DNASU Plasmid | RRP8 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: RRP8

Gene Name: ribosomal RNA processing 8, methyltransferase, homolog (yeast)

Original Clone ID:

ccsbBroadEn_02756

Keyword:

PERFECT_MATCH

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00622824

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1371nts

Open reading frame: 1 to 1371

Reference Sequence Alignment

ATGTTCGAAGAGCCTGAGTGGGCCGAGGCGGCCCCAGTAGCCGCGGGCCTTGGGCCCGTA
ATCTCACGACCTCCGCCTGCGGCCTCCTCGCAAAACAAGGGCTCCAAGCGCCGCCAGCTC
TTGGCCACATTACGGGCCCTAGAGGCAGCATCTCTTTCCCAGCATCCCCCCAGCCTATGT
ATAAGTGACTCTGAGGAGGAGGAGGAGGAAAGGAAGAAGAAATGCCCCAAAAAGGCATCA
TTTGCCAGTGCCTCTGCTGAAGTAGGGAAGAAAGGGAAGAAGAAATGTCAAAAACAGGGC
CCACCTTGCAGTGACTCTGAGGAAGAAGTAGAAAGGAAGAAGAAATGCCACAAACAGGCT
CTTGTTGGCAGTGACTCTGCTGAAGATGAGAAAAGAAAGAGGAAATGCCAGAAACATGCC
CCTATAAATTCAGCCCAGCACCTGGACAATGTTGACCAAACAGGTCCCAAAGCCTGGAAG
GGTAGTACTACAAATGATCCACCAAAGCAAAGCCCTGGGTCCACTTCCCCTAAACCCCCT
CATACATTAAGCCGCAAGCAGTGGCGGAACCGGCAAAAGAATAAGAGAAGATGTAAGAAC
AAGTTTCAGCCACCTCAGGTGCCAGACCAGGCCCCAGCTGAGGCCCCCACAGAGAAGACA
GAGGTGTCTCCTGTTCCCAGGACAGACAGCCATGAGGCTCGGGCAGGGGCTTTGCGAGCC
CGCATGGCACAGCGGCTGGATGGGGCCCGATTTCGCTACCTCAATGAACAGTTGTACTCA
GGGCCCAGCAGTGCTGCACAGCGTCTCTTCCAGGAAGACCCTGAGGCTTTTCTTCTCTAC
CACCGCGGCTTCCAGAGCCAAGTGAAGAAGTGGCCACTGCAGCCAGTGGACCGCATCGCC
AGGGATCTTCGCCAGCGGCCTGCATCCCTAGTGGTGGCTGACTTCGGCTGTGGGGATTGC
CGCTTGGCTTCAAGTATCCGGAACCCTGTGCATTGCTTTGACTTGGCTTCTCTGGACCCT
AGGGTCACTGTGTGTGACATGGCCCAGGTTCCTCTGGAGGATGAGTCTGTGGATGTGGCT
GTGTTTTGCCTTTCACTGATGGGAACCAACATCAGGGACTTCCTAGAGGAGGCAAATAGA
GTACTGAAGCCAGGGGGTCTCCTGAAAGTGGCTGAGGTCAGCAGCCGCTTTGAGGATGTT
CGAACCTTTCTGCGGGCTGTGACCAAGCTAGGCTTCAAGATTGTCTCCAAGGACCTGACC
AACAGCCATTTCTTCTTGTTTGATTTCCAAAAGACTGGGCCCCCTCTGGTAGGGCCCAAG
GCTCAGCTTTCAGGCCTGCAGCTTCAGCCATGTCTCTACAAGCGCAGGTGA

Protein sequence predicted based on nucleotide sequence

MFEEPEWAEAAPVAAGLGPVISRPPPAASSQNKGSKRRQLLATLRALEAASLSQHPPSLC
ISDSEEEEEERKKKCPKKASFASASAEVGKKGKKKCQKQGPPCSDSEEEVERKKKCHKQA
LVGSDSAEDEKRKRKCQKHAPINSAQHLDNVDQTGPKAWKGSTTNDPPKQSPGSTSPKPP
HTLSRKQWRNRQKNKRRCKNKFQPPQVPDQAPAEAPTEKTEVSPVPRTDSHEARAGALRA
RMAQRLDGARFRYLNEQLYSGPSSAAQRLFQEDPEAFLLYHRGFQSQVKKWPLQPVDRIA
RDLRQRPASLVVADFGCGDCRLASSIRNPVHCFDLASLDPRVTVCDMAQVPLEDESVDVA
VFCLSLMGTNIRDFLEEANRVLKPGGLLKVAEVSSRFEDVRTFLRAVTKLGFKIVSKDLT
NSHFFLFDFQKTGPPLVGPKAQLSGLQLQPCLYKRR*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

RRP8

Symbol Nomenclature :

RRP8

SYNONYM :

KIAA0409

Designation :

RRP8 methyltransferase homolog|cerebral protein 1|nucleomethylin|ribosomal RNA-processing protein 8

Full Nomenclature :

ribosomal RNA processing 8, methyltransferase, homolog (yeast)

GENEID :

23378

Locus Tag :

hucep-1

HGNC :

29030

Target GenBank:

BC001071.2

Reference Sequence Alignment

  

HsCD00622824      ATGTTCGAAGAGCCTGAGTGGGCCGAGGCGGCCCCAGTAGCCGCGGGCCTTGGGCCCGTA
NM_015324.3       ATGTTCGAAGAGCCTGAGTGGGCCGAGGCGGCCCCAGTAGCCGCGGGCCTTGGGCCCGTA
                  ************************************************************

HsCD00622824      ATCTCACGACCTCCGCCTGCGGCCTCCTCGCAAAACAAGGGCTCCAAGCGCCGCCAGCTC
NM_015324.3       ATCTCACGACCTCCGCCTGCGGCCTCCTCGCAAAACAAGGGCTCCAAGCGCCGCCAGCTC
                  ************************************************************

HsCD00622824      TTGGCCACATTACGGGCCCTAGAGGCAGCATCTCTTTCCCAGCATCCCCCCAGCCTATGT
NM_015324.3       TTGGCCACATTACGGGCCCTAGAGGCAGCATCTCTTTCCCAGCATCCCCCCAGCCTATGT
                  ************************************************************

HsCD00622824      ATAAGTGACTCTGAGGAGGAGGAGGAGGAAAGGAAGAAGAAATGCCCCAAAAAGGCATCA
NM_015324.3       ATAAGTGACTCTGAGGAGGAGGAGGAGGAAAGGAAGAAGAAATGCCCCAAAAAGGCATCA
                  ************************************************************

HsCD00622824      TTTGCCAGTGCCTCTGCTGAAGTAGGGAAGAAAGGGAAGAAGAAATGTCAAAAACAGGGC
NM_015324.3       TTTGCCAGTGCCTCTGCTGAAGTAGGGAAGAAAGGGAAGAAGAAATGTCAAAAACAGGGC
                  ************************************************************

HsCD00622824      CCACCTTGCAGTGACTCTGAGGAAGAAGTAGAAAGGAAGAAGAAATGCCACAAACAGGCT
NM_015324.3       CCACCTTGCAGTGACTCTGAGGAAGAAGTAGAAAGGAAGAAGAAATGCCACAAACAGGCT
                  ************************************************************

HsCD00622824      CTTGTTGGCAGTGACTCTGCTGAAGATGAGAAAAGAAAGAGGAAATGCCAGAAACATGCC
NM_015324.3       CTTGTTGGCAGTGACTCTGCTGAAGATGAGAAAAGAAAGAGGAAATGCCAGAAACATGCC
                  ************************************************************

HsCD00622824      CCTATAAATTCAGCCCAGCACCTGGACAATGTTGACCAAACAGGTCCCAAAGCCTGGAAG
NM_015324.3       CCTATAAATTCAGCCCAGCACCTGGACAATGTTGACCAAACAGGTCCCAAAGCCTGGAAG
                  ************************************************************

HsCD00622824      GGTAGTACTACAAATGATCCACCAAAGCAAAGCCCTGGGTCCACTTCCCCTAAACCCCCT
NM_015324.3       GGTAGTACTACAAATGATCCACCAAAGCAAAGCCCTGGGTCCACTTCCCCTAAACCCCCT
                  ************************************************************

HsCD00622824      CATACATTAAGCCGCAAGCAGTGGCGGAACCGGCAAAAGAATAAGAGAAGATGTAAGAAC
NM_015324.3       CATACATTAAGCCGCAAGCAGTGGCGGAACCGGCAAAAGAACAAGAGAAGATGTAAGAAC
                  ***************************************** ******************

HsCD00622824      AAGTTTCAGCCACCTCAGGTGCCAGACCAGGCCCCAGCTGAGGCCCCCACAGAGAAGACA
NM_015324.3       AAGTTTCAGCCACCTCAGGTGCCAGACCAGGCCCCAGCTGAGGCCCCCACAGAGAAGACA
                  ************************************************************

HsCD00622824      GAGGTGTCTCCTGTTCCCAGGACAGACAGCCATGAGGCTCGGGCAGGGGCTTTGCGAGCC
NM_015324.3       GAGGTGTCTCCTGTTCCCAGGACAGACAGCCATGAGGCTCGGGCAGGGGCTTTGCGAGCC
                  ************************************************************

HsCD00622824      CGCATGGCACAGCGGCTGGATGGGGCCCGATTTCGCTACCTCAATGAACAGTTGTACTCA
NM_015324.3       CGCATGGCACAGCGGCTGGATGGGGCCCGATTTCGCTACCTCAATGAACAGTTGTACTCA
                  ************************************************************

HsCD00622824      GGGCCCAGCAGTGCTGCACAGCGTCTCTTCCAGGAAGACCCTGAGGCTTTTCTTCTCTAC
NM_015324.3       GGGCCCAGCAGTGCTGCACAGCGTCTCTTCCAGGAAGACCCTGAGGCTTTTCTTCTCTAC
                  ************************************************************

HsCD00622824      CACCGCGGCTTCCAGAGCCAAGTGAAGAAGTGGCCACTGCAGCCAGTGGACCGCATCGCC
NM_015324.3       CACCGCGGCTTCCAGAGCCAAGTGAAGAAGTGGCCACTGCAGCCAGTGGACCGCATCGCC
                  ************************************************************

HsCD00622824      AGGGATCTTCGCCAGCGGCCTGCATCCCTAGTGGTGGCTGACTTCGGCTGTGGGGATTGC
NM_015324.3       AGGGATCTTCGCCAGCGGCCTGCATCCCTAGTGGTGGCTGACTTCGGCTGTGGGGATTGC
                  ************************************************************

HsCD00622824      CGCTTGGCTTCAAGTATCCGGAACCCTGTGCATTGCTTTGACTTGGCTTCTCTGGACCCT
NM_015324.3       CGCTTGGCTTCAAGTATCCGGAACCCTGTGCATTGCTTTGACTTGGCTTCTCTGGACCCT
                  ************************************************************

HsCD00622824      AGGGTCACTGTGTGTGACATGGCCCAGGTTCCTCTGGAGGATGAGTCTGTGGATGTGGCT
NM_015324.3       AGGGTCACTGTGTGTGACATGGCCCAGGTTCCTCTGGAGGATGAGTCTGTGGATGTGGCT
                  ************************************************************

HsCD00622824      GTGTTTTGCCTTTCACTGATGGGAACCAACATCAGGGACTTCCTAGAGGAGGCAAATAGA
NM_015324.3       GTGTTTTGCCTTTCACTGATGGGAACCAACATCAGGGACTTCCTAGAGGAGGCAAATAGA
                  ************************************************************

HsCD00622824      GTACTGAAGCCAGGGGGTCTCCTGAAAGTGGCTGAGGTCAGCAGCCGCTTTGAGGATGTT
NM_015324.3       GTACTGAAGCCAGGGGGTCTCCTGAAAGTGGCTGAGGTCAGCAGCCGCTTTGAGGATGTT
                  ************************************************************

HsCD00622824      CGAACCTTTCTGCGGGCTGTGACCAAGCTAGGCTTCAAGATTGTCTCCAAGGACCTGACC
NM_015324.3       CGAACCTTTCTGCGGGCTGTGACCAAGCTAGGCTTCAAGATTGTCTCCAAGGACCTGACC
                  ************************************************************

HsCD00622824      AACAGCCATTTCTTCTTGTTTGATTTCCAAAAGACTGGGCCCCCTCTGGTAGGGCCCAAG
NM_015324.3       AACAGCCATTTCTTCTTGTTTGATTTCCAAAAGACTGGGCCCCCTCTGGTAGGGCCCAAG
                  ************************************************************

HsCD00622824      GCTCAGCTTTCAGGCCTGCAGCTTCAGCCATGTCTCTACAAGCGCAGGTGA
NM_015324.3       GCTCAGCTTTCAGGCCTGCAGCTTCAGCCATGTCTCTACAAGCGCAGGTGA
                  ***************************************************

Pathways

Reactome :

Epigenetic regulation of gene expression

Reactome :

Gene Expression

Reactome :

Negative epigenetic regulation of rRNA expression

Reactome :

SIRT1 negatively regulates rRNA Expression

WikiPathway :

Epithelium TarBase

WikiPathway :

Lymphocyte TarBase

Protein

HPRD :

13800

Clone

Cloning Information :

671201

Original Clone ID :

ccsbBroadEn_02756

Organism

Species Specific ID:

23378

Genome

Chromosome :

Map Location :

11p15.4