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RXRA (Homo sapiens) in pET15Avi6HT_NESG

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Explanation of Terms


Gene: Gene Symbol:  RXRA
Gene Name:  retinoid X receptor, alpha
Sequence             
Original Clone ID: None
Keyword: targeted domain
Species: Homo sapiens
Type: cDNA
Vector Name: pET15Avi6HT_NESG               Format:  FUSION
Source: Rutgers University
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: Stephen Anderson
Elliot Campbell
Rutgers University
Protein Capture Reagents Program
HsCD00651870

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AVAILABLE
No restriction
Special MTA: SPTA

 

Insert sequence: 588nts         Open reading frame : 1 to 588

GTCACCAACATTTGCCAAGCAGCCGACAAACAGCTTTTCACCCTGGTGGAGTGGGCCAAG
CGGATCCCACACTTCTCAGAGCTGCCCCTGGACGACCAGGTCATCCTGCTGCGGGCAGGC
TGGAATGAGCTGCTCATCGCCTCCTTCTCCCACCGCTCCATCGCCGTGAAGGACGGGATC
CTCCTGGCCACCGGGCTGCACGTCCACCGGAACAGCGCCCACAGCGCAGGGGTGGGCGCC
ATCTTTGACAGGGTGCTGACGGAGCTTGTGTCCAAGATGCGGGACATGCAGATGGACAAG
ACGGAGCTGGGCTGCCTGCGCGCCATCGTCCTCTTTAACCCTGACTCCAAGGGGCTCTCG
AACCCGGCCGAGGTGGAGGCGCTGAGGGAGAAGGTCTATGCGTCCTTGGAGGCCTACTGC
AAGCACAAGTACCCAGAGCAGCCGGGAAGGTTCGCTAAGCTCTTGCTCCGCCTGCCGGCT
CTGCGCTCCATCGGGCTCAAATGCCTGGAACATCTCTTCTTCTTCAAGCTCATCGGGGAC
ACACCCATTGACACCTTCCTTATGGAGATGCTGGAGGCGCCGCACTGA


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pET15Avi6HT_NESG

pET15Avi6HT_NESG in advanced viewed

Synonyms: ''
Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Bacterial expression vector with T7 lac promoter, N-terminal TEV-cleavable Avi-tag and 6XHis-tag; ampicillin resistance in bacteria; restriction enzyme cloning
Comments: None
Size (bp): 5789
Parent Vector: None
Empty Vector: None
Properties: bacterial expression, multiple cloning site, with tag/fusion/marker
Author Name: Rutgers University
Gaetano T. Montleione
Thomas B. Acton
Stephen Anderson
Northeast Structural Genomics Consortium
PSI:Biology
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 origin 3944 4626
promoter T7 T7 promoter 541 560
protease cleavage site TEV TEV protease cleavage site 387 404
repressor binding site lac operator Lac operator 519 541
repressor protein gene LacI Lac repressor 3344 3520
selectable marker AmpR ampicillin resistance 4724 5383
tag His N-terminal 6xHis tag 405 422
tag AviTag Avi Tag 423 466
trxn termination sequence T7 term T7 terminator 217 263

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Experimental
  • Organism
  • Genome

Gene

 

Gene Symbol : RXRA
Symbol Nomenclature : RXRA
SYNONYM : NR2B1
Designation : nuclear receptor subfamily 2 group B member 1|retinoic acid receptor RXR-alpha|retinoid X nuclear receptor alpha
Full Nomenclature : retinoid X receptor, alpha
GENEID : 6256
HGNC : 10477
MIM : 180245
Target GenBank: None

 

Pathways

NCI : a6b1 and a6b4 Integrin signaling
NCI : Regulation of Androgen receptor activity
NCI : Retinoic acid receptors-mediated signaling
NCI : RXR and RAR heterodimerization with other nuclear receptor
Panther : Vitamin D metabolism and pathway
Reactome : Activation of gene expression by SREBF (SREBP)
Reactome : BMAL1:CLOCK,NPAS2 activates circadian gene expression
Reactome : Circadian Clock
Reactome : Developmental Biology
Reactome : Fatty acid, triacylglycerol, and ketone body metabolism
Reactome : Gene Expression
Reactome : Generic Transcription Pathway
Reactome : Metabolism
Reactome : Metabolism of lipids and lipoproteins
Reactome : Mitochondrial biogenesis
Reactome : Nuclear Receptor transcription pathway
Reactome : Organelle biogenesis and maintenance
Reactome : PPARA activates gene expression
Reactome : Regulation of cholesterol biosynthesis by SREBP (SREBF)
Reactome : Regulation of lipid metabolism by Peroxisome proliferator-activated receptor alpha (PPARalpha)
Reactome : REV-ERBA represses gene expression
Reactome : RORA activates circadian gene expression
Reactome : Transcriptional activation of mitochondrial biogenesis
Reactome : Transcriptional regulation of white adipocyte differentiation
Reactome : YAP1- and WWTR1 (TAZ)-stimulated gene expression
WikiPathway : Adipogenesis
WikiPathway : Energy Metabolism
WikiPathway : Nuclear Receptors
WikiPathway : Vitamin A and carotenoid metabolism
WikiPathway : Vitamin D synthesis

Protein

Structural Annotation Wiki : HR4643C
UniProt : P19793
HPRD : 01577

Clone

Cloning Information : 678431

Experimental

TargetTrack - Experimental data : HR4643C

Organism


TAX_ID : 9606
Species Specific ID: 6256

Genome

Chromosome : 9
Map Location : 9q34.3