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GLI3 (Homo sapiens) in pET15Avi6HT_NESG

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Explanation of Terms


Gene: Gene Symbol:  GLI3
Gene Name:  GLI family zinc finger 3
Sequence             
Original Clone ID: None
Keyword: targeted domain
Species: Homo sapiens
Type: cDNA
Vector Name: pET15Avi6HT_NESG               Format:  FUSION
Source: Rutgers University
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: Stephen Anderson
Elliot Campbell
Rutgers University
Protein Capture Reagents Program
HsCD00651961

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AVAILABLE
No restriction
Special MTA: SPTA

 

Insert sequence: 471nts        
Open reading frame : 1 to 471

GAGACAAACTGCCACTGGGAAGGCTGCGCGAGGGAGTTCGACACCCAAGAGCAGCTTGTG
CACCATATAAATAACGACCATATTCATGGAGAGAAGAAGGAGTTCGTGTGCAGGTGGCTG
GACTGCTCAAGAGAGCAGAAACCCTTCAAAGCCCAGTATATGTTGGTAGTGCATATGAGA
AGACACACGGGCGAGAAGCCTCACAAATGCACTTTTGAAGGTTGCACAAAGGCCTACTCG
AGACTAGAAAACTTGAAAACACACTTGAGATCTCACACTGGAGAGAAACCATACGTCTGT
GAGCACGAAGGTTGCAACAAGGCTTTCTCAAATGCCTCTGATCGCGCCAAACACCAAAAC
AGAACGCATTCCAATGAGAAACCATATGTGTGCAAAATCCCAGGCTGCACTAAGCGTTAC
ACAGACCCAAGCTCCCTCCGGAAACATGTGAAGACAGTGCATGGCCCATGA


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pET15Avi6HT_NESG

pET15Avi6HT_NESG in advanced viewed

Synonyms: ''
Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Bacterial expression vector with T7 lac promoter, N-terminal TEV-cleavable Avi-tag and 6XHis-tag; ampicillin resistance in bacteria; restriction enzyme cloning
Comments: None
Size (bp): 5789
Parent Vector: None
Empty Vector: None
Properties: bacterial expression, multiple cloning site, with tag/fusion/marker
Author Name: Rutgers University
Gaetano T. Montleione
Thomas B. Acton
Stephen Anderson
Northeast Structural Genomics Consortium
PSI:Biology
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 origin 3944 4626
promoter T7 T7 promoter 541 560
protease cleavage site TEV TEV protease cleavage site 387 404
repressor binding site lac operator Lac operator 519 541
repressor protein gene LacI Lac repressor 3344 3520
selectable marker AmpR ampicillin resistance 4724 5383
tag His N-terminal 6xHis tag 405 422
tag AviTag Avi Tag 423 466
trxn termination sequence T7 term T7 terminator 217 263

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Experimental
  • Organism
  • Genome

Gene

 

Gene Symbol : GLI3
Symbol Nomenclature : GLI3
SYNONYM : ACLS; GCPS; GLI3-190; GLI3FL; PAP-A; PAPA; PAPA1; PAPB; PHS; PPDIV
Designation : GLI-Kruppel family member GLI3|glioma-associated oncogene family zinc finger 3|oncogene GLI3|transcriptional activator GLI3|zinc finger protein GLI3
Full Nomenclature : GLI family zinc finger 3
GENEID : 2737
HGNC : 4319
MIM : 165240
Vega : OTTHUMG00000023630
Target GenBank: None

 

Pathways

NCI : Hedgehog signaling events mediated by Gli proteins
Panther : Hedgehog signaling pathway
WikiPathway : Endochondral Ossification
WikiPathway : Hedgehog Signaling Pathway

Protein

Structural Annotation Wiki : HR7118B
UniProt : P10071
HPRD : 01313

Clone

Cloning Information : 678570

Experimental

TargetTrack - Experimental data : HR7118B

Organism


TAX_ID : 9606
Species Specific ID: 2737

Genome

Chromosome : 7
Map Location : 7p13
Ensembl : ENSG00000106571