Gene:
Gene Symbol:
MTM1
Gene Name:
myotubularin 1
Original Clone ID:
HsCD00298343
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pLDNT7_nFLAG
Format:
CLOSED
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00618152
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 1812nts
Open reading frame: 36 to 1847
AGCGGGGTGGCGCCCGGAGCCCGAGAGTTTCCAGGATGGCTTCTGCATCAACTTCTAAAT
ATAATTCACACTCCTTGGAGAATGAGTCTATTAAGAGGACGTCTCGAGATGGAGTCAATC
GAGATCTCACTGAGGCTGTTCCTCGACTTCCAGGAGAAACACTAATCACTGACAAAGAAG
TTATTTACATATGTCCTTTCAATGGCCCCATTAAGGGAAGAGTTTACATCACAAATTATC
GTCTTTATTTAAGAAGTTTGGAAACGGATTCTTCTCTAATACTTGATGTTCCTCTGGGTG
TGATCTCGAGAATTGAAAAAATGGGAGGCGCGACAAGTAGAGGAGAAAATTCCTATGGTC
TAGATATTACTTGTAAAGACATGAGAAACCTGAGGTTCGCTTTGAAACAGGAAGGCCACA
GCAGAAGAGATATGTTTGAGATCCTCACGAGATACGCGTTTCCCCTGGCTCACAGTCTGC
CATTATTTGCATTTTTAAATGAAGAAAAGTTTAACGTGGATGGATGGACAGTTTACAATC
CAGTGGAAGAATACAGGAGGCAGGGCTTGCCCAATCACCATTGGAGAATAACTTTTATTA
ATAAGTGCTATGAGCTCTGTGACACTTACCCTGCTCTTTTGGTGGTTCCGTATCGTGCCT
CAGATGATGACCTCCGGAGAGTTGCAACTTTTAGGTCCCGAAATCGAATTCCAGTGCTGT
CATGGATTCATCCAGAAAATAAGACGGTCATTGTGCGTTGCAGTCAGCCTCTTGTCGGTA
TGAGTGGGAAACGAAATAAAGATGATGAGAAATATCTCGATGTTATCAGGGAGACTAATA
AACAAATTTCTAAACTCACCATTTATGATGCAAGACCCAGCGTAAATGCAGTGGCCAACA
AGGCAACAGGAGGAGGATATGAAAGTGATGATGCATATCATAACGCCGAACTTTTCTTCT
TAGACATTCATAATATTCATGTTATGCGGGAATCTTTAAAAAAAGTGAAGGACATTGTTT
ATCCTAATGTAGAAGAATCTCATTGGTTGTCCAGTTTGGAGTCTACTCATTGGTTAGAAC
ATATCAAGCTCGTTTTGACAGGAGCCATTCAAGTAGCAGACAAAGTTTCTTCAGGGAAGA
GTTCAGTGCTTGTGCATTGCAGTGACGGATGGGACAGGACTGCTCAGCTGACATCCTTGG
CCATGCTGATGTTGGATAGCTTCTATAGGAGCATTGAAGGGTTCGAAATACTGGTACAAA
AAAAATGGATAAGTTTTGGACATAAATTTGCATCTCGAATAGGTCATGGTGATAAAAACC
ACACCGATGCTGACCGTTCTCCTATTTTTCTCCAGTTTATTGATTGTGTGTGGCAAATGT
CAAAACAGTTCCCTACAGCTTTTGAATTCAATGAACAATTTTTGATTATAATTTTGGATC
ATCTGTATAGTTGCCGATTTGGTACTTTCTTATTCAACTGTGAATCTGCTCGAGAAAGAC
AGAAGGTTACAGAAAGGACTGTTTCTTTATGGTCACTGATAAACAGTAATAAAGAAAAAT
TCAAAAACCCCTTCTATACTAAAGAAATCAATCGAGTTTTATATCCAGTTGCCAGTATGC
GTCACTTGGAACTCTGGGTGAATTACTACATTAGATGGAACCCCAGGATCAAGCAACAAC
AGCCGAATCCAGTGGAGCAGCGTTACATGGAGCTCTTAGCCTTACGCGACGAATACATAA
AGCGGCTTGAGGAACTGCAGCTCGCCAACTCTGCCAAGCTTTCTGATCCCCCAACTTCAC
CTTCCAGTCCTTCGCAAATGATGCCCCATGTGCAAACTCACTTCTGAGGGGGGACCCTGG
CACCGCATTAGAGCTCGAAATAAAGGCGATAGCTGACTTTCATTTGGGGCATTTGTAAAA
AGTAGATTAAAATATTTGCCTCCATGTAGAACTTGAACTAACATAATCTTAAACTCTTGA
ATATGTGCCTTCTAGAATACATATTACAAGAAAACTACAGGGTCCACACGGCAATCAGAA
GAAAGGAGCTGAGATGAGGTTTTGGAAAACCCTGACACCTTTAAAAAGCAGTTTTTGAAA
GACAAAATTTAGATTTAATTTACGTCTTGAGAAATACTATATATACAATATATATTTTGT
GGGCTTAATTGAAACAACATTATTTTAAAATCAAAGGGGATATATGTTTGTGGAATGGAT
TTTCCTGAAGCTGCTTAACAGTTGCTTTGGATTCTCTAAGATGAATCCAAATGTGAAAGA
TGCATGTTACTGCCAAAACCAAATTGAGCTCAGCTTCCTAGGCATTACCCAAAAGCAAGG
TGTTTAAGTAATTGCCAGCTTTTATACCATCATGAGTGGTGACTTAAGGAGAAATAGCTG
TATAGATGAGTTTTTCATTATTTGGAAATTTAGGGGTAGAAAATGTTTTCCCCTAATTTT
CCAGAGAAGCCTATTTTTATATTTTTAAAAAACTGACAGGGCCCAGTTAAATATGATTTG
CATTTTTTAAATTTGCCAGTTTTATTTTCTAAATTCTTTCATGAGCTTGCCTAAAATTCG
GAATGGTTTTCGGGTTGTGGCAAACCCCAAAGAGAGCACTGTCCAAGGATGTCGGGAGCA
TCCTGCTGCTTAGGGGAATGTTTTCGCAAATGTTGCTCTAGTCAGTCCAGCTCATCTGCC
AAAATGTAGGGCTACCGTCTTGGATGCATGAGCTATTGCTAGAGCATCATCCTTAGAAAT
CAGTGCCCCAGATGTACATGTGTTGAGCGTATTCTTGAAAGTATTGTGTTTATGCATTTC
AATTTCAATGGTGTTGGCTTCCCCTCCCCACCCCACGCGTGCATAAAAACTGGTTCTACA
AATTTTTTACTTGAAGTACCAGGCCGTTTGCTTTTTCAGGTTGTTTTGTTTTATAGTATT
AAGTGAAATTTTAAATGCACAGTTCTATTTGCTATCTGAACTAATTCATTTATTAAGTAT
ATTTGTAAAAGCTAAGGCTCGAGTTAAAACAATGAAGTGTTTTACAATGATTTGTAAAGG
ACTATTTATAACTAATATGGTTTTGTTTTCAATGAATTAAGAAAGATTAAATATATCTTT
GTAAATTATTTTATGTCATAGTTTAATTGGTCTACCAAGTAAGACATCTCAAATACAGTA
GTATAATGTATGAATTTTGTAAGTATAAGAAATTTTATTAGACATTCTCTTACTTTTTGT
AAATGCTGTAAATATTTCATAAATTAACAAAGTGTCACTCCATAAAAAGAAAGCTAATAC
TAATAGCCTAAAAGATTTTGTGAAATTTCATGAAAACTTTTTAATGGCAATAATGACTAA
AGACCTGCTGTAATAAATGTATTAACTGAAAAAAAAAAAAAAAA
Protein sequence predicted based on nucleotide sequence
MASASTSKYNSHSLENESIKRTSRDGVNRDLTEAVPRLPGETLITDKEVIYICPFNGPIK GRVYITNYRLYLRSLETDSSLILDVPLGVISRIEKMGGATSRGENSYGLDITCKDMRNLR FALKQEGHSRRDMFEILTRYAFPLAHSLPLFAFLNEEKFNVDGWTVYNPVEEYRRQGLPN HHWRITFINKCYELCDTYPALLVVPYRASDDDLRRVATFRSRNRIPVLSWIHPENKTVIV RCSQPLVGMSGKRNKDDEKYLDVIRETNKQISKLTIYDARPSVNAVANKATGGGYESDDA YHNAELFFLDIHNIHVMRESLKKVKDIVYPNVEESHWLSSLESTHWLEHIKLVLTGAIQV ADKVSSGKSSVLVHCSDGWDRTAQLTSLAMLMLDSFYRSIEGFEILVQKKWISFGHKFAS RIGHGDKNHTDADRSPIFLQFIDCVWQMSKQFPTAFEFNEQFLIIILDHLYSCRFGTFLF NCESARERQKVTERTVSLWSLINSNKEKFKNPFYTKEINRVLYPVASMRHLELWVNYYIR WNPRIKQQQPNPVEQRYMELLALRDEYIKRLEELQLANSAKLSDPPTSPSSPSQMMPHVQ THF*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Host Type: bacterial Marker: chloramphenicol
Host Type: bacterial Marker: chloramphenicol
Bacterial Selection Condition:
100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition:
Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments:
Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pLDNT7_nFLAG
Synonyms:
pLDNT7-Flag
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Vector for in vitro translation with mammalian extracts, adds N-terminal Flag epitope tag; amp resistance; recombinational cloning
Comments:
None
Size (bp):
4557
Parent Vector:
None
Properties:
Creator, acceptor (destination), in vitro transcription, loxP, ssDNA production, with tag/fusion/marker
Author Name:
Daniel Schiwek
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE1 ori
ColE1 origin of replication
1842
2524
promoter
T7
T7 promoter
4462
4479
recombination site
LoxP
LoxP site for recombinational cloning
657
690
selectable marker
AmpR
ampicillin resistance gene
2622
3281
ssDNA origin
f1 ori
f1 origin for ssDNA production
3928
4368
tag
Flag
3xFlag epitope tag ORF and LoxP region
585
650
trxn termination sequence
T7 term
T7 terminator sequence
1614
1660
