Gene:
Gene Symbol:
EPHA1
Gene Name:
EPH receptor A1
Original Clone ID:
FLH231607.01L
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pLDNT7_nFLAG
Format:
CLOSED
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00618242
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 2931nts
Open reading frame: 88 to 3018
GCCCCCGCCCGGCCCGCCCCGCTCTCCTAGTCCCTTGCAACCTGGCGCTGCCATCCGGGC
CACTGTCCCAGGTCCCGGCCCGGAGCTATGGAGCGGCGCTGGCCCCTGGGGCTAGGGCTG
GTGCTGCTGCTCTGCGCCCCGCTGCCCCCGGGGGCGCGCGCCAAGGAAGTTACTCTGATG
GACACAAGCAAGGCACAGGGAGAGCTGGGCTGGCTGCTGGATCCCCCAAAAGATGGGTGG
AGTGAACAGCAACAGATACTGAATGGGACACCCCTGTACATGTACCAGGACTGCCCAATG
CAAGGACGCAGAGACACTGACCACTGGCTTCGCTCCAATTGGATCTACCGCGGGGAGGAG
GCTTCCCGCGTCCACGTGGAGCTGCAGTTCACCGTGCGGGACTGCAAGAGTTTCCCTGGG
GGAGCCGGGCCTCTGGGCTGCAAGGAGACCTTCAACCTTCTGTACATGGAGAGTGACCAG
GATGTGGGCATTCAGCTCCGACGGCCCTTGTTCCAGAAGGTAACCACGGTGGCTGCAGAC
CAGAGCTTCACCATTCGAGACCTTGCGTCTGGCTCCGTGAAGCTGAATGTGGAGCGCTGC
TCTCTGGGCCGCCTGACCCGCCGTGGCCTCTACCTCGCTTTCCACAACCCGGGTGCCTGT
GTGGCCCTGGTGTCTGTCCGGGTCTTCTACCAGCGCTGTCCTGAGACCCTGAATGGCTTG
GCCCAATTCCCAGACACTCTGCCTGGCCCCGCTGGGTTGGTGGAAGTGGCGGGGACCTGC
TTGCCCCACGCGCGGGCCAGCCCCAGGCCCTCAGGTGCACCCCGCATGCACTGCAGCCCT
GATGGCGAGTGGCTGGTGCCTGTAGGACGGTGCCACTGTGAGCCTGGCTATGAGGAAGGT
GGCAGTGGCGAAGCATGTGTTGCCTGCCCTAGCGGCTCCTACCGGATGGACATGGACACA
CCCCATTGTCTCACGTGCCCCCAGCAGAGCACTGCTGAGTCTGAGGGGGCCACCATCTGT
ACCTGTGAGAGCGGCCATTACAGAGCTCCCGGGGAGGGCCCCCAGGTGGCATGCACAGGT
CCCCCCTCGGCCCCCCGAAACCTGAGCTTCTCTGCCTCAGGGACTCAGCTCTCCCTGCGT
TGGGAACCCCCAGCAGATACGGGGGGACGCCAGGATGTCAGATACAGTGTGAGGTGTTCC
CAGTGTCAGGGCACAGCACAGGACGGGGGGCCCTGCCAGCCCTGTGGGGTGGGCGTGCAC
TTCTCGCCGGGGGCCCGGGCGCTCACCACACCTGCAGTGCATGTCAATGGCCTTGAACCT
TATGCCAACTACACCTTTAATGTGGAAGCCCAAAATGGAGTGTCAGGGCTGGGCAGCTCT
GGCCATGCCAGCACCTCAGTCAGCATCAGCATGGGGCATGCAGAGTCACTGTCAGGCCTG
TCTCTGAGACTGGTGAAGAAAGAACCGAGGCAACTAGAGCTGACCTGGGCGGGGTCCCGG
CCCCGAAGCCCTGGGGCGAACCTGACCTATGAGCTGCACGTGCTGAACCAGGATGAAGAA
CGGTACCAGATGGTTCTAGAACCCAGGGTCTTGCTGACAGAGCTGCAGCCTGACACCACA
TACATCGTCAGAGTCCGAATGCTGACCCCACTGGGTCCTGGCCCTTTCTCCCCTGATCAT
GAGTTTCGGACCAGCCCACCAGTGTCCAGGGGCCTGACTGGAGGAGAGATTGTAGCCGTC
ATCTTTGGGCTGCTGCTTGGTGCAGCCTTGCTGCTTGGGATTCTCGTTTTCCGGTCCAGG
AGAGCCCAGCGGCAGAGGCAGCAGAGGCAGCGTGACCGCGCCACCGATGTGGATCGAGAG
GACAAGCTGTGGCTGAAGCCTTATGTGGACCTCCAGGCATACGAGGACCCTGCACAGGGA
GCCTTGGACTTTACCCGGGAGCTTGATCCAGCGTGGCTGATGGTGGACACTGTCATAGGA
GAAGGAGAGTTTGGGGAAGTGTATCGAGGGACCCTGAGGCTCCCCAGCCAGGACTGCAAG
ACTGTGGCCATTAAGACCTTAAAAGACACATCCCCAGGTGGCCAGTGGTGGAACTTCCTT
CGAGAGGCAACTATCATGGGCCAGTTTAGCCACCCGCATATTCTGCATCTGGAAGGCGTC
GTCACAAAGCGAAAGCCGATCATGATCATCACAGAATTTATGGAGAATGGAGCCCTGGAT
GCCTTCCTGAGGGAGCGGGAGGACCAGCTGGTCCCTGGGCAGCTAGTGGCCATGCTGCAG
GGCATAGCATCTGGCATGAACTACCTCAGTAATCACAATTATGTCCACCGGGACCTGGCT
GCCAGAAACATCTTGGTGAATCAAAACCTGTGCTGCAAGGTGTCTGACTTTGGCCTGACT
CGCCTCCTGGATGACTTTGATGGCACATACGAAACCCAGGGAGGAAAGATCCCTATCCGT
TGGACAGCCCCTGAAGCCATTGCCCATCGGATCTTCACCACAGCCAGCGATGTGTGGAGC
TTTGGGATTGTGATGTGGGAGGTGCTGAGCTTTGGGGACAAGCCTTATGGGGAGATGAGC
AATCAGGAGGTTATGAAGAGCATTGAGGATGGGTACCGGTTGCCCCCTCCTGTGGACTGC
CCTGCCCCTCTGTATGAGCTCATGAAGAACTGCTGGGCATATGACCGTGCCCGCCGGCCA
CACTTCCAGAAGCTTCAGGCACATCTGGAGCAACTGCTTGCCAACCCCCACTCCCTGCGG
ACCATTGCCAACTTTGACCCCAGGGTGACTCTTCGCCTGCCCAGCCTGAGTGGCTCAGAT
GGGATCCCGTATCGAACCGTCTCTGAGTGGCTCGAGTCCATACGCATGAAACGCTACATC
CTGCACTTCCACTCGGCTGGGCTGGACACCATGGAGTGTGTGCTGGAGCTGACCGCTGAG
GACCTGACGCAGATGGGAATCACACTGCCCGGGCACCAGAAGCGCATTCTTTGCAGTATT
CAGGGATTCAAGGACTGATCCCTCCTCTCACCCCATGCCCAATCAGGGTGCAAGGAGCAA
GGACGGGGCCAAGGTCGCTCATGGTCACTCCCTGCGCCCCTTCCCACAACCTGCCAGACT
AGGCTATCGGTGCTGCTTCTGCCCACTTTCAGGAGAACCCTGCTCTGCACCCCAGAAAAC
CTCTTTGTTTTAAAAGGGAGGTGGGGGTAGAAGTAAAAGGATGATCATGGGAGGGAGCTG
AGGGGTTAATATATATACATACATACACATATATATATTTTTGTAAATAAACAGGAACTG
ATTTTCTGCCTCCATCCCACCCATGAGGGCTGCAGGCACTACAAAAGAGCTGACTACTGA
GAA
Protein sequence predicted based on nucleotide sequence
MERRWPLGLGLVLLLCAPLPPGARAKEVTLMDTSKAQGELGWLLDPPKDGWSEQQQILNG TPLYMYQDCPMQGRRDTDHWLRSNWIYRGEEASRVHVELQFTVRDCKSFPGGAGPLGCKE TFNLLYMESDQDVGIQLRRPLFQKVTTVAADQSFTIRDLASGSVKLNVERCSLGRLTRRG LYLAFHNPGACVALVSVRVFYQRCPETLNGLAQFPDTLPGPAGLVEVAGTCLPHARASPR PSGAPRMHCSPDGEWLVPVGRCHCEPGYEEGGSGEACVACPSGSYRMDMDTPHCLTCPQQ STAESEGATICTCESGHYRAPGEGPQVACTGPPSAPRNLSFSASGTQLSLRWEPPADTGG RQDVRYSVRCSQCQGTAQDGGPCQPCGVGVHFSPGARALTTPAVHVNGLEPYANYTFNVE AQNGVSGLGSSGHASTSVSISMGHAESLSGLSLRLVKKEPRQLELTWAGSRPRSPGANLT YELHVLNQDEERYQMVLEPRVLLTELQPDTTYIVRVRMLTPLGPGPFSPDHEFRTSPPVS RGLTGGEIVAVIFGLLLGAALLLGILVFRSRRAQRQRQQRQRDRATDVDREDKLWLKPYV DLQAYEDPAQGALDFTRELDPAWLMVDTVIGEGEFGEVYRGTLRLPSQDCKTVAIKTLKD TSPGGQWWNFLREATIMGQFSHPHILHLEGVVTKRKPIMIITEFMENGALDAFLREREDQ LVPGQLVAMLQGIASGMNYLSNHNYVHRDLAARNILVNQNLCCKVSDFGLTRLLDDFDGT YETQGGKIPIRWTAPEAIAHRIFTTASDVWSFGIVMWEVLSFGDKPYGEMSNQEVMKSIE DGYRLPPPVDCPAPLYELMKNCWAYDRARRPHFQKLQAHLEQLLANPHSLRTIANFDPRV TLRLPSLSGSDGIPYRTVSEWLESIRMKRYILHFHSAGLDTMECVLELTAEDLTQMGITL PGHQKRILCSIQGFKD*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Host Type: bacterial Marker: chloramphenicol
Host Type: bacterial Marker: chloramphenicol
Bacterial Selection Condition:
100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition:
Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments:
Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pLDNT7_nFLAG
Synonyms:
pLDNT7-Flag
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Vector for in vitro translation with mammalian extracts, adds N-terminal Flag epitope tag; amp resistance; recombinational cloning
Comments:
None
Size (bp):
4557
Parent Vector:
None
Properties:
Creator, acceptor (destination), in vitro transcription, loxP, ssDNA production, with tag/fusion/marker
Author Name:
Daniel Schiwek
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE1 ori
ColE1 origin of replication
1842
2524
promoter
T7
T7 promoter
4462
4479
recombination site
LoxP
LoxP site for recombinational cloning
657
690
selectable marker
AmpR
ampicillin resistance gene
2622
3281
ssDNA origin
f1 ori
f1 origin for ssDNA production
3928
4368
tag
Flag
3xFlag epitope tag ORF and LoxP region
585
650
trxn termination sequence
T7 term
T7 terminator sequence
1614
1660
