Detailed Plasmid Information

Explanation of Terms

Gene:
Gene Symbol:  PA0975
Gene Name:  radical activating enzyme
Original Clone ID:
FLH033948.01F
Keyword:
None
Species:
Pseudomonas aeruginosa
Type:
cDNA
Vector Name:
pDONR201
Format: 
FUSION
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
HIP
PaCD00006447
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 795nts
Open reading frame: 1 to 795

ATGGTTTCAGCTCGGCTGATCTTCGTGCCTCTCCACGCGAGACCTTCCGGGATTTCGAGC
GCCACGACAGGATACGTCCCGTGGCGCTCCTTCATTTACCGCAACGGAGGCGGACGACCT
GTTCAGTCGTCACGCCCGTGGCTGAATATGCAACAGACCCTGCGCATCACCGAGATTTTC
TACTCGTTGCAGGGGGAAACACGTACCGCCGGCTTGCCGACGGTCTTCGTGCGCCTGACC
GGCTGCCCCTTGCGCTGTCACTATTGCGATACCGCCTATGCCTTCAGCGGCGGCGACGTC
GTTAGCCTGGACGCCATTTTCGAGCGGGTTGCCGCCTACAAGCCCCGCTACATCTGCGTG
ACCGGCGGGGAGCCGCTGGCCCAGCCGAATTGCATCTCTCTGCTGGAACGGCTCTGCGAC
GCCGGCTACGAAGTTTCCCTGGAAACCAGCGGGGCTCTCGACGTGTCACGGGTCGACCCG
CGCGTAAGCAAGGTTCTCGACCTCAAGACGCCGGGATCTGGCGAGGTCGGACGCAACCGA
TACGAAAATATCCCGTTGCTGACCGACAACGATCAGGTGAAGTTCGTCGTCTGTTCGCGA
GAGGACTACGACTGGGCGGTATCCAAGCTGATCGAGTACCGCCTCGACCAGCGTGCCGGT
GAAGTCCTGTTCTCGCCGAGCCACCATCAGGTCAGCGCGCGAGAACTGGCCGATTGGATC
GTGGCCGACAACCTGCCGGTCCGCCTGCAATTGCAACTGCACAAGATTCTCTGGAACGAC
GAACCGGGCCATGGA

Protein sequence predicted based on nucleotide sequence

MVSARLIFVPLHARPSGISSATTGYVPWRSFIYRNGGGRPVQSSRPWLNMQQTLRITEIF
YSLQGETRTAGLPTVFVRLTGCPLRCHYCDTAYAFSGGDVVSLDAIFERVAAYKPRYICV
TGGEPLAQPNCISLLERLCDAGYEVSLETSGALDVSRVDPRVSKVLDLKTPGSGEVGRNR
YENIPLLTDNDQVKFVVCSREDYDWAVSKLIEYRLDQRAGEVLFSPSHHQVSARELADWI
VADNLPVRLQLQLHKILWNDEPGHG

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 795
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p ACAAGTTTGTACAAAAAAGCAGGCTCCGAAGGAGATACC(atg-goi)
3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTACAAAGTGGT

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable