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Detailed Vector Information: pJSP6_cHalo_DC

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Explanation of Terms


Gene: Gene Symbol:  No insert
Gene Name:  No insert
Original Clone ID: None
Type: No insert
Vector Name: pJSP6_cHalo_DC              
Source: Center for Personalized Diagnostics
Description: Wheat germ expression vector with SP6 promoter and TEV-cleavable C-terminal HALO tag; ampicillin resistance in bacteria; gateway cloning
Comments: None
Publications: None
Authors: Joshua LaBaer
Center for Personalized Diagnostics
Arizona State University
Ji Qiu
Justin Saul
EvNO00623724

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AVAILABLE
No restriction
Special MTA: HALO

 

 

 Recommended Growth Condition:

Distributed in bacterial strain : DB3.1 T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
       DyNA Vector Map

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Vector Name: pJSP6_cHalo_DC

pJSP6_cHalo_DC in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  SP6
Reverse:  cHaloseqR
Description: Wheat germ expression vector with SP6 promoter and TEV-cleavable C-terminal HALO tag; ampicillin resistance in bacteria; gateway cloning
Comments: None
Size (bp): 6502
Parent Vector: None
Properties: Gateway, acceptor (destination), cell-free expression, recombinational cloning, with tag/fusion/marker
Author Name: Joshua LaBaer
Center for Personalized Diagnostics
Arizona State University
Ji Qiu
Justin Saul
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 bacterial origin 3956 4638
death cassette ccdB ccdB death cassette (removed in plasmids containing a gene insert) 1471 1680
enhancer TMV Omega TMV Omega enhancer 9 129
protease cleavage site TEV TEV protease cleavage site 1871 1891
recombination site attR1 attR1 recombination site 144 160
recombination site attR2 attR2 recombinational cloning site 1831 1846
selectable marker AmpR ampicillin resistance 4736 5395
selectable marker CmR Chloramphenicol resistance (removed in plasmids containing a gene insert) 377 1033

 

Species Specific ID: None
Target GenBank: None