DNASU Plasmid Repository • 480.965.5697 | Email

GUK1 (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

TOP

Explanation of Terms


Gene: Gene Symbol:  GUK1
Gene Name:  guanylate kinase 1
Sequence              Map: pDNR-Dual_with_human_insert.pdf
Original Clone ID: FLH008045.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDNR-Dual               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: PMID: 15928075
Title: Building a human kinase gene repository: bioinformatics, molecular cloning, and functional validation.
Authors: HIP
HsCD00000651

Price:  Login for Pricing
AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 594nts         Open reading frame : 1 to 594
Reference Sequence Alignment

ATGTCGGGCCCCAGGCCTGTGGTGCTGAGCGGGCCTTCGGGAGCTGGGAAGAGCACCCTG
CTGAAGAGGCTGCTCCAGGAGCACAGCGGCATCTTTGGCTTCAGCGTGTCCCATACCACG
AGGAACCCGAGGCCCGGCGAGGAGAACGGCAAAGATTACTACTTTGTAACCAGGGAGGTG
ATGCAGCGTGACATAGCAGCCGGCGACTTCATCGAGCATGCCGAGTTCTCGGGGAACCTG
TATGGCACGAGCAAGGTGGCGGTGCAGGCCGTGCAGGCCATGAACCGCATCTGTGTGCTG
GACGTGGACCTGCAGGGTGTGCGGAACATCAAGGCCACCGATCTGCGGCCCATCTACATC
TCTGTGCAGCCGCCTTCACTGCACGTGCTGGAGCAGCGGCTGCGGCAGCGCAACACTGAA
ACCGAGGAGAGCCTGGTGAAGCGGCTGGCTGCTGCCCAGGCCGACATGGAGAGCAGCAAG
GAGCCCGGCCTGTTTGATGTGGTCATCATTAACGACAGCCTGGACCAGGCCTACGCAGAG
CTGAAGGAGGCGCTCTCTGAGGAAATCAAGAAAGCTCAAAGGACCGGCGCCTTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 594
Start on reference sequence 1
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pDNR-Dual

pDNR-Dual in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  Unknown
Description: Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from).
Comments: The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp): 4938
Parent Vector: None
Empty Vector: None
Properties: Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name: BD Biosciences Clontech
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS restriction enzyme-type multiple cloning site 45 129
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4111 4574
negative selection marker SacB SacB negative selection marker gene (sucrose sensitivity) 1057 2972
primer site M13 primer M13 primer sequencing site 4839 4855
primer site T7 primer T7 RNA Pol primer sequencing site 4865 4883
recombination site loxP site loxP recombination site 1015 1048
recombination site loxP site loxP recombination site 9 42
selectable marker CmR chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) 986 327
selectable marker AmpR ampicillin resistance gene 3103 3963
splice donor site SD site splice donor site 132 136
tag HN 6xHN epitope tag 141 176

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : GUK1
Symbol Nomenclature : GUK1
SYNONYM : GMK
Designation : ATP:GMP phosphotransferase|GMP kinase|guanylate kinase
Full Nomenclature : guanylate kinase 1
GENEID : 2987
Locus Tag : RP11-520H14.2
GI : 61366517
GenBank Accession : AY890933
HGNC : 4693
MIM : 139270
Vega : OTTHUMG00000039503
Target GenBank: NM_000858

 

Reference Sequence Alignment

  

HsCD00000651        ------------------------------------------------------------
NM_001242840.1      ATGCTGCGGCGCCCGCTGGCCGGGCTGGCTGCGGCCGCCCTGGGCCGGGCCCCACCGGAC
NM_001159391.1      ------------------------------------------------------------
NM_001242839.1      ------------------------------------------------------------
NM_001159390.1      ATGCTGCGGCGCCCGCTGGCCGGGCTGGCTGCGGCCGCCCTGGGCCGGGCCCCACCGGAC
NM_000858.5         ------------------------------------------------------------
XM_005273106.1      ------------------------------------------------------------
                                                                                

HsCD00000651        ---ATGTCGGGCCCCAGGCCTGTGGTGCTGAGCGGGCCTTCGGGAGCTGGGAAGAGCACC
NM_001242840.1      GGCATGTCGGGCCCCAGGCCTGTGGTGCTGAGCGGGCCTTCGGGAGCTGGGAAGAGCACC
NM_001159391.1      ---ATGTCGGGCCCCAGGCCTGTGGTGCTGAGCGGGCCTTCGGGAGCTGGGAAGAGCACC
NM_001242839.1      ---ATGTCGGGCCCCAGGCCTGTGGTGCTGAGCGGGCCTTCGGGAGCTGGGAAGAGCACC
NM_001159390.1      GGCATGTCGGGCCCCAGGCCTGTGGTGCTGAGCGGGCCTTCGGGAGCTGGGAAGAGCACC
NM_000858.5         ---ATGTCGGGCCCCAGGCCTGTGGTGCTGAGCGGGCCTTCGGGAGCTGGGAAGAGCACC
XM_005273106.1      ---ATGTCGGGCCCCAGGCCTGTGGTGCTGAGCGGGCCTTCGGGAGCTGGGAAGAGCACC
                       *********************************************************

HsCD00000651        CTGCTGAAGAGGCTGCTCCAGGAGCACAGCGGCATCTTTGGCTTCAGCGTGTCCCATACC
NM_001242840.1      CTGCTGAAGAGGCTGCTCCAGGAGCACAGCGGCATCTTTGGCTTCAGCGTGTCCCATACC
NM_001159391.1      CTGCTGAAGAGGCTGCTCCAGGAGCACAGCGGCATCTTTGGCTTCAGCGTGTCCCATACC
NM_001242839.1      CTGCTGAAGAGGCTGCTCCAGGAGCACAGCGGCATCTTTGGCTTCAGCGTGTCCCATACC
NM_001159390.1      CTGCTGAAGAGGCTGCTCCAGGAGCACAGCGGCATCTTTGGCTTCAGCGTGTCCCATACC
NM_000858.5         CTGCTGAAGAGGCTGCTCCAGGAGCACAGCGGCATCTTTGGCTTCAGCGTGTCCCATACC
XM_005273106.1      CTGCTGAAGAGGCTGCTCCAGGAGCACAGCGGCATCTTTGGCTTCAGCGTGTCCCATACC
                    ************************************************************

HsCD00000651        ACGAGGAACCCGAGGCCCGGCGAGGAGAACGGCAAAGATTACTACTTTGTAACCAGGGAG
NM_001242840.1      ACGAGGAACCCGAGGCCCGGCGAGGAGAACGGCAAAGATTACTACTTTGTAACCAGGGAG
NM_001159391.1      ACGAGGAACCCGAGGCCCGGCGAGGAGAACGGCAAAGATTACTACTTTGTAACCAGGGAG
NM_001242839.1      ACGAGGAACCCGAGGCCCGGCGAGGAGAACGGCAAAGATTACTACTTTGTAACCAGGGAG
NM_001159390.1      ACGAGGAACCCGAGGCCCGGCGAGGAGAACGGCAAAGATTACTACTTTGTAACCAGGGAG
NM_000858.5         ACGAGGAACCCGAGGCCCGGCGAGGAGAACGGCAAAGATTACTACTTTGTAACCAGGGAG
XM_005273106.1      ACGAGGAACCCGAGGCCCGGCGAGGAGAACGGCAAAGATTACTACTTTGTAACCAGGGAG
                    ************************************************************

HsCD00000651        GTGATGCAGCGTGACATAGCAGCCGGCGACTTCATCGAGCATGCCGAGTTCTCGGGGAAC
NM_001242840.1      GTGATGCAGCGTGACATAGCAGCCGGCGACTTCATCGAGCATGCCGAGTTCTCGGGGAAC
NM_001159391.1      GTGATGCAGCGTGACATAGCAGCCGGCGACTTCATCGAGCATGCCGAGTTCTCGGGGAAC
NM_001242839.1      GTGATGCAGCGTGACATAGCAGCCGGCGACTTCATCGAGCATGCCGAGTTCTCGGGGAAC
NM_001159390.1      GTGATGCAGCGTGACATAGCAGCCGGCGACTTCATCGAGCATGCCGAGTTCTCGGGGAAC
NM_000858.5         GTGATGCAGCGTGACATAGCAGCCGGCGACTTCATCGAGCATGCCGAGTTCTCGGGGAAC
XM_005273106.1      GTGATGCAGCGTGACATAGCAGCCGGCGACTTCATCGAGCATGCCGAGTTCTCGGGGAAC
                    ************************************************************

HsCD00000651        CTGTATGGCACGAGCAAGGTGGCGGTGCAGGCCGTGCAGGCCATGAACCGCATCTGTGTG
NM_001242840.1      CTGTATGGCACGAGCAAGGTGGCGGTGCAGGCCGTGCAGGCCATGAACCGCATCTGTGTG
NM_001159391.1      CTGTATGGCACGAGCAAGGTGGCGGTGCAGGCCGTGCAGGCCATGAACCGCATCTGTGTG
NM_001242839.1      CTGTATGGCACGAGCAAGGTGGCGGTGCAGGCCGTGCAGGCCATGAACCGCATCTGTGTG
NM_001159390.1      CTGTATGGCACGAGCAAGGTGGCGGTGCAGGCCGTGCAGGCCATGAACCGCATCTGTGTG
NM_000858.5         CTGTATGGCACGAGCAAGGTGGCGGTGCAGGCCGTGCAGGCCATGAACCGCATCTGTGTG
XM_005273106.1      CTGTATGGCACGAGCAAGGTGGCGGTGCAGGCCGTGCAGGCCATGAACCGCATCTGTGTG
                    ************************************************************

HsCD00000651        CTGGACGTGGACCTGCAGGGTGTGCGGAACATCAAGGCCACCGATCTGCGGCCCATCTAC
NM_001242840.1      CTGGACGTGGACCTGCAGGGTGTGCGGAACATCAAGGCCACCGATCTGCGGCCCATCTAC
NM_001159391.1      CTGGACGTGGACCTGCAGGGTGTGCGGAACATCAAGGCCACCGATCTGCGGCCCATCTAC
NM_001242839.1      CTGGACGTGGACCTGCAGGGTGTGCGGAACATCAAGGCCACCGATCTGCGGCCCATCTAC
NM_001159390.1      CTGGACGTGGACCTGCAGGGTGTGCGGAACATCAAGGCCACCGATCTGCGGCCCATCTAC
NM_000858.5         CTGGACGTGGACCTGCAGGGTGTGCGGAACATCAAGGCCACCGATCTGCGGCCCATCTAC
XM_005273106.1      CTGGACGTGGACCTGCAGGGTGTGCGGAACATCAAGGCCACCGATCTGCGGCCCATCTAC
                    ************************************************************

HsCD00000651        ATCTCTGTGCAGCCGCCTTCACTGCACGTGCTGGAGCAGCGGCTGCGGCAGCGCAACACT
NM_001242840.1      ATCTCTGTGCAGCCGCCTTCACTGCACGTGCTGGAGCAGCGGCTGCGGCAGCGCAACACT
NM_001159391.1      ATCTCTGTGCAGCCGCCTTCACTGCACGTGCTGGAGCAGCGGCTGCGGCAGCGCAACACT
NM_001242839.1      ATCTCTGTGCAGCCGCCTTCACTGCACGTGCTGGAGCAGCGGCTGCGGCAGCGCAACACT
NM_001159390.1      ATCTCTGTGCAGCCGCCTTCACTGCACGTGCTGGAGCAGCGGCTGCGGCAGCGCAACACT
NM_000858.5         ATCTCTGTGCAGCCGCCTTCACTGCACGTGCTGGAGCAGCGGCTGCGGCAGCGCAACACT
XM_005273106.1      ATCTCTGTGCAGCCGCCTTCACTGCACGTGCTGGAGCAGCGGCTGCGGCAGCGCAACACT
                    ************************************************************

HsCD00000651        GAAACCGAGGAGAGCCTGGTGAAGCGGCTGGCTGCTGCCCAGGCCGACATGGAGAGCAGC
NM_001242840.1      GAAACCGAGGAGAGCCTGGTGAAGCGGCTGGCTGCTGCCCAGGCCGACATGGAGAGCAGA
NM_001159391.1      GAAACCGAGGAGAGCCTGGTGAAGCGGCTGGCTGCTGCCCAGGCCGACATGGAGAGCAGC
NM_001242839.1      GAAACCGAGGAGAGCCTGGTGAAGCGGCTGGCTGCTGCCCAGGCCGACATGGAGAGCAGC
NM_001159390.1      GAAACCGAGGAGAGCCTGGTGAAGCGGCTGGCTGCTGCCCAGGCCGACATGGAGAGCAGC
NM_000858.5         GAAACCGAGGAGAGCCTGGTGAAGCGGCTGGCTGCTGCCCAGGCCGACATGGAGAGCAGC
XM_005273106.1      GAAACCGAGGAGAGCCTGGTGAAGCGGCTGGCTGCTGCCCAGGCCGACATGGAGAGCAGA
                    ***********************************************************.

HsCD00000651        AAGGAGCCCGGCCTGTTTGATG--------------------------------------
NM_001242840.1      AATCAAGAAAGCTCAAAGGACCGGCGCCTGAGGCTTGCTGTCTGTTCTCGGCACCCCGGG
NM_001159391.1      AAGGAGCCCGGCCTGTTTGATG--------------------------------------
NM_001242839.1      AAGGAGCCCGGCCTGTTTGATG--------------------------------------
NM_001159390.1      AAGGAGCCCGGCCTGTTTGATG--------------------------------------
NM_000858.5         AAGGAGCCCGGCCTGTTTGATG--------------------------------------
XM_005273106.1      AATCAAGAAAGCTCAAAGGACCGGCGCCTGAGGCTTGCTGTCTGTTCTCGGCACCCCGGG
                    **  *. ...**  .:: **                                        

HsCD00000651        ----TGGTCATCATTAACGACAGCCTGGACCAGGCCTACGCAGAGCTGAAGGAGGCGCTC
NM_001242840.1      CCCATACAGGACCAGGGCAGCAGCATTGAGCCACCCCCTTGGCAGGCGATACGGCAGCTC
NM_001159391.1      ----TGGTCATCATTAACGACAGCCTGGACCAGGCCTACGCAGAGCTGAAGGAGGCGCTC
NM_001242839.1      ----TGGTCATCATTAACGACAGCCTGGACCAGGCCTACGCAGAGCTGAAGGAGGCGCTC
NM_001159390.1      ----TGGTCATCATTAACGACAGCCTGGACCAGGCCTACGCAGAGCTGAAGGAGGCGCTC
NM_000858.5         ----TGGTCATCATTAACGACAGCCTGGACCAGGCCTACGCAGAGCTGAAGGAGGCGCTC
XM_005273106.1      CCCATACAGGACCAGGGCAGCAGCATTGAGCCACCCCCTTGGCAGGCGATACGGCAGCTC
                        *. : .:*.: ..*..****.* ** *.. ** .   . **  **:. .* .****

HsCD00000651        TCTGAGGAAATCAAGAAAGCTCAAAGGACCGGCGCCTTG---------------------
NM_001242840.1      TGTGCCCTTGGCCAGCATGTGGAGTGGAGGAGATGCTGCCCCTGTGGTTGGAACATCCTG
NM_001159391.1      TCTGAGGAAATCAAGAAAGCTCAAAGGACCGGCGCCTGA---------------------
NM_001242839.1      TCTGAGGAAATCAAGAAAGCTCAAAGGACCGGCGCCTGA---------------------
NM_001159390.1      TCTGAGGAAATCAAGAAAGCTCAAAGGACCGGCGCCTGA---------------------
NM_000858.5         TCTGAGGAAATCAAGAAAGCTCAAAGGACCGGCGCCTGA---------------------
XM_005273106.1      TGTGCCCTTGGCCAGCATGTGGAGTGGAGGAGATGCTGCCCCTGTGGTTGGAACATCCTG
                    * **.  ::. *.**.*:*   *.:***  .*.  **                       

HsCD00000651        ------
NM_001242840.1      GGGTGA
NM_001159391.1      ------
NM_001242839.1      ------
NM_001159390.1      ------
NM_000858.5         ------
XM_005273106.1      GGGTGA
                          

Pathways

Panther : De novo purine biosynthesis
Reactome : Abacavir metabolism
Reactome : Abacavir transport and metabolism
Reactome : Metabolism
Reactome : Metabolism of nucleotides
Reactome : Synthesis and interconversion of nucleotide di- and triphosphates

Protein

SMART domain : GuKc : Guanylate kinase homologues.
UniProt : Q16774
UniProt : B4E1H6
UniProt : Q6IBG8
HPRD : 00755

Clone

Cloning Information : 8045
HIP Master Clone ID : 7603
Original Clone ID : FLH008045.01L

Organism


TAX_ID : 9606
Species Specific ID: 2987

Genome

Chromosome : 1
Map Location : 1q32-q41
Ensembl : ENSG00000143774

Reagents

Labome : GUK1-antibody