Gene:
Gene Symbol:
RAD54L2
Gene Name:
RAD54-like 2 (S. cerevisiae)
Original Clone ID:
ccsbBroadEn_11682
Keyword:
PERFECT_MATCH
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00623009
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGAGAAGAAACATACGAAAGCTACTCCGGGAGGATCAATTGGAGCCTGTTACCAAAGCA
GCACAGCAAGAAGAGTTGGAAAGAAGGAAGCGCCTGGAGCAGCAGAGGAAAGATTATGCA
GCCCCTATTCCTACTGTTCCGCTGGAGTTCCTCCCTGAGGAAATTGCTTTAAGGGCAAGT
GACGGTCCCCAACTGCCTCCTCGGGTCTTGGCCCAGGAAGTCATTTGTTTGGACAGTAGC
AGTGGCAGTGAGGATGAAAAAAGCAGTCGAGATGAGGTGATTGAACTGAGCTCTGGAGAG
GAGGACACTCTGCACATTGTGGACAGCAGTGAATCTGTCAGTGAAGATGATGAGGAAGAA
GAGAAGGGTGGCACCCATGTCAATGATGTCTTAAACCAGCGTGACGCCCTTGGGCGGGTC
TTTGTCAACCTAAACCACCCTCCAGAGGAGGAAAATGTCTTCCTTGCCCCACAGTTGGCA
CGGGCTGTGAAACCTCATCAGATTGGCGGGATCCGGTTCCTTTACGATAACCTAGTGGAG
TCTCTGGAGAGGTTTAAGACCAGCAGTGGCTTTGGCTGTATTCTGGCCCACAGCATGGGT
CTGGGGAAAACTTTGCAAGTGATCTCTTTCATCGACGTCCTCTTCCGCCACACGCCAGCC
AAAACAGTCCTTGCCATTGTGCCGGTTAATACTCTTCAGAATTGGCTGGCAGAGTTCAAC
ATGTGGCTTCCACCTCCTGAAGCCCTCCCGGCTGACAACAAGCCTGAAGAAGTCCAGCCT
CGGTTCTTTAAAGTTCACATCTTGAATGATGAGCACAAGACGATGGCATCTCGTGCTAAA
GTGATGGCTGATTGGGTGTCAGAGGGTGGCGTGCTGCTGATGGGGTACGAGATGTACAGA
CTCCTCACTCTGAAGAAATCATTTGCCACAGGTAGACCGAAGAAAACCAAGAAGCGTTCT
CACCCAGTCATCATTGATCTAGATGAGGAAGATCGGCAGCAGGAGTTTCGGAGAGAGTTT
GAGAAGGCTTTATGCCGCCCTGGCCCTGATGTAGTAATCTGTGATGAGGGACACCGCATC
AAAAACTGCCAGGCCAGCACCTCACAGGCTCTGAAGAATATCCGCTCTCGCCGCCGGGTG
GTGCTGACTGGGTACCCTCTGCAAAACAACCTCATTGAGTACTGGTGCATGGTGGACTTT
GTGCGCCCAGACTTCCTTGGCACCCGGCAGGAGTTCAGCAACATGTTTGAACGCCCTATC
CTGAATGGGCAATGTATTGACAGCACACCTCAGGACGTCCGCCTCATGCGGTACCGGAGC
CATGTCCTGCACAGTCTTCTGGAGGGCTTTGTGCAGAGGAGAGGCCACACTGTGCTGAAG
ATTCATCTCCCTGCCAAGGAAGAAAATGTGATCCTTGTGCGGCTCTCCAAGATCCAGCGA
GATTTGTATACACAGTTCATGGATCGCTTCCGGGACTGTGGTAGCAGCGGTTGGCTGGGG
CTGAACCCCCTTAAGGCATTCTGTGTGTGTTGCAAGATCTGGAATCACCCTGATGTGCTG
TATGAAGCCCTTCAGAAGGAGAGCTTGGCCAATGAGCAGGACCTAGACGTGGAAGAACTT
GGCTCTGCAGGGACCAGTGCCCGCTGTCCACCACAGGGAACAAAAGGCAAGGGAGAGGAT
AGCACCTTGGCTTCCTCGATGGGAGAGGCAACCAATAGCAAGTTCCTACAGGGCGTTGGC
TTCAACCCTTTCCAGGAGCGAGGCAACAACATTGTCACATATGAATGGGCCAAGGACCTT
CTGACTAATTACCAGACTGGAGTCCTAGAAAACTCTCCCAAGATGGTACTGCTTTTCCAC
CTGATTGAGGAAAGTGTGAAGCTTGGGGACAAGATCCTTGTGTTTAGCCAGAGTCTTTCC
ACCTTGGCTCTCATCGAGGAATTCCTTGGAAAACGAGAAGTACCCTGTCCACCTGGTACC
GAGGGGCAAGGAGCACAGAAGTGGGTTCGAAACATCAGCTACTTCCGGCTAGATGGTAGC
ACCCCTGCCTTTGAGAGGGAGCGGCTTATTAATCAGTTCAATGATCCCAGCAACCTCACC
ACCTGGCTGTTCCTTCTCTCTACAAGGGCCGGATGCTTGGGTGTGAATCTGATTGGTGCC
AACCGAGTGGTGGTGTTTGATGCTTCCTGGAACCCTTGCCATGATGCCCAGGCAGTATGT
CGGGTATACCGTTATGGCCAGAAAAAGCCCTGTTACATCTATCGCCTTGTGGCTGATTAC
ACTCTAGAAAAGAAGATCTATGACCGTCAGATTTCCAAGCAGGGCATGTCAGATCGGGTG
GTGGATGATCTAAATCCAATGCTGAACTTCACACGGAAAGAGGTGGAAAACCTACTGCAC
TTTGTTGAGAAGGAGCCAGCTCCCCAAGTTTCCTTGAACGTAAAGGGGATCAAGGAGTCA
GTCCTGCAACTGGCCTGTCTGAAGTACCCTCACCTCATCACCAAGGAGCCTTTCGAGCAT
GAGTCATTGCTCTTGAACCGAAAGGATCACAAGCTAACCAAGGCTGAGAAAAAAGCAGCA
AAGAAAAGCTATGAGGAAGACAAACGCACATCAGTCCCCTATACCCGCCCATCGTATGCG
CAGTATTACCCTGCCAGCGATCAGAGCCTGACCAGCATCCCCGCCTTCAGCCAGAGAAAC
TGGCAGCCAACTTTGAAGGGTGATGAAAAGCCTGTGGCCAGTGTTCGTCCTGTGCAGTCC
ACCCCCATCCCCATGATGCCCCGGCATGTCCCATTGGGAGGAAGTGTAAGCTCTGCCTCC
AGCACAAATCCATCCATGAACTTTCCCATCAACTACTTGCAGCGTGCAGGAGTCCTTGTG
CAGAAGGTGGTCACCACGACAGATATTGTTATTCCTGGACTCAACAGCTCCACAGATGTA
CAGGCAAGAATTAATGCTGGTGAGAGCATCCACATCATCCGTGGGACAAAAGGGACGTAC
ATCCGTACCAGTGATGGACGGATCTTTGCTGTCCGGGCAACTGGCAAACCAAAGGTTCCT
GAAGATGGTCGGATGGCTGCCTCAGGTTCCCAGGGACCTTCTTGCGAGTCCACAAGCAAC
GGCAGACACAGTGCCTCATCACCCAAAGCCCCCGACCCTGAGGGGCTGGCCAGGCCCGTC
TCTCCTGACAGCCCAGAGATCATCAGTGAGCTTCAGCAGTATGCAGATGTGGCTGCTGCC
CGGGAATCCCGTCAGAGCTCCCCAAGCACCAATGCCGCCCTGCCTGGCCCCCCGGCCCAA
CTTATGGACAGCAGTGCTGTTCCCGGGACAGCTCTCGGAACTGAGCCTCGACTAGGGGGT
CATTGCCTCAATAGTTCCCTCTTGGTGACTGGCCAGCCCTGTGGTGACAGGCACCCAGTG
CTGGACTTAAGGGGCCACAAGCGAAAGTTGGCCACACCACCTGCTGCCCAGGAGTCATCC
CGCCGGCGGTCCAGGAAGGGTCATCTGCCAGCCCCCGTGCAGCCGTATGAACACGGGTAT
CCAGTCTCTGGCGGGTTTGCCATGCCACCCGTCTCCTTAAACCATAACCTCACCACCCCC
TTCACCTCCCAGGCTGGGGAGAACTCCCTGTTTATGGGCAGTACCCCCTCCTACTACCAG
CTGTCCAATTTGCTGGCAGATGCCCGCCTGGTGTTTCCAGTGACTACTGACCCTCTGGTG
CCAGCAGGCCCCGTCAGTTCCTCTTCCACGGCTACCTCAGTCACTGCCAGCAACCCCTCC
TTCATGCTCAACCCTTCTGTGCCAGGGATACTACCCAGCTATTCACTCCCATTCTCACAG
CCACTCCTGTCCGAGCCGAGGATGTTTGCGCCTTTTCCTTCCCCTGTCTTGCCCAGCAAC
CTTTCGCGGGGCATGTCTATCTATCCAGGCTACATGTCCCCACATGCAGGCTACCCAGCT
GGTGGCCTCCTACGGTCCCAGGTGCCTCCATTTGACTCTCATGAGGTTGCCGAGGTTGGG
TTCAGCTCCAATGATGATGAGGATAAAGACGATGATGTGATAGAGGTCACTGGGAAATAG
Protein sequence predicted based on nucleotide sequence
MRRNIRKLLREDQLEPVTKAAQQEELERRKRLEQQRKDYAAPIPTVPLEFLPEEIALRAS DGPQLPPRVLAQEVICLDSSSGSEDEKSSRDEVIELSSGEEDTLHIVDSSESVSEDDEEE EKGGTHVNDVLNQRDALGRVFVNLNHPPEEENVFLAPQLARAVKPHQIGGIRFLYDNLVE SLERFKTSSGFGCILAHSMGLGKTLQVISFIDVLFRHTPAKTVLAIVPVNTLQNWLAEFN MWLPPPEALPADNKPEEVQPRFFKVHILNDEHKTMASRAKVMADWVSEGGVLLMGYEMYR LLTLKKSFATGRPKKTKKRSHPVIIDLDEEDRQQEFRREFEKALCRPGPDVVICDEGHRI KNCQASTSQALKNIRSRRRVVLTGYPLQNNLIEYWCMVDFVRPDFLGTRQEFSNMFERPI LNGQCIDSTPQDVRLMRYRSHVLHSLLEGFVQRRGHTVLKIHLPAKEENVILVRLSKIQR DLYTQFMDRFRDCGSSGWLGLNPLKAFCVCCKIWNHPDVLYEALQKESLANEQDLDVEEL GSAGTSARCPPQGTKGKGEDSTLASSMGEATNSKFLQGVGFNPFQERGNNIVTYEWAKDL LTNYQTGVLENSPKMVLLFHLIEESVKLGDKILVFSQSLSTLALIEEFLGKREVPCPPGT EGQGAQKWVRNISYFRLDGSTPAFERERLINQFNDPSNLTTWLFLLSTRAGCLGVNLIGA NRVVVFDASWNPCHDAQAVCRVYRYGQKKPCYIYRLVADYTLEKKIYDRQISKQGMSDRV VDDLNPMLNFTRKEVENLLHFVEKEPAPQVSLNVKGIKESVLQLACLKYPHLITKEPFEH ESLLLNRKDHKLTKAEKKAAKKSYEEDKRTSVPYTRPSYAQYYPASDQSLTSIPAFSQRN WQPTLKGDEKPVASVRPVQSTPIPMMPRHVPLGGSVSSASSTNPSMNFPINYLQRAGVLV QKVVTTTDIVIPGLNSSTDVQARINAGESIHIIRGTKGTYIRTSDGRIFAVRATGKPKVP EDGRMAASGSQGPSCESTSNGRHSASSPKAPDPEGLARPVSPDSPEIISELQQYADVAAA RESRQSSPSTNAALPGPPAQLMDSSAVPGTALGTEPRLGGHCLNSSLLVTGQPCGDRHPV LDLRGHKRKLATPPAAQESSRRRSRKGHLPAPVQPYEHGYPVSGGFAMPPVSLNHNLTTP FTSQAGENSLFMGSTPSYYQLSNLLADARLVFPVTTDPLVPAGPVSSSSTATSVTASNPS FMLNPSVPGILPSYSLPFSQPLLSEPRMFAPFPSPVLPSNLSRGMSIYPGYMSPHAGYPA GGLLRSQVPPFDSHEVAEVGFSSNDDEDKDDDVIEVTGK*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223
Synonyms:
pDONR223, pENTR223-XnCDSYn
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp):
2815
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Mark Vidal
Publications:
PMID:
15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
bacterial origin of replication
2220
2753
gene fragment
pas
5p stem of pBR primose assembly site
63
73
gene fragment
pas prime
3p stem of pBR pas(L)
105
116
recombination site
attL1
attL1 recombination site
570
665
recombination site
attL2
attL2 recombination site
793
696
selectable marker
SpnR
spectinomycin resistance gene
1062
2072
trxn termination sequence
T1-T2
rrnBT1 and T2 terminators
510
224
