGAGAACTCG
NM_004572.3       CCGGAGAGGG-----CTCACTACACGCACAGCGATTACCAGTACAGCCAGAGAAGCCAGG
                  * . .*.  *     *: *  **: **. **. ***:. .*:* :* ***:**...*: *

HsCD00618128      CTTATCAAACACAAAATCATGTTACCCCCACGAAACAGAGGAACTGTAACTTACATTGCT
NM_004572.3       CTGGGCACACCCTG----------CACCACCAAGAAAGCAGGCGGGCCGCCCTCCTAGTG
                  ** . **.**.*:.          *.**..*.*.*.**..*..  * ..*  :*.*:*  

HsCD00618128      CCACCTGGGAATTATGATACCTCTGATGTTGTCTTGGAGCTTGAATTTGAAGGTGTAAAG
NM_004572.3       CCACCGAGATATGCTCGTTCCGAGATCGTGGGGGTCAGCCGTGCTGGCACCACAAGCAGG
                  ***** .*.:** .* .*:** . .: ** *   * .. * **.:   .... :. .*.*

HsCD00618128      GAGAAGTTCACCATGGTGCAAGTATGGCCTGTACGTCAAGTTCGACCTGTCACTGAGAAG
NM_004572.3       -------------CAGCGCCACTTTGACACATACCACAGACAGTACCAGCATGGCTCTGT
                                .* **.* *:**.*. .*** :**.. :  ***:* .:   : :. 

HsCD00618128      CTGCCAGCCAATCATCCTCTGTTGACTGGCCAGAGAGTCCTTGATGCCCTTTTTCCGTGT
NM_004572.3       TAGCGACACCGTTTTTGACAGCATCCCTGCCAACCCGGCCCTGCT-CACGTACCCCAG--
                   :** * .*..* :*  :*:* : .*  ****.. .* ** **.* *.* *:  **.   

HsCD00618128      GTCCAGGGAGGAACTACTGCTATCCCTGGAGCCTTTGGCTGTGGAAAGACAGTGATATCA
NM_004572.3       -GCCAGGGACCAGCCGCAGCATGGGCAACCTCTTGGAGAAGGAGAACTACCTGACGGCAG
                    *******  *.* .*:**::   *:. . * *  .*.:* .***. **.  .. . ..

HsCD00618128      CAGTCTCTATCCAAGTATTCTAACAGTGATGTAATCATCTATGTAGGATGTGGTGAAAGA
NM_004572.3       GGCTCACTGTCGGGCAGGTCAGGCCGCTGGTGCCCCTGCAGCCCGTCACTCAGAACAGGG
                   . **:**.** .. :. **:..*.*  .   .. *: *:.   .  *   .*:..*.*.

HsCD00618128      GGAAATGAGATGTCTGAAGTCCTCCGGGACTTCCCAGAGCTCACAATGGAGGTTGATGGT
NM_004572.3       CTTCCAGGTCCTCCTGGCATCAGAGCTCCTTCCACAGCACCCGCACGCTGAGGGAAGCTG
                    :..:*. .   ***...**. .    . * *.***..* *.**.   ..*  .*    

HsCD00618128      AAGGTAGAGTCAATTATGAAGAGGACAGCTTTGGTAGCCAATACCTCCAATATGCCTGTT
NM_004572.3       GGCCCAGTGTCGCCGTGGATTCCAGCG--GGAGGAGAGCGCACTTGACTGTCGGCCAG--
                  ..   **:***..  : **: . ..*.    :**:.. *..:.   .*:.*. ***:*  

HsCD00618128      GCTGCTAGAGAAGCCTCTATTTATACTGGAATCACACTGTCAGAGTACTTCCGTGACATG
NM_004572.3       ---------GCGGCCGCAGGGGGAAGTGGGAATCTGCTCACTGAGAGAAGCACTTTCACT
                           *..*** *:.   .:* ***.*: . .** :*:***:..: *. * :**  

HsCD00618128      GGCTATCATGTCAGTATGATGGCTGACTCTACCTCTAGATGGGCTGAGGCCCTTAGAGAA
NM_004572.3       GACTCCCAG---CTGGGGAATGCAGACATGGAGATGACTCTG--GAGCGAGCAGTGAGTA
                  *.**. **    .  . **: **:***:  .. :  * :  *   .. *. *: :***:*

HsCD00618128      ATCTCTGGTCGTTTAGCTGAAATGCCTGCAGATAGTGGATATCCAGCCTATCTTGGTGCC
NM_004572.3       TGCTCGAGGCAGACCACATGCTGCCATCCAGGATTTCTGCTGCAG-----------CTAC
                  : *** .* *. : ..*: ..:  *.* ***.:: *  . : *..             .*

HsCD00618128      CGTCTGGCCTCGTTTTATGAACGAGCAGGCAGGGTGAAATGTCTTGGAAATCCTGAAAGA
NM_004572.3       TTTCATACAGCACGAGTGCTTCCAGAAATCTGAAGCTCGGAAGAGGGTTAACCAGCTTCG
                    **: .*. *.  : :  ::* **.*. *:*..  :.. .: : **::*:**:*.:: .

HsCD00618128      GAAGGGAGTGTCAGCATTGTAGGAGCAGTTTCTCCACCTGGTGGTGATTTTTCTGATCCA
NM_004572.3       --------TGGCATCCTCAAG----CTTCTGCAGCTCCTAAAAGTTCAGAATGAAGACGT
                          ** ** *.* .:.    *:  * *: *:***..:.** .: ::* :..:* :

HsCD00618128      GTTACATCTGCCACTCTTGGTATCGTTCAGGTGTTCTGGGGCTTAGATAAGAAACTAGCT
NM_004572.3       ---TCAGCGAGCTGTGTGTGGGGCCTTGAGAAACTTAGTATTTGAAGACAATGACAA-CA
                     :** * . *: * *  * . * ** **.:. * :* .  * *..:.*.:.**:* *:

HsCD00618128      CAACGTAAGCATTTCCCCTCTGTCAATTGGCTCATCAGCTACAGCAAGTATATGCGTGCC
NM_004572.3       AATTGGAGGTGGCTGAACTAAATGGGGTACCTCGGCTGCTCCAGGTG-CTGAAGCAAACC
                  .*: * *.* .  * ..**.:.* .. *. ***. *:***.*** :.  : *:**.:.**

HsCD00618128      TTG--GATGAATACTATGACAAACACTTCACAGAGTTCGTTCCTCTGAGGACGAAAGCTA
NM_004572.3       AGAGACTTGGAGACTAAAAAACAAATAACAGACCATACAGTCAATTTAAGAAGTAGGAAT
                  : .   :**.* ****:.*.*.*.* ::** * ..*:*. **.: * *.**.*:*.*.::

HsCD00618128      AGGAAATTCTGCAGGAAGAAGAAGACCTGGCAGAAATTGTACAGCTTGTGGGAAAGGCTT
NM_004572.3       GGCTGGCCGGGCGCGGTGGCTCACGCCTG------TAATCCCAGCACTTTGGGAGGCCAA
                  .* :..    **. *.:*.. .* .****      :::  .****:  * **.*.* *::

HsCD00618128      CTTTGGCAGAAACAGATAAAATCACTCTGGAGGTAGCAAAACTTATCAAAGATGATTTCC
NM_004572.3       GGCGGGCGGATCACGAGGTCAGGAGTTCGAGACCAGCCTGACCAACATGG----------
                      ***.**:...** .:.*  * *  *...  ***.:.** :* .:..          

HsCD00618128      TACAACAAAATGGATATACTCCTTATGACAGGTTCTGCCCATTCTACAAGACAGTAGGGA
NM_004572.3       -TTTGCTGTGGAATTTGTCATCTAATGACAAACTCAAGAATCTCATGATAACAGAAGCAT
                   : :.*:.:. ..:*: :*: **:******.. **:. ..: **:: *:.****:** .:

HsCD00618128      TGCTGTC-CAACATGATTGCATTTTATGATATGGCTCGTAGAGCTGTTGAAACCACTGCC
NM_004572.3       TGCTTACGCTGACGGAGAATATCATCATCCCCTTTTCTGGGTGGCCTGAAGGAGACTACC
                  **** :* *:... ** :. ** :*.: . .    **  .*:*   * .*... ***.**

HsCD00618128      CAGAGTGACAATAAAATCACATGGTCCATTATTCGTGAGCACATGGGAGACATCCTCTAT
NM_004572.3       CAAAAG--CAAATGGTTTGCTCGATTTTGACATATTCTACAACGTCACTGGATGCCTAAG
                  **.*.   ***::..:* .*: *.*  : :.:*. * :.**..   .. . ** *  :* 

HsCD00618128      AAACTTTCCTCCATGAAATTCAAGGATCCACTGAAAGATGGTGAGGCAAAGATCAAAAGC
NM_004572.3       AAACATGAGTTCTGCTGGCGCTGATGGGAGAAAAGCGATGAGAAG--ATGTGACGGACTC
                  ****:* . * *:  :..  *:.. .  ...:.*..****. .**  *:. .:*..*. *

HsCD00618128      GACTATGCACAACTTCTTGAAGACATGCAGAATGCATTCCGTAGCCTTGAAGATTAGAAG
NM_004572.3       ATTGACTCACTGGTCCATTATGTCAGAGGAACCATTGCAGATTACCAGCCAGATGACAAG
                  .:  *  ***:. * *:* *:*:** . ..*. . :  . .*:.**:  .**** * ***

HsCD00618128      --CCTTGAAGATTACAACTGTGATTTCCTTTTCCTCAGCAAGCTCCTATGTGTATATTTT
NM_004572.3       GCCACGGAGAATTGTGTGTGCATTCTTCATAACCTCTCCTACCAGCTGGAGGCAGAGCTC
                    *.  **..***. .: ** .:* * *:*::****: *:* *: **. . * * *  * 

HsCD00618128      CCTGAATTTCTCATCTCAAACCCTTTGCTTCTTTATTGTGCAGCTTTGAGACTAGTGCCT
NM_004572.3       CCAGAG-------AAATATTCCCAGAATATCTATATTCAAAACCGGAATATCCAG-----
                  **:**.       :.: *::***: :. :***:**** :..* *  :.:.:* **     

HsCD00618128      ATGTGTGTTATTTGTTTCCCTGTTTTTTTGGTAGGTCTTATATAAAACAAACATTCCTTT
NM_004572.3       --------ACTGACAACAACAAAAGTATTGGATG-------TTTTGGCAGTCGAAGCAGG
                          :.* : :: ..*:.:: *:****::*       :*::..**.:*.:: *:  

HsCD00618128      GTTCTAGTGTTGTGAAGGGCCTCCCTCTTCCTTTATCTGAAGTGGTGAATATAGTAAATA
NM_004572.3       --AAAGTAAAAGAGCAATACCAGGACGTGCCGATGCCGGAGGAAAAGAGCAACCCCAAGG
                    :.:. :.::*:*.*. .**:  .  * ** :*. * **.*:..:**. *:.  .** .

HsCD00618128      TACATTCTGGTTACACTACTGTAAACTTGTATGTAGGGTGATGACCCTCTTTGTCCTAGG
NM_004572.3       GCGTGGAGTGGCTGTGGCATTCCATTGTTATAAGGATGTATCTGTCCTTGATCGCCAAAA
                   . :  .  *  : :  ..*  .*:  * :::. .. **.:  . ***  :*  **:*..

HsCD00618128      TGTACCCTTTCCTCATCTCTATTAAATTGTAAACAGGACTACTGCATGTACTCTCTTTGC
NM_004572.3       GTGTCCG---CAACTACACACAAGAAGCATCCTTAGGAGCTCTGCAGAACCTCACG----
                     :**    *.:*::*:*:.::.**  .*..: ****  :***** .:.***:*     

HsCD00618128      AGTGAATTTGGAATGGAAGGCCAGGTTTCTATAACTTTTGAACAGGTACTTTGTGCAATG
NM_004572.3       -------------------GCCGG-----------AAGTGGACCAATGCCGACATCAGTG
                                     ***.*           :: **.**...*.*  : : **.**

HsCD00618128      ACTCAATTTCTATTGTGGTAAGCTCATTGGCAGCTTAGCATTTTGCAAAGGAATTGCTTT
NM_004572.3       GCTCAGACAGTTGTCCAGAAGGAAAGTGGCCTGCAGCACACCCGAAAGATGCTGCATGTT
                  .****.: : *: *  .*:*.*.:..* * *:**: ..**    ..*.* *.:  .  **

HsCD00618128      GCAGGAAATATTTAATTTTCAAAAACATAATGATTAATGTTCCAATTATGCAACACTTCC
NM_004572.3       GGTG------------------------------------ACCCAAGTGTGAAAAAGACA
                  * :*                                    :**.*: :   **.*. :*.

HsCD00618128      CCCAGTATAAATCAGGAATGTTTGTGAGAAACCATTGGGAACTATACTCTTTTTATTTTT
NM_004572.3       GCCATCTCGCTGCTG---------------------AGGAATCTGTCCCGGAATCTTTCT
                   ***  : ..: *:*                     .****  : :* *  ::*.*** *

HsCD00618128      ATTTTTTATTTTTTTTATTATTTTTTTTTTGGGGACGGAGTGTCCCTCTTGTTGCCCAGG
NM_004572.3       CTGCAGAATGAAATTGCCAAAGAAACTCTCCCTGATTTGGTTTCCATCAT----TCCTGA
                  .*  : :** :::** . :*: ::: * *    **   .** ***.**:*     **:*.

HsCD00618128      CTGGAGTGCAATGGCGTGATCTTGGCTCACTGCAGCCTTCGCCTCCCGGGTTCAAGTGAT
NM_004572.3       CACAGTCCCGAGTACTG-ACCTTCTCATTGAAACTACAGCCTCTGCCTGTTACACATTGA
                  *: ..   *.*  .*   * ***  *: : :... .*: *  ** ** * *:**..* .:

HsCD00618128      TCTCCTGCCTCAGCCTCCCGAGTAGCTGGGATTACAGGCATGCTCCACCATGCCCAGCTA
NM_004572.3       ACAACATAATCCAAAACAG----TTACCAGAATGCACGCGACCTTCTAAACACCGG----
                  :*:.*: ..**....:*.     : .  .**:*.** **.: ** *:..* .** .    

HsCD00618128      ATTTTGTATTTTTAGTAGAAACGGGGTTTCACCATATTGGTCAGGCTGGTCTCGAACTCC
NM_004572.3       ------------GGGCATCCAGAAAATTATGGCCATTAGTGCAGGCGATGCCTATGCCTC
                               .* * ..* ....**: . *.::*:*  ***** .  *  .:.*  *

HsCD00618128      AGACCTCAGGTGATCCGCCCACCTCGGCCTCCCAAACTGCTGGGATTACAGGCGTGAGCC
NM_004572.3       CAACAAAG-CAAGTAAAGCTGCTTCCGTCCTTCTGTATTCTCTGTGGGCACACACGG--A
                  ..**.:..  :..*... * .* ** * *   *:.:.* **  *:  .** .*. *.  .

HsCD00618128      ACCGCGCCTGGCCAGGGACTATACTCTTTTTAAAATAGACATTTGTGGGGCTCACACAAT
NM_004572.3       ACTGCATCATGCCTACAAGAAGGCTCAGTTTAAGAAGACAGATTTTGTCAACAGCCGGAC
                  ** **. *: ***:. .* :* .***: *****.*:.....:** **  .. ..*. .* 

HsCD00618128      ATATGAAATAGTACCCTCTAAAAAAAAAAAAAAAAA
NM_004572.3       TGCCAAAGCCTACCACTCCCTTAAAGACTGA-----
                  : . .**. . :.*.*** .::***.*.:.*

Pathways

Reactome :

Disease

Reactome :

Insulin receptor recycling

Reactome :

Iron uptake and transport

Reactome :

Latent infection of Homo sapiens with Mycobacterium tuberculosis

Reactome :

Phagosomal maturation (early endosomal stage)

Reactome :

Signal Transduction

Reactome :

Signaling by Insulin receptor

Reactome :

Transferrin endocytosis and recycling

Reactome :

Transmembrane transport of small molecules

Protein

HPRD :

04177

Clone

Cloning Information :

654389

HIP Master Clone ID :

28177

IMAGE ID :

4454722

Original Clone ID :

HsCD00299505

Original Clone ID :

FLH232116.01L

Organism

Species Specific ID:

523

Genome

Chromosome :

Map Location :

12p11

Ensembl :

ENSG00000057294

ATP6V1A (H. sapiens )In pLDNT7_nFLAG (Flag-taggedIn vitro expression vector)

Coding Sequence Details

Recommended Growth Condition:

Vector Features:

Gene

Reference Sequence Alignment

Pathways

Protein

Clone

Organism

Genome

ATP6V1A (H. sapiens )
In pLDNT7_nFLAG (Flag-tagged
In vitro expression vector)