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ESR1 (Homo sapiens) in pET15Avi6HT_NESG

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Explanation of Terms


Gene: Gene Symbol:  ESR1
Gene Name:  estrogen receptor 1
Sequence             
Original Clone ID: None
Keyword: targeted domain
Species: Homo sapiens
Type: cDNA
Vector Name: pET15Avi6HT_NESG               Format:  FUSION
Source: Rutgers University
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: Stephen Anderson
Elliot Campbell
Rutgers University
Protein Capture Reagents Program
HsCD00651886

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AVAILABLE
No restriction
Special MTA: SPTA

 

Insert sequence: 645nts        
Open reading frame : 1 to 645

TTCAGTGAAGCTTCGATGATGGGCTTACTGACCAACCTGGCAGACAGGGAGCTGGTTCAC
ATGATCAACTGGGCGAAGAGGGTGCCAGGCTTTGTGGATTTGACCCTCCATGATCAGGTC
CACCTTCTAGAATGTGCCTGGCTAGAGATCCTGATGATTGGTCTCGTCTGGCGCTCCATG
GAGCACCCAGGGAAGCTACTGTTTGCTCCTAACTTGCTCTTGGACAGGAACCAGGGAAAA
TGTGTAGAGGGCATGGTGGAGATCTTCGACATGCTGCTGGCTACATCATCTCGGTTCCGC
ATGATGAATCTGCAGGGAGAGGAGTTTGTGTGCCTCAAATCTATTATTTTGCTTAATTCT
GGAGTGTACACATTTCTGTCCAGCACCCTGAAGTCTCTGGAAGAGAAGGACCATATCCAC
CGAGTCCTGGACAAGATCACAGACACTTTGATCCACCTGATGGCCAAGGCAGGCCTGACC
CTGCAGCAGCAGCACCAGCGGCTGGCCCAGCTCCTCCTCATCCTCTCCCACATCAGGCAC
ATGAGTAACAAAGGCATGGAGCATCTGTACAGCATGAAGTGCAAGAACGTGGTGCCCCTC
TATGACCTGCTGCTGGAGATGCTGGACGCCCACCGCCTACATTGA


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
ATGTCTGGTCTGAACGACATCTTCGAAGCTCAGAAAATCGAATGGCACGA
ACATCACCATCACCATCACGAAAACCTGTATTTTCAGAGCCATATG

3' Linker Sequence:
CTCGAGATCCGG

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pET15Avi6HT_NESG

pET15Avi6HT_NESG in advanced viewed

Synonyms: ''
Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Bacterial expression vector with T7 lac promoter, N-terminal TEV-cleavable Avi-tag and 6XHis-tag; ampicillin resistance in bacteria; restriction enzyme cloning
Comments: None
Size (bp): 5789
Parent Vector: None
Empty Vector: None
Properties: bacterial expression, multiple cloning site, with tag/fusion/marker
Author Name: Rutgers University
Gaetano T. Montleione
Thomas B. Acton
Stephen Anderson
Northeast Structural Genomics Consortium
PSI:Biology
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 origin 3944 4626
promoter T7 T7 promoter 541 560
protease cleavage site TEV TEV protease cleavage site 387 404
repressor binding site lac operator Lac operator 519 541
repressor protein gene LacI Lac repressor 3344 3520
selectable marker AmpR ampicillin resistance 4724 5383
tag His N-terminal 6xHis tag 405 422
tag AviTag Avi Tag 423 466
trxn termination sequence T7 term T7 terminator 217 263

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Experimental
  • Organism
  • Genome

Gene

 

Gene Symbol : ESR1
Symbol Nomenclature : ESR1
SYNONYM : ER; ESR; ESRA; ESTRR; Era; NR3A1
Designation : ER-alpha|estradiol receptor|estrogen nuclear receptor alpha|estrogen receptor|estrogen receptor alpha 3*,4,5,6,7*/822 isoform|estrogen receptor alpha E1-E2-1-2|estrogen receptor alpha E1-N2-E2-1-2|estrogen receptor alpha delta 3*,4,5,6,7*,8*/941 isoform|estrogen receptor alpha delta 3*,4,5,6,7*/819-2 isoform|estrogen receptor alpha delta 4 +49 isoform|estrogen receptor alpha delta 4*,5,6,7*/654 isoform|estrogen receptor alpha delta 4*,5,6,7,8*/901 isoform|nuclear receptor subfamily 3 group A member 1
Full Nomenclature : estrogen receptor 1
GENEID : 2099
Locus Tag : RP1-130E4.1
HGNC : 3467
MIM : 133430
Vega : OTTHUMG00000016103
Target GenBank: None

 

Pathways

NCI : AP-1 transcription factor network
NCI : ATF-2 transcription factor network
NCI : FOXA1 transcription factor network
NCI : FOXM1 transcription factor network
NCI : LKB1 signaling events
NCI : Plasma membrane estrogen receptor signaling
NCI : Regulation of nuclear SMAD2/3 signaling
NCI : Regulation of Telomerase
NCI : Signaling events mediated by HDAC Class II
NCI : Signaling mediated by p38-alpha and p38-beta
NCI : Validated nuclear estrogen receptor alpha network
Reactome : Gene Expression
Reactome : Generic Transcription Pathway
Reactome : Nuclear Receptor transcription pathway
Reactome : Nuclear signaling by ERBB4
Reactome : Signal Transduction
Reactome : Signaling by ERBB4
WikiPathway : Estrogen signaling pathway
WikiPathway : Integrated Breast Cancer Pathway
WikiPathway : Leptin signaling pathway
WikiPathway : Muscle cell TarBase
WikiPathway : Nuclear Receptors

Protein

Structural Annotation Wiki : HR4801C
UniProt : P03372
HPRD : 00589

Clone

Cloning Information : 678450

Experimental

TargetTrack - Experimental data : HR4801C

Organism


TAX_ID : 9606
Species Specific ID: 2099

Genome

Chromosome : 6
Map Location : 6q25.1
Ensembl : ENSG00000091831