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APIP (Homo sapiens) in pET15_NESG (His-tagged bacterial expression vector)

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Explanation of Terms


Gene: Gene Symbol:  APIP
Gene Name:  APAF1 interacting protein
Sequence             
Original Clone ID: HR8970-18-233-15.20
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pET15_NESG               Format:  CLOSED
Source: Northeast Structural Genomics Consortium
Description: None
Comments: None
Mutation/
Discrepancy :
No / Yes
Publications: None
Authors: Northeast Structural Genomics Consortium
Rutgers University
PSI:Biology
HsCD00664138

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AVAILABLE
No restriction
Special MTA: PSI/NESG

 

Insert sequence: 651nts         Open reading frame : 49 to 699

AGAAGGAGATATACCATGGGCCATCACCATCACCATCACAGCCATATGGCGCAGGACAAG
GAGCATCCAAGATACCTGATCCCAGAACTTTGCAAACAGTTTTACCATTTAGGCTGGGTC
ACTGGGACTGGAGGAGGAATTAGCTTGAAGCATGGCGATGAAATCTACATTGCTCCTTCA
GGAGTGCAAAAGGAACGAATTCAGCCTGAAGACATGTTTGTTTGTGATATAAATGAAAAG
GACATAAGTGGACCTTCGCCATCGAAGAAGCTAAAAAAAAGCCAGTGTACTCCTCTTTTC
ATGAATGCTTACACAATGAGAGGAGCAGGTGCAGTGATTCATACCCACTCTAAAGCTGCT
GTGATGGCCACCCTTCTCTTTCCAGGACGGGAGTTTAAAATTACACATCAAGAGATGATA
AAAGGAATAAAGAAATGTACTTCCGGAGGGTATTATAGATATGATGATATGTTAGTGGTA
CCCATTATTGAGAATACACCTGAGGAGAAAGACCTCAAAGATAGAATGGCTCATGCAATG
AATGAATACCCAGACTCCTGTGCAGTACTGGTCAGACGTCATGGAGTATATGTGTGGGGG
GAAACATGGGAGAAGGCCAAAACCATGTGTGAGTGTTATGACTATTTATTTGATATTGCC
GTATCAATGAAGAAAGTAGGACTTGATCCTTCACAGTAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
a324c,T108T
                                

5' Linker Sequence:
ATGGGCCATCACCATCACCATCACAGCCATATG

3' Linker Sequence:
CTCGAGATCCGG

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: ProteinExpressed: Protein_Confirmed
ProteinPurified: Tested_Not_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in HR8970
Recommended expression in: E. coli BL21
       DyNA Vector Map

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Vector Name: pET15_NESG

pET15_NESG in advanced viewed

Synonyms: pET15, pET15-modified, pET15Cc, pET15_NESG
Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Bacterial (E. coli) expression vector with T7 lac promoter, adds N-terminal 6x His tag; amp resistance; restriction enzyme cloning.
Comments: Modified Novagen pET vector-common MCS
Size (bp): 5723
Parent Vector: None
Empty Vector: EvNO00336943
Properties: bacterial expression, in vitro transcription, multiple cloning site, with tag/fusion/marker
Author Name: Northeast Structural Genomics Consortium
Gaetano T. Montleione
Jessica Bearden
Publications: PMID: 15808222
Title: Robotic cloning and Protein Production Platform of the Northeast Structural Genomics Consortium

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS MCS (includes SphI, XbaI, EcoRI, BamHI, XhoI) 327 452
bacterial origin ori ColE1 origin of replication 3881 4563
gene fragment ROP repressor of primer ROP sequence 3281 3457
primer T7 forward T7 forward sequencing primer 481 496
primer T7 reverse T7 reverse sequencing primer 257 275
promoter T7 T7 promoter 478 497
repressor binding site lacO lacO regulatory sequence 456 478
selectable marker AmpR ampicillin resistance gene 4661 5230
tag His N-terminal 6x His tag 387 404
trxn termination sequence T7 term T7 termination sequence 257 275

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Experimental
  • Organism
  • Genome

Gene

 

Gene Symbol : APIP
Symbol Nomenclature : APIP
SYNONYM : APIP2; CGI29; MMRP19; hAPIP
Designation : APAF1-interacting protein|MTRu-1-P dehydratase|methylthioribulose-1-phosphate dehydratase|probable methylthioribulose-1-phosphate dehydratase
Full Nomenclature : APAF1 interacting protein
GENEID : 51074
Locus Tag : CGI-29
GI : 166235186
GenBank Accession : NM_015957
HGNC : 17581
MIM : 612491
Vega : OTTHUMG00000166455
Target GenBank: None

 

Pathways

Reactome : Metabolism
Reactome : Metabolism of amino acids and derivatives
Reactome : Metabolism of polyamines
Reactome : Methionine salvage pathway
Reactome : Sulfur amino acid metabolism

Protein

Structural Annotation Wiki : HR8970
UniProt : Q96GX9
HPRD : 14725

Clone

Cloning Information : 688279
Original Clone ID : HR8970-18-233-15.20

Experimental

TargetTrack - Experimental data : HR8970

Organism


TAX_ID : 9606
Species Specific ID: 51074

Genome

Chromosome : 11
Map Location : 11p13
Ensembl : ENSG00000149089