NANOG (Homo sapiens) in pET15Nano6HT_NESG

Explanation of Terms

Gene:
Gene Symbol:  NANOG
Gene Name:  Nanog homeobox
Original Clone ID:
HR6963A-75-157-TEV.2
Keyword:
targeted domain
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pET15Nano6HT_NESG
Format: 
CLOSED
Source:
Northeast Structural Genomics Consortium
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
Rutgers University
PSI:Biology
Northeast Structural Genomics Consortium
HsCD00651327
Price: 
Login for Pricing
AVAILABLE
No restriction
Special MTA: PSI/NESG
Insert sequence: 252nts
Open reading frame: 10 to 261

AGCCATATGAAACAACCCACTTCTGCAGAGAATAGTGTCGCAAAAAAGGAAGACAAGGTC
CCGGTCAAGAAACAGAAGACCAGAACTGTGTTCTCTTCCACCCAGCTGTGTGTACTCAAT
GATAGATTTCAGAGACAGAAATACCTCAGCCTCCAGCAGATGCAAGAACTCTCCAACATC
CTGAACCTCAGCTACAAACAGGTGAAGACCTGGTTCCAGAACCAGAGAATGAAATCTAAG
AGGTGGCAGAAAAACAACTAG

Protein sequence predicted based on nucleotide sequence

KQPTSAENSVAKKEDKVPVKKQKTRTVFSSTQLCVLNDRFQRQKYLSLQQMQELSNILNL
SYKQVKTWFQNQRMKSKRWQKNN*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
g24t,K8N
                                                    

5' Linker Sequence:
ATGGACGTTGAAGCTTGGCTGGACGAACGTGTTCCGCTGGTTGAAACCCA TCACCATCACCATCACGAAAACCTGTATTTTCAGAGCCATATG
3' Linker Sequence:
CTCGAGATCCGG

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinPurified: Not_Tested
SolubleProtein: Not_Tested
ProteinExpressed: Tested_Not_Found
Find experimental protocols in HR6963A
Recommended expression in:
Not Applicable