Gene:
Gene Symbol:
IST1
Gene Name:
increased sodium tolerance 1 homolog (yeast)
Original Clone ID:
WISP 14-18
Keyword:
"Mutation:c162a; c495t; g498c; t501c; a400g; g402c, Mutation:K134D, IST1 K134D Sundquist Lab Internal ID corresponding to this clone = WISP 14-18"
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pQCYFPxIN
Format:
FUSION
Source:
University of Utah
Description:
None
Comments:
None
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
University of Utah
Wesley I. Sundquist
Leremy A Colf
Wesley I. Sundquist
Leremy A Colf
HsCD00671544
Price:
Login for Pricing
AVAILABLE
No restriction
Special MTA: SPTA
Special MTA: SPTA
Insert sequence: 1101nts
Open reading frame: 1 to 1101
ATGCTGGGCTCTGGATTTAAAGCTGAGCGCTTAAGAGTGAATTTGAGATTAGTCATAAAT
CGCCTTAAACTATTGGAGAAAAAGAAAACGGAACTGGCCCAGAAAGCAAGGAAGGAGATT
GCTGACTATCTGGCTGCTGGGAAAGATGAACGAGCTCGGATACGTGTGGAGCACATTATC
CGGGAAGACTACCTCGTGGAGGCCATGGAGATCCTGGAGCTGTACTGTGACCTGCTGCTG
GCTCGGTTTGGCCTTATCCAGTCTATGAAGGAACTAGATTCTGGTCTGGCTGAATCTGTG
TCTACATTGATCTGGGCTGCTCCTCGACTCCAGTCAGAAGTGGCTGAGTTGAAAATAGTT
GCTGATCAGCTCTGTGCCAAGTATAGCAAGGAATATGGCGACCTATGTAGGACCAACCAG
ATTGGAACTGTGAATGACAGGCTAATGCACAAGCTGAGTGTGGAAGCCCCACCCAAAATC
CTGGTGGAGAGATATCTCATCGAAATTGCAAAGAATTACAACGTACCCTATGAACCTGAC
TCTGTGGTCATGGCAGAAGCTCCTCCTGGGGTAGAGACAGATCTTATTGATGTTGGATTC
ACAGATGATGTGAAGAAAGGAGGCCCTGGAAGAGGAGGGAGTGGTGGCTTCACAGCACCA
GTTGGTGGACCTGATGGAACGGTGCCAATGCCCATGCCCATGCCCATGCCTATGCCATCT
GCAAATACGCCTTTCTCATATCCACTGCCAAAGGGACCATCAGATTTCAATGGACTGCCA
ATGGGGACTTATCAGGCCTTTCCCAATATTCATCCACCTCAGATACCAGCAACTCCCCCA
TCGTATGAATCTGTAGATGACATTAATGCTGATAAGAATATCTCTTCTGCACAGATTGTT
GGTCCTGGACCCAAGCCAGAAGCCTCTGCAAAGCTTCCTTCCAGACCTGCAGATAACTAT
GACAACTTTGTCCTACCAGAGTTGCCATCTGTGCCAGACACACTACCAACTGCATCTGCT
GGTGCCAGCACCTCAGCATCTGAAGACATTGACTTTGATGATCTTTCCCGGAGGTTTGAA
GAGCTGAAAAAGAAAACATAG
Protein sequence predicted based on nucleotide sequence
MLGSGFKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHII REDYLVEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIV ADQLCAKYSKEYGDLCRTNQIGTVNDRLMHKLSVEAPPKILVERYLIEIAKNYNVPYEPD SVVMAEAPPGVETDLIDVGFTDDVKKGGPGRGGSGGFTAPVGGPDGTVPMPMPMPMPMPS ANTPFSYPLPKGPSDFNGLPMGTYQAFPNIHPPQIPATPPSYESVDDINADKNISSAQIV GPGPKPEASAKLPSRPADNYDNFVLPELPSVPDTLPTASAGASTSASEDIDFDDLSRRFE ELKKKT*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pQCYFPxIN
Synonyms:
''
Description:
Retroviral mammalian expression vector with an SV40 promoter and N-terminal YFP; ampicillin resistance in bacteria; BamHI, EcoRI restriction enzyme cloning
Comments:
3' primer is a forward primer within the insert. There is an IRES following the stop codon and cloning site at the 3' end that is refractory to sequencing.
Size (bp):
8090
Parent Vector:
None
Properties:
fluorescent marker, mammalian expression, multiple cloning site, retroviral vector, with tag/fusion/marker
Author Name:
BD Biosciences Clontech
Publications:
PMID:
20616062
Title: Human ESCRT-III and VPS4 proteins are required for centrosome and spindle maintenance.
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 bacterial origin
3101
3783
primer
forward primer
YFP forward sequencing primer CATCAAGGTGAACTTCAAGA
7832
7851
promoter
SV40
SV40 promoter
2630
2826
selectable marker
AmpR
ampicillin resistance
3881
4540
tag
YFP
N-terminal YFP
7353
7796
